DK2336172T3 - Fjernelse af aggregater med høj molvægt ved hydroxylapatit-chromatografi - Google Patents
Fjernelse af aggregater med høj molvægt ved hydroxylapatit-chromatografi Download PDFInfo
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- DK2336172T3 DK2336172T3 DK10174366.4T DK10174366T DK2336172T3 DK 2336172 T3 DK2336172 T3 DK 2336172T3 DK 10174366 T DK10174366 T DK 10174366T DK 2336172 T3 DK2336172 T3 DK 2336172T3
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- Prior art keywords
- antibody
- buffer
- column
- sodium phosphate
- chromatography
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
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- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
- C07K1/14—Extraction; Separation; Purification
- C07K1/16—Extraction; Separation; Purification by chromatography
- C07K1/165—Extraction; Separation; Purification by chromatography mixed-mode chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3847—Multimodal interactions
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/02—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material
- B01J20/04—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium
- B01J20/048—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof comprising inorganic material comprising compounds of alkali metals, alkaline earth metals or magnesium containing phosphorus, e.g. phosphates, apatites, hydroxyapatites
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01J—CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOID CHEMISTRY; THEIR RELEVANT APPARATUS
- B01J20/00—Solid sorbent compositions or filter aid compositions; Sorbents for chromatography; Processes for preparing, regenerating or reactivating thereof
- B01J20/281—Sorbents specially adapted for preparative, analytical or investigative chromatography
- B01J20/282—Porous sorbents
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/06—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies from serum
- C07K16/065—Purification, fragmentation
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/22—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against growth factors ; against growth regulators
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/24—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against cytokines, lymphokines or interferons
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K16/00—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies
- C07K16/18—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans
- C07K16/28—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants
- C07K16/2851—Immunoglobulins [IGs], e.g. monoclonal or polyclonal antibodies against material from animals or humans against receptors, cell surface antigens or cell surface determinants against the lectin superfamily, e.g. CD23, CD72
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/36—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving ionic interaction
- B01D15/361—Ion-exchange
-
- B—PERFORMING OPERATIONS; TRANSPORTING
- B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
- B01D—SEPARATION
- B01D15/00—Separating processes involving the treatment of liquids with solid sorbents; Apparatus therefor
- B01D15/08—Selective adsorption, e.g. chromatography
- B01D15/26—Selective adsorption, e.g. chromatography characterised by the separation mechanism
- B01D15/38—Selective adsorption, e.g. chromatography characterised by the separation mechanism involving specific interaction not covered by one or more of groups B01D15/265 - B01D15/36
- B01D15/3804—Affinity chromatography
- B01D15/3809—Affinity chromatography of the antigen-antibody type, e.g. protein A, G, L chromatography
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- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Health & Medical Sciences (AREA)
- Analytical Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biochemistry (AREA)
- Biophysics (AREA)
- Immunology (AREA)
- Genetics & Genomics (AREA)
- Medicinal Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Chemical Kinetics & Catalysis (AREA)
- Inorganic Chemistry (AREA)
- Peptides Or Proteins (AREA)
- Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Solid-Sorbent Or Filter-Aiding Compositions (AREA)
Claims (17)
1. Fremgangsmåde til oprensning af mindst én antistofmonomer fra et antistofpræparat, der indeholder aggregater med høj molvægt, ved hvilken antistofpræparatet underkastes hydroxylapatit-chromatografi, idet hydroxylapatit-chromatografien omfatter at bringe en hydroxylapatit-harpiks i kontakt med antistofpræparatet i en søjle, idet søjlen er ækvilibreret med en ækvilibreringspuffer, der har en pH-værdi fra 6.4 til 7,4 og omfatter fra 1 til 20 mM natriumphosphat og fra 0 til 200 mM NaCI, og eventuelt at vaske søjlen med en vaskepuffer, der har en pH-værdi fra 6,4 til 7.4 og omfatter fra 1 til 20 mM natriumphosphat og fra 0 til 200 mM NaCI, og at eluere renset antistof fra harpiksen med mindst én elueringspuffer, der har en pH-værdi fra 6,4 til 7,6 og omfatter fra 1 til 20 mM natriumphosphat og fra 0,2 til 2,5 M NaCI.
