EP0181957A1 - Pipette à dose réglable - Google Patents

Pipette à dose réglable Download PDF

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Publication number
EP0181957A1
EP0181957A1 EP84114018A EP84114018A EP0181957A1 EP 0181957 A1 EP0181957 A1 EP 0181957A1 EP 84114018 A EP84114018 A EP 84114018A EP 84114018 A EP84114018 A EP 84114018A EP 0181957 A1 EP0181957 A1 EP 0181957A1
Authority
EP
European Patent Office
Prior art keywords
sleeve
block
handle
metering
counter
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Withdrawn
Application number
EP84114018A
Other languages
German (de)
English (en)
Inventor
W. Duszczyk
W. Sarna
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
HTL HIGH-TECH LAB HERSTELLUNG UND VERTRIEB MEDIZIN
Original Assignee
HTL High-Tech Lab Herstellung und Vertrieb medizinisch technischer Erzeugnisse GmbH
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by HTL High-Tech Lab Herstellung und Vertrieb medizinisch technischer Erzeugnisse GmbH filed Critical HTL High-Tech Lab Herstellung und Vertrieb medizinisch technischer Erzeugnisse GmbH
Priority to EP84114018A priority Critical patent/EP0181957A1/fr
Publication of EP0181957A1 publication Critical patent/EP0181957A1/fr
Withdrawn legal-status Critical Current

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Classifications

    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/02—Burettes; Pipettes
    • B01L3/021—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids
    • B01L3/0217—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type
    • B01L3/0224—Pipettes, i.e. with only one conduit for withdrawing and redistributing liquids of the plunger pump type having mechanical means to set stroke length, e.g. movable stops
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/02—Identification, exchange or storage of information
    • B01L2300/025—Displaying results or values with integrated means
    • B01L2300/026—Drum counters

