ES8801946A1 - Un metodo de separacion de acidos nucleicos de un material biologico - Google Patents

Un metodo de separacion de acidos nucleicos de un material biologico

Info

Publication number
ES8801946A1
ES8801946A1 ES557687A ES557687A ES8801946A1 ES 8801946 A1 ES8801946 A1 ES 8801946A1 ES 557687 A ES557687 A ES 557687A ES 557687 A ES557687 A ES 557687A ES 8801946 A1 ES8801946 A1 ES 8801946A1
Authority
ES
Spain
Prior art keywords
enzymes
serratia spp
production
nuclease
employed
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired
Application number
ES557687A
Other languages
English (en)
Other versions
ES557687A0 (es
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BENSON AS ALFRED
Original Assignee
BENSON AS ALFRED
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DK210085A external-priority patent/DK210085D0/da
Priority claimed from DK606085A external-priority patent/DK606085D0/da
Application filed by BENSON AS ALFRED filed Critical BENSON AS ALFRED
Publication of ES557687A0 publication Critical patent/ES557687A0/es
Publication of ES8801946A1 publication Critical patent/ES8801946A1/es
Expired legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/08—Reducing the nucleic acid content
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/18—Carboxylic ester hydrolases (3.1.1)
    • C12N9/20—Triglyceride splitting, e.g. by means of lipase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/036—Fusion polypeptide containing a localisation/targetting motif targeting to the medium outside of the cell, e.g. type III secretion

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Jellies, Jams, And Syrups (AREA)
  • Compounds Of Unknown Constitution (AREA)
  • Bakery Products And Manufacturing Methods Therefor (AREA)

Abstract

METODO DE SEPARACION DE ACIDOS NUCLEICOS DE PRODUCTOS OBTENIDOS POR FERMENTACION DE MICROORGANISMOS. CONSISTENTE EN AGREGAR UNA NUCLEASA DE SERRATIA SPP. A DICHO MATERIAL BIOSINTETICO ANTES DE LA LISIS CELULAR E INCUBAR HASTA CONSEGUIR UNA REDUCCION DE LA VISCOSIDAD QUE PERMITE UN PROCESADO POR FILTRACION, CENTRIFUGACION O CROMATOGRAFIA. COMPRENDE TAMBIEN LA SEPARACION DE ACIDOS NUCLEICOS RESIDUALES, POR ADICION AL MATERIAL BIOSINTETICO DE UN DETERGENTE Y/O UN AGENTE CAOTROPICO JUNTO CON LA NUCLEASA. DE APLICACION EN LA PURIFICACION DE PROTEINAS PREPARADAS POR FERMENTACION, EN LA ELIMINACION DE POTENCIAL INFECCIOSO DE AGENTES INFECCIOSOS PARA LA PRODUCCION DE ANTIGENOS, VACUNAS O AGENTES DE DIAGNOSTICO. APLICABLE COMO MEDIDA RESIDUAL RESULTANTE DE LA PRODUCCION INDUSTRIAL DE PRODUCTOS BIOSINTETICOS POR TECNICAS DE ADN RECOMBINANTE.
ES557687A 1985-05-10 1987-08-26 Un metodo de separacion de acidos nucleicos de un material biologico Expired ES8801946A1 (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DK210085A DK210085D0 (da) 1985-05-10 1985-05-10 Fremgangsmaade til fremstilling af bakterielle enzymer
DK606085A DK606085D0 (da) 1985-12-23 1985-12-23 Fremgangsmaade til fremstilling af et bakterielt enzym

Publications (2)

Publication Number Publication Date
ES557687A0 ES557687A0 (es) 1988-03-01
ES8801946A1 true ES8801946A1 (es) 1988-03-01

Family

ID=26066359

Family Applications (2)

Application Number Title Priority Date Filing Date
ES554856A Expired ES8800346A1 (es) 1985-05-10 1986-05-09 Un metodo de produccion de una enzima bacteriana
ES557687A Expired ES8801946A1 (es) 1985-05-10 1987-08-26 Un metodo de separacion de acidos nucleicos de un material biologico

Family Applications Before (1)

Application Number Title Priority Date Filing Date
ES554856A Expired ES8800346A1 (es) 1985-05-10 1986-05-09 Un metodo de produccion de una enzima bacteriana

Country Status (13)

Country Link
EP (1) EP0229866B1 (es)
KR (1) KR930002887B1 (es)
CN (1) CN1008377B (es)
AT (1) ATE83264T1 (es)
AU (2) AU594062B2 (es)
CA (1) CA1327328C (es)
DE (2) DE3687266T2 (es)
DK (1) DK173445B1 (es)
ES (2) ES8800346A1 (es)
FI (1) FI870052A0 (es)
IE (1) IE59296B1 (es)
IL (1) IL78703A (es)
WO (1) WO1986006743A1 (es)