2. Fremgangsmåde ifølge krav 1, ved hvilken antistofpræparatet endvidere underkastes (a) protein-A-affinitetschromatografi og (b) ionbytterchromatografi.
3. Fremgangsmåde ifølge krav 2, ved hvilken protein-A-affinitetschromatografi udføres først, og hydroxylapatit-chromatografien udføres sidst.
4. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 3, ved hvilken hydroxylapatit-chromatografien omfatter: at bringe en hydroxylapatit-harpiks i kontakt med antistofpræparatet i en søjle, idet søjlen i et første trin er ækvilibreret med en ækvilibreringspuffer, der har en pH-værdi fra 6,4 til 7,4 og omfatter fra 10 til 500 mM natriumphosphat og 1 M NaCI og i et andet trin med en ækvilibreringspuffer, der har en pH-værdi fra 6,4 til 7,4 og omfatter fra 1 til 20 mM natriumphosphat og fra 0 til 200 mM NaCI, og eventuelt at vaske søjlen med en vaskepuffer, der har en pH-værdi fra 6,4 til 7.4 og omfatter fra 1 til 20 mM natriumphosphat og fra 0 til 200 mM NaCI, og at eluere renset antistof fra harpiksen med mindst én elueringspuffer, der har en pH-værdi fra 6,4 til 7,6 og omfatter fra 1 til 20 mM natriumphosphat og fra 0,2 til 2,5 M NaCI.
5. Fremgangsmåde ifølge et hvilket som helst af kravene 1 til 4, ved hvilken hydroxylapatit-chromatografien omfatter det trin at vaske søjlen med en vaskepuffer, der har en pH-værdi fra 6,4 til 7,4 og omfatter fra 1 til 20 mM natriumphosphat og fra 0 til 200 mM NaCI.
6. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, ved hvilken det rensede antistof indeholder mindre end 5% aggregater med høj molvægt.
7. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, ved hvilken elueringspufferen indeholder 3 mM eller 5 mM natriumphosphat.
8. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, ved hvilken elueringspufferen indeholder 0,3 M til 2,5 M NaCI.
9. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, ved hvilken elueringspufferen indeholder 0,3 M til 1,1 M NaCI.
10. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, ved hvilken elueringspufferen indeholder 0,35 M til 1 M NaCI.
11. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, ved hvilken antistoffet er et IgG-, IgA-, IgD-, IgE- eller IgM-antistof.
12. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, ved hvilken antistoffet er monoklont, polyklont, kimært, humaniseret eller et fragment deraf.
13. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, ved hvilken antistoffet er en anti-IL-21-receptor-, anti-GDF-8-, anti-Abeta-, anti-CD22-, anti-Lewis-Y-, anti-IL-13- eller anti-IL-22-antistof.
14. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, ved hvilken antistoffet har en basisk pi.
15. Fremgangsmåde ifølge et hvilket som helst af de foregående krav, ved hvilken harpiksen er keramisk hydroxylapatit type I eller II.
