IN2014MN02518A - - Google Patents

Info

Publication number
IN2014MN02518A
IN2014MN02518A IN2518MUN2014A IN2014MN02518A IN 2014MN02518 A IN2014MN02518 A IN 2014MN02518A IN 2518MUN2014 A IN2518MUN2014 A IN 2518MUN2014A IN 2014MN02518 A IN2014MN02518 A IN 2014MN02518A
Authority
IN
India
Prior art keywords
host cells
ssi
cho
stable
produce
Prior art date
Application number
Inventor
James Rance
Robert Young
Michael J Agostino
Mark Moffat
Lin Zhang
Baohong Zhang
Original Assignee
Lonza Biologics Plc
Pfizer
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Lonza Biologics Plc, Pfizer filed Critical Lonza Biologics Plc
Publication of IN2014MN02518A publication Critical patent/IN2014MN02518A/en

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C12N15/902—Stable introduction of foreign DNA into chromosome using homologous recombination
    • C12N15/907—Stable introduction of foreign DNA into chromosome using homologous recombination in mammalian cells
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C12N15/86—Viral vectors
    • C12N15/861—Adenoviral vectors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/87—Introduction of foreign genetic material using processes not otherwise provided for, e.g. co-transformation
    • C12N15/90—Stable introduction of foreign DNA into chromosome
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/10—Immunoglobulins specific features characterized by their source of isolation or production
    • C07K2317/14—Specific host cells or culture conditions, e.g. components, pH or temperature
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2317/00—Immunoglobulins specific features
    • C07K2317/20—Immunoglobulins specific features characterized by taxonomic origin
    • C07K2317/24—Immunoglobulins specific features characterized by taxonomic origin containing regions, domains or residues from different species, e.g. chimeric, humanized or veneered
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/22—Vectors comprising a coding region that has been codon optimised for expression in a respective host
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2800/00—Nucleic acids vectors
    • C12N2800/30—Vector systems comprising sequences for excision in presence of a recombinase, e.g. loxP or FRT

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Mycology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Immunology (AREA)
  • Medicinal Chemistry (AREA)
  • Cell Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

The present invention relates to stable and high producing site specific integration (SSI) host cells e. g. Chinese hamster ovary (CHO) derived host cells methods to produce and to use them.
IN2518MUN2014 2012-06-22 2013-06-20 IN2014MN02518A (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US201261663147P 2012-06-22 2012-06-22
EP12185330.3A EP2711428A1 (en) 2012-09-21 2012-09-21 Site-specific integration
PCT/EP2013/062859 WO2013190032A1 (en) 2012-06-22 2013-06-20 Site-specific integration

Publications (1)

Publication Number Publication Date
IN2014MN02518A true IN2014MN02518A (en) 2015-07-17

Family

ID=46888327

Family Applications (1)

Application Number Title Priority Date Filing Date
IN2518MUN2014 IN2014MN02518A (en) 2012-06-22 2013-06-20

Country Status (19)