Definitions

  • the invention relates to a handheld pipette for the exact repeatable dosing of small and very small liquid volumes with the possibility of changing the volumes of the measured doses in a fluid manner.
  • the manually operated mechanical dosing devices depending on the size of the measured doses, in macropipettes with a volume of 1 ml upwards, up to a maximum of 20 - 30 ml and in micropipettes with a volume of 1 ml downwards up to the order of 2 or even divide by 1 microliter.
  • the mechanical pipettes are used in dosing devices with an unchangeable volume, dosing devices with abruptly selected, unchangeable volumes and dosing devices with the volume that changes in a certain range.
  • the present invention relates to the group of micropipettes with the volume which changes fluently in a certain range.
  • the group of micropipettes irrespective of whether their volume is constant or variable, is also characterized by certain, generally accepted working cycles which follow the suction of the measured amount of liquid into the end piece.
  • the first cycle consists in emptying the aspirated dose from the end piece, the second in the so-called blowing out i.e. Ventilate to remove any residue of the measured amount of liquid and finally the third cycle in the discharge of the one-time, replaceable end piece used for dosing. It was assumed that the cycles mentioned take place in this order in one go under the pressure of the thumb, the beginning of the second and third cycles being signaled by the emergence of an abrupt force threshold, after the overcoming of which only the execution of the given one Cycle occurs.
  • the working cycles, as well as the changes in the dosing volume and the dose size form the generally recognized determinants in the development of micropipette designs.
  • the piston rod On the flat surface of the letter D there is a linear single scale, referring to the upper edge of the body.
  • the piston rod has a screw-shaped section on which there is a screw nut with an external oblong multi-groove profile.
  • This screw nut is inserted inside a rotatable sleeve, which has a toothed wheel on the outside, which with someone else. has the function of an adjusting knob and is coupled with a decimal gear wheel.
  • the inside of the sleeve has a multi-groove profile corresponding to the multi-groove profile on the screw nut of the piston rod over a certain length and is closed from below by a plate under which a spiral pressure spring is located.
  • the piston rod Below the screw part, the piston rod has a cylindrical shape, a stop flange being formed on the piston rod directly below the screw nut. Spiral return springs of the dosing and blow-out mechanism are inserted below the plate.
  • the end piece ejection mechanism is located parallel to the piston rod axis, but in a separate seat shaped in the handle.
  • This mechanism consists of a rod plunger with a return spring supported on the seat base.
  • the push button is attached to the upper end of the plunger with a rugged upper edge.
  • the lower end of the plunger penetrates the hole in the seat base and is screwed to the flange-shaped board of the ejector sleeve, which is slidably mounted on the cylinder.
  • the volume range of the described micropipette is determined by the maximum length of the plunger stroke. This length is determined by the distance that the stop flange has to travel from the upper locking position to the surface of the plate forming the movable sleeve base.
  • the stroke length is changed by turning the scaled toothed wheel, which causes the rotary movement of the sleeve and the screw nut coupled to it through the multi-groove profile, which is screwed onto the piston rod part with a cut thread.
  • the screw stroke on the The piston rod and the translation of the toothed transmission transmitting the movement from the adjusting knob to the sleeve, as well as the plunger diameter, are selected such that one revolution of the toothed adjusting knob causes the piston rod to be displaced by a scale part or by a full unit and the scale part on the adjusting knob is a fraction of this unit corresponds.
  • the push button on the piston rod is pressed and the piston rod is inserted into the body.
  • This movement causes the plunger in the cylinder to move to the depth measured by the actual height of the flange stop above the bottom of the sleeve.
  • the end piece is immersed in the liquid and the pressure on the push button is released evenly with a controlled movement.
  • the system, and with it the plunger returns upwards, causing the liquid to be sucked into the end piece. This takes until the mechanism picks up the top layer Has reached rest position, which is synonymous with the removal of the measured dose.
  • the pipette is emptied by pressing the push button again, which in the first phase causes the plunger to be moved down by the set stroke and the liquid to be ejected from the end piece.
  • the flange stop hits the plate surface
  • the further pushing in of the piston rod encounters an increased resistance caused by the additional effect of the spring supporting the plate.
  • Overcoming the resistance of this spring causes the plate to tear off the sleeve face and permits an additional stroke of the plunger.
  • This additional stroke represents the discharge stroke of the remains of the liquid from the end piece and is always of a constant height, since the foot of the piston rod push button pushes against the upper surface of the body, which makes further movement of the piston rod impossible. Now the drop cycle can take place.
