DK9787A - Bakterielle enzymer - Google Patents

Bakterielle enzymer

Info

Publication number
DK9787A
DK9787A DK009787A DK9787A DK9787A DK 9787 A DK9787 A DK 9787A DK 009787 A DK009787 A DK 009787A DK 9787 A DK9787 A DK 9787A DK 9787 A DK9787 A DK 9787A
Authority
DK
Denmark
Prior art keywords
enzymes
serratia species
nuclease
bacterial enzymes
organism
Prior art date
Application number
DK009787A
Other languages
English (en)
Other versions
DK173445B1 (da
DK9787D0 (da
Inventor
Soeren Molin
Michael Givskov
Erik Riise
Original Assignee
Benzon As Alfred
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK210085A external-priority patent/DK210085D0/da
Priority claimed from DK606085A external-priority patent/DK606085D0/da
Application filed by Benzon As Alfred filed Critical Benzon As Alfred
Priority to DK198700097A priority Critical patent/DK173445B1/da
Publication of DK9787A publication Critical patent/DK9787A/da
Publication of DK9787D0 publication Critical patent/DK9787D0/da
Application granted granted Critical
Publication of DK173445B1 publication Critical patent/DK173445B1/da

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70Vectors or expression systems specially adapted for E. coli
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08Reducing the nucleic acid content
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67General methods for enhancing the expression
    • C12N15/69Increasing the copy number of the vector
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18Carboxylic ester hydrolases (3.1.1)
    • C12N9/20Triglyceride splitting, e.g. by means of lipase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/16Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22Ribonucleases RNAses, DNAses
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K2319/00Fusion polypeptide
    • C07K2319/01Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)
  • Compounds Of Unknown Constitution (AREA)
DK198700097A 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba DK173445B1 (da)

Priority Applications (1)

Application Number Priority Date Filing Date Title
DK198700097A DK173445B1 (da) 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba

Applications Claiming Priority (8)

Application Number Priority Date Filing Date Title
DK210085A DK210085D0 (da) 1985-05-10 1985-05-10 Fremgangsmaade til fremstilling af bakterielle enzymer
DK210085 1985-05-10
DK606085A DK606085D0 (da) 1985-12-23 1985-12-23 Fremgangsmaade til fremstilling af et bakterielt enzym
DK606085 1985-12-23
PCT/DK1986/000051 WO1986006743A1 (en) 1985-05-10 1986-05-09 Bacterial enzymes
DK8600051 1986-05-09
DK9787 1987-01-09
DK198700097A DK173445B1 (da) 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba

Publications (3)

Publication Number Publication Date
DK9787A true DK9787A (da) 1987-01-09
DK9787D0 DK9787D0 (da) 1987-01-09
DK173445B1 DK173445B1 (da) 2000-11-06

Family

ID=26066359

Family Applications (1)

Application Number Title Priority Date Filing Date
DK198700097A DK173445B1 (da) 1985-05-10 1987-01-09 Fremgangsmåde til fremstilling af et enzym fra Serratia spp., hybridplasmid med indsat DNA-fragment fra Serratia spp. og ba

Country Status (13)

