DE3739649C2 - - Google Patents

Info

Publication number
DE3739649C2
DE3739649C2 DE3739649A DE3739649A DE3739649C2 DE 3739649 C2 DE3739649 C2 DE 3739649C2 DE 3739649 A DE3739649 A DE 3739649A DE 3739649 A DE3739649 A DE 3739649A DE 3739649 C2 DE3739649 C2 DE 3739649C2
Authority
DE
Germany
Prior art keywords
container
microcarriers
trypsin solution
trypsin
insert
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
DE3739649A
Other languages
German (de)
English (en)
Other versions
DE3739649A1 (de
Inventor
Wolfgang Dr. Wien At Mundt
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Oesterreichisches Institut fuer Haemoderivate
Original Assignee
Immuno AG fuer Chemisch Medizinische Produkte
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Immuno AG fuer Chemisch Medizinische Produkte filed Critical Immuno AG fuer Chemisch Medizinische Produkte
Priority to DE19873739649 priority Critical patent/DE3739649A1/de
Priority to DK198806152A priority patent/DK174998B1/da
Priority to CA000582141A priority patent/CA1322541C/en
Priority to EP88118431A priority patent/EP0317810B1/de
Priority to ES88118431T priority patent/ES2063016T3/es
Priority to DE3851206T priority patent/DE3851206D1/de
Priority to AT88118431T priority patent/ATE110411T1/de
Priority to US07/274,430 priority patent/US5100799A/en
Priority to JP63295877A priority patent/JPH0740929B2/ja
Priority to FI885435A priority patent/FI89506C/fi
Publication of DE3739649A1 publication Critical patent/DE3739649A1/de
Application granted granted Critical
Publication of DE3739649C2 publication Critical patent/DE3739649C2/de
Granted legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/04—Cell isolation or sorting
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Sustainable Development (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE19873739649 1987-11-23 1987-11-23 Verfahren zum abloesen von zellkulturen von mikrotraegern Granted DE3739649A1 (de)

Priority Applications (10)

Application Number Priority Date Filing Date Title
DE19873739649 DE3739649A1 (de) 1987-11-23 1987-11-23 Verfahren zum abloesen von zellkulturen von mikrotraegern
CA000582141A CA1322541C (en) 1987-11-23 1988-11-03 Method for releasing cell cultures from the microcarriers
DK198806152A DK174998B1 (da) 1987-11-23 1988-11-03 Fermenteringsfremgangsmåde og anordning til udøvelse heraf
ES88118431T ES2063016T3 (es) 1987-11-23 1988-11-04 Procedimiento y aparato para separar cultivos celulares de sus microsoportes.
EP88118431A EP0317810B1 (de) 1987-11-23 1988-11-04 Verfahren und Vorrichtung zum Ablösen von Zellkulturen von Mikroträgern
DE3851206T DE3851206D1 (de) 1987-11-23 1988-11-04 Verfahren und Vorrichtung zum Ablösen von Zellkulturen von Mikroträgern.
AT88118431T ATE110411T1 (de) 1987-11-23 1988-11-04 Verfahren und vorrichtung zum ablösen von zellkulturen von mikroträgern.
US07/274,430 US5100799A (en) 1987-11-23 1988-11-21 Method for releasing cell cultures from microcarriers
JP63295877A JPH0740929B2 (ja) 1987-11-23 1988-11-22 細胞培養生成物をマイクロキャリアーから分離する方法及び該方法を実施するための細胞培養生成物増殖用装置
FI885435A FI89506C (fi) 1987-11-23 1988-11-23 Foerfarande foer att frigoera cellkulturer fraon mikrobaerare

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
DE19873739649 DE3739649A1 (de) 1987-11-23 1987-11-23 Verfahren zum abloesen von zellkulturen von mikrotraegern

Publications (2)

Publication Number Publication Date
DE3739649A1 DE3739649A1 (de) 1989-06-22
DE3739649C2 true DE3739649C2 (OSRAM) 1989-11-02

Family

ID=6341058

Family Applications (2)

Application Number Title Priority Date Filing Date
DE19873739649 Granted DE3739649A1 (de) 1987-11-23 1987-11-23 Verfahren zum abloesen von zellkulturen von mikrotraegern
DE3851206T Expired - Fee Related DE3851206D1 (de) 1987-11-23 1988-11-04 Verfahren und Vorrichtung zum Ablösen von Zellkulturen von Mikroträgern.

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE3851206T Expired - Fee Related DE3851206D1 (de) 1987-11-23 1988-11-04 Verfahren und Vorrichtung zum Ablösen von Zellkulturen von Mikroträgern.