Country Link
US (2) US10280436B2 (en)
EP (2) EP2711428A1 (en)
JP (1) JP6053923B2 (en)
KR (1) KR101761709B1 (en)
CN (1) CN104379753B (en)
AU (1) AU2013279333C1 (en)
BR (1) BR112014032299B1 (en)
CA (1) CA2876280C (en)
DK (1) DK2864489T3 (en)
EA (1) EA034039B1 (en)
ES (1) ES2629509T3 (en)
HK (1) HK1202580A1 (en)
IL (1) IL236347A0 (en)
IN (1) IN2014MN02518A (en)
MX (1) MX351727B (en)
PL (1) PL2864489T3 (en)
SG (1) SG11201408576QA (en)
TW (1) TWI523946B (en)
WO (1) WO2013190032A1 (en)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201407852D0 (en) 2014-05-02 2014-06-18 Iontas Ltd Preparation of libraries od protein variants expressed in eukaryotic cells and use for selecting binding molecules
HUE050105T2 (en) * 2015-04-27 2020-11-30 Momenta Pharmaceuticals Inc Method of manufacturing a therapeutic protein
US11261462B2 (en) 2016-01-27 2022-03-01 Just-Evotec Biologics, Inc. Inducible expression from transposon-based vectors and uses
JP6937309B2 (en) 2016-01-27 2021-09-22 ジャスト−エヴォテック バイオロジックス、インコーポレイテッド Hybrid promoter and its use
US11098310B2 (en) 2016-01-27 2021-08-24 Just-Evotec Biologics, Inc. Expression from transposon-based vectors and uses
CN116515757A (en) * 2016-04-20 2023-08-01 瑞泽恩制药公司 Compositions and methods based on the use of expression enhancing loci for the production of antibodies
IL309065A (en) 2016-04-20 2024-02-01 Regeneron Pharma Compositions and methods for making antibodies based on use of an expression-enhancing locus
WO2018150271A1 (en) 2017-02-17 2018-08-23 Lonza Ltd. Mammalian cells for producing adeno-associated viruses
CN111372946A (en) * 2017-02-17 2020-07-03 隆萨有限公司 Multi-site specific integration cells of difficult to express proteins
GB201703418D0 (en) * 2017-03-03 2017-04-19 Ge Healthcare Bio Sciences Ab Method for cell line development
JP7177076B2 (en) 2017-03-16 2022-11-22 ファイザー・インク tyrosine prototrophy
CN107557390A (en) * 2017-09-18 2018-01-09 江南大学 A kind of method for screening the high expression sites of Chinese hamster ovary celI system
SG11202103111TA (en) * 2018-10-01 2021-04-29 Lonza Ag Ssi cells with predictable and stable transgene expression and methods of formation
TW202039852A (en) * 2018-12-21 2020-11-01 美商建南德克公司 Targeted integration of nucleic acids
TW202043478A (en) 2019-04-02 2020-12-01 日商中外製藥股份有限公司 Method of introducing target-specific foreign gene
WO2021094461A1 (en) 2019-11-14 2021-05-20 Lonza Ltd Methods of cell selection
KR102335242B1 (en) * 2020-05-22 2021-12-02 인천대학교 산학협력단 Cho cell comprising site-specific integrated rmce landing pad in fer1l4 gene
JP2023535604A (en) 2020-07-30 2023-08-18 ファイザー・インク Cells with gene duplication and uses thereof
EP4380969A1 (en) 2021-08-02 2024-06-12 Pfizer Inc. Improved expression vectors and uses thereof
WO2023148598A1 (en) 2022-02-02 2023-08-10 Pfizer Inc. Cysteine prototrophy

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2646438B1 (en) 1989-03-20 2007-11-02 Pasteur Institut A METHOD FOR SPECIFIC REPLACEMENT OF A COPY OF A GENE PRESENT IN THE RECEIVER GENOME BY INTEGRATION OF A GENE DIFFERENT FROM THAT OR INTEGRATION
CA2289277C (en) 1997-04-24 2013-02-12 University Of Washington Targeted gene modification by parvoviral vectors
EP1771483A1 (en) * 2004-07-20 2007-04-11 Symphogen A/S Anti-rhesus d recombinant polyclonal antibody and methods of manufacture
JP4665115B2 (en) * 2004-08-06 2011-04-06 独立行政法人理化学研究所 Bone and joint disease susceptibility genes and their uses
CN101273141B (en) 2005-07-26 2013-03-27 桑格摩生物科学股份有限公司 Targeted integration and expression of exogenous nucleic acid sequences

Also Published As

Publication number Publication date
AU2013279333C1 (en) 2017-08-31
US20150167020A1 (en) 2015-06-18
KR101761709B1 (en) 2017-07-26
HK1202580A1 (en) 2015-10-02
CA2876280A1 (en) 2013-12-27
IL236347A0 (en) 2015-02-26
AU2013279333B2 (en) 2017-03-30
JP6053923B2 (en) 2016-12-27
CN104379753A (en) 2015-02-25
AU2013279333B9 (en) 2017-06-29
EP2864489B1 (en) 2017-03-29
JP2015519914A (en) 2015-07-16
MX2014015612A (en) 2015-07-14
US11326185B2 (en) 2022-05-10
US10280436B2 (en) 2019-05-07
US20190119702A1 (en) 2019-04-25
AU2013279333A1 (en) 2015-02-12
EP2711428A1 (en) 2014-03-26
MX351727B (en) 2017-10-26
PL2864489T3 (en) 2017-09-29
KR20150029693A (en) 2015-03-18
BR112014032299A2 (en) 2017-08-01
BR112014032299B1 (en) 2021-09-21
EA201590068A1 (en) 2015-04-30
ES2629509T3 (en) 2017-08-10
CA2876280C (en) 2018-02-06
CN104379753B (en) 2020-06-09
SG11201408576QA (en) 2015-03-30
WO2013190032A1 (en) 2013-12-27
TWI523946B (en) 2016-03-01
EP2864489A1 (en) 2015-04-29
EA034039B1 (en) 2019-12-20
DK2864489T3 (en) 2017-07-17
TW201420762A (en) 2014-06-01

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