EP0364621A1 - Dispositif pour pipetter - Google Patents

Dispositif pour pipetter Download PDF

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Publication number
EP0364621A1
EP0364621A1 EP88117553A EP88117553A EP0364621A1 EP 0364621 A1 EP0364621 A1 EP 0364621A1 EP 88117553 A EP88117553 A EP 88117553A EP 88117553 A EP88117553 A EP 88117553A EP 0364621 A1 EP0364621 A1 EP 0364621A1
Authority
EP
European Patent Office
Prior art keywords
pipetting device
actuating element
button
engagement
spindle
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Granted
Application number
EP88117553A
Other languages
German (de)
English (en)
Other versions
EP0364621B1 (fr
Inventor
Horst Sabloewski
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Eppendorf SE
Original Assignee
Eppendorf Netheler Hinz GmbH
Eppendorf Geraetebau Netheler and Hinz GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Eppendorf Netheler Hinz GmbH, Eppendorf Geraetebau Netheler and Hinz GmbH filed Critical Eppendorf Netheler Hinz GmbH
Priority to EP88117553A priority Critical patent/EP0364621B1/fr
Priority to ES198888117553T priority patent/ES2035210T3/es
Priority to DE8888117553T priority patent/DE3875117D1/de
Priority to AT88117553T priority patent/ATE81032T1/de
Priority to JP1274786A priority patent/JPH0611407B2/ja
Priority to US07/425,791 priority patent/US5012682A/en
Publication of EP0364621A1 publication Critical patent/EP0364621A1/fr
Application granted granted Critical
Publication of EP0364621B1 publication Critical patent/EP0364621B1/fr
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops

Definitions

  • the invention relates to a pipetting device with a housing, at the upper end of which protrudes an actuating element which can be pressed in against spring action and which is connected to a piston which can be moved in a cylinder in order to move an air volume which cylinder has an opening of a connecting attachment, in particular a connecting cone. for pluggable pipette tips which are open at the top and at the bottom, settings of the pipetting volume being provided by rotating the actuating element.
  • the invention is based in particular on a pipetting device according to DE-P 25 49 477, in which special holding devices, also as a magnet arrangement, are provided and in the lower region a piston rod can be moved within a cylinder in order to with respect to the lower, open housing end, in which the cylinder ends, to move an air volume in order to draw in or dispense a liquid sample without contact to the piston.
  • cam-like stops which are offset around the circumference and which can be arranged at different heights are arranged for volume adjustment. These stops can be set using a mechanical locking device on the actuating element, in particular an actuating button for adjustment in abutment with an abutment, so that a selected pipetting volume can thereby be predetermined.
  • the arrangement of springs is also known from the known embodiment in order to produce the connection of interacting parts, unless a magnet arrangement is present.
  • This embodiment works unsafe per se, especially since it allows longitudinal grooves on the outer surface of the sleeve to interact with a closure tip which is pressed by a leaf spring. So there is a catch-like snap connection, which on the one hand has no fine adjustment, on the other hand also causes noise.
  • a locking device is known on a pipette with adjustable volume, which has spherical projections which cooperate with corresponding recesses, which are arranged in another part, so that volume adjustment is possible.
  • This is also a coarse screen, the adjustment of which causes noise and does not allow fine adjustment.
  • the invention has for its object to provide a pipetting device of the type mentioned, which allows fine adjustment, but a safe setting and easy, but also reliable operation are guaranteed.
  • the button-like actuating element is also provided as a threaded insert for solving a detachable locking element engagement between a spindle and a fixed abutment and by rotating the button-like actuating element in a spindle drive of a piston rod for the piston of the piston in a manner known per se is adjustable.
  • the detachable locking engagement in connection with a stepless adjustability creates the prerequisite for a particularly inexpensive pipetting device that is fixed in the setting, but can be freely selected in practice. If a locking engagement is mentioned, these are detents of the finest kind.
  • At least one of the locking elements is advantageously arranged distributed around the circumference of the central axes of the pipetting device.
  • any circumferential setting can be realized. It is preferred that both locking elements are arranged distributed around the circumference, because then - which is advantageous for particularly fine settings - a radially symmetrical intervention is reserved, which, however, can be canceled by axial movement according to the above explanations or can be engaged again by opposite axial movement.
  • the locking element engagement is particularly advantageously carried out with two toothed rings which are arranged on the parts of the pipetting device which are to be moved relative to one another.
  • the use of sprockets has the advantage of being relatively easy to manufacture with the finest division.
  • Such a locking element engagement works in the preferred embodiment with a tooth pitch that allows the smallest circumferential rotation steps, but in another expedient embodiment is also directly matched to a volume setting. An adjustment by a tooth pitch would bring about the smallest selectable volume adjustment.
  • Another expedient version provides a tooth depth of approximately 1.5 mm for reliable tooth engagement. This also enables a largely fine tooth division.
  • a toothing engagement is provided in the axial direction with a toothed ring having teeth pointing upwards, into which a toothed ring engages on the button carrier from above. This is an advantageous embodiment.
  • a toothing engagement is provided in a toothed ring designed in the radial direction, to which a corresponding counter toothed ring on the spindle is assigned so that it can be lifted out in the axial direction.
  • a friction or slip clutch is arranged between the knob-like actuating element and the spindle, which allows an actuation element rotation with existing tooth engagement. This protects the fine toothing or protects it from destruction, even if the button-shaped actuating element is rotated at will.
  • axially removable locking elements also have locking profiles as teeth, which are designed to be able to be overcome in order to maintain the engagement.
  • latching profiles are designed with mutual thickening at their ends in the sense of the engagement and lifting direction and that these thickenings are arranged on elastic tooth-like webs.
  • the thickenings are expediently spherical beads.
  • the thickenings have a conical shape with opposite slopes with respect to the parts that come into engagement with one another. Mutual interventions can also be achieved in this way, provided that the thickenings are arranged on elastic webs.
  • a piston rod is provided between the piston and the upper end of the button-shaped actuating element and has a stop on the spindle, which limits the maximum position of the piston rod with respect to the spindle and in the button-like actuating element. This stop, too, is protected against damage from the friction or slip clutch when it is engaged.
  • a further advantageous embodiment is that the friction or slip clutch between the button-like actuating element and the toothed carrier of the button-like actuating element is arranged in the circumferential direction and has an elastic element with indentations, in which engage corresponding elastic engagement elements of the counter-coupling element, one Coupling element designed as a button carrier is guided for tooth engagement and the other coupling element is arranged on the rotatable button-like actuating element.
