EA034039B1 - Клетка-хозяин, способная к сайт-специфической интеграции, способ ее получения и применение - Google Patents
Клетка-хозяин, способная к сайт-специфической интеграции, способ ее получения и применение Download PDFInfo
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- EA034039B1 EA034039B1 EA201590068A EA201590068A EA034039B1 EA 034039 B1 EA034039 B1 EA 034039B1 EA 201590068 A EA201590068 A EA 201590068A EA 201590068 A EA201590068 A EA 201590068A EA 034039 B1 EA034039 B1 EA 034039B1
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- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
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- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
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- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
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| PCT/EP2013/062859 WO2013190032A1 (en) | 2012-06-22 | 2013-06-20 | Site-specific integration |
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| EA201590068A1 EA201590068A1 (ru) | 2015-04-30 |
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| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| CN113981027A (zh) * | 2015-04-27 | 2022-01-28 | 动量制药公司 | 制备治疗性蛋白质的方法 |
| US11261462B2 (en) | 2016-01-27 | 2022-03-01 | Just-Evotec Biologics, Inc. | Inducible expression from transposon-based vectors and uses |
| US11098310B2 (en) | 2016-01-27 | 2021-08-24 | Just-Evotec Biologics, Inc. | Expression from transposon-based vectors and uses |
| EP3408397B1 (en) | 2016-01-27 | 2020-07-15 | Just-Evotec Biologics, Inc. | Hybrid promoter and uses thereof |
| EP3445780A1 (en) * | 2016-04-20 | 2019-02-27 | Regeneron Pharmaceuticals, Inc. | Compositions and methods for making antibodies based on use of an expression-enhancing locus |
| TWI827531B (zh) * | 2016-04-20 | 2024-01-01 | 美商再生元醫藥公司 | 基於使用增強表現基因座之製備抗體的組成物及方法 |
| CA3053712A1 (en) * | 2017-02-17 | 2018-08-23 | Lonza Ltd. | Multi-site specific integration cells for difficult to express proteins |
| CA3053304A1 (en) * | 2017-02-17 | 2018-08-23 | Lonza Ltd. | Mammalian cells for producing adeno-associated viruses |
| GB201703418D0 (en) * | 2017-03-03 | 2017-04-19 | Ge Healthcare Bio Sciences Ab | Method for cell line development |
| CA3056182A1 (en) | 2017-03-16 | 2018-09-20 | Pfizer Inc. | Tyrosine prototrophy |
| CN107557390A (zh) * | 2017-09-18 | 2018-01-09 | 江南大学 | 一种筛选cho细胞系高表达位点的方法 |
| IL275462B1 (en) * | 2017-12-22 | 2025-09-01 | Genentech Inc | Targeted integration of nucleic acids |
| JP7549582B2 (ja) * | 2018-10-01 | 2024-09-11 | ロンザ リミテッド | 予測可能かつ安定な導入遺伝子発現を有するｓｓｉ細胞および形成の方法 |
| AU2019403279A1 (en) * | 2018-12-21 | 2021-07-01 | Genentech, Inc. | Targeted integration of nucleic acids |
| EP3950938A4 (en) * | 2019-04-02 | 2023-01-18 | Chugai Seiyaku Kabushiki Kaisha | METHOD FOR INTRODUCING A FOREIGN TARGET-SPECIFIC GENE |
| KR20220097910A (ko) | 2019-11-14 | 2022-07-08 | 론자 리미티드 | 세포 선택 방법 |
| KR102335242B1 (ko) | 2020-05-22 | 2021-12-02 | 인천대학교 산학협력단 | Fer1L4 유전자에 부위-특이적 통합된 RMCE 랜딩 패드를 포함하는 CHO 세포 |
| US20230287455A1 (en) | 2020-07-30 | 2023-09-14 | Pfizer Inc. | Cells Having Gene Duplications and Uses Thereof |
| JP2024528139A (ja) | 2021-08-02 | 2024-07-26 | ファイザー・インク | 改良された発現ベクターおよびその使用 |
| AU2022373653A1 (en) | 2021-10-18 | 2024-05-02 | Regeneron Pharmaceuticals, Inc. | Eukaryotic cells comprising adenovirus-associated virus polynucleotides |
| WO2023148598A1 (en) | 2022-02-02 | 2023-08-10 | Pfizer Inc. | Cysteine prototrophy |
| CN119955730A (zh) * | 2023-11-08 | 2025-05-09 | 深圳太力生物技术有限责任公司 | 一种细胞株及其制备方法与应用 |
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| FR2646438B1 (fr) | 1989-03-20 | 2007-11-02 | Pasteur Institut | Procede de remplacement specifique d'une copie d'un gene present dans le genome receveur par l'integration d'un gene different de celui ou se fait l'integration |
| DE69840439D1 (de) | 1997-04-24 | 2009-02-26 | Univ Washington | Zielgerichtete genveraenderung mit parvoviralen vektoren |
| BRPI0513706A (pt) * | 2004-07-20 | 2008-05-13 | Symphogen As | anticorpo policlonal recombinante anti-rhesus d e métodos de produção |
| JP4665115B2 (ja) * | 2004-08-06 | 2011-04-06 | 独立行政法人理化学研究所 | 骨・関節疾患感受性遺伝子およびその用途 |
| WO2007014275A2 (en) | 2005-07-26 | 2007-02-01 | Sangamo Biosciences, Inc. | Targeted integration and expression of exogenous nucleic acid sequences |
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2012
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| ANDERSEN VIBEKE; ERNST ANJA; SVENTORAITYTE JURGITA; KUPCINSKAS LIMAS; JACOBSEN BENT A; KRARUP HENRIK B; VOGEL ULLA; JONAITIS LAIMA: "Assessment of heterogeneity between European Populations: a Baltic and Danish replication case-control study of SNPs from a recent European ulcerative colitis genome wide association study", BMC MEDICAL GENETICS, BIOMED CENTRAL, LONDON, GB, vol. 12, no. Article 139, 1 October 2011 (2011-10-01), GB, pages 9 pp., XP009165387, ISSN: 1471-2350, DOI: 10.1186/1471-2350-12-139 * |
| C. ZHANG, N.G. CORTEZ, AND K.I. BERNS: "Characterization of a Bipartite Recombinant Adeno-Associated Viral Vector for Site-Specific Integration", HUMAN GENE THERAPY, MARY ANN LIEBERT, INC. PUBLISHERS, GB, vol. 18, no. 9, 1 September 2007 (2007-09-01), GB, pages 787 - 797, XP002688694, ISSN: 1043-0342, DOI: 10.1089/hum.2007.056 * |
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