CA1322541C - Method for releasing cell cultures from the microcarriers - Google Patents
Method for releasing cell cultures from the microcarriersInfo
- Publication number
- CA1322541C CA1322541C CA000582141A CA582141A CA1322541C CA 1322541 C CA1322541 C CA 1322541C CA 000582141 A CA000582141 A CA 000582141A CA 582141 A CA582141 A CA 582141A CA 1322541 C CA1322541 C CA 1322541C
- Authority
- CA
- Canada
- Prior art keywords
- container
- microcarriers
- trypsin solution
- insert
- trypsin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 26
- 238000004113 cell culture Methods 0.000 title claims abstract description 18
- 102000004142 Trypsin Human genes 0.000 claims abstract description 40
- 108090000631 Trypsin Proteins 0.000 claims abstract description 40
- 239000012588 trypsin Substances 0.000 claims abstract description 40
- 239000000243 solution Substances 0.000 claims description 35
- 239000011534 wash buffer Substances 0.000 claims description 8
- 239000007788 liquid Substances 0.000 claims description 6
- 239000000725 suspension Substances 0.000 claims description 3
- 230000000415 inactivating effect Effects 0.000 claims description 2
- 238000005070 sampling Methods 0.000 claims description 2
- 230000002411 adverse Effects 0.000 description 3
- 230000003213 activating effect Effects 0.000 description 1
- 239000000969 carrier Substances 0.000 description 1
- 239000006285 cell suspension Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 230000018109 developmental process Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 238000002360 preparation method Methods 0.000 description 1
- 230000001902 propagating effect Effects 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE19873739649 DE3739649A1 (de) | 1987-11-23 | 1987-11-23 | Verfahren zum abloesen von zellkulturen von mikrotraegern |
| DEP3739649.8-41 | 1987-11-23 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CA1322541C true CA1322541C (en) | 1993-09-28 |
Family
ID=6341058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CA000582141A Expired - Fee Related CA1322541C (en) | 1987-11-23 | 1988-11-03 | Method for releasing cell cultures from the microcarriers |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5100799A (OSRAM) |
| EP (1) | EP0317810B1 (OSRAM) |
| JP (1) | JPH0740929B2 (OSRAM) |
| AT (1) | ATE110411T1 (OSRAM) |
| CA (1) | CA1322541C (OSRAM) |
| DE (2) | DE3739649A1 (OSRAM) |
| DK (1) | DK174998B1 (OSRAM) |
| ES (1) | ES2063016T3 (OSRAM) |
| FI (1) | FI89506C (OSRAM) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0682697A4 (en) * | 1993-01-29 | 1997-07-30 | New Brunswick Scientific Co | METHOD AND APPARATUS FOR CULTURING ANCHOR AND SUSPENSION CELLS. |
| US6378527B1 (en) * | 1998-04-08 | 2002-04-30 | Chondros, Inc. | Cell-culture and polymer constructs |
| WO2003036265A2 (en) * | 2001-10-26 | 2003-05-01 | Virtual Arrays, Inc. | Assay systems with adjustable fluid communication |
| US20080187949A1 (en) * | 2001-10-26 | 2008-08-07 | Millipore Corporation | Multiplexed assays of cell migration |
| US20040126773A1 (en) * | 2002-05-23 | 2004-07-01 | Beske Oren E. | Assays with coded sensor particles to sense assay conditions |
| CN1300297C (zh) * | 2002-09-20 | 2007-02-14 | 华东理工大学 | 一种用于动物细胞扩大接种的消化反应器 |
| US20080207465A1 (en) * | 2002-10-28 | 2008-08-28 | Millipore Corporation | Assay systems with adjustable fluid communication |
| US20090137018A1 (en) * | 2003-06-30 | 2009-05-28 | University Of South Florida | Magnetic three-dimensional cell culture apparatus and method |