16. Fremgangsmåde ifølge krav 2, ved hvilken ionbytterchromatografien er anionbytterchromatografi.
17. Fremgangsmåde ifølge et hvilket som helst foregående krav, ved hvilken det rensede antistof indeholder mindre 300 ppm protein A.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US51401803P | 2003-10-27 | 2003-10-27 | |
| US52333503P | 2003-11-20 | 2003-11-20 | |
| EP04794288.3A EP1678208B1 (en) | 2003-10-27 | 2004-10-06 | Removal of high molecular weight aggregates using hydroxyapatite chromatography |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK2336172T3 true DK2336172T3 (da) | 2015-01-26 |
Family
ID=34576743
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK10174366.4T DK2336172T3 (da) | 2003-10-27 | 2004-10-06 | Fjernelse af aggregater med høj molvægt ved hydroxylapatit-chromatografi |
| DK04794288.3T DK1678208T3 (da) | 2003-10-27 | 2004-10-06 | Fjernelse af aggregater med høj molekylvægt under anvendelse af hydroxyapatitchromatografi |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK04794288.3T DK1678208T3 (da) | 2003-10-27 | 2004-10-06 | Fjernelse af aggregater med høj molekylvægt under anvendelse af hydroxyapatitchromatografi |
Country Status (19)
| Country | Link |
|---|---|
| US (2) | US9469672B2 (da) |
| EP (2) | EP1678208B1 (da) |
| JP (2) | JP5004586B2 (da) |
| KR (2) | KR101321876B1 (da) |
| CN (2) | CN102276717B (da) |
| AU (1) | AU2004286938B2 (da) |
| BR (2) | BR122020007658B1 (da) |
| CA (1) | CA2543193C (da) |
| CO (1) | CO5690649A2 (da) |
| DK (2) | DK2336172T3 (da) |
| EC (1) | ECSP066583A (da) |
| ES (2) | ES2418830T3 (da) |
| IL (1) | IL175229A (da) |
| MX (1) | MXPA06004472A (da) |
| NZ (1) | NZ547315A (da) |
| PL (2) | PL1678208T3 (da) |
| PT (2) | PT2336172E (da) |
| RU (2) | RU2409591C2 (da) |
| WO (1) | WO2005044856A2 (da) |
Families Citing this family (70)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0304576D0 (en) * | 2003-02-28 | 2003-04-02 | Lonza Biologics Plc | Protein a chromatography |
| CA2543193C (en) * | 2003-10-27 | 2015-08-11 | Wyeth | Removal of high molecular weight aggregates using hydroxyapatite chromatography |
| JP5199063B2 (ja) | 2005-03-11 | 2013-05-15 | ワイス・エルエルシー | 弱分配クロマトグラフィー方法 |
| KR101247836B1 (ko) * | 2005-06-17 | 2013-03-28 | 와이어쓰 엘엘씨 | 항 ａ 베타 항체의 정제 방법 |
| AU2006261920A1 (en) * | 2005-06-23 | 2007-01-04 | Medimmune, Llc | Antibody formulations having optimized aggregation and fragmentation profiles |
| AR058140A1 (es) * | 2005-10-24 | 2008-01-23 | Wyeth Corp | Metodo de produccion proteica utilizando compuestos anti-senescencia |
| BRPI0620316A2 (pt) | 2005-12-21 | 2011-11-08 | Wyeth Corp | formulações de proteìnas com viscosidades reduzida e seus usos |
| JPWO2007123242A1 (ja) | 2006-04-25 | 2009-09-10 | 東ソー株式会社 | ＩｇＧ精製用分離剤、及びそれを用いたＩｇＧ単量体の精製方法 |
| WO2009017491A1 (en) * | 2006-06-14 | 2009-02-05 | Smithkline Beecham Corporation | Methods for purifying antibodies using ceramic hydroxyapatite |
