DK1375664T3 - Fremgangsmåde til udskillelsen og fremstilling af protein - Google Patents

Fremgangsmåde til udskillelsen og fremstilling af protein

Info

Publication number
DK1375664T3
DK1375664T3 DK02708684.2T DK02708684T DK1375664T3 DK 1375664 T3 DK1375664 T3 DK 1375664T3 DK 02708684 T DK02708684 T DK 02708684T DK 1375664 T3 DK1375664 T3 DK 1375664T3
Authority
DK
Denmark
Prior art keywords
coryneform bacterium
heterologous protein
protein
production
sequence encoding
Prior art date
Application number
DK02708684.2T
Other languages
English (en)
Inventor
Yoshimi Kikuchi
Masayo Date
Yukiko Umezawa
Keiichi Yokoyama
Haruo Heima
Hiroshi Matsui
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Application granted granted Critical
Publication of DK1375664T3 publication Critical patent/DK1375664T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1016—Tetrapeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Peptides Or Proteins (AREA)
DK02708684.2T 2001-03-30 2002-03-27 Fremgangsmåde til udskillelsen og fremstilling af protein DK1375664T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001098808 2001-03-30
PCT/JP2002/002978 WO2002081694A1 (fr) 2001-03-30 2002-03-27 Procede de secretion et de production de proteine

Publications (1)

Publication Number Publication Date
DK1375664T3 true DK1375664T3 (da) 2010-02-08

Family

ID=18952423

Family Applications (1)

Application Number Title Priority Date Filing Date
DK02708684.2T DK1375664T3 (da) 2001-03-30 2002-03-27 Fremgangsmåde til udskillelsen og fremstilling af protein

Country Status (14)

Country Link
US (1) US7252972B2 (da)
EP (1) EP1375664B1 (da)
JP (1) JP4362651B2 (da)
KR (1) KR100602807B1 (da)
CN (1) CN1250722C (da)
AT (1) ATE447615T1 (da)
AU (1) AU2002242986A1 (da)
BR (1) BR0208136B1 (da)
CA (1) CA2442679C (da)
DE (1) DE60234247D1 (da)
DK (1) DK1375664T3 (da)
ES (1) ES2335750T3 (da)
RU (1) RU2264463C2 (da)
WO (1) WO2002081694A1 (da)

Families Citing this family (42)

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HU229834B1 (en) 2000-01-21 2014-09-29 Ajinomoto Kk Process for producing l-lysine
RU2316594C2 (ru) * 2003-03-07 2008-02-10 Адзиномото Ко., Инк. Способ продуцирования микробной трансглутаминазы
DE10359594A1 (de) * 2003-12-18 2005-07-28 Basf Ag PEF-TU-Expressionseinheiten
DE10359660A1 (de) * 2003-12-18 2005-07-28 Basf Ag Psod-Expressionseinheiten
EP1748077B1 (en) * 2004-04-20 2013-02-27 Ajinomoto Co., Inc. Method of producing protein
US7527947B2 (en) 2004-06-14 2009-05-05 Novozymes A/S Signal peptide for producing a polypeptide
WO2005121333A1 (en) * 2004-06-14 2005-12-22 Novozymes A/S Signal peptide for producing a polypeptide
DE102004035065A1 (de) 2004-07-20 2006-02-16 Basf Ag P-ET-TS-Expressionseinheiten
KR100620092B1 (ko) * 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
EP1902126A1 (en) * 2005-07-12 2008-03-26 Codon Devices, Inc Compositions and methods for biocatalytic engineering
RU2338784C2 (ru) * 2006-03-24 2008-11-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Новая альдолаза, днк, кодирующая альдолазу, клетки, трансформированные днк, способ получения альдолазы и способ получения 4-гидрокси-l-изолейцина (варианты)
EP2082045B1 (en) * 2006-10-24 2015-03-18 Basf Se Method of reducing gene expression using modified codon usage
AU2008215422B2 (en) * 2007-02-15 2011-07-14 Ajinomoto Co., Inc. Transglutaminase having disulfide bond introduced therein
JP5093228B2 (ja) 2007-03-29 2012-12-12 味の素株式会社 接着用酵素製剤及び接着成形食品の製造方法
KR20100085083A (ko) 2007-11-19 2010-07-28 아지노모토 가부시키가이샤 섬유 가공물 및 이의 제조법
EP2302066A4 (en) * 2008-07-09 2012-02-08 Ajinomoto Kk METHOD FOR PRODUCING AMINOHYDROXYBENZOIC ACID
TWI444146B (zh) 2008-09-25 2014-07-11 Ajinomoto Kk 黏著成型食品用酵素製劑及黏著成型食品之製造方法
WO2010101256A1 (ja) 2009-03-06 2010-09-10 味の素株式会社 放線菌由来の耐熱性トランスグルタミナーゼ
US8871477B2 (en) 2010-02-23 2014-10-28 Toray Industries, Inc. Process for production of cadaverine
EP2772546B1 (en) * 2011-10-25 2019-05-15 Ajinomoto Co., Inc. Secretion production method for protein
IN2014CN04002A (da) 2011-11-02 2015-10-23 Ajinomoto Kk
CN103946371B (zh) 2011-11-02 2016-07-06 味之素株式会社 用于分泌产生蛋白质的方法
SG10201604464SA (en) 2012-03-26 2016-07-28 Axcella Health Inc Charged nutritive proteins and methods
EP3715365A1 (en) 2012-03-26 2020-09-30 Axcella Health Inc. Nutritive fragments, proteins and methods
US9605040B2 (en) 2012-03-26 2017-03-28 Axcella Health Inc. Nutritive proteins and methods
CA2868469A1 (en) 2012-03-26 2013-10-03 Pronutria, Inc. Nutritive fragments, proteins and methods
KR20160058940A (ko) 2013-09-25 2016-05-25 프로뉴트리아 바이오사이언시스, 인코퍼레이티드 근육량, 강도 및 성능을 유지하기 위한 조성물 및 제형, 그리고 이의 생산방법 및 용도
CN107532163A (zh) 2015-04-24 2018-01-02 味之素株式会社 蛋白质的分泌生产方法
EP3375883B1 (en) 2015-11-12 2021-02-03 Ajinomoto Co., Inc. Method for producing n epsilon -acyl-l-lysine
EP3453758A4 (en) 2016-05-02 2019-12-04 Ajinomoto Co., Inc. FC PROTEIN WITH AZID GROUP
DK3533875T5 (da) * 2016-09-01 2024-09-02 Ningxia Eppen Biotech Co Ltd Corynebacterium til fremstilling af l-lysin ved fermentering
EP3530748B1 (en) 2016-10-21 2021-06-02 Ajinomoto Co., Inc. Method for the secretory production proteins
CN109937258B (zh) 2016-10-21 2023-06-23 味之素株式会社 蛋白质的分泌产生方法
JP7088026B2 (ja) 2017-01-19 2022-06-21 味の素株式会社 ヘキスロン酸残基が異性化されたヘパロサン化合物の製造方法
EP3415622A1 (en) 2017-06-14 2018-12-19 Evonik Degussa GmbH Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases
CN117417912A (zh) 2017-09-05 2024-01-19 味之素株式会社 2-o-硫酸化酶突变体和3-o-硫酸化酶突变体及其使用方法
KR20210002575A (ko) 2018-04-20 2021-01-08 아지노모토 가부시키가이샤 단백질의 분비 생산법
WO2020085511A1 (ja) 2018-10-25 2020-04-30 味の素株式会社 タンパク質の分泌生産法
JPWO2020090979A1 (ja) 2018-10-31 2021-09-24 味の素株式会社 抗体に対する親和性物質、切断性部分および反応性基を有する化合物またはその塩
EP4115740A4 (en) 2020-03-04 2024-04-03 Ajinomoto Co., Inc. MUTANT TRANSGLUTAMINASE
BR112023005635A2 (pt) 2020-09-29 2023-04-25 Ajinomoto Kk Método e composição para produzir um alimento, transglutaminase mutante, gene, vetor, e, microrganismo
JPWO2023282315A1 (da) 2021-07-07 2023-01-12