  • the thumb is removed from the piston rod pushbutton and the pressure is transferred to the adjacent pushbutton on the ejector. Its impressions causes the movement of the plunger downwards, which acts on the flange of the ejector sleeve and pulls it down from the plunger cylinder, the sleeve with its edge throwing off the end piece from the nozzle-shaped cylinder outlet.
  • the length of the plunger stroke in this solution is determined by the height of the lower button surface above the upper body surface.
  • the stroke length is changed by the rotation of the piston rod, which causes the rotation of the stopper plug and a less or more deep insertion of the piston rod into the interior of the sleeve defined in the body and, as a result, a lower or higher position of the push button above the pipette body.
  • the rotation of the piston rod simultaneously causes the rotation of the last plate of the digital counter, the number of which is visible in the window, which is cut out in the body.
  • the number size of the counter expresses the size of the set dose in microliters.
  • the dose is measured by pressing the pushbutton until its lower surface is supported on the upper surface of the body.
  • the described micropipette forms a single cycle device, the construction of which does not provide for the discharge and the endpiece ejection cycle to be carried out.
  • the known pipettes were autonomous devices built with a certain dosing range, within which a fluid volume change is possible. So in order to cover the full range assumed for micropipettes, that is from 1 to 1000 microliters, the user must provide himself with a set of special devices with complementary areas for the full range.
  • the object of the invention is to develop such a pipette construction, the metering block of which would be kinetically independent of the other blocks of the mechanism and that an adjusting block could encompass the entire metering range assumed for micropipettes.
  • the plunger metering block can be divided and the division is carried out in such a way that the adjustment block is built into the pipette handle, which consists of a plunger rod pulled through the calibration tube, which is closed with a push button and ends in a stop block , from the forehead a free, thin connector is led out, at the end of which a magnetic coupling is firmly clamped.
  • the calibration screw is in the nut-like bottom with the Push-button coupled and with the forehead in the seat of the immovable body of the digital drum counter, screwed, rotatable switching sleeve, whereby in the handle below the counter there is the blow-out block, which contains a magnetic plate, which is displaceable in the seat flange, due to the section-wise reduction of the inside diameter of the handle, received guidance, arranged spacer sleeve is attached.
  • the second part of the dosing block consists of interchangeable dosing shafts that are screwed into the handle.
  • the dosing shafts contain roller-shaped plungers with different diameters, which are closed off by plate-shaped anchors made of ferromagnetic material and central locking bolts projecting beyond these anchors made of non-magnetic material.
  • Each of these metering shafts consists of a cylinder with a flange, in which a thread is cut on the outside which corresponds to the thread in the handle, a tube provided with a nozzle being pressed into the bottom of this cylinder, which tube is pushed along the entire length by a a filler with a channel is taped and onto this tube an ejector sleeve coupled to a pin plunger with a ring which is inserted within a cup-shaped sleeve arranged above the cylinder is slidably pushed on.
  • reducing sleeves are tightly inserted in the cylinders in the metering shafts equipped with plungers with smaller diameters than the plunger for the area of maximum volume.
  • a sliding cover frame is attached, the arm of which rests on the outer flange of the cup-shaped sleeve.
  • a symbol is arbitrarily formed which symbolizes the limit of the decimal fraction.
  • the outer flange of the cup-shaped sleeve has a different height in each of the exchangeable metering shafts.
  • the switching sleeve is connected to the mechanism of the drum counter by means of the separably inserted connecting sleeve, however after the setting position has been compensated for with the counter zero blocked in the screw-shaped bottom of the sleeve.
  • the gearshift sleeve also has an inner, in an outer wall of the push-button type oblong groove inserted wing-like board.
  • the forehead of the gearshift sleeve is rotatably inserted in the seat formed in the upper part of the drum counter body, on the other hand the bottom of this seat is designed as a horizontal toothed disk which cooperates with the index attached in the gearshift sleeve from the forehead.
  • the entrance to the channel in the dosing shaft is made in the side wall of the conical outlet of the filler.
  • the solution according to the invention is characterized by an advantageous kinetic arrangement, since the piston metering system is a completely independent system which is not burdened by the transmission of any additional forces.
  • the remaining function blocks are installed concentrically around the dosing system, so that the force that actuates them does not act on this system.
  • the separation of the block calibrating the plunger stroke from the reading block allows these two blocks to be easily assembled and their zeros to be precisely synchronized. This increases the accuracy of setting and dimensioning the cans.