Country Link
EP (1) EP0229866B1 (da)
KR (1) KR930002887B1 (da)
CN (1) CN1008377B (da)
AT (1) ATE83264T1 (da)
AU (2) AU594062B2 (da)
CA (1) CA1327328C (da)
DE (2) DE229866T1 (da)
DK (1) DK173445B1 (da)
ES (2) ES8800346A1 (da)
FI (1) FI870052A0 (da)
IE (1) IE59296B1 (da)
IL (1) IL78703A (da)
WO (1) WO1986006743A1 (da)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3939771A1 (de) * 1989-12-01 1991-06-06 Behringwerke Ag Verfahren zur biologischen inaktivierung von nukleinsaeuren
IL103846A0 (en) * 1991-11-25 1993-04-04 Tanabe Seiyaku Co Gene coding for esterase and novel microorganism containing said gene
US5834233A (en) * 1992-04-06 1998-11-10 Gx Biosystems A/S Method of limiting the survival of genetically engineered microorganisms in their enivronment
CH682541A5 (de) * 1992-06-23 1993-10-15 Urs Viktor Dr Wirth Biochemische Inaktivierung von RNA-Viren.
CA2137728C (en) * 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
US5968777A (en) * 1996-04-18 1999-10-19 Smithkline Beecham Corporation DNA encoding seryl t-RNA synthetase
US6967241B2 (en) 2002-09-06 2005-11-22 Genentech, Inc. Process for protein extraction
US20100092969A1 (en) 2007-03-07 2010-04-15 On-Chip Cellomics Consortium Cell isolation method, cell testing method and reagent kit therefor
EP2108956A1 (en) 2008-04-07 2009-10-14 Erasmus University Medical Center Rotterdam Improved methods and kits for detecting tumor-specific fusion proteins
US9169491B2 (en) 2008-06-18 2015-10-27 Oxford Biomedica (Uk) Limited Virus purification
DK2462224T3 (da) 2009-08-03 2017-09-04 C-Lecta Gmbh Fremgangsmåde til fremstilling af nukleaser af et gram negativt bakterie under udnyttelse af en gram positiv ekspressionsvært
AU2013329110B2 (en) 2012-10-12 2017-05-04 Sage Science, Inc. Side-eluting molecular fractionator
CN103205448B (zh) * 2013-02-18 2016-03-23 徐东 一种裂解时自动降解核酸的大肠杆菌制备的方法
CN104099310B (zh) * 2013-04-12 2021-06-18 杭州俊丰生物工程有限公司 一种重组核酸酶及其制备方法
EP3207163B1 (en) 2014-10-15 2020-05-27 Sage Science, Inc. Apparatuses, methods and systems for automated processing of nucleic acids and electrophoretic sample preparation
ES2938752T3 (es) 2015-04-20 2023-04-14 Qiagen Gmbh Método, solución de lisis y kit para la reducción selectiva de ácidos nucleicos animales en una muestra
JP7054678B2 (ja) 2015-11-20 2022-04-14 ワシントン・ユニバーシティ ゲノムdna断片の標的化された精製のための調製用電気泳動方法
WO2017139669A1 (en) * 2016-02-11 2017-08-17 Sage Science, Inc. Workflows for enzymatic nucleic acid modification
CA3055306A1 (en) 2017-04-07 2018-10-11 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic dna purification
WO2019222403A2 (en) 2018-05-15 2019-11-21 Flagship Pioneering Innovations V, Inc. Fusosome compositions and uses thereof
JP2022507454A (ja) 2018-11-14 2022-01-18 フラッグシップ パイオニアリング イノベーションズ ブイ, インコーポレイテッド Cns送達のためのフソソーム組成物
WO2020102485A1 (en) 2018-11-14 2020-05-22 Flagship Pioneering Innovations V, Inc. Fusosome compositions for hematopoietic stem cell delivery
JP2022507453A (ja) 2018-11-14 2022-01-18 フラッグシップ パイオニアリング イノベーションズ ブイ, インコーポレイテッド T細胞送達のためのフソソーム組成物
EP3901163A4 (en) * 2018-12-21 2022-10-05 The Research Foundation for Microbial Diseases of Osaka University PROCESS FOR THE PRODUCTION OF BOTULINUM TOXIN

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5315491A (en) * 1976-07-23 1978-02-13 Rikagaku Kenkyusho Preparation of novel nucleicacidase
US4174400A (en) * 1978-09-13 1979-11-13 Merck & Co., Inc. Anthelmintic benzimidazoles
GB2121054B (en) * 1982-05-25 1986-02-26 Lilly Co Eli Cloning vectors for expression of exogenous protein
JPS59183688A (ja) * 1983-03-31 1984-10-18 Tanabe Seiyaku Co Ltd L−アスパラギン酸の製法
WO1985000381A1 (en) * 1983-07-01 1985-01-31 Celltech Limited Proteins, pharmaceutical compositions, genes, vectors, host organisms and processes for their production
DE3334847A1 (de) * 1983-09-27 1985-04-04 Hoechst Ag, 6230 Frankfurt Immobilisierte 5'-phosphodiesterase, verfahren zu ihrer herstellung und ihre verwendung

Also Published As

Publication number Publication date
ATE83264T1 (de) 1992-12-15
ES8801946A1 (es) 1988-03-01
CN1008377B (zh) 1990-06-13
IL78703A0 (en) 1986-08-31
ES557687A0 (es) 1988-03-01
DE229866T1 (de) 1988-01-14
DE3687266T2 (de) 1993-04-15
ES8800346A1 (es) 1987-11-01
IL78703A (en) 1993-02-21
AU594062B2 (en) 1990-03-01
EP0229866A1 (en) 1987-07-29
IE59296B1 (en) 1994-02-09
WO1986006743A1 (en) 1986-11-20
FI870052A (fi) 1987-01-07
ES554856A0 (es) 1987-11-01
AU5910086A (en) 1986-12-04
CN86104387A (zh) 1987-04-22
EP0229866B1 (en) 1992-12-09
FI870052A0 (fi) 1987-01-07
IE861236L (en) 1986-11-10
KR880700069A (ko) 1988-02-15
CA1327328C (en) 1994-03-01
DK173445B1 (da) 2000-11-06
KR930002887B1 (ko) 1993-04-12
DK9787D0 (da) 1987-01-09
AU3679389A (en) 1989-11-23
DE3687266D1 (de) 1993-01-21

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Legal Events

Date Code Title Description
B1 Patent granted (law 1993)
PUP Patent expired