SG11201408576QA - Site-specific integration - Google Patents
Site-specific integrationInfo
- Publication number
- SG11201408576QA SG11201408576QA SG11201408576QA SG11201408576QA SG11201408576QA SG 11201408576Q A SG11201408576Q A SG 11201408576QA SG 11201408576Q A SG11201408576Q A SG 11201408576QA SG 11201408576Q A SG11201408576Q A SG 11201408576QA SG 11201408576Q A SG11201408576Q A SG 11201408576QA
- Authority
- SG
- Singapore
- Prior art keywords
- site
- specific integration
- integration
- specific
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
- C12N15/90—Stable introduction of foreign DNA into chromosome
- C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
- C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
- C12N15/86—Viral vectors
- C12N15/861—Adenoviral vectors
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
- C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2317/00—Immunoglobulins specific features
- C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
- C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2800/00—Nucleic acids vectors
- C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US201261663147P | 2012-06-22 | 2012-06-22 | |
| EP12185330.3A EP2711428A1 (en) | 2012-09-21 | 2012-09-21 | Site-specific integration |
| PCT/EP2013/062859 WO2013190032A1 (en) | 2012-06-22 | 2013-06-20 | Site-specific integration |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| SG11201408576QA true SG11201408576QA (en) | 2015-03-30 |
Family
ID=46888327
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| SG11201408576QA SG11201408576QA (en) | 2012-06-22 | 2013-06-20 | Site-specific integration |
Country Status (19)
| Country | Link |
|---|---|
| US (2) | US10280436B2 (en) |
| EP (2) | EP2711428A1 (en) |
| JP (1) | JP6053923B2 (en) |
| KR (1) | KR101761709B1 (en) |
| CN (1) | CN104379753B (en) |
| AU (1) | AU2013279333C1 (en) |
| BR (1) | BR112014032299B1 (en) |
| CA (1) | CA2876280C (en) |
| DK (1) | DK2864489T3 (en) |
| EA (1) | EA034039B1 (en) |
| ES (1) | ES2629509T3 (en) |
| HK (1) | HK1202580A1 (en) |
| IL (1) | IL236347A0 (en) |
| IN (1) | IN2014MN02518A (en) |
| MX (1) | MX351727B (en) |
| PL (1) | PL2864489T3 (en) |
| SG (1) | SG11201408576QA (en) |
| TW (1) | TWI523946B (en) |
| WO (1) | WO2013190032A1 (en) |
Families Citing this family (20)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB201407852D0 (en) | 2014-05-02 | 2014-06-18 | Iontas Ltd | Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules |
| EP3289092B1 (en) | 2015-04-27 | 2020-02-12 | Momenta Pharmaceuticals, Inc. | Method of manufacturing a therapeutic protein |
| JP6937309B2 (en) | 2016-01-27 | 2021-09-22 | ジャスト−エヴォテック バイオロジックス、インコーポレイテッド | Hybrid promoter and its use |
| US11261462B2 (en) | 2016-01-27 | 2022-03-01 | Just-Evotec Biologics, Inc. | Inducible expression from transposon-based vectors and uses |
| US11098310B2 (en) | 2016-01-27 | 2021-08-24 | Just-Evotec Biologics, Inc. | Expression from transposon-based vectors and uses |
| SG10202010155YA (en) * | 2016-04-20 | 2020-11-27 | Regeneron Pharma | Compositions and methods for making antibodies based on use of expression-enhancing loci |
| AR108295A1 (en) * | 2016-04-20 | 2018-08-08 | Regeneron Pharma | COMPOSITIONS AND METHODS TO PREPARE ANTIBODIES BASED ON THE USE OF AN EXPRESSION IMPROVEMENT LOCUS |
| JP2020507331A (en) | 2017-02-17 | 2020-03-12 | ロンザ リミテッドＬｏｎｚａ Ｌｉｍｉｔｅｄ | Mammalian cells for producing adeno-associated virus |
| US20200002727A1 (en) * | 2017-02-17 | 2020-01-02 | Lonza Ltd. | Multi-site specific integration cells for difficult to express proteins |