  • FIG. 1 shows the pipette in longitudinal section
  • FIGS. 2 and 3 also in longitudinal section two exemplary designs of interchangeable metering stems for two different areas of the can volumes.
  • the pipette according to the invention basically consists of the handle, in the interior of which the following components are permanently installed: block for setting the metering volume, block for reading the set volume and the blow-out block, as well as the metering stems connected to the handle and adjustable to different areas.
  • the dosing shaft set specified in the exemplary embodiment is based on three volume ranges: 2-10 microliters, 10-100 microliters and 100-1000 microliters with the possibility of choosing any volume in each of the specified ranges.
  • the adjusting block used in the handle 1 contains an axially attached piston rod 2, which runs out into a hollow pressure cup 3 protruding beyond the handle 1.
  • the lower end of the piston rod 2 is terminated with the stop block 4, the initial roller shape of which has been somewhat sheared off along the side by a parallel to form guide surface extending to the axis.
  • the calibration screw 7 with a precisely executed thread is slidably inserted, which piston rod is sheared outside for a certain length, this shearing being in one plane with the shearing on the stop stone 4.
  • the calibration screw 1 is screwed into the nut-forming bottom of the selector sleeve 8 rotatably inserted in the handle 1, but through a plate-shaped contact piece 9 with a spring.
  • the inner diameter of the switching sleeve 8 is larger than the diameter of the push button 2, which freely axially into the interior of the sleeve 8 can be introduced.
  • the sleeve 8 also has an inner, oblong, wing-shaped board, which is inserted into an oblong groove 11 made in the outer wall of the pushbutton 3 over its entire length.
  • a spiral return spring 12 is inserted between the bottom of the push button 3 and the bottom of the seat in the calibration screw 3 on the piston rod 2.
  • the setting block is supplemented by the vacuum return damping system arranged inside the hollow pushbutton 3.
  • This system is in the form of a cylinder 13 designed through the interior of the pushbutton 3 and has an elastic piston 14 which is fixed immovably on the upper end of the calibration screw 7 relative to the cylinder 13.
  • the reading block of the set volume is located below the setting block in the handle 1, the main component of which consists of a decimal drum counter 16 arranged near the wall of the handle 1 in the light of the window 17 arranged in the wall.
  • the counter 16 is installed in the body 18 immovably inserted within the handle 1 on a flat ferromagnetic plate 19.
  • the body 18 with a complicated shape has several functions.
  • the bottom of the seat is in the form of a horizontal toothed disk, which cooperates with the index 20 attached from the forehead to the indexing sleeve 8, thereby ensuring that the mechanism of the adjusting block is clearly synchronized with the counter 16.
  • an axial flow bore 21 with a flattened wall which bore has the function of guiding the stop stone 4 and the calibration screw 1, which does not permit any movement of these components.
  • an intermediate gear 22 driving the drum counter 16 is rotatably mounted below the seat, on the cylindrical board formed in the body 18 around the bore 21, an intermediate gear 22 driving the drum counter 16 is rotatably mounted below the seat, on the cylindrical board formed in the body 18 around the bore 21, an intermediate gear 22 driving the drum counter 16 is rotatably mounted. The transfer of the rotary movement of the switching sleeve 8 to the counter 16 takes place with the aid of the connecting sleeve 23, which is used separately, but in a corresponding one Position blocked in the screw-shaped bottom of the shift sleeve 8 and the intermediate gear 22 is coupled to the counter 16.
  • the reading block is also equipped with a sliding cover frame 24, which is attached between the inner wall of the handle 1 and the foreheads of the drums of the counter 16 in the light of the window 17.
  • a sign in the form of, for example, a vertical line, comma or dot which arbitrarily symbolizes the limit of the decimal fraction.
  • the blow-out block is attached in handle 1 below the reading block and concentrically with the plunger of the metering shaft.
  • This block has a flat magnetic plate 25 with a hole through which a free, thin connecting piece 5 passes loosely.
  • the magnetic plate 25 on a ferromagnetic washer is pressed into the front nest in the spacer sleeve 26.
  • the axial guidance of the spacer sleeve 26 was achieved by reducing the inside diameter of the handle 1 in sections.
  • the spiral return spring 27 of the blow-out block is located between the peripheral threshold formed in this way and the flange of the sleeve 26.
  • the interchangeable metering shafts are shown in the exemplary embodiments in FIGS. 2 and 3.
  • Each shaft consists of a cylinder 28 with an externally threaded flange, the diameter of which corresponds to the internal thread in the outlet of the handle 1.
  • a tube 21 is pressed into the bottom of the cylinder 28 and ends in a nozzle 30 placed thereon.
  • a filler 31 with a conical outlet is tightly inserted into the tube 29 along its entire length.
  • the filler 31 has a channel 32 along its entire length, the entrance of which is made in the side wall of the conical outlet of the filler 31.
