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RIKEN Institute of Physical and Chemical Research
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RIKEN Institute of Physical and Chemical Research
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Application filed by RIKEN Institute of Physical and Chemical ResearchfiledCriticalRIKEN Institute of Physical and Chemical Research
Priority to JP6834680ApriorityCriticalpatent/JPS585680B2/en
Publication of JPS56164798ApublicationCriticalpatent/JPS56164798A/en
Publication of JPS585680B2publicationCriticalpatent/JPS585680B2/en
PURPOSE: A physiologically active substance that links with the substrate of deoxyribonucleic acid (DNA) is detected by adding a DNA-relaxig enzyme or single-chain DNase to the culture mixture obtained by the cultivation of a bacterial strain producing the above physilologically active substance to effect the reaction.
CONSTITUTION: In the detection of a physiologically active substance linking with DNA such as actionmycin, a bacterium producing actinomycin is cultured, the culture mixture is filtered and heat treated. The product is combined with DNA and a DNA-relaxing enzyme such as HG14385 or single-chain DNase such as nuclease S1 to effect the enzymatic reaction. Then, the reaction mixture is extracted and the aqueous layer is subjected to the electrophoresis with agarose tube gel to detect whether the twisting of DNA is relaxed to form single-chain DNA or DNA broken into linear chains or not. The presence of the physiologically active substance inhibits the relaxation of the twisting of DNA and linear breakage and the substance is detected and determined.
COPYRIGHT: (C)1981,JPO&Japio
JP6834680A1980-05-221980-05-22
New detection method for physiologically active substances
ExpiredJPS585680B2
(en)
Fragment of isolated dna, encoding delta-6-desaturase of cyanobacteria (variants), vector, method for obtaining plants with increased content of gamma-linolenic acid (variants), method for inducing production octadecatetraeonic acid (variants) and cyanobacterial delta-6-desaturase