  • a carrier which is guided in a rotationally fixed manner in the housing has axially directed spring elements which engage like tongues with engagement lugs in ring grooves of the piston rod, the engagement between different ring grooves being variable as a function of a pull-out position of the button-like actuating element.
  • the latter is designed in two parts with particular advantage, but both parts are non-rotatably connected to one another and an elastic insert of the upper part is provided on the towering piston rod with spring elements which can be spread outwards and which engage under abutment parts in the carrier of the button-like actuating element.
  • the friction or slip clutch consists of groove-like incisions on an upwardly projecting cylindrical extension of the intermediate element and inwardly directed webs on downwardly directed edge parts of the cover shell.
  • teeth of the load elements are lamellar and are provided for a lateral evasive movement.
  • the locking profiles are formed on one side.
  • the profiles protrude on one gear rim and are designed as negative profiles on the other gear rim.
  • Another particularly expedient embodiment provides that the two ring gears are arranged on circumferential circles with different radii, with opposing beads overlapping on the respective toothings and providing elastic resilience of the teeth, in particular also in the radial direction.
  • Figures 1 a and 1 b are collectively referred to as Fig. 1.
  • Fig. 1 the pipetting device is only partially shown, with the upper part of the housing 1, from which an actuating element 2 protrudes in the middle upwards as a push and rotary lever or correspondingly actuatable button.
  • stepless adjustment provides that in the housing 1 a threaded insert 3 by screwing firmly in the circumferential and in the axial direction cannot be relocated, namely by a screw thread 3 'and in its internal thread 4 an external thread 5 of a spindle 6 can be rotated .
  • the piston rod 7 passes through the spindle in the axial direction without a rotary connection.
  • This piston rod 7 abuts with its upper end 8 on the cover 9 of the button-like actuating element 2, which is initially referred to as such up to a lower edge 10.
  • the actuating element 2 is connected in one piece in the axial direction to the button carrier 11 by expandable spring elements 12, 13 on the cover shell, the outwardly directed contact pieces 14, 15 of the spring elements at the same time ensure the cohesion between the parts 9 and 11 because the abutment parts 16, 17 are arranged on the button holder 11.
  • the button carrier 11 has a guide 18 inside made of spring arms 19, 20, as can also be seen in FIG. 2, and which are delimited from one another by longitudinal slots 48.
  • the piston rod 7 passes through this guide. It has two circumferential recesses 21, 22 one above the other at a distance. Inward engagement shoes 23, 24 engage in these circumferential recesses at the lower ends of the spring arms 19, 20. It can be seen that the piston rod can thus in each case be rotated but can be changed in the axial direction in two different positions relative to the actuating element 2.
  • a stop disk 25 is also arranged on the piston rod 7 and forms a stop between the piston rod 7 and the lower edge of the spring arms 19, 20 or the engagement shoes 23, 24 arranged on them. This abutment prevents the piston rod 7 from migrating too far upwards with respect to the actuating element 2, or it forms a stop for pressing the piston rod 7 downward when the actuating element 2 is pressed in again.
  • a spring abutment 27 for a compression spring 28 is arranged on the piston rod just below the upper head, which is supported on the other hand via a washer 29 on the intermediate web 30 of the button carrier 11.
  • This is only a uniform and elastic engagement standing version of the actuating element 2 created by pressing the spring elements 12, 13 after their insertion against the stops of the abutment parts 16, 17.
  • the cover shell 9 can be pulled out together with the actuating element 2, taking the button carrier 11 with it, so that the engagement shoes 24 are removed from the peripheral recess 21 and can be latched into the peripheral recess 22. This is achieved in that a stop 42 bears against the piston rod 7 at the lower end of the spindle 6.
  • the inwardly directed grooves 36, 37 on the driver 38 are connected in a rotationally fixed manner to the spindle 6, so that through the engagement of the webs 34, 35 the spindle is taken along when the actuating element 2 is rotated, as long as the friction clutch 73 remains engaged.
  • the inward grooves in the axial direction are longer than the extent of the teeth 31 and counter teeth 32 in the axial direction. This means that the slewing ring is retained even when these teeth are disengaged.
  • the locking disk 33 is displaceable relative to the driver 38 and has projections 40 which engage in grooves 39 in the housing 1, so that the locking disk 33 can be pressed downward along the housing 1. Between the threaded insert 3 and the locking disk 33 there is a compression spring 41 which tends to push the locking disk upwards. As a result, the engagement between the toothed parts 31, 32 is maintained. So that the locking disc 33 is rotatably but resiliently held in the axial direction, but only to the extent that the engagement of the projections 40 with the downwardly angled legs remain in the grooves 39, even if the button-like actuating element is pulled out until the stop disk 25 abuts the lower edges of the engagement shoes 23, 24.
  • FIG. 3 shows the toothing 31, the lugs 46, 47 being shown at the same time. Furthermore, the inwardly directed webs 34, 35 are shown, it being apparent from FIG. 3 that corresponding parts which are offset by 90 ° are present, the webs 70, 71 engaging in inwardly directed grooves of the driver 38.
  • This driver of which a side view is shown in FIG. 7, has a circumferential configuration which results from FIG. 3, wherein it can be seen that a configuration is provided with regard to the circumference in the sense of the stability of the arrangement.
  • the actuating element 2 can be pulled upwards, the engagement shoes 23, 24 engaging in the upper peripheral recess.
  • the outwardly directed lugs 46, 47 move upwards in grooves in an insert 51.
  • the insert 51 is supported and held on a step 53 of the housing 1 with an extension projecting outwards and forming a shoulder 52.
