DK174998B1 - Fermenteringsfremgangsmåde og anordning til udøvelse heraf - Google Patents
Fermenteringsfremgangsmåde og anordning til udøvelse heraf Download PDFInfo
- Publication number
- DK174998B1 DK174998B1 DK198806152A DK615288A DK174998B1 DK 174998 B1 DK174998 B1 DK 174998B1 DK 198806152 A DK198806152 A DK 198806152A DK 615288 A DK615288 A DK 615288A DK 174998 B1 DK174998 B1 DK 174998B1
- Authority
- DK
- Denmark
- Prior art keywords
- container
- microcarriers
- trypsin solution
- trypsin
- insert
- Prior art date
Links
- 238000000855 fermentation Methods 0.000 title 1
- 230000004151 fermentation Effects 0.000 title 1
- 102000004142 Trypsin Human genes 0.000 claims abstract description 35
- 108090000631 Trypsin Proteins 0.000 claims abstract description 35
- 239000012588 trypsin Substances 0.000 claims abstract description 35
- 238000000034 method Methods 0.000 claims abstract description 20
- 238000004113 cell culture Methods 0.000 claims abstract description 13
- 239000000243 solution Substances 0.000 claims description 29
- 239000011534 wash buffer Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 5
- 239000000969 carrier Substances 0.000 claims description 3
- 230000000415 inactivating effect Effects 0.000 claims 1
- 238000010079 rubber tapping Methods 0.000 claims 1
- 238000007789 sealing Methods 0.000 claims 1
- 230000003248 secreting effect Effects 0.000 claims 1
- 230000000694 effects Effects 0.000 abstract description 3
- 230000002411 adverse Effects 0.000 abstract description 2
- 238000005112 continuous flow technique Methods 0.000 abstract 1
- 101710131583 Trypsin-10 Proteins 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000001627 detrimental effect Effects 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 230000035939 shock Effects 0.000 description 1
- 238000003756 stirring Methods 0.000 description 1
- 239000000725 suspension Substances 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Wood Science & Technology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Cell Biology (AREA)
- Immunology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19873739649 DE3739649A1 (de) | 1987-11-23 | 1987-11-23 | Verfahren zum abloesen von zellkulturen von mikrotraegern |
| DE3739649 | 1987-11-23 |
Publications (3)
| Publication Number | Publication Date |
|---|---|
| DK615288D0 DK615288D0 (da) | 1988-11-03 |
| DK615288A DK615288A (da) | 1989-05-24 |
| DK174998B1 true DK174998B1 (da) | 2004-04-13 |
Family
ID=6341058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK198806152A DK174998B1 (da) | 1987-11-23 | 1988-11-03 | Fermenteringsfremgangsmåde og anordning til udøvelse heraf |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5100799A (OSRAM) |
| EP (1) | EP0317810B1 (OSRAM) |
| JP (1) | JPH0740929B2 (OSRAM) |
| AT (1) | ATE110411T1 (OSRAM) |
| CA (1) | CA1322541C (OSRAM) |
| DE (2) | DE3739649A1 (OSRAM) |
| DK (1) | DK174998B1 (OSRAM) |
| ES (1) | ES2063016T3 (OSRAM) |