| EP1660629A4 (en) * | 2003-07-17 | 2015-04-08 | Global Cell Solutions Llc | SYSTEM AND METHOD FOR AUTOMATIC CELL CULTURE |
| WO2005010139A2 (en) | 2003-07-23 | 2005-02-03 | University Of South Florida | Ferromagnetic cell and tissue culture microcarriers |
| WO2011047900A2 (en) | 2009-10-22 | 2011-04-28 | Glaxosmithkline Biologicals S.A. | Novel process |
| CN101775364A (zh) * | 2010-03-05 | 2010-07-14 | 广州齐志生物工程设备有限公司 | 一种动物细胞的分离方法及分离装置 |
| CN105154394B (zh) * | 2010-05-05 | 2019-06-18 | 泰尔茂比司特公司 | 重新接种中空纤维生物反应器系统中生长的贴壁细胞的方法 |
| JP6632975B2 (ja) | 2013-06-24 | 2020-01-22 | コーニング インコーポレイテッド | 細胞培養物品およびその方法 |
| EP3207119B1 (en) | 2014-10-17 | 2020-02-19 | Sani-tech West, Inc. | Mixing and filtering system |
| US11242506B2 (en) | 2015-05-29 | 2022-02-08 | Mesoblast International Sárl | Methods and apparatus for separating cells from microcarriers |
| CN105238689A (zh) * | 2015-09-29 | 2016-01-13 | 中牧实业股份有限公司 | 一种吹打细胞的装置及基于其的细胞传代培养方法 |
| US20220073868A1 (en) | 2019-01-16 | 2022-03-10 | Corning Incorporated | Systems and methods for culturing cells in suspension |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2300567A1 (de) * | 1973-01-08 | 1974-07-11 | Eberhard Dr Med Passarge | Verfahren und einrichtung zum zuechten diploider fibroblasten oder aehnlicher zellen |
| US4144126A (en) * | 1975-05-21 | 1979-03-13 | Beecham Group Limited | Cell culture method |
| US4201845A (en) * | 1976-04-12 | 1980-05-06 | Monsanto Company | Cell culture reactor |
| GB2006181B (en) * | 1977-10-20 | 1982-05-19 | Hartley Simon Ltd | Growth of biological material |
| US4237218A (en) * | 1979-02-09 | 1980-12-02 | Bio-Rad Laboratories, Inc. | Micro-carrier cell culture |
| SE445116B (sv) * | 1979-09-12 | 1986-06-02 | Pharmacia Fine Chemicals Ab | Sett att odla celler pa mikroberare med fibronektinytskikt |
| JPS60259179A (ja) * | 1984-06-05 | 1985-12-21 | Teijin Ltd | 細胞培養槽及び細胞培養方法 |
| EP0192705A1 (en) * | 1984-08-28 | 1986-09-03 | Massachusetts Institute Of Technology | Detachment of anchorage-dependent cells from microcarriers |
-
1987
- 1987-11-23 DE DE19873739649 patent/DE3739649A1/de active Granted
-
1988
- 1988-11-03 DK DK198806152A patent/DK174998B1/da not_active IP Right Cessation
- 1988-11-03 CA CA000582141A patent/CA1322541C/en not_active Expired - Fee Related
- 1988-11-04 AT AT88118431T patent/ATE110411T1/de not_active IP Right Cessation
- 1988-11-04 DE DE3851206T patent/DE3851206D1/de not_active Expired - Fee Related
- 1988-11-04 EP EP88118431A patent/EP0317810B1/de not_active Expired - Lifetime
- 1988-11-04 ES ES88118431T patent/ES2063016T3/es not_active Expired - Lifetime
- 1988-11-21 US US07/274,430 patent/US5100799A/en not_active Expired - Lifetime
- 1988-11-22 JP JP63295877A patent/JPH0740929B2/ja not_active Expired - Fee Related
- 1988-11-23 FI FI885435A patent/FI89506C/fi not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| FI885435A0 (fi) | 1988-11-23 |
| EP0317810A3 (de) | 1991-02-06 |
| EP0317810B1 (de) | 1994-08-24 |
| DE3739649A1 (de) | 1989-06-22 |
| DK174998B1 (da) | 2004-04-13 |
| FI89506B (fi) | 1993-06-30 |
| DK615288A (da) | 1989-05-24 |
| DK615288D0 (da) | 1988-11-03 |
| JPH01168273A (ja) | 1989-07-03 |
| EP0317810A2 (de) | 1989-05-31 |
| DE3851206D1 (de) | 1994-09-29 |
| FI89506C (fi) | 1993-10-11 |
| US5100799A (en) | 1992-03-31 |
| ES2063016T3 (es) | 1995-01-01 |
| DE3739649C2 (OSRAM) | 1989-11-02 |
| FI885435A7 (fi) | 1989-05-24 |
| ATE110411T1 (de) | 1994-09-15 |
| JPH0740929B2 (ja) | 1995-05-10 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| MKLA | Lapsed |