| CN101541825B (zh) * | 2006-08-28 | 2013-08-14 | 阿雷斯贸易股份有限公司 | Fc融合蛋白的纯化方法 |
| PL2061803T5 (pl) | 2006-08-28 | 2023-03-27 | Ares Trading S.A. | Proces oczyszczania białek zawierających fc |
| KR20090064377A (ko) * | 2006-09-08 | 2009-06-18 | 와이어쓰 | 친화성 크로마토그래피를 사용하는 단백질 정제에 있어서 아르기닌 세척 |
| RU2518289C2 (ru) | 2006-09-13 | 2014-06-10 | Эббви Инк, | Способ получения антитела или его фрагмента с подпиткой (варианты) |
| US8911964B2 (en) | 2006-09-13 | 2014-12-16 | Abbvie Inc. | Fed-batch method of making human anti-TNF-alpha antibody |
| US7999085B2 (en) * | 2007-01-09 | 2011-08-16 | Bio-Rad Laboratories, Inc. | Enhanced capacity and purification of protein by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers |
| US7691980B2 (en) * | 2007-01-09 | 2010-04-06 | Bio-Rad Laboratories, Inc. | Enhanced capacity and purification of antibodies by mixed mode chromatography in the presence of aqueous-soluble nonionic organic polymers |
| ES2366593T3 (es) * | 2007-01-26 | 2011-10-21 | Merck Serono S.A. | Purificación de proteínas de fusión taci-fc empleando la tecnología de cuerpos grasos. |
| TW200902708A (en) | 2007-04-23 | 2009-01-16 | Wyeth Corp | Methods of protein production using anti-senescence compounds |
| JP5778389B2 (ja) * | 2007-10-26 | 2015-09-16 | 旭化成ケミカルズ株式会社 | たんぱく質の精製方法 |
| CA2720615C (en) * | 2008-04-08 | 2016-06-07 | Bio-Rad Laboratories, Inc. | Chromatography purification of antibodies |
| FI3604324T3 (fi) | 2008-08-14 | 2024-05-16 | Genentech Inc | Menetelmiä epäpuhtauden poistamiseksi käyttäen luontaisen proteiinin syrjäytyksen ioninvaihtomembraanikromatografiaa |
| CA2932207A1 (en) * | 2008-10-20 | 2010-12-09 | Abbvie Inc. | Isolation and purification of antibodies using protein a affinity chromatography |
| EP2346897A2 (en) | 2008-10-20 | 2011-07-27 | Abbott Laboratories | Viral inactivation during purification of antibodies |
| BRPI0919979A2 (pt) | 2008-10-29 | 2015-12-15 | Wyeth Llc | formulações de moléculas de ligação de antígeno de domínio único |
| US10118962B2 (en) * | 2008-10-29 | 2018-11-06 | Ablynx N.V. | Methods for purification of single domain antigen binding molecules |
| WO2010051360A1 (en) * | 2008-10-31 | 2010-05-06 | Wyeth Llc | Purification of acidic proteins using ceramic hydroxyapatite chromatography |
| US20120141497A1 (en) * | 2009-03-11 | 2012-06-07 | Wyeth Llc | Methods of purifying small modular immunopharmaceutical proteins |
| US8481694B2 (en) | 2009-04-29 | 2013-07-09 | Bio-Rad Laboratories, Inc. | Purification of immunoconjugates |
| WO2011001963A1 (ja) | 2009-07-03 | 2011-01-06 | 旭化成ケミカルズ株式会社 | 多孔質基材に固定されたグラフト鎖に結合しているアミノ基及びアルキル基を有する多孔膜を用いた抗体の精製方法 |
| ES2813398T3 (es) * | 2009-10-20 | 2021-03-23 | Abbvie Inc | Aislamiento y purificación de anticuerpos anti-IL-13 usando cromatografía de afinidad a Proteína A |
| SG181771A1 (en) | 2009-12-18 | 2012-07-30 | Novartis Ag | Wash solution and method for affinity chromatography |
