JPH0740929B2 - 細胞培養生成物をマイクロキャリアーから分離する方法及び該方法を実施するための細胞培養生成物増殖用装置 - Google Patents
細胞培養生成物をマイクロキャリアーから分離する方法及び該方法を実施するための細胞培養生成物増殖用装置Info
- Publication number
- JPH0740929B2 JPH0740929B2 JP63295877A JP29587788A JPH0740929B2 JP H0740929 B2 JPH0740929 B2 JP H0740929B2 JP 63295877 A JP63295877 A JP 63295877A JP 29587788 A JP29587788 A JP 29587788A JP H0740929 B2 JPH0740929 B2 JP H0740929B2
- Authority
- JP
- Japan
- Prior art keywords
- container
- microcarriers
- trypsin solution
- cell culture
- trypsin
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
- 238000000034 method Methods 0.000 title claims abstract description 31
- 238000004113 cell culture Methods 0.000 title claims abstract description 19
- 102000004142 Trypsin Human genes 0.000 claims abstract description 37
- 108090000631 Trypsin Proteins 0.000 claims abstract description 37
- 239000012588 trypsin Substances 0.000 claims abstract description 37
- 239000000243 solution Substances 0.000 claims description 30
- 239000007788 liquid Substances 0.000 claims description 15
- 239000000725 suspension Substances 0.000 claims description 4
- 238000005070 sampling Methods 0.000 claims description 3
- 230000000415 inactivating effect Effects 0.000 claims description 2
- 239000011534 wash buffer Substances 0.000 claims 2
- 238000007599 discharging Methods 0.000 claims 1
- 230000002411 adverse Effects 0.000 abstract description 4
- 238000005112 continuous flow technique Methods 0.000 abstract 1
- 230000000694 effects Effects 0.000 abstract 1
- 238000004140 cleaning Methods 0.000 description 5
- 230000008901 benefit Effects 0.000 description 4
- 238000000926 separation method Methods 0.000 description 4
- 239000007853 buffer solution Substances 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 239000000872 buffer Substances 0.000 description 2
- 238000010923 batch production Methods 0.000 description 1
- 230000010261 cell growth Effects 0.000 description 1
- 238000009826 distribution Methods 0.000 description 1
- 230000012010 growth Effects 0.000 description 1
- 239000001963 growth medium Substances 0.000 description 1
- 239000002609 medium Substances 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 230000001376 precipitating effect Effects 0.000 description 1
- 238000004064 recycling Methods 0.000 description 1
- 238000009827 uniform distribution Methods 0.000 description 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
- C12M47/04—Cell isolation or sorting
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
- C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
- C12M25/16—Particles; Beads; Granular material; Encapsulation
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Biochemistry (AREA)
- Sustainable Development (AREA)
- Microbiology (AREA)
- Biomedical Technology (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Immunology (AREA)
- Cell Biology (AREA)
- Molecular Biology (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Transition And Organic Metals Composition Catalysts For Addition Polymerization (AREA)
- Medicines Containing Material From Animals Or Micro-Organisms (AREA)
- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| DE3739649.8 | 1987-11-23 | ||
| DE19873739649 DE3739649A1 (de) | 1987-11-23 | 1987-11-23 | Verfahren zum abloesen von zellkulturen von mikrotraegern |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| JPH01168273A JPH01168273A (ja) | 1989-07-03 |
| JPH0740929B2 true JPH0740929B2 (ja) | 1995-05-10 |
Family
ID=6341058
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| JP63295877A Expired - Fee Related JPH0740929B2 (ja) | 1987-11-23 | 1988-11-22 | 細胞培養生成物をマイクロキャリアーから分離する方法及び該方法を実施するための細胞培養生成物増殖用装置 |
Country Status (9)
| Country | Link |
|---|---|
| US (1) | US5100799A (OSRAM) |
| EP (1) | EP0317810B1 (OSRAM) |
| JP (1) | JPH0740929B2 (OSRAM) |
| AT (1) | ATE110411T1 (OSRAM) |
| CA (1) | CA1322541C (OSRAM) |
| DE (2) | DE3739649A1 (OSRAM) |
| DK (1) | DK174998B1 (OSRAM) |
| ES (1) | ES2063016T3 (OSRAM) |
| FI (1) | FI89506C (OSRAM) |
Families Citing this family (18)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO1994017178A1 (en) * | 1993-01-29 | 1994-08-04 | New Brunswick Scientific Co., Inc. | Method and apparatus for anchorage and suspension cell culture |
| US6378527B1 (en) * | 1998-04-08 | 2002-04-30 | Chondros, Inc. | Cell-culture and polymer constructs |