  • the holder is carried out by the part 54, not shown in FIG. 1, on the top of the housing.
  • the piston rod 7 ends in a rotationally fixed manner on a component 55.
  • the continuing piston rod or the piston 56 is arranged there, which according to the mentioned DE-P 25 49 477 then ultimately also extends with extensions into the lower connecting cone or into one that continues Channel engages in order to bring about the suction effect or expression via an air volume.
  • the internal thread 57 of the housing now gives the possibility of connecting further housing parts, and in the area 58 there can be special holding devices which, according to DE-P 25 49 477, are designed as overcomeable holding devices, in particular in the embodiment as a separable magnet arrangement. As a result, a certain setting is infinitely defined in a simple manner with the advantageous magnet arrangements.
  • the piston rod 7 can be moved downward from the lower edge 43 of the spindle, but not upwards. If the pipetting volume is to be reduced from the starting position shown, which represents the largest pipetting volume, the actuating element 2 can be pulled upwards so that the engagement shoes 23, 24 engage in the circumferential recess 22. Thus, the toothing at 31, 32 is disengaged and the spindle can be freely rotated by rotating the actuating element 2 in the direction of the downward movement, the piston rod 7 being carried downward. After sufficient rotation, the actuating element is pressed in again, so that the engagement shoes 23, 24 engage in the peripheral recess 21.
  • the side view reveals the outer cylinder 60, which is also present according to FIG. 1 and can be made transparent. It has the inward step 61 on top, which is on the upward step Flange 62 of the button holder 11 supports.
  • the cover shell 9 is held on the upper, outwardly directed step 63 by the engagement of the spring elements 12, 13.
  • the intermediate element 59 has on the inside of the upper step 63 an upwardly projecting cylindrical extension 64 (FIG. 9), which has groove-like incisions 65, 66 going around the circumference. This protruding projection is elastic.
  • the slip clutch advantageously has an elastic element 64 with indentations into which corresponding elastic engagement elements 67, 68 of the mating clutch element engage, one clutch element 11 leading to tooth engagement and the other clutch element being arranged on the rotatable knob-like actuating element 2.
  • a support 11 which is guided in a rotationally fixed manner in the housing, also expediently has axially directed spring elements 19, 20 which engage like tongues with engagement shoes 24, 25 in annular grooves 21, 22 of the piston rod 7, the engagement between different annular grooves 21, 22 depending on an extended position of the button-like actuator 2 is changeable.
  • both parts are connected to one another in a rotationally fixed manner, but there is an elastic insert of the upper part on the projecting piston rod 7 with spring elements 12, 13 which can be elastically expanded outwards, which abutment parts 16, 17 in the carrier 11 of the button-like Reach in under the actuator.
  • the axially movable, guided in the housing locking disc is under spring pressure upwards, namely to rest against a counter bearing at the lower edge of the insert 51 in the housing.
  • FIG. 10 shows another embodiment of the lower part of the individual part drawn in FIG. 4. It can be seen that this lower part extends to the lower opening 74. Inside the wall 75, inwardly directed teeth 76 are arranged at the opening edge, which can be placed on the ring gear 32, which in this case has an exactly corresponding additional profile in its radial arrangement, so that a particularly fine tooth division is possible.
  • the mutually engaging tooth parts which are referred to as toothing and counter toothing above with 31 and 32, are designed in a special way.
  • the toothing 31 is located on the lower edge of the button carrier 11, while the counter toothing 32 is arranged on the insert or the locking disk 33.
  • FIGS. 12 and 13 each show a section of the locking disk 33 with an upwardly directed, specially designed counter-toothing and also the downward toothing on the button carrier 11, of which only a section is drawn.
  • the teeth 77, 78, 79 ... corresponding to the counter toothing have a spherical thickening 80, 81; 82, 83 have, on the teeth 84 to 87 corresponding to the toothing 31, a corresponding bead-like thickening 88 to 91 is arranged, for example, only on one side.
  • FIG. 11 shows this toothing arrangement in a top view, specifically in section along the line XI-XI in FIG. 12.
  • the teeth 84 to 87 coming from above with the one-sided thickening 88 to 91 can be seen, as is the spherical thickening 80 to 83, which is arranged on the lower teeth 77 to 79.
  • FIG. 13 shows a representation corresponding to FIG. 12 in a detail.
  • the teeth 92, 93, 94 ... corresponding to the toothing 31 are conical and are arranged in such a way that they can elastically deflect at their roots 95 to 97.
  • the teeth 98 to 100 of the counter-toothing are designed on the locking disk 33, a tooth spacing or tooth pitch being provided in such a size that a lateral evasive movement is possible during the transition with a snap action when inserting or loosening the toothing.
  • the profiling protruding toward the tooth head is only arranged on one side in such a way that there are always two such profiles opposite, while the other side of the tooth can be profiled in the usual way.
  • the head edges are rounded, so that an evasive movement is brought about when the parts are moved towards one another in the axial direction or are separated from one another.
  • FIGS. 11 to 13 show designs in which the teeth are on the same circumferential circles.
  • the two ring gears are located on circumferential circles with different radii in such a way that they still touch. It is then included here that opposing beads overlap on the respective toothings and accordingly engage with elastic resilience of the teeth, in particular also in the radial direction, with the generation of a cogging torque.
  • the latching means can also be designed in such a way that a protruding profile is formed on one sprocket and corresponding negative profiles are formed on the other sprocket, so that the effort for engagement is reduced.
EP88117553A 1988-10-21 1988-10-21 Dispositif pour pipetter Expired - Lifetime EP0364621B1 (fr)

