ES8800346A1 - Un metodo de produccion de una enzima bacteriana - Google Patents

Un metodo de produccion de una enzima bacteriana

Info

Publication number
ES8800346A1
ES8800346A1 ES554856A ES554856A ES8800346A1 ES 8800346 A1 ES8800346 A1 ES 8800346A1 ES 554856 A ES554856 A ES 554856A ES 554856 A ES554856 A ES 554856A ES 8800346 A1 ES8800346 A1 ES 8800346A1
Authority
ES
Spain
Prior art keywords
enzymes
serratia spp
production
nuclease
employed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES554856A
Other languages
English (en)
Other versions
ES554856A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Alfred Benzon AS
Original Assignee
Alfred Benzon AS
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK210085A external-priority patent/DK210085D0/da
Priority claimed from DK606085A external-priority patent/DK606085D0/da
Application filed by Alfred Benzon AS filed Critical Alfred Benzon AS
Publication of ES554856A0 publication Critical patent/ES554856A0/es
Publication of ES8800346A1 publication Critical patent/ES8800346A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Saccharide Compounds (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

METODO PARA PRODUCIR UNA ENZIMA BACTERIANA. COMPRENDE: A) DIGERIR UN PLASMIDO DE PARTIDA CON UNA NUCLEASA DE RESTRICCION, PARA PRODUCIR UN PLASMIDO LINEALIZADO; B) INCUBAR EL PLASMIDO LINEALIZADO CON UN FRAGMENTO DE ADN PROCEDENTE DE SERRATIA SPP QUE CODIFICA UNA ENZINA DE SERRATIA SPP; C) LIGAR EL CONJUNTO DE, PARA RECIRCULARIZAR EL PLASMIDO Y PRODUCIR UN PLASMIDO HIBRIDO CAPAZ DE EXPRESAR LA ENZIMA EXTRACELULAR, D) TRATAR EL PLASMIDO HIBRIDO CON UN MICROORGANISMO HUESPED; E) CULTIVAR EL MICROORGANISMO HUESPED EN UN MEDIO LIQUIDO CON NUTRIENTES Y MINERALES ADECUADOS Y DURANTE UN PERIODO DE TIEMPO ADECUADO, PARA ASEGURAR LA EXPRESION DE LA ENZIMA EXTRACELULAR; F) FILTRAR EL MEDIO DE CULTIO, PARA RECOGER EL ENZIMA EXTRACELULAR EXCRETADO POR LAS CELULAS DEL MEDIO DE CULTIVO; G) PLURIFICAR LA ENZIMA EXTRACELULAR POR CROMATOGRAFIA. LA ENZIMA DE SERRATIA SPP EN UNA ENZIMA HIDROFILICA Y EL MICROORGANISMO EN UNA BACTERIA GRAM-(-).
ES554856A 1985-05-10 1986-05-09 Un metodo de produccion de una enzima bacteriana Expired ES8800346A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK210085A DK210085D0 (da) 1985-05-10 1985-05-10 Fremgangsmaade til fremstilling af bakterielle enzymer
DK606085A DK606085D0 (da) 1985-12-23 1985-12-23 Fremgangsmaade til fremstilling af et bakterielt enzym

Publications (2)

Publication Number Publication Date
ES554856A0 ES554856A0 (es) 1987-11-01
ES8800346A1 true ES8800346A1 (es) 1987-11-01

Family

ID=26066359

Family Applications (2)

Application Number Title Priority Date Filing Date
ES554856A Expired ES8800346A1 (es) 1985-05-10 1986-05-09 Un metodo de produccion de una enzima bacteriana
ES557687A Expired ES8801946A1 (es) 1985-05-10 1987-08-26 Un metodo de separacion de acidos nucleicos de un material biologico

Family Applications After (1)

Application Number Title Priority Date Filing Date
ES557687A Expired ES8801946A1 (es) 1985-05-10 1987-08-26 Un metodo de separacion de acidos nucleicos de un material biologico

Country Status (13)