  • a typical, interchangeable end piece, which was not shown in the drawing, is placed on the end of the connection piece 30.
  • the plunger 33 which is sealed with a circumferential seal 34, cooperates with the cylinder 28.
  • the plunger 33 has the shape of a roller and is closed with a plate-shaped armature 35 made of ferromagnetic material and a central locking bolt 36 projecting beyond this armature made of non-magnetic material.
  • the principle of the unchangeable maximum stroke of the dosing block was adopted in the pipette according to the invention, which is revealed in the maintenance of a calibration screw and a cylinder diameter. However, in order to ensure the possibility of changing the can volume in the entire area for micropipettes, the maximum volume of the metering block for the given sub-area must be changed accordingly.
  • the diameters of the plungers 33 are changed in individual dosing shafts, and by maintaining the constant maximum inner diameter of the cylinder 28, a reduction sleeve 37 with an inner diameter corresponding to the diameter of the plunger 33 for the given sub-range of the dosing volume is pressed into the same .
  • the dosing shaft is also equipped with an end piece ejection block.
  • This block consists of a cup-shaped sleeve 38 arranged above the cylinder 28, within which a ring 39 is movably inserted, via the pin plunger 40, onto the ejector sleeve 41, which comprises the cylinder 28 and is guided on the tube 29.
  • a spiral return spring 42 of the ejector is attached within the ejector sleeve 41 in the area of the tube 29.
  • the Pot-shaped sleeve 38 has an outer flange 12 which controls the displacement of the cover frame 24 which sets the decimal sign between the individual drums thereof in the field of view of the counter 16.
  • the height h of the flange is variable and adapted in such a way that when the dosing shaft is replaced by a shaft with a different volume range, the cover frame 24 is automatically shifted according to the actual need to set the decimal point .
  • the set cover frame 24 will give the reading image of 10.00 and if it will be a dosing shaft with a maximum volume of 100 microliters, the image will accordingly have 100.0.
  • the decimal point is moved outside the field of view.
  • the desired can volume is set by turning the pushbutton 3, which transfers the rotary movement to the switching sleeve 8 by means of the coupling of the groove 11 with the wing-shaped board 10.
  • the rotation of the sleeve 8 causes it to be screwed into the screw-shaped bottom of the calibration screw 7, on the end of which the stop block 4 is pressed.
  • the change in the height at which the forehead of the calibration screw 7 is located also calls for a change in the height of the locking position of the the return screw 12 on the forehead of the calibration screw 1 pressed out stop stone 4.
  • the rotational movement of the switching sleeve 8 is simultaneously transferred through the connecting sleeve 23 rotated together with the sleeve 8 to the intermediate gear 22 and further to the drum counter 16, which numerically represents the height of the face of the calibration screw 7 each time in volume units.
  • the pipette is prepared for the withdrawal of the dose.
  • the push button 3 is pressed until the first sensible resistance, which causes the movement of the piston rod 2 down and the displacement of the stop stone 4 until its forehead rests on the surface of the magnetic plate 25.
  • This movement is transferred via the free connector 5 to the plunger 33, which in the cylinder 28 in one of the ways covered by the stop stone 4 and designated by the word "DOZA" / DOSE / analog depth is inserted.
  • the downward movement of the pushbutton 3 also causes the elastic piston 14 to be introduced into the interior of the cylinder 13.
  • the end piece is then immersed in the liquid to be removed and the pressure on the pushbutton is completely released. Under the action of the return spring 12, the mechanism returns to the top position.
  • the return causes the creation of negative pressure in the cylinder 13, which slows down the return of the pushbutton 3, makes this return relatively uniform and thus enables an exact suction into the end piece of the amount of liquid measured by the stroke of the plunger 33.
  • the return spring 12 which is no longer held back by anything, surely strikes the stop stone 4 against the face of the calibration screw 7.
  • the dose is removed from the end piece when the push button 3 is pressed again until the first sensible resistance, that is, until the moment the stop stone 4 is supported on the surface of the magnetic plate 25. That is the duty cycle.
  • a further push of the push button 3 introduces the blowdown cycle, which can be felt by the formation of the sudden force threshold, since the force of the return spring 12 is now the force of the blowout spring 27 and the force of the magnetic field of the ferromagnetic plate 19 on which the body 18 of the Counter 16 rests, adhering magnetic plate 25 is added. Overcoming this sum of forces causes the magnetic plate 25 to be torn off the plate 19 and its movement together with the spacer sleeve downwards until the end of the sleeve 26 abuts the end of the ring 39.
  • This movement designated in the drawing with the word "WYDMUCH" / EXHAUST / arbitrary, also causes the plunger to be moved by an additional stroke and the end pieces to be ventilated.
EP84114018A 1984-11-20 1984-11-20 Pipette à dose réglable Withdrawn EP0181957A1 (fr)

