DK173445B1 - Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba - Google Patents

Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba Download PDF

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Publication number
DK173445B1
DK173445B1 DK198700097A DK9787A DK173445B1 DK 173445 B1 DK173445 B1 DK 173445B1 DK 198700097 A DK198700097 A DK 198700097A DK 9787 A DK9787 A DK 9787A DK 173445 B1 DK173445 B1 DK 173445B1
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Denmark
Prior art keywords
nuclease
serratia spp
enzyme
serratia
dna
Prior art date
Application number
DK198700097A
Other languages
Danish (da)
English (en)
Other versions
DK9787D0 (da
DK9787A (da
Inventor
Soeren Molin
Michael Givskov
Erik Riise
Original Assignee
Nycomed Danmark As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK210085A external-priority patent/DK210085D0/da
Priority claimed from DK606085A external-priority patent/DK606085D0/da
Application filed by Nycomed Danmark As filed Critical Nycomed Danmark As
Priority to DK198700097A priority Critical patent/DK173445B1/da
Publication of DK9787D0 publication Critical patent/DK9787D0/da
Publication of DK9787A publication Critical patent/DK9787A/da
Application granted granted Critical
Publication of DK173445B1 publication Critical patent/DK173445B1/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Compounds Of Unknown Constitution (AREA)
DK198700097A 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba DK173445B1 (da)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK198700097A DK173445B1 (da) 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
DK210085A DK210085D0 (da) 1985-05-10 1985-05-10 Fremgangsmaade til fremstilling af bakterielle enzymer
DK210085 1985-05-10
DK606085A DK606085D0 (da) 1985-12-23 1985-12-23 Fremgangsmaade til fremstilling af et bakterielt enzym
DK606085 1985-12-23
PCT/DK1986/000051 WO1986006743A1 (en) 1985-05-10 1986-05-09 Bacterial enzymes
DK8600051 1986-05-09
DK9787 1987-01-09
DK198700097A DK173445B1 (da) 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba

Publications (3)

Publication Number Publication Date
DK9787D0 DK9787D0 (da) 1987-01-09
DK9787A DK9787A (da) 1987-01-09
DK173445B1 true DK173445B1 (da) 2000-11-06

Family

ID=26066359

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198700097A DK173445B1 (da) 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba

Country Status (13)