Country Status (9)

Country Link
US (1) US5100799A (OSRAM)
EP (1) EP0317810B1 (OSRAM)
JP (1) JPH0740929B2 (OSRAM)
AT (1) ATE110411T1 (OSRAM)
CA (1) CA1322541C (OSRAM)
DE (2) DE3739649A1 (OSRAM)
DK (1) DK174998B1 (OSRAM)
ES (1) ES2063016T3 (OSRAM)
FI (1) FI89506C (OSRAM)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0682697A4 (en) * 1993-01-29 1997-07-30 New Brunswick Scientific Co METHOD AND APPARATUS FOR CULTURING ANCHOR AND SUSPENSION CELLS.
US6378527B1 (en) * 1998-04-08 2002-04-30 Chondros, Inc. Cell-culture and polymer constructs
WO2003036265A2 (en) * 2001-10-26 2003-05-01 Virtual Arrays, Inc. Assay systems with adjustable fluid communication
US20080187949A1 (en) * 2001-10-26 2008-08-07 Millipore Corporation Multiplexed assays of cell migration
US20040126773A1 (en) * 2002-05-23 2004-07-01 Beske Oren E. Assays with coded sensor particles to sense assay conditions
CN1300297C (zh) * 2002-09-20 2007-02-14 华东理工大学 一种用于动物细胞扩大接种的消化反应器
US20080207465A1 (en) * 2002-10-28 2008-08-28 Millipore Corporation Assay systems with adjustable fluid communication
US20090137018A1 (en) * 2003-06-30 2009-05-28 University Of South Florida Magnetic three-dimensional cell culture apparatus and method
EP1660629A4 (en) * 2003-07-17 2015-04-08 Global Cell Solutions Llc SYSTEM AND METHOD FOR AUTOMATIC CELL CULTURE
WO2005010139A2 (en) 2003-07-23 2005-02-03 University Of South Florida Ferromagnetic cell and tissue culture microcarriers
WO2011047900A2 (en) 2009-10-22 2011-04-28 Glaxosmithkline Biologicals S.A. Novel process
CN101775364A (zh) * 2010-03-05 2010-07-14 广州齐志生物工程设备有限公司 一种动物细胞的分离方法及分离装置
CN105154394B (zh) * 2010-05-05 2019-06-18 泰尔茂比司特公司 重新接种中空纤维生物反应器系统中生长的贴壁细胞的方法
JP6632975B2 (ja) 2013-06-24 2020-01-22 コーニング インコーポレイテッド 細胞培養物品およびその方法
EP3207119B1 (en) 2014-10-17 2020-02-19 Sani-tech West, Inc. Mixing and filtering system
US11242506B2 (en) 2015-05-29 2022-02-08 Mesoblast International Sárl Methods and apparatus for separating cells from microcarriers
CN105238689A (zh) * 2015-09-29 2016-01-13 中牧实业股份有限公司 一种吹打细胞的装置及基于其的细胞传代培养方法
US20220073868A1 (en) 2019-01-16 2022-03-10 Corning Incorporated Systems and methods for culturing cells in suspension

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2300567A1 (de) * 1973-01-08 1974-07-11 Eberhard Dr Med Passarge Verfahren und einrichtung zum zuechten diploider fibroblasten oder aehnlicher zellen
US4144126A (en) * 1975-05-21 1979-03-13 Beecham Group Limited Cell culture method
US4201845A (en) * 1976-04-12 1980-05-06 Monsanto Company Cell culture reactor
GB2006181B (en) * 1977-10-20 1982-05-19 Hartley Simon Ltd Growth of biological material
US4237218A (en) * 1979-02-09 1980-12-02 Bio-Rad Laboratories, Inc. Micro-carrier cell culture
SE445116B (sv) * 1979-09-12 1986-06-02 Pharmacia Fine Chemicals Ab Sett att odla celler pa mikroberare med fibronektinytskikt
JPS60259179A (ja) * 1984-06-05 1985-12-21 Teijin Ltd 細胞培養槽及び細胞培養方法
EP0192705A1 (en) * 1984-08-28 1986-09-03 Massachusetts Institute Of Technology Detachment of anchorage-dependent cells from microcarriers

Also Published As

Publication number Publication date
FI885435A0 (fi) 1988-11-23
EP0317810A3 (de) 1991-02-06
EP0317810B1 (de) 1994-08-24
DE3739649A1 (de) 1989-06-22
DK174998B1 (da) 2004-04-13
FI89506B (fi) 1993-06-30
DK615288A (da) 1989-05-24
DK615288D0 (da) 1988-11-03
JPH01168273A (ja) 1989-07-03
EP0317810A2 (de) 1989-05-31
DE3851206D1 (de) 1994-09-29
FI89506C (fi) 1993-10-11
US5100799A (en) 1992-03-31
CA1322541C (en) 1993-09-28
ES2063016T3 (es) 1995-01-01
FI885435A7 (fi) 1989-05-24
ATE110411T1 (de) 1994-09-15
JPH0740929B2 (ja) 1995-05-10

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Legal Events

Date Code Title Description
OP8 Request for examination as to paragraph 44 patent law
D2 Grant after examination
8364 No opposition during term of opposition
8339 Ceased/non-payment of the annual fee