Priority Applications (1)

Application Number Priority Date Filing Date Title
EP84114018A EP0181957A1 (fr) 1984-11-20 1984-11-20 Pipette à dose réglable

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
EP84114018A EP0181957A1 (fr) 1984-11-20 1984-11-20 Pipette à dose réglable

Publications (1)

Publication Number Publication Date
EP0181957A1 true EP0181957A1 (fr) 1986-05-28

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Application Number Title Priority Date Filing Date
EP84114018A Withdrawn EP0181957A1 (fr) 1984-11-20 1984-11-20 Pipette à dose réglable

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EP (1) EP0181957A1 (fr)

Cited By (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0348612A2 (fr) * 1988-04-15 1990-01-03 Przedsiebiorstwo Polonijno Zagraniczne Plastomed Pipette
EP0364621A1 (fr) * 1988-10-21 1990-04-25 Eppendorf-Netheler-Hinz Gmbh Dispositif pour pipetter
EP0787040A2 (fr) * 1995-07-14 1997-08-06 Rainin Instrument Co.,Inc. Pipette manuelle pourvue d'une assistance par aimant
DE19708151A1 (de) * 1997-02-28 1998-09-10 Eppendorf Geraetebau Netheler Pipettiervorrichtung
DE19826065A1 (de) * 1998-06-12 1999-12-16 Eppendorf Geraetebau Netheler Pipettiervorrichtung
DE10244960A1 (de) * 2002-09-26 2004-04-08 Siemens Ag Kartusche mit Verbindung für einen Pump-Antrieb und Handhabungs-System
DE10237770B4 (de) * 2002-08-17 2006-11-09 Eppendorf Ag Pipettiervorrichtung
EP1875966A1 (fr) * 2006-07-07 2008-01-09 Eppendorf Ag Dispositif de pipetage
DE102012002169A1 (de) * 2012-02-07 2013-08-08 Eppendorf Ag Pipette
EP2732877A1 (fr) * 2012-11-19 2014-05-21 Brand Gmbh + Co Kg Pipette à pistons élévateurs dotés d'une unité de refoulement interchangeable
CN110694706A (zh) * 2019-10-23 2020-01-17 临沂市人民医院 一种多功能移液枪
CN112387321A (zh) * 2019-08-15 2021-02-23 埃佩多夫股份公司 具有可调的计量体积的移液器
CN114505108A (zh) * 2022-03-01 2022-05-17 上海科进生物技术有限公司 一种移液装置及调节方法

Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2330456A1 (fr) * 1975-11-05 1977-06-03 Eppendorf Geraetebau Netheler Dispositif de pipetage
CH624312A5 (en) * 1977-11-04 1981-07-31 Pharmed Sa Micropipette with ejectable tip
DE3137423A1 (de) * 1981-09-19 1983-03-31 Rudolf Brand GmbH & Co, 6980 Wertheim "mikroliterpipette"

Patent Citations (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2330456A1 (fr) * 1975-11-05 1977-06-03 Eppendorf Geraetebau Netheler Dispositif de pipetage
CH624312A5 (en) * 1977-11-04 1981-07-31 Pharmed Sa Micropipette with ejectable tip
DE3137423A1 (de) * 1981-09-19 1983-03-31 Rudolf Brand GmbH & Co, 6980 Wertheim "mikroliterpipette"