Priority Applications (6)

Application Number Priority Date Filing Date Title
EP88117553A EP0364621B1 (fr) 1988-10-21 1988-10-21 Dispositif pour pipetter
ES198888117553T ES2035210T3 (es) 1988-10-21 1988-10-21 Dispositivo para accionar pipetas.
DE8888117553T DE3875117D1 (de) 1988-10-21 1988-10-21 Pipettiervorrichtung.
AT88117553T ATE81032T1 (de) 1988-10-21 1988-10-21 Pipettiervorrichtung.
JP1274786A JPH0611407B2 (ja) 1988-10-21 1989-10-20 ピペット装置
US07/425,791 US5012682A (en) 1988-10-21 1989-10-23 Pipetting device

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP88117553A EP0364621B1 (fr) 1988-10-21 1988-10-21 Dispositif pour pipetter

Publications (2)

Publication Number Publication Date
EP0364621A1 true EP0364621A1 (fr) 1990-04-25
EP0364621B1 EP0364621B1 (fr) 1992-09-30

Family

ID=8199478

Family Applications (1)

Application Number Title Priority Date Filing Date
EP88117553A Expired - Lifetime EP0364621B1 (fr) 1988-10-21 1988-10-21 Dispositif pour pipetter

Country Status (6)

Country Link
US (1) US5012682A (fr)
EP (1) EP0364621B1 (fr)
JP (1) JPH0611407B2 (fr)
AT (1) ATE81032T1 (fr)
DE (1) DE3875117D1 (fr)
ES (1) ES2035210T3 (fr)

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* Cited by examiner, † Cited by third party
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DE4014333A1 (de) * 1990-05-04 1991-11-28 Eppendorf Geraetebau Netheler Pipettiervorrichtung
WO2006030201A1 (fr) 2004-09-13 2006-03-23 Microsample Ltd. Procede et appareil permettant d'echantillonner et d'analyser des fluides
DE102004055562A1 (de) * 2004-11-18 2006-06-01 Eppendorf Ag Handhabbares Dosiersystem
WO2010086380A1 (fr) 2009-01-30 2010-08-05 Pronota N.V. Biomarqueur de diagnostic, prédiction et/ou pronostic d'une insuffisance cardiaque aigue et ses utilisations
WO2011048173A1 (fr) 2009-10-21 2011-04-28 Pronota N.V. Mcam en tant que biomarqueur pour l'homéostasie des fluides
WO2011128357A2 (fr) 2010-04-13 2011-10-20 Pronota N.V. Biomarqueurs de troubles liés à l'hypertension pendant la grossesse
EP2626134A3 (fr) * 2012-02-07 2013-11-06 Eppendorf AG Pipette
EP2924439A1 (fr) 2010-03-26 2015-09-30 Mycartis N.V. Ltbp2 comme biomarqueur pour la prediction ou le pronostic de la mortalite