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE3939771A1 (de) * 1989-12-01 1991-06-06 Behringwerke Ag Verfahren zur biologischen inaktivierung von nukleinsaeuren
IL103846A0 (en) * 1991-11-25 1993-04-04 Tanabe Seiyaku Co Gene coding for esterase and novel microorganism containing said gene
US5834233A (en) * 1992-04-06 1998-11-10 Gx Biosystems A/S Method of limiting the survival of genetically engineered microorganisms in their enivronment
CH682541A5 (de) * 1992-06-23 1993-10-15 Urs Viktor Dr Wirth Biochemische Inaktivierung von RNA-Viren.
CA2137728C (en) * 1993-12-14 1998-07-14 William E. Keating Enzymatic removal of non-target nucleic acid from biological samples
US5968777A (en) * 1996-04-18 1999-10-19 Smithkline Beecham Corporation DNA encoding seryl t-RNA synthetase
SI2292637T1 (sl) 2002-09-06 2016-04-29 Genentech, Inc. Postopek za proteinsko ekstrakcijo
EP2133418A4 (en) 2007-03-07 2010-04-07 On Chip Cellomics Consortium METHOD FOR CELL ISOLATION, CELL TEST METHOD, AND REAGENT KIT FOR ITS IMPLEMENTATION
EP2108956A1 (en) 2008-04-07 2009-10-14 Erasmus University Medical Center Rotterdam Improved methods and kits for detecting tumor-specific fusion proteins
EP3192874B1 (en) 2008-06-18 2019-10-16 Oxford BioMedica (UK) Limited Virus purification
WO2011015327A1 (de) 2009-08-03 2011-02-10 C-Lecta Gmbh Verfahren zur herstellung von nukleasen eines gram-negativen bakteriums unter nutzung eines gram-positiven expressionswirtes
JP6141989B2 (ja) 2012-10-12 2017-06-07 セイジ サイエンス，インコーポレイティド 側方溶出型分子分画装置
CN103205448B (zh) * 2013-02-18 2016-03-23 徐东 一种裂解时自动降解核酸的大肠杆菌制备的方法
CN104099310B (zh) * 2013-04-12 2021-06-18 杭州俊丰生物工程有限公司 一种重组核酸酶及其制备方法
JP6612340B2 (ja) 2014-10-15 2019-11-27 セージ サイエンス， インコーポレイテッド 核酸の自動化された加工処理および電気泳動による試料調製のための装置、方法およびシステム
EP3286324B1 (en) 2015-04-20 2020-05-20 Qiagen GmbH Method, lysis solution and kit for selectively depleting animal nucleic acids in a sample
US11542495B2 (en) 2015-11-20 2023-01-03 Sage Science, Inc. Preparative electrophoretic method for targeted purification of genomic DNA fragments
WO2017139669A1 (en) * 2016-02-11 2017-08-17 Sage Science, Inc. Workflows for enzymatic nucleic acid modification
WO2018187779A1 (en) 2017-04-07 2018-10-11 Sage Science, Inc. Systems and methods for detection of genetic structural variation using integrated electrophoretic dna purification
SG11202011015QA (en) 2018-05-15 2020-12-30 Flagship Pioneering Innovations V Inc Fusosome compositions and uses thereof
EP3880179A2 (en) 2018-11-14 2021-09-22 Flagship Pioneering Innovations V, Inc. Fusosome compositions for cns delivery
WO2020102503A2 (en) 2018-11-14 2020-05-22 Flagship Pioneering Innovations V, Inc. Fusosome compositions for t cell delivery
AU2019380517A1 (en) 2018-11-14 2021-06-03 Flagship Pioneering Innovations V, Inc. Fusosome compositions for hematopoietic stem cell delivery
JP7454507B2 (ja) 2018-12-21 2024-03-22 一般財団法人阪大微生物病研究会 ボツリヌス毒素の製造方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5315491A (en) * 1976-07-23 1978-02-13 Rikagaku Kenkyusho Preparation of novel nucleicacidase
US4174400A (en) * 1978-09-13 1979-11-13 Merck & Co., Inc. Anthelmintic benzimidazoles
EP0095361B1 (en) * 1982-05-25 1989-07-26 Eli Lilly And Company Cloning vectors for expression of exogenous protein
JPS59183688A (ja) * 1983-03-31 1984-10-18 Tanabe Seiyaku Co Ltd Ｌ−アスパラギン酸の製法
AU3107684A (en) * 1983-07-01 1985-02-07 Celltech Limited Proteins, pharmaceutical compositions, genes, vectors, host organisms and processes for their production
DE3334847A1 (de) * 1983-09-27 1985-04-04 Hoechst Ag, 6230 Frankfurt Immobilisierte 5'-phosphodiesterase, verfahren zu ihrer herstellung und ihre verwendung

Also Published As

Publication number Publication date
IL78703A0 (en) 1986-08-31
FI870052A (fi) 1987-01-07
DK9787D0 (da) 1987-01-09
FI870052A0 (fi) 1987-01-07
ES557687A0 (es) 1988-03-01
DK173445B1 (da) 2000-11-06
IE59296B1 (en) 1994-02-09
KR930002887B1 (ko) 1993-04-12
ES554856A0 (es) 1987-11-01
CN86104387A (zh) 1987-04-22
DE3687266T2 (de) 1993-04-15
IL78703A (en) 1993-02-21
ES8800346A1 (es) 1987-11-01
DK9787A (da) 1987-01-09
CA1327328C (en) 1994-03-01
AU5910086A (en) 1986-12-04
WO1986006743A1 (en) 1986-11-20
AU3679389A (en) 1989-11-23
ATE83264T1 (de) 1992-12-15
AU594062B2 (en) 1990-03-01
DE229866T1 (de) 1988-01-14
IE861236L (en) 1986-11-10
CN1008377B (zh) 1990-06-13
EP0229866A1 (en) 1987-07-29
DE3687266D1 (de) 1993-01-21
EP0229866B1 (en) 1992-12-09
KR880700069A (ko) 1988-02-15

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PC1A Transfer granted
FD1A Patent lapsed

Effective date: 20080112