| WO2011090720A2 (en) * | 2009-12-29 | 2011-07-28 | Dr. Reddy's Laboratories Ltd | Purification of proteins |
| CA2787009C (en) | 2010-01-15 | 2018-04-03 | Bio-Rad Laboratories, Inc. | Surface neutralization of apatite |
| JP5737817B2 (ja) * | 2010-04-14 | 2015-06-17 | エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ | 免疫グロブリン凝集体の除去 |
| GB201006753D0 (en) | 2010-04-22 | 2010-06-09 | Biotest Ag | Process for preparing an immunolobulin composition |
| US20110301333A1 (en) * | 2010-06-08 | 2011-12-08 | Millipore Corporation | Removal of protein aggregates from biopharmaceutical preparations using calcium phosphate salts |
| US8895707B2 (en) | 2010-08-18 | 2014-11-25 | Bio-Rad Laboratories, Inc. | Elution of proteins from hydroxyapatite resins without resin deterioration |
| CA2814781A1 (en) | 2010-11-01 | 2012-05-10 | Diderik Reinder Kremer | Single unit ion exchange chromatography antibody purification |
| US9592540B2 (en) | 2011-02-02 | 2017-03-14 | Bio-Rad Laboratories, Inc. | Apatite surface neutralization with alkali solutions |
| CN103717284B (zh) * | 2011-06-08 | 2016-02-24 | 新加坡科技研究局 | 通过约束共水合色谱纯化生物制品 |
| BR112013031906A2 (pt) * | 2011-06-16 | 2016-12-13 | Dsm Ip Assets Bv | unidade única de cromatografia de purificação de anticorpo |
| SG10201701224UA (en) | 2012-03-12 | 2017-04-27 | Merck Patent Gmbh | Removal of protein aggregates from biopharmaceutical preparations in a flowthrough mode |
| US9815695B2 (en) | 2012-05-30 | 2017-11-14 | Bio-Rad Laboratories, Inc. | In situ restoration of apatite-based chromatography resins |
| WO2014003142A1 (ja) * | 2012-06-27 | 2014-01-03 | 旭化成メディカル株式会社 | 抗体 |
| JP2014105180A (ja) * | 2012-11-27 | 2014-06-09 | Hoya Corp | モノマー化方法 |
| JP6450364B2 (ja) | 2013-03-13 | 2019-01-09 | セセン バイオ， インコーポレイテッド | 眼送達のためのキメラサイトカイン製剤 |
| CN105324393A (zh) * | 2013-05-13 | 2016-02-10 | 米迪缪尼有限公司 | 具有最小单体分离的重组多克隆抗体多聚体的分离 |
| JP2016532100A (ja) | 2013-07-05 | 2016-10-13 | ラボラトワール・フランセ・デュ・フラクシオンマン・エ・デ・ビョテクノロジーＬａｂｏｒａｔｏｉｒｅ Ｆｒａｎｃａｉｓ Ｄｕ Ｆｒａｃｔｉｏｎｎｅｍｅｎｔ Ｅｔ Ｄｅｓ Ｂｉｏｔｅｃｈｎｏｌｏｇｉｅｓ | アフィニティークロマトグラフィーマトリックス |
| JP6479305B2 (ja) * | 2013-07-12 | 2019-03-06 | 株式会社Ｕｍｎファーマ | ウイルス様粒子の精製方法 |
| KR101847004B1 (ko) | 2013-07-12 | 2018-04-10 | 메르크 파텐트 게엠베하 | 표적 단백질을 함유하는 샘플로부터 단편들의 활성탄을 이용한 제거 |
| KR101891522B1 (ko) | 2013-12-12 | 2018-08-24 | 이엠디 밀리포어 코포레이션 | 아크릴아미드 함유 필터를 사용한 단백질 분리 |
| BR112016014824A2 (pt) * | 2013-12-27 | 2017-09-19 | Chugai Pharmaceutical Co Ltd | Método para purificar anticorpo que tem ponto isoelétrico baixo |
| US10099157B2 (en) | 2014-06-23 | 2018-10-16 | Bio-Rad Laboratories, Inc. | Apatite in-situ restoration |
| EP3157689B1 (en) * | 2014-06-23 | 2021-04-28 | Bio-Rad Laboratories, Inc. | Apatite pretreatment |
| CN107001411B (zh) | 2014-12-15 | 2021-07-23 | 默克专利股份公司 | 从粗溶液捕获靶分子 |
| AU2016233557B2 (en) * | 2015-03-13 | 2021-06-24 | Bristol-Myers Squibb Company | Use of alkaline washes during chromatography to remove impurities |