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4209620C1 (de) * 1992-03-25 1993-12-16 Eppendorf Geraetebau Netheler Verfahren zur Korrektur des Volumenfehlers ïV bei einem Pipettiersystem
US5804144A (en) * 1993-04-21 1998-09-08 Labsystems Oy Piston pipette
US5650124A (en) * 1995-07-24 1997-07-22 Gilson; Warren E. Adjustable pipette
US5849248A (en) * 1996-03-05 1998-12-15 Rainin Instrument Co., Inc. Adjustable volume pipette with improved volume adjustment lock mechanism
US6428750B1 (en) * 2000-02-17 2002-08-06 Rainin Instrument, Llc Volume adjustable manual pipette with quick set volume adjustment
FR2807345B1 (fr) * 2000-04-07 2002-07-12 Gilson Sa Pipette de prelevement a demontage simplifie
FR2807344B1 (fr) * 2000-04-07 2002-12-06 Gilson Sa Pipette de prelevement a mecanisme de reglage du volume de prelevement
FR2807343B1 (fr) * 2000-04-07 2002-12-06 Gilson Sa Pipette de prelevement munie de moyens de reglage du volume a prelever
FR2807342B1 (fr) * 2000-04-07 2002-07-12 Gilson Sa Pipette de prelevement a ejecteur de cone
EP1438138B1 (fr) * 2001-10-16 2010-09-08 Matrix Technologies Corporation Pipette manuelle
US7284454B2 (en) * 2004-05-27 2007-10-23 Matrix Technologies Corporation Hand held pipette
US20060027033A1 (en) * 2002-10-16 2006-02-09 Richard Cote Hand-held pipette employing voice recognition control
US7175813B2 (en) * 2003-12-31 2007-02-13 Rainin Instrument, Llc Volume adjustable manual pipette with quick set volume adjustment
US7381371B2 (en) * 2004-01-16 2008-06-03 Heathrow Scientific Llc Pipette device with pivotable nozzle assembly
DE102005033378B4 (de) * 2005-07-16 2012-05-31 Eppendorf Ag Kolbenhubpipette
GB0605003D0 (en) * 2006-03-13 2006-04-19 Microsample Ltd Method and apparatus for piercing the skin and delivery or collection of liquids
US20090010809A1 (en) * 2007-07-03 2009-01-08 Hadjis Peter T Manual pipette filler
US20090007701A1 (en) * 2007-07-03 2009-01-08 Hadjis Peter T Pivoting pipette device
US9044749B2 (en) * 2012-02-29 2015-06-02 Eppendorf Ag Pipette
US9358538B2 (en) 2012-04-30 2016-06-07 The Regents Of The University Of Michigan High resolution pipette
FR3080050B1 (fr) * 2018-04-17 2020-03-27 Gilson Sas Dispositif de verrouillage d'une vis de reglage de volume pour un systeme de pipetage
FI3932557T3 (fi) * 2020-07-02 2023-12-28 Eppendorf Se Monikanavainen pipettipää
KR102427246B1 (ko) * 2020-09-18 2022-07-29 바디텍메드(주) 미니 파이펫

Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2330456A1 (fr) * 1975-11-05 1977-06-03 Eppendorf Geraetebau Netheler Dispositif de pipetage
US4096750A (en) * 1977-06-15 1978-06-27 Oxford Laboratories Inc. Hand-held micropipettor with fluid transfer volume adjustment mechanism
US4098125A (en) * 1977-03-30 1978-07-04 Lee Thomas E Adjustable volume pipetting device
US4165646A (en) * 1978-07-26 1979-08-28 Shapiro Justin J Adjustable micro-dispensing liquid pipet
GB2022453A (en) * 1978-06-02 1979-12-19 Suovaniemi Finnpipette Pipette of adjustable volume
EP0181957A1 (fr) * 1984-11-20 1986-05-28 HTL High-Tech Lab Herstellung und Vertrieb medizinisch technischer Erzeugnisse GmbH Pipette à dose réglable