| FI (1) | FI89506C (OSRAM) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| JP3246664B2 (ja) * | 1993-01-29 | 2002-01-15 | ニュー ブルンズウイック サイエンティフィック カンパニー インコーポレイテッド | 足場および懸濁細胞の培養方法並びにその装置 |
| US6378527B1 (en) * | 1998-04-08 | 2002-04-30 | Chondros, Inc. | Cell-culture and polymer constructs |
| US20030104494A1 (en) * | 2001-10-26 | 2003-06-05 | Ilya Ravkin | Assay systems with adjustable fluid communication |
| US20080187949A1 (en) * | 2001-10-26 | 2008-08-07 | Millipore Corporation | Multiplexed assays of cell migration |
| US20040126773A1 (en) * | 2002-05-23 | 2004-07-01 | Beske Oren E. | Assays with coded sensor particles to sense assay conditions |
| CN1300297C (zh) * | 2002-09-20 | 2007-02-14 | 华东理工大学 | 一种用于动物细胞扩大接种的消化反应器 |
| US20080207465A1 (en) * | 2002-10-28 | 2008-08-28 | Millipore Corporation | Assay systems with adjustable fluid communication |
| US20090137018A1 (en) * | 2003-06-30 | 2009-05-28 | University Of South Florida | Magnetic three-dimensional cell culture apparatus and method |
| US20050054101A1 (en) * | 2003-07-17 | 2005-03-10 | Global Cell Solutions Llc | Automated cell culture system and process |
| WO2005010139A2 (en) | 2003-07-23 | 2005-02-03 | University Of South Florida | Ferromagnetic cell and tissue culture microcarriers |
| US8691565B2 (en) | 2008-03-05 | 2014-04-08 | Terumo Bct, Inc. | Method of reseeding adherent cells grown in a hollow fiber bioreactor system |
| WO2011047900A2 (en) | 2009-10-22 | 2011-04-28 | Glaxosmithkline Biologicals S.A. | Novel process |
| CN101775364A (zh) * | 2010-03-05 | 2010-07-14 | 广州齐志生物工程设备有限公司 | 一种动物细胞的分离方法及分离装置 |
| EP3190175B1 (en) | 2013-06-24 | 2018-08-08 | Corning Incorporated | Cell culture article and methods thereof |
| US10260036B2 (en) | 2014-10-17 | 2019-04-16 | Sani-Tech West, Inc. | Mixing and filtering system and method |
| ES2762977T3 (es) | 2015-05-29 | 2020-05-26 | Mesoblast Int Sarl | Procedimientos y dispositivos para separar células de microportadores |
| CN105238689A (zh) * | 2015-09-29 | 2016-01-13 | 中牧实业股份有限公司 | 一种吹打细胞的装置及基于其的细胞传代培养方法 |
| WO2020150078A1 (en) | 2019-01-16 | 2020-07-23 | Corning Incorporated | Systems and methods for culturing cells in suspension |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2300567A1 (de) * | 1973-01-08 | 1974-07-11 | Eberhard Dr Med Passarge | Verfahren und einrichtung zum zuechten diploider fibroblasten oder aehnlicher zellen |
| US4144126A (en) * | 1975-05-21 | 1979-03-13 | Beecham Group Limited | Cell culture method |
| US4201845A (en) * | 1976-04-12 | 1980-05-06 | Monsanto Company | Cell culture reactor |
| GB2006181B (en) * | 1977-10-20 | 1982-05-19 | Hartley Simon Ltd | Growth of biological material |
| US4237218A (en) * | 1979-02-09 | 1980-12-02 | Bio-Rad Laboratories, Inc. | Micro-carrier cell culture |
| SE445116B (sv) * | 1979-09-12 | 1986-06-02 | Pharmacia Fine Chemicals Ab | Sett att odla celler pa mikroberare med fibronektinytskikt |
| JPS60259179A (ja) * | 1984-06-05 | 1985-12-21 | Teijin Ltd | 細胞培養槽及び細胞培養方法 |