| GB201703418D0 (en) * | 2017-03-03 | 2017-04-19 | Ge Healthcare Bio Sciences Ab | Method for cell line development |
| WO2018167621A1 (en) | 2017-03-16 | 2018-09-20 | Pfizer Inc. | Tyrosine prototrophy |
| CN107557390A (en) * | 2017-09-18 | 2018-01-09 | 江南大学 | A kind of method for screening the high expression sites of Chinese hamster ovary celI system |
| US20220049275A1 (en) * | 2018-10-01 | 2022-02-17 | Lonza, Ltd. | Ssi cells with predictable and stable transgene expression and methods of formation |
| CA3123448A1 (en) * | 2018-12-21 | 2020-06-25 | Genentech, Inc. | Targeted integration of nucleic acids |
| US20220170047A1 (en) | 2019-04-02 | 2022-06-02 | Chugai Seiyaku Kabushiki Kaisha | Method of introducing target-specific foreign gene |
| CN114746554A (en) | 2019-11-14 | 2022-07-12 | 隆萨有限公司 | Method for cell selection |
| KR102335242B1 (en) | 2020-05-22 | 2021-12-02 | 인천대학교 산학협력단 | Cho cell comprising site-specific integrated rmce landing pad in fer1l4 gene |
| US20230287455A1 (en) | 2020-07-30 | 2023-09-14 | Pfizer Inc. | Cells Having Gene Duplications and Uses Thereof |
| CA3227875A1 (en) | 2021-08-02 | 2023-02-09 | Pfizer Inc. | Improved expression vectors and uses thereof |
| WO2023148598A1 (en) | 2022-02-02 | 2023-08-10 | Pfizer Inc. | Cysteine prototrophy |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FR2646438B1 (en) | 1989-03-20 | 2007-11-02 | Pasteur Institut | A METHOD FOR SPECIFIC REPLACEMENT OF A COPY OF A GENE PRESENT IN THE RECEIVER GENOME BY INTEGRATION OF A GENE DIFFERENT FROM THAT OR INTEGRATION |
| DE69840439D1 (en) | 1997-04-24 | 2009-02-26 | Univ Washington | TARGETED GENERIC CHANGE WITH PARVOVIRAL VECTORS |
| EP1967529A1 (en) * | 2004-07-20 | 2008-09-10 | Symphogen A/S | Anti-rhesus D recombinant polyclonal antibody and methods of manufacture |
| JP4665115B2 (en) * | 2004-08-06 | 2011-04-06 | 独立行政法人理化学研究所 | Bone and joint disease susceptibility genes and their uses |
| KR101419729B1 (en) | 2005-07-26 | 2014-07-17 | 상가모 바이오사이언스 인코포레이티드 | Targeted integration and expression of exogenous nucleic acid sequences |
-
2012
- 2012-09-21 EP EP12185330.3A patent/EP2711428A1/en not_active Withdrawn
-
2013
- 2013-06-20 EA EA201590068A patent/EA034039B1/en not_active IP Right Cessation
- 2013-06-20 CN CN201380032837.8A patent/CN104379753B/en active Active
- 2013-06-20 AU AU2013279333A patent/AU2013279333C1/en not_active Ceased
- 2013-06-20 KR KR1020157000511A patent/KR101761709B1/en active IP Right Grant
- 2013-06-20 IN IN2518MUN2014 patent/IN2014MN02518A/en unknown
- 2013-06-20 WO PCT/EP2013/062859 patent/WO2013190032A1/en active Application Filing
- 2013-06-20 ES ES13729962.4T patent/ES2629509T3/en active Active
- 2013-06-20 CA CA2876280A patent/CA2876280C/en active Active
- 2013-06-20 US US14/409,283 patent/US10280436B2/en active Active
- 2013-06-20 SG SG11201408576QA patent/SG11201408576QA/en unknown
- 2013-06-20 EP EP13729962.4A patent/EP2864489B1/en active Active
- 2013-06-20 DK DK13729962.4T patent/DK2864489T3/en active
- 2013-06-20 PL PL13729962T patent/PL2864489T3/en unknown
- 2013-06-20 JP JP2015517758A patent/JP6053923B2/en active Active
- 2013-06-20 MX MX2014015612A patent/MX351727B/en active IP Right Grant
- 2013-06-20 BR BR112014032299-6A patent/BR112014032299B1/en not_active IP Right Cessation
- 2013-06-21 TW TW102122188A patent/TWI523946B/en active
-
2014
- 2014-12-18 IL IL236347A patent/IL236347A0/en active IP Right Grant
-
2015
- 2015-03-26 HK HK15103066.6A patent/HK1202580A1/en unknown
-
2018
- 2018-10-31 US US16/176,525 patent/US11326185B2/en active Active
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