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2946486A (en) * 1957-05-29 1960-07-26 Manostat Corp Analytical device
US3646817A (en) * 1968-10-25 1972-03-07 Bio Dynamics Inc Pipette
US3613952A (en) * 1970-04-23 1971-10-19 Cole Parmer Instr Co Fluid dispenser with adjustable stroke piston and register
US3766785A (en) * 1971-05-17 1973-10-23 Analytical Prod Automatic pipette
GB1392792A (en) * 1972-02-10 1975-04-30 Suovaniemi Osmo Antero Test tube element for use with a multiple pipette
US3827305A (en) * 1972-10-24 1974-08-06 R Gilson Adjustable pipette
DE2319175A1 (de) * 1973-04-16 1974-10-31 Oxford Lab Detail-kolbenpipette
GB1510081A (en) * 1976-03-04 1978-05-10 Atkin R Pipettes
US4141251A (en) * 1978-02-15 1979-02-27 Nichiryo Co., Ltd. Pipetting device
USRE30453E (en) * 1979-03-19 1980-12-23 Medical Laboratory Automation, Inc. Pipettes
FI60359C (fi) * 1980-06-02 1982-01-11 Suovaniemi Finnpipette Pipett
DE3137423A1 (de) * 1981-09-19 1983-03-31 Rudolf Brand GmbH & Co, 6980 Wertheim "mikroliterpipette"
FI64752C (fi) * 1982-06-29 1984-01-10 Labsystems Oy Volymreglerbar pipett
US4567780A (en) * 1984-03-12 1986-02-04 American Hospital Supply Corporation Hand-held pipette with disposable capillary
FI844320A (fi) * 1984-11-02 1986-05-03 Labsystems Oy Utspaedningsdoseringsanordning.

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2330456A1 (fr) * 1975-11-05 1977-06-03 Eppendorf Geraetebau Netheler Dispositif de pipetage
US4098125A (en) * 1977-03-30 1978-07-04 Lee Thomas E Adjustable volume pipetting device
US4096750A (en) * 1977-06-15 1978-06-27 Oxford Laboratories Inc. Hand-held micropipettor with fluid transfer volume adjustment mechanism
GB2022453A (en) * 1978-06-02 1979-12-19 Suovaniemi Finnpipette Pipette of adjustable volume
US4165646A (en) * 1978-07-26 1979-08-28 Shapiro Justin J Adjustable micro-dispensing liquid pipet
EP0181957A1 (fr) * 1984-11-20 1986-05-28 HTL High-Tech Lab Herstellung und Vertrieb medizinisch technischer Erzeugnisse GmbH Pipette à dose réglable

Cited By (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE4014333A1 (de) * 1990-05-04 1991-11-28 Eppendorf Geraetebau Netheler Pipettiervorrichtung
US5511433A (en) * 1990-05-04 1996-04-30 Eppendorf-Netherler-Hinz Gmbh Pipette device
WO2006030201A1 (fr) 2004-09-13 2006-03-23 Microsample Ltd. Procede et appareil permettant d'echantillonner et d'analyser des fluides
US8092394B2 (en) 2004-09-13 2012-01-10 Microsample Ltd. Method and apparatus for sampling and analysis of fluids
DE102004055562A1 (de) * 2004-11-18 2006-06-01 Eppendorf Ag Handhabbares Dosiersystem
DE102004055562B4 (de) * 2004-11-18 2006-09-28 Eppendorf Ag Handhabbares Dosiersystem
WO2010086380A1 (fr) 2009-01-30 2010-08-05 Pronota N.V. Biomarqueur de diagnostic, prédiction et/ou pronostic d'une insuffisance cardiaque aigue et ses utilisations
WO2011048173A1 (fr) 2009-10-21 2011-04-28 Pronota N.V. Mcam en tant que biomarqueur pour l'homéostasie des fluides
WO2011048168A1 (fr) 2009-10-21 2011-04-28 Pronota N.V. Biomarqueur pour le diagnostic, la prédiction et/ou le pronostic d'insuffisance cardiaque aiguë et utilisations de celui-ci
EP2924439A1 (fr) 2010-03-26 2015-09-30 Mycartis N.V. Ltbp2 comme biomarqueur pour la prediction ou le pronostic de la mortalite
WO2011128357A2 (fr) 2010-04-13 2011-10-20 Pronota N.V. Biomarqueurs de troubles liés à l'hypertension pendant la grossesse
EP2626134A3 (fr) * 2012-02-07 2013-11-06 Eppendorf AG Pipette

Also Published As

Publication number Publication date
EP0364621B1 (fr) 1992-09-30
JPH02174942A (ja) 1990-07-06
ES2035210T3 (es) 1993-04-16
US5012682A (en) 1991-05-07
ATE81032T1 (de) 1992-10-15
JPH0611407B2 (ja) 1994-02-16
DE3875117D1 (de) 1992-11-05

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