| WO1986001531A1 (en) * | 1984-08-28 | 1986-03-13 | Massachusetts Institute Of Technology | Detachment of anchorage-dependent cells from microcarriers |
-
1987
- 1987-11-23 DE DE19873739649 patent/DE3739649A1/de active Granted
-
1988
- 1988-11-03 CA CA000582141A patent/CA1322541C/en not_active Expired - Fee Related
- 1988-11-03 DK DK198806152A patent/DK174998B1/da not_active IP Right Cessation
- 1988-11-04 EP EP88118431A patent/EP0317810B1/de not_active Expired - Lifetime
- 1988-11-04 DE DE3851206T patent/DE3851206D1/de not_active Expired - Fee Related
- 1988-11-04 AT AT88118431T patent/ATE110411T1/de not_active IP Right Cessation
- 1988-11-04 ES ES88118431T patent/ES2063016T3/es not_active Expired - Lifetime
- 1988-11-21 US US07/274,430 patent/US5100799A/en not_active Expired - Lifetime
- 1988-11-22 JP JP63295877A patent/JPH0740929B2/ja not_active Expired - Fee Related
- 1988-11-23 FI FI885435A patent/FI89506C/fi not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| FI89506C (fi) | 1993-10-11 |
| DE3851206D1 (de) | 1994-09-29 |
| ES2063016T3 (es) | 1995-01-01 |
| DK615288D0 (da) | 1988-11-03 |
| ATE110411T1 (de) | 1994-09-15 |
| EP0317810A3 (de) | 1991-02-06 |
| FI885435A7 (fi) | 1989-05-24 |
| JPH0740929B2 (ja) | 1995-05-10 |
| EP0317810A2 (de) | 1989-05-31 |
| JPH01168273A (ja) | 1989-07-03 |
| DE3739649A1 (de) | 1989-06-22 |
| DE3739649C2 (OSRAM) | 1989-11-02 |
| DK615288A (da) | 1989-05-24 |
| EP0317810B1 (de) | 1994-08-24 |
| CA1322541C (en) | 1993-09-28 |
| US5100799A (en) | 1992-03-31 |
| FI885435A0 (fi) | 1988-11-23 |
| FI89506B (fi) | 1993-06-30 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK174998B1 (da) | Fermenteringsfremgangsmåde og anordning til udøvelse heraf | |
| US5707868A (en) | Variable-volume reactor-type device and process for culturing cellular material | |
| GB2314343A (en) | Apparatus for intermittently exposing cells during culture | |
| KR101925778B1 (ko) | 세포 배양 방법 및 세포 배양 시스템 | |
| KR102427715B1 (ko) | 교번 접선 유동식의 신속한 수확 | |
| JP2005218413A (ja) | 細胞培養装置 | |
| KR102535013B1 (ko) | 정유량 펌프/튜빙 시스템이 있는 고정층 바이오 리액터 | |
| JP4775218B2 (ja) | 閉鎖系の細胞回収装置及び細胞培養装置並びに細胞の回収方法及び培養方法 | |
| JP2015188391A (ja) | 細胞培養方法、及び細胞培養システム | |
| US4339537A (en) | Method of culturing biological substances | |
| JP2005278564A (ja) | 細胞培養装置 | |
| JP2980867B2 (ja) | 細胞の培養方法及び培養装置 | |
| JP4928831B2 (ja) | 自動培養装置 | |
| JP7030849B2 (ja) | 細胞培養装置及び細胞培養方法 | |
| EP3303549A1 (en) | Methods and apparatus for separating cells from microcarriers | |
| TW202506987A (zh) | 細胞培養裝置 | |
| WO2021049117A1 (ja) | 細胞培養装置 | |
| JP6303497B2 (ja) | 細胞剥離回収装置及び細胞剥離回収方法及び細胞培養システム | |
| JP2011083263A (ja) | 細胞培養装置及び細胞培養方法 | |
| CN113166698B (zh) | 用于灌注培养的设备和方法 | |
| CN207193165U (zh) | 用于合成肽树脂洗涤的装置 | |
| JP7634938B2 (ja) | バイオリアクターにおける治療細胞の膨張および分化のためのシステムおよび方法 | |
| JP6349949B2 (ja) | 担体供給方法及び担体供給装置及び細胞培養システム | |
| JPS6147184A (ja) | 体細胞集積浮遊培養法及びそれに使用する培養器 | |
| JP2023124468A (ja) | 細胞分離装置および細胞分離方法 |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PBP | Patent lapsed |
Country of ref document: DK |