| US20030104494A1 (en) * | 2001-10-26 | 2003-06-05 | Ilya Ravkin | Assay systems with adjustable fluid communication |
| US20080187949A1 (en) * | 2001-10-26 | 2008-08-07 | Millipore Corporation | Multiplexed assays of cell migration |
| US20040126773A1 (en) * | 2002-05-23 | 2004-07-01 | Beske Oren E. | Assays with coded sensor particles to sense assay conditions |
| CN1300297C (zh) * | 2002-09-20 | 2007-02-14 | 华东理工大学 | 一种用于动物细胞扩大接种的消化反应器 |
| US20080207465A1 (en) * | 2002-10-28 | 2008-08-28 | Millipore Corporation | Assay systems with adjustable fluid communication |
| US20090137018A1 (en) | 2003-06-30 | 2009-05-28 | University Of South Florida | Magnetic three-dimensional cell culture apparatus and method |
| WO2005010162A2 (en) * | 2003-07-17 | 2005-02-03 | Global Cell Solutions, Llc. | Automated cell culture system and process |
| WO2005010139A2 (en) | 2003-07-23 | 2005-02-03 | University Of South Florida | Ferromagnetic cell and tissue culture microcarriers |
| WO2011047900A2 (en) | 2009-10-22 | 2011-04-28 | Glaxosmithkline Biologicals S.A. | Novel process |
| CN101775364A (zh) * | 2010-03-05 | 2010-07-14 | 广州齐志生物工程设备有限公司 | 一种动物细胞的分离方法及分离装置 |
| CN102869762B (zh) | 2010-05-05 | 2015-09-02 | 泰尔茂比司特公司 | 重新接种中空纤维生物反应器系统中生长的贴壁细胞的方法 |
| EP3190175B1 (en) | 2013-06-24 | 2018-08-08 | Corning Incorporated | Cell culture article and methods thereof |
| EP3207119B1 (en) | 2014-10-17 | 2020-02-19 | Sani-tech West, Inc. | Mixing and filtering system |
| WO2016193150A1 (en) | 2015-05-29 | 2016-12-08 | Mesoblast International Sàrl | Methods and apparatus for separating cells from microcarriers |
| CN105238689A (zh) * | 2015-09-29 | 2016-01-13 | 中牧实业股份有限公司 | 一种吹打细胞的装置及基于其的细胞传代培养方法 |
| CN113574165B (zh) | 2019-01-16 | 2024-08-13 | 康宁股份有限公司 | 用于悬浮培养细胞的系统和方法 |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DE2300567A1 (de) * | 1973-01-08 | 1974-07-11 | Eberhard Dr Med Passarge | Verfahren und einrichtung zum zuechten diploider fibroblasten oder aehnlicher zellen |
| US4144126A (en) * | 1975-05-21 | 1979-03-13 | Beecham Group Limited | Cell culture method |
| US4201845A (en) * | 1976-04-12 | 1980-05-06 | Monsanto Company | Cell culture reactor |
| GB2006181B (en) * | 1977-10-20 | 1982-05-19 | Hartley Simon Ltd | Growth of biological material |
| US4237218A (en) * | 1979-02-09 | 1980-12-02 | Bio-Rad Laboratories, Inc. | Micro-carrier cell culture |
| SE445116B (sv) * | 1979-09-12 | 1986-06-02 | Pharmacia Fine Chemicals Ab | Sett att odla celler pa mikroberare med fibronektinytskikt |
| JPS60259179A (ja) * | 1984-06-05 | 1985-12-21 | Teijin Ltd | 細胞培養槽及び細胞培養方法 |
| EP0192705A1 (en) * | 1984-08-28 | 1986-09-03 | Massachusetts Institute Of Technology | Detachment of anchorage-dependent cells from microcarriers |
-
1987
- 1987-11-23 DE DE19873739649 patent/DE3739649A1/de active Granted
-
1988
- 1988-11-03 CA CA000582141A patent/CA1322541C/en not_active Expired - Fee Related
- 1988-11-03 DK DK198806152A patent/DK174998B1/da not_active IP Right Cessation
- 1988-11-04 EP EP88118431A patent/EP0317810B1/de not_active Expired - Lifetime
- 1988-11-04 ES ES88118431T patent/ES2063016T3/es not_active Expired - Lifetime
- 1988-11-04 DE DE3851206T patent/DE3851206D1/de not_active Expired - Fee Related
- 1988-11-04 AT AT88118431T patent/ATE110411T1/de not_active IP Right Cessation
- 1988-11-21 US US07/274,430 patent/US5100799A/en not_active Expired - Lifetime
- 1988-11-22 JP JP63295877A patent/JPH0740929B2/ja not_active Expired - Fee Related
- 1988-11-23 FI FI885435A patent/FI89506C/fi not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| FI89506C (fi) | 1993-10-11 |
| DK615288D0 (da) | 1988-11-03 |
| FI885435A7 (fi) | 1989-05-24 |
| FI885435A0 (fi) | 1988-11-23 |
| DK615288A (da) | 1989-05-24 |
| ATE110411T1 (de) | 1994-09-15 |
| EP0317810A2 (de) | 1989-05-31 |
| US5100799A (en) | 1992-03-31 |
| DE3739649C2 (OSRAM) | 1989-11-02 |
| FI89506B (fi) | 1993-06-30 |
| CA1322541C (en) | 1993-09-28 |
| JPH01168273A (ja) | 1989-07-03 |
| DE3739649A1 (de) | 1989-06-22 |
| DE3851206D1 (de) | 1994-09-29 |
| ES2063016T3 (es) | 1995-01-01 |
| EP0317810A3 (de) | 1991-02-06 |
| EP0317810B1 (de) | 1994-08-24 |
| DK174998B1 (da) | 2004-04-13 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| LAPS | Cancellation because of no payment of annual fees |