EA199900596A1 - Способ получения очищенного димера морфогенетического фактора костной ткани - Google Patents

Способ получения очищенного димера морфогенетического фактора костной ткани

Info

Publication number
EA199900596A1
EA199900596A1 EA199900596A EA199900596A EA199900596A1 EA 199900596 A1 EA199900596 A1 EA 199900596A1 EA 199900596 A EA199900596 A EA 199900596A EA 199900596 A EA199900596 A EA 199900596A EA 199900596 A1 EA199900596 A1 EA 199900596A1
Authority
EA
Eurasian Patent Office
Prior art keywords
dimer
bone tissue
stage
obtaining
monomer
Prior art date
Application number
EA199900596A
Other languages
English (en)
Inventor
Джун Хонда
Хидетоси Андоу
Суньджиро Сугимото
Original Assignee
Хёхст Марион Рассел Лтд.
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=18445882&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=EA199900596(A1) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Хёхст Марион Рассел Лтд. filed Critical Хёхст Марион Рассел Лтд.
Publication of EA199900596A1 publication Critical patent/EA199900596A1/ru

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Toxicology (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Описывается способ получения очищенного димерного морфогенетического фактора костной ткани, включающий последовательную обработку тельца включения морфогенетического фактора костной ткани, полученного методами генной инженерии, на перечисленных далее стадиях а-д:а) стадия обработки тельца включения из морфогенетического фактора костной ткани агентом, разворачивающим структуру белка, для получения растворенного мономера;б) стадия обработки растворенного мономера раствором для рефолдинга для получения димера;в) стадия обработки прошедшего рефолдинг димера посредством ультрафильтрации и замещение растворителя;г) стадия изоэлектрического осаждения полученного выше раствора димера; ид) стадия хроматографии с обращенной фазой осажденного в изоэлектрических условиях димера.Международная заявка была опубликована вместе с отчетом о международном поиске.
EA199900596A 1996-12-25 1997-12-24 Способ получения очищенного димера морфогенетического фактора костной ткани EA199900596A1 (ru)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP35581296 1996-12-25
PCT/JP1997/004784 WO1998029559A1 (fr) 1996-12-25 1997-12-24 Procede de preparation de dimeres purifies a partir de facteur derive de l'os

Publications (1)

Publication Number Publication Date
EA199900596A1 true EA199900596A1 (ru) 2000-02-28

Family

ID=18445882

Family Applications (1)

Application Number Title Priority Date Filing Date
EA199900596A EA199900596A1 (ru) 1996-12-25 1997-12-24 Способ получения очищенного димера морфогенетического фактора костной ткани

Country Status (21)

Country Link
US (1) US6551801B1 (ru)
EP (1) EP0949335B2 (ru)
KR (1) KR20000062328A (ru)
AP (1) AP9901539A0 (ru)
AT (1) ATE266095T1 (ru)
AU (1) AU5339598A (ru)
BR (1) BR9714183A (ru)
CA (1) CA2275464A1 (ru)
DE (1) DE69728997T3 (ru)
DK (1) DK0949335T4 (ru)
EA (1) EA199900596A1 (ru)
ES (1) ES2216181T5 (ru)
HU (1) HUP0002573A2 (ru)
IL (1) IL130641A0 (ru)
NO (1) NO993151L (ru)
PL (1) PL334303A1 (ru)
PT (1) PT949335E (ru)
SI (1) SI0949335T2 (ru)
TR (1) TR199901479T2 (ru)
WO (1) WO1998029559A1 (ru)
ZA (1) ZA9711580B (ru)

Families Citing this family (16)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7811793B2 (en) * 1996-12-25 2010-10-12 Biopharma Gesellschaft Zur Biotechnologischen Entwicklung Von Pharmaka Mbh Process for preparing purified active monomer of bone-derived factor
US20040019185A1 (en) * 1996-12-25 2004-01-29 Hidetoshi Andou Process for preparing purified active monomer of bone-derived factor
US20030120042A1 (en) * 2000-03-30 2003-06-26 Takao Yamada Process for producing recombinant protein
AU2003209071B2 (en) * 2002-02-08 2008-08-14 The University Of Erlangen-Nuremberg Formulation comprising bioactive agents and method of using same
CN1735426A (zh) * 2002-08-13 2006-02-15 惠氏公司 用作转化生长因子β蛋白质的溶解赋形剂的肽
JP4800739B2 (ja) * 2005-10-20 2011-10-26 デンカ生研株式会社 アッセイ用媒体及びアッセイ方法
ES2764222T3 (es) * 2006-09-08 2020-06-02 Wyeth Llc Lavado con arginina en la purificación de proteínas mediante el uso de cromatografía de afinidad
AU2007234612B2 (en) 2006-12-14 2013-06-27 Johnson & Johnson Regenerative Therapeutics, Llc Protein stabilization formulations
US7678764B2 (en) 2007-06-29 2010-03-16 Johnson & Johnson Regenerative Therapeutics, Llc Protein formulations for use at elevated temperatures
EP2187932B1 (en) 2007-08-07 2015-01-28 DePuy Synthes Products, LLC Protein formulations comprising gdf-5 in aqueous acidic solution
CN102026619A (zh) * 2008-04-14 2011-04-20 先进科技及再生医学有限责任公司 液体缓冲的gdf-5制剂
US8398611B2 (en) 2010-12-28 2013-03-19 Depuy Mitek, Inc. Compositions and methods for treating joints
US8524662B2 (en) 2010-12-28 2013-09-03 Depuy Mitek, Llc Compositions and methods for treating joints
US8455436B2 (en) 2010-12-28 2013-06-04 Depuy Mitek, Llc Compositions and methods for treating joints
US8623839B2 (en) 2011-06-30 2014-01-07 Depuy Mitek, Llc Compositions and methods for stabilized polysaccharide formulations
US9682099B2 (en) 2015-01-20 2017-06-20 DePuy Synthes Products, Inc. Compositions and methods for treating joints