Country Link
EP (1) EP0229866B1 (de)
KR (1) KR930002887B1 (de)
CN (1) CN1008377B (de)
AT (1) ATE83264T1 (de)
AU (2) AU594062B2 (de)
CA (1) CA1327328C (de)
DE (2) DE229866T1 (de)
DK (1) DK173445B1 (de)
ES (2) ES8800346A1 (de)
FI (1) FI870052A0 (de)
IE (1) IE59296B1 (de)
IL (1) IL78703A (de)
WO (1) WO1986006743A1 (de)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3939771A1 (de) * 1989-12-01 1991-06-06 Behringwerke Ag Verfahren zur biologischen inaktivierung von nukleinsaeuren
IL103846A0 (en) * 1991-11-25 1993-04-04 Tanabe Seiyaku Co Gene coding for esterase and novel microorganism containing said gene
US5834233A (en) * 1992-04-06 1998-11-10 Gx Biosystems A/S Method of limiting the survival of genetically engineered microorganisms in their enivronment
CH682541A5 (de) * 1992-06-23 1993-10-15 Urs Viktor Dr Wirth Biochemische Inaktivierung von RNA-Viren.
CA2137728C (en) * 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
US5968777A (en) * 1996-04-18 1999-10-19 Smithkline Beecham Corporation DNA encoding seryl t-RNA synthetase
HUE027196T2 (hu) 2002-09-06 2016-10-28 Genentech Inc Eljárás fehérjeextrakcióra
EP2133418A4 (de) 2007-03-07 2010-04-07 On Chip Cellomics Consortium Zellisolierungsverfahren, zelltestverfahren und reagenskit dafür
EP2108956A1 (de) 2008-04-07 2009-10-14 Erasmus University Medical Center Rotterdam Verbesserte Verfahren und Kits zum Erkennen von tumorspezifischen Fusionsproteinen
DK3192874T3 (da) 2008-06-18 2019-12-16 Oxford Biomedica Ltd Virusoprensning
DK2462224T3 (en) 2009-08-03 2017-09-04 C-Lecta Gmbh PROCEDURE FOR MANUFACTURING NUCLEASES OF A GRAM NEGATIVE BACTERY WHEN USING A GRAM POSITIVE EXPRESSION HOST
JP6141989B2 (ja) 2012-10-12 2017-06-07 セイジ サイエンス，インコーポレイティド 側方溶出型分子分画装置
CN103205448B (zh) * 2013-02-18 2016-03-23 徐东 一种裂解时自动降解核酸的大肠杆菌制备的方法
CN104099310B (zh) * 2013-04-12 2021-06-18 杭州俊丰生物工程有限公司 一种重组核酸酶及其制备方法
US10131901B2 (en) 2014-10-15 2018-11-20 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
US10655122B2 (en) 2015-04-20 2020-05-19 Qiagen Gmbh Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample
CA3005895A1 (en) 2015-11-20 2017-05-26 Washington University Preparative electrophoretic method for targeted purification of genomic dna fragments
WO2017139669A1 (en) * 2016-02-11 2017-08-17 Sage Science, Inc. Workflows for enzymatic nucleic acid modification
EP3607308A1 (de) 2017-04-07 2020-02-12 Sage Science, Inc. Systeme und verfahren zum nachweis genetischer struktureller variationen unter verwendung von integrierter elektrophoretischer dna-reinigung
CN112367973A (zh) 2018-05-15 2021-02-12 旗舰先锋创新V股份有限公司 融合剂脂质体组合物和其用途
CN113613633A (zh) 2018-11-14 2021-11-05 旗舰先锋创新V股份有限公司 用于cns递送的融合剂脂质体组合物
WO2020102485A1 (en) 2018-11-14 2020-05-22 Flagship Pioneering Innovations V, Inc. Fusosome compositions for hematopoietic stem cell delivery
US20230043255A1 (en) 2018-11-14 2023-02-09 Flagship Pioneering Innovations V, Inc. Fusosome compositions for t cell delivery
WO2020129986A1 (ja) * 2018-12-21 2020-06-25 一般財団法人阪大微生物病研究会 ボツリヌス毒素の製造方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5315491A (en) * 1976-07-23 1978-02-13 Rikagaku Kenkyusho Preparation of novel nucleicacidase
US4174400A (en) * 1978-09-13 1979-11-13 Merck & Co., Inc. Anthelmintic benzimidazoles
DE3380262D1 (en) * 1982-05-25 1989-08-31 Lilly Co Eli Cloning vectors for expression of exogenous protein
JPS59183688A (ja) * 1983-03-31 1984-10-18 Tanabe Seiyaku Co Ltd Ｌ−アスパラギン酸の製法
GB2142337A (en) * 1983-07-01 1985-01-16 Celltech Ltd Preparation of lingual lipase protein
DE3334847A1 (de) * 1983-09-27 1985-04-04 Hoechst Ag, 6230 Frankfurt Immobilisierte 5'-phosphodiesterase, verfahren zu ihrer herstellung und ihre verwendung

Also Published As

Publication number Publication date
FI870052A (fi) 1987-01-07
EP0229866A1 (de) 1987-07-29
ATE83264T1 (de) 1992-12-15
KR880700069A (ko) 1988-02-15
DE229866T1 (de) 1988-01-14
KR930002887B1 (ko) 1993-04-12
ES554856A0 (es) 1987-11-01
AU5910086A (en) 1986-12-04
IL78703A (en) 1993-02-21
DK9787D0 (da) 1987-01-09
DE3687266T2 (de) 1993-04-15
WO1986006743A1 (en) 1986-11-20
DK9787A (da) 1987-01-09
IE59296B1 (en) 1994-02-09
ES557687A0 (es) 1988-03-01
EP0229866B1 (de) 1992-12-09
CA1327328C (en) 1994-03-01
ES8801946A1 (es) 1988-03-01
IL78703A0 (en) 1986-08-31
FI870052A0 (fi) 1987-01-07
CN86104387A (zh) 1987-04-22
IE861236L (en) 1986-11-10
AU3679389A (en) 1989-11-23
DE3687266D1 (de) 1993-01-21
CN1008377B (zh) 1990-06-13
AU594062B2 (en) 1990-03-01
ES8800346A1 (es) 1987-11-01

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B1 Patent granted (law 1993)
PUP Patent expired