Country Link
EP (1) EP0229866B1 (es)
KR (1) KR930002887B1 (es)
CN (1) CN1008377B (es)
AT (1) ATE83264T1 (es)
AU (2) AU594062B2 (es)
CA (1) CA1327328C (es)
DE (2) DE3687266T2 (es)
DK (1) DK173445B1 (es)
ES (2) ES8800346A1 (es)
FI (1) FI870052A0 (es)
IE (1) IE59296B1 (es)
IL (1) IL78703A (es)
WO (1) WO1986006743A1 (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3939771A1 (de) * 1989-12-01 1991-06-06 Behringwerke Ag Verfahren zur biologischen inaktivierung von nukleinsaeuren
IL103846A0 (en) * 1991-11-25 1993-04-04 Tanabe Seiyaku Co Gene coding for esterase and novel microorganism containing said gene
US5834233A (en) * 1992-04-06 1998-11-10 Gx Biosystems A/S Method of limiting the survival of genetically engineered microorganisms in their enivronment
CH682541A5 (de) * 1992-06-23 1993-10-15 Urs Viktor Dr Wirth Biochemische Inaktivierung von RNA-Viren.
CA2137728C (en) * 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
US5968777A (en) * 1996-04-18 1999-10-19 Smithkline Beecham Corporation DNA encoding seryl t-RNA synthetase
DK2292637T3 (en) 2002-09-06 2016-04-04 Genentech Inc Process for protein extraction
US20100092969A1 (en) 2007-03-07 2010-04-15 On-Chip Cellomics Consortium Cell isolation method, cell testing method and reagent kit therefor
EP2108956A1 (en) 2008-04-07 2009-10-14 Erasmus University Medical Center Rotterdam Improved methods and kits for detecting tumor-specific fusion proteins
EP3192874B1 (en) 2008-06-18 2019-10-16 Oxford BioMedica (UK) Limited Virus purification
DK2462224T3 (en) 2009-08-03 2017-09-04 C-Lecta Gmbh PROCEDURE FOR MANUFACTURING NUCLEASES OF A GRAM NEGATIVE BACTERY WHEN USING A GRAM POSITIVE EXPRESSION HOST
JP6141989B2 (ja) 2012-10-12 2017-06-07 セイジ サイエンス，インコーポレイティド 側方溶出型分子分画装置
CN103205448B (zh) * 2013-02-18 2016-03-23 徐东 一种裂解时自动降解核酸的大肠杆菌制备的方法
CN104099310B (zh) * 2013-04-12 2021-06-18 杭州俊丰生物工程有限公司 一种重组核酸酶及其制备方法
CN107109487B (zh) 2014-10-15 2022-03-04 赛琪科学股份有限公司 自动处理核酸和电泳样品制备的装置、方法和系统
WO2016169579A1 (en) 2015-04-20 2016-10-27 Qiagen Gmbh Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample
JP7054678B2 (ja) 2015-11-20 2022-04-14 ワシントン・ユニバーシティ ゲノムｄｎａ断片の標的化された精製のための調製用電気泳動方法
WO2017139669A1 (en) * 2016-02-11 2017-08-17 Sage Science, Inc. Workflows for enzymatic nucleic acid modification
CA3055306A1 (en) 2017-04-07 2018-10-11 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic dna purification
US20210228627A1 (en) 2018-05-15 2021-07-29 Flagship Pioneering Innovations V, Inc. Fusosome compositions and uses thereof
CA3120282A1 (en) 2018-11-14 2020-05-22 Flagship Pioneering Innovations V, Inc. Fusosome compositions for hematopoietic stem cell delivery
EP3880179A2 (en) 2018-11-14 2021-09-22 Flagship Pioneering Innovations V, Inc. Fusosome compositions for cns delivery
JP2022507453A (ja) 2018-11-14 2022-01-18 フラッグシップ パイオニアリング イノベーションズ ブイ， インコーポレイテッド Ｔ細胞送達のためのフソソーム組成物
JP7454507B2 (ja) 2018-12-21 2024-03-22 一般財団法人阪大微生物病研究会 ボツリヌス毒素の製造方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5315491A (en) * 1976-07-23 1978-02-13 Rikagaku Kenkyusho Preparation of novel nucleicacidase
US4174400A (en) * 1978-09-13 1979-11-13 Merck & Co., Inc. Anthelmintic benzimidazoles
GB2121054B (en) * 1982-05-25 1986-02-26 Lilly Co Eli Cloning vectors for expression of exogenous protein
JPS59183688A (ja) * 1983-03-31 1984-10-18 Tanabe Seiyaku Co Ltd Ｌ−アスパラギン酸の製法
WO1985000381A1 (en) * 1983-07-01 1985-01-31 Celltech Limited Proteins, pharmaceutical compositions, genes, vectors, host organisms and processes for their production
DE3334847A1 (de) * 1983-09-27 1985-04-04 Hoechst Ag, 6230 Frankfurt Immobilisierte 5'-phosphodiesterase, verfahren zu ihrer herstellung und ihre verwendung

Also Published As

Publication number Publication date
DK173445B1 (da) 2000-11-06
CN1008377B (zh) 1990-06-13
IL78703A (en) 1993-02-21
IE59296B1 (en) 1994-02-09
FI870052A (fi) 1987-01-07
DK9787A (da) 1987-01-09
DE3687266D1 (de) 1993-01-21
ES557687A0 (es) 1988-03-01
CA1327328C (en) 1994-03-01
IE861236L (en) 1986-11-10
CN86104387A (zh) 1987-04-22
AU5910086A (en) 1986-12-04
ATE83264T1 (de) 1992-12-15
DE3687266T2 (de) 1993-04-15
EP0229866B1 (en) 1992-12-09
AU594062B2 (en) 1990-03-01
IL78703A0 (en) 1986-08-31
EP0229866A1 (en) 1987-07-29
WO1986006743A1 (en) 1986-11-20
AU3679389A (en) 1989-11-23
DE229866T1 (de) 1988-01-14
ES554856A0 (es) 1987-11-01
ES8801946A1 (es) 1988-03-01
FI870052A0 (fi) 1987-01-07
KR880700069A (ko) 1988-02-15
KR930002887B1 (ko) 1993-04-12
DK9787D0 (da) 1987-01-09

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Legal Events

Date Code Title Description
PC1A Transfer granted
FD1A Patent lapsed

Effective date: 20070910