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA1338663C (en) * 1988-04-08 1996-10-22 Hermann Oppermann Biosynthetic osteogenic proteins and osteogenic devices containing them
CA2141555C (en) * 1992-07-31 2007-03-27 William K. Jones Morphogenic protein soluble complex and composition thereof
JP3570558B2 (ja) * 1993-05-01 2004-09-29 持田製薬株式会社 Ｄｎａ断片およびそれを含むベクター、該ベクターによって形質転換された形質転換体、該ベクターを用いる蛋白質の産生方法
US5385887A (en) * 1993-09-10 1995-01-31 Genetics Institute, Inc. Formulations for delivery of osteogenic proteins
US5399677A (en) * 1993-12-07 1995-03-21 Genetics Institute, Inc. Mutants of bone morphogenetic proteins
BR9608019A (pt) * 1995-04-19 1999-11-30 Hoechst Japan Uma nova proteína e um processo para a preparação da mesma'
US5702637A (en) 1995-04-19 1997-12-30 Minnesota Mining And Manufacturing Company Liquid crystal compounds having a chiral fluorinated terminal portion

Also Published As

Publication number Publication date
HUP0002573A2 (hu) 2000-12-28
KR20000062328A (ko) 2000-10-25
AU5339598A (en) 1998-07-31
SI0949335T1 (en) 2004-10-31
ES2216181T3 (es) 2004-10-16
EP0949335A1 (en) 1999-10-13
TR199901479T2 (xx) 1999-09-21
ATE266095T1 (de) 2004-05-15
NO993151D0 (no) 1999-06-24
ES2216181T5 (es) 2009-03-01
PT949335E (pt) 2004-09-30
DE69728997D1 (de) 2004-06-09
DE69728997T3 (de) 2009-07-09
SI0949335T2 (sl) 2009-04-30
NO993151L (no) 1999-06-24
ZA9711580B (en) 1999-09-23
EP0949335B2 (en) 2008-11-19
US6551801B1 (en) 2003-04-22
DE69728997T2 (de) 2004-09-02
EP0949335A4 (en) 2003-01-22
IL130641A0 (en) 2000-06-01
DK0949335T3 (da) 2004-08-30
DK0949335T4 (da) 2009-03-23
EP0949335B1 (en) 2004-05-06
WO1998029559A1 (fr) 1998-07-09
BR9714183A (pt) 2000-02-29
CA2275464A1 (en) 1998-07-09
PL334303A1 (en) 2000-02-14
AP9901539A0 (en) 1999-06-30

Similar Documents

Publication Publication Date Title
EA199900596A1 (ru) Способ получения очищенного димера морфогенетического фактора костной ткани
EA199700329A1 (ru) Новый белок и способ его получения
DE69029657D1 (de) Menschliches cytokin, interleukin-9
EA200000601A1 (ru) Сайт - специфичное получение конъюгатов grf с полиэтиленгликолем
DK0429570T3 (da) Osteoinducerende præparater
DK0397420T3 (da) Enzymatisk fjernelse af en aminoterminal proteinsekvens
RU93054168A (ru) Димер молекулярного варианта аро-липопротеина, способ его получения, фармацевтическая композиция на его основе
EP0220958A2 (en) Selective chemical removal of a protein amino-terminal residue
DK1364656T3 (da) Brug af knoglemorfogenetiske proteiner til heling og reparation af bindevævsvedhæftning
RU94016354A (ru) Новые полипептидные соединения, способ их получения, фармацевтическая композиция, способ лечения
CY1107514T1 (el) Μια μεθοδος για την εκχυλιση, καθαρισμο και ενζυματικη τροποποιηση της α’ υπομοναδας σογια 7s σφαιρινης για να χρησιμοποιηθει ως παραγων που προκαλει υποχολεστepολαιμια
FR2354340A1 (fr) Procede pour la recuperation de l'antithrombine iii
KR880000465A (ko) 순수한 알부민의 제조방법
DK573587D0 (da) Fremgangsmaade til fremstilling af en faktor viii (ahf)-holdig fraktion
ES2129426T3 (es) Polipeptidos antitrombinicos.
FI103577B1 (fi) Menetelmä, DNA ja mikro-organismi trombolyyttisen aineen valmistamiseksi
ES2053537T3 (es) Aislamiento de la hormona de crecimiento biologicamente activa, monomera.
DE69406214D1 (de) Verfahren zur Reinigung von für den menschlichen Verbrauch bestimmtem Wasser
EA200000406A1 (ru) Способ получения замещенных 4-фенил-4-цианоциклогексановых кислот
EP0241136A3 (en) Human class 1 heparin-binding growth factor
KR960700271A (ko) 인간 호흡계 합포체 바이러스 fg 당단백질의 정제 및 리폴딩 방법(process for the purification and refolding of human respiratory syncytial virus fg glycoprotein)
IT1163772B (it) Oligosaccaridi eterogenei complessabili ed alfa-idosani ad attivita' terapeutica, procedimento per la loro preparazione e relative composizioni farmaceutiche
SU1127525A3 (ru) Способ выделени альбумина
KR950003312A (ko) 연골세포 단백질
US7811793B2 (en) Process for preparing purified active monomer of bone-derived factor