| WO2017109619A1 (en) | 2015-12-21 | 2017-06-29 | Pfizer Inc. | Purification of antibody drug conjugates using a sodium phosphate gradient |
| US11186858B1 (en) | 2016-03-15 | 2021-11-30 | Fresenius Kabi Deutschland Gmbh | Methods for increasing biosimilarity |
| US11919924B1 (en) * | 2016-03-15 | 2024-03-05 | Fresenius Kabi Deutschland Gmbh | Methods of purifying and producing an adalimumab antibody |
| CN106977591B (zh) * | 2017-05-04 | 2020-05-05 | 广州康盛生物科技股份有限公司 | 一种分离纯化重组葡萄球菌蛋白a的方法 |
| WO2019040671A1 (en) * | 2017-08-22 | 2019-02-28 | Biogen Ma Inc. | METHODS OF PURIFYING ANTIBODIES HAVING REDUCED AGGREGATES OF HIGH MOLECULAR WEIGHT |
| WO2019116096A1 (en) * | 2017-12-15 | 2019-06-20 | Laboratoire Francais Du Fractionnement Et Des Biotechnologies | Production of fc fragments |
| EP3759134A1 (en) * | 2018-02-27 | 2021-01-06 | Pfizer Inc | Antibody purification |
| CN111902720A (zh) | 2018-03-21 | 2020-11-06 | 沃特世科技公司 | 基于非抗体高亲和力的样品制备、吸附剂、装置和方法 |
| JP2021519339A (ja) | 2018-04-03 | 2021-08-10 | メルク パテント ゲーエムベーハー | Ｃｅｘクロマトグラフィー媒体及び生物製剤供給原料からの標的タンパク質の低塩溶出 |
| WO2019224660A1 (ja) * | 2018-05-24 | 2019-11-28 | ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 | 吸着剤の生産方法 |
| KR20220101168A (ko) | 2019-11-22 | 2022-07-19 | 모르포시스 아게 | 이온 교환 크로마토그래피 동안 항체의 수율을 증가시키는 방법 |
| JP2020099909A (ja) * | 2020-03-17 | 2020-07-02 | ＨＯＹＡ Ｔｅｃｈｎｏｓｕｒｇｉｃａｌ株式会社 | 処理方法、生産方法およびハイドロキシアパタイト充填剤 |
| US20230136595A1 (en) | 2020-03-30 | 2023-05-04 | Ablynx Nv | Method for the production and purification of multivalent immunoglobulin single variable domains |
| US20230167153A1 (en) * | 2020-05-01 | 2023-06-01 | Kashiv Biosciences, Llc | An improved process of purification of protein |
Family Cites Families (11)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US514018A (en) | 1894-02-06 | Edward j | ||
| US523335A (en) | 1894-07-24 | Air-bolt for flour-mills | ||
| US419964A (en) | 1890-01-21 | Transom-lifter | ||
| US428894A (en) | 1890-05-27 | Process of making tool-handles | ||
| US4745183A (en) * | 1984-02-21 | 1988-05-17 | Bio-Rad Laboratories, Inc. | Use of hydroxylapatite for the analysis and preparation of purified monoclonal antibodies |
| JPH03291300A (ja) * | 1989-12-28 | 1991-12-20 | Mitsui Toatsu Chem Inc | ポリクローナル抗体の分離方法 |
| GB9022545D0 (en) * | 1990-10-17 | 1990-11-28 | Wellcome Found | Culture medium |
| TWI255272B (en) * | 2000-12-06 | 2006-05-21 | Guriq Basi | Humanized antibodies that recognize beta amyloid peptide |
| AU2002359816B2 (en) * | 2001-12-21 | 2006-07-13 | Immunex Corporation | Methods for purifying protein |
| WO2004076485A1 (en) | 2003-02-28 | 2004-09-10 | Lonza Biologics Plc. | Antibody purification by protein a and ion exchange chromatography |
| CA2543193C (en) * | 2003-10-27 | 2015-08-11 | Wyeth | Removal of high molecular weight aggregates using hydroxyapatite chromatography |
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