Cited By (31)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0348612A3 (fr) * 1988-04-15 1990-09-12 Przedsiebiorstwo Polonijno Zagraniczne Plastomed Pipette
EP0348612A2 (fr) * 1988-04-15 1990-01-03 Przedsiebiorstwo Polonijno Zagraniczne Plastomed Pipette
EP0364621A1 (fr) * 1988-10-21 1990-04-25 Eppendorf-Netheler-Hinz Gmbh Dispositif pour pipetter
US5012682A (en) * 1988-10-21 1991-05-07 Firma Eppendorf-Netheler-Hinz Gmbh Pipetting device
EP0787040A2 (fr) * 1995-07-14 1997-08-06 Rainin Instrument Co.,Inc. Pipette manuelle pourvue d'une assistance par aimant
US5700959A (en) * 1995-07-14 1997-12-23 Rainin Instrument Co., Inc. Manual pipette with magnet assist
EP0787040A4 (fr) * 1995-07-14 1999-05-06 Rainin Instr Co Inc Pipette manuelle pourvue d'une assistance par aimant
DE19708151A1 (de) * 1997-02-28 1998-09-10 Eppendorf Geraetebau Netheler Pipettiervorrichtung
DE19708151C2 (de) * 1997-02-28 1999-05-27 Eppendorf Geraetebau Netheler Pipettiervorrichtung
DE19826065A1 (de) * 1998-06-12 1999-12-16 Eppendorf Geraetebau Netheler Pipettiervorrichtung
DE19826065C2 (de) * 1998-06-12 2000-05-18 Eppendorf Geraetebau Netheler Pipettiervorrichtung
DE10237770B4 (de) * 2002-08-17 2006-11-09 Eppendorf Ag Pipettiervorrichtung
US7182228B2 (en) 2002-09-26 2007-02-27 Siemens Aktiengesellschaft Cartridge with connection for a pump-drive and fluid handling system
DE10244960A1 (de) * 2002-09-26 2004-04-08 Siemens Ag Kartusche mit Verbindung für einen Pump-Antrieb und Handhabungs-System
DE10244960B4 (de) * 2002-09-26 2004-12-02 Siemens Ag Kartusche mit Verbindung für einen Pump-Antrieb und Handhabungs-System für einen flüssigen oder gasförmigen Stoff
EP1875966A1 (fr) * 2006-07-07 2008-01-09 Eppendorf Ag Dispositif de pipetage
US7673532B2 (en) 2006-07-07 2010-03-09 Eppendael AG Pipetting device
CN103447104B (zh) * 2012-02-07 2016-05-18 埃佩多夫股份公司 移液器
DE102012002169A1 (de) * 2012-02-07 2013-08-08 Eppendorf Ag Pipette
EP2626134A3 (fr) * 2012-02-07 2013-11-06 Eppendorf AG Pipette
CN103447104A (zh) * 2012-02-07 2013-12-18 埃佩多夫股份公司 移液器
DE102012002169B4 (de) * 2012-02-07 2017-02-09 Eppendorf Ag Pipette
US20140137672A1 (en) * 2012-11-19 2014-05-22 Brand Gmbh + Co Kg Piston-operated pipette with interchangeable displacement unit
US9364827B2 (en) 2012-11-19 2016-06-14 Brand Gmbh + Co Kg Piston-operated pipette with interchangeable displacement unit
EP2732877A1 (fr) * 2012-11-19 2014-05-21 Brand Gmbh + Co Kg Pipette à pistons élévateurs dotés d'une unité de refoulement interchangeable
CN112387321A (zh) * 2019-08-15 2021-02-23 埃佩多夫股份公司 具有可调的计量体积的移液器
CN112387321B (zh) * 2019-08-15 2022-05-03 埃佩多夫股份公司 具有可调的计量体积的移液器
US11602744B2 (en) 2019-08-15 2023-03-14 Eppendorf Ag Pipette with adjustable dosing volume
CN110694706A (zh) * 2019-10-23 2020-01-17 临沂市人民医院 一种多功能移液枪
CN110694706B (zh) * 2019-10-23 2021-09-03 临沂市人民医院 一种多功能移液枪
CN114505108A (zh) * 2022-03-01 2022-05-17 上海科进生物技术有限公司 一种移液装置及调节方法

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