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DE3584848D1 (de) * 1984-04-04 1992-01-23 Ajinomoto Kk Zusammengesetztes transduzierbares plasmid.
JPH07112431B2 (ja) * 1985-09-06 1995-12-06 味の素株式会社 遺伝子発現調節法
US4965197A (en) * 1987-06-12 1990-10-23 Massachusetts Institute Of Technology Coryneform expression and secretion system
WO1988009821A1 (en) * 1987-06-12 1988-12-15 Massachusetts Institute Of Technology Coryneform expression and secretion system
EP0551506A1 (fr) * 1991-07-30 1993-07-21 Orsan Systeme d'expression et de secretion de proteines utilisables en particulier chez les corynebacteries
JP3541385B2 (ja) * 1993-01-13 2004-07-07 味の素株式会社 新規細胞表層タンパク質
JP3711658B2 (ja) 1996-10-07 2005-11-02 味の素株式会社 コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質
PT897003E (pt) * 1997-07-05 2004-09-30 Nestle Sa Expressao aumentada de enzimas proteoliticas em fermento koji
JPH11169182A (ja) * 1997-12-10 1999-06-29 Mitsubishi Chemical Corp コリネ型細菌内で蛋白質を効率よく培地中へ分泌させる変異型分泌装置遺伝子
EP1219713B1 (en) * 1999-09-30 2011-11-09 Ajinomoto Co., Inc. Process for producing transglutaminase

Also Published As

Publication number Publication date
CA2442679C (en) 2010-06-01
BR0208136B1 (pt) 2014-10-21
BR0208136A (pt) 2004-03-02
KR100602807B1 (ko) 2006-07-20
CN1250722C (zh) 2006-04-12
WO2002081694A8 (fr) 2002-11-14
AU2002242986A1 (en) 2002-10-21
RU2003128977A (ru) 2005-02-27
EP1375664B1 (en) 2009-11-04
JPWO2002081694A1 (ja) 2004-07-29
US20040126847A1 (en) 2004-07-01
RU2264463C2 (ru) 2005-11-20
ATE447615T1 (de) 2009-11-15
JP4362651B2 (ja) 2009-11-11
CA2442679A1 (en) 2002-10-17
CN1500145A (zh) 2004-05-26
EP1375664A1 (en) 2004-01-02
KR20030087042A (ko) 2003-11-12
US7252972B2 (en) 2007-08-07
DE60234247D1 (de) 2009-12-17
ES2335750T3 (es) 2010-04-05
EP1375664A4 (en) 2005-08-17
WO2002081694A1 (fr) 2002-10-17

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