ATE447615T1 - Verfahren zur sekretion und produktion von protein - Google Patents

Verfahren zur sekretion und produktion von protein

Info

Publication number
ATE447615T1
ATE447615T1 AT02708684T AT02708684T ATE447615T1 AT E447615 T1 ATE447615 T1 AT E447615T1 AT 02708684 T AT02708684 T AT 02708684T AT 02708684 T AT02708684 T AT 02708684T AT E447615 T1 ATE447615 T1 AT E447615T1
Authority
AT
Austria
Prior art keywords
coryneform bacterium
heterologous protein
protein
production
sequence encoding
Prior art date
Application number
AT02708684T
Other languages
English (en)
Inventor
Yoshimi Kikuchi
Masayo Date
Yukiko Umezawa
Keiichi Yokoyama
Haruo Heima
Hiroshi Matsui
Original Assignee
Ajinomoto Kk
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Ajinomoto Kk filed Critical Ajinomoto Kk
Application granted granted Critical
Publication of ATE447615T1 publication Critical patent/ATE447615T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
    • C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
    • C07K5/10—Tetrapeptides
    • C07K5/1002—Tetrapeptides with the first amino acid being neutral
    • C07K5/1016—Tetrapeptides with the first amino acid being neutral and aromatic or cycloaliphatic
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
AT02708684T 2001-03-30 2002-03-27 Verfahren zur sekretion und produktion von protein ATE447615T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2001098808 2001-03-30
PCT/JP2002/002978 WO2002081694A1 (fr) 2001-03-30 2002-03-27 Procede de secretion et de production de proteine

Publications (1)

Publication Number Publication Date
ATE447615T1 true ATE447615T1 (de) 2009-11-15

Family

ID=18952423

Family Applications (1)

Application Number Title Priority Date Filing Date
AT02708684T ATE447615T1 (de) 2001-03-30 2002-03-27 Verfahren zur sekretion und produktion von protein

Country Status (14)

Country Link
US (1) US7252972B2 (de)
EP (1) EP1375664B1 (de)
JP (1) JP4362651B2 (de)
KR (1) KR100602807B1 (de)
CN (1) CN1250722C (de)
AT (1) ATE447615T1 (de)
AU (1) AU2002242986A1 (de)
BR (1) BR0208136B1 (de)
CA (1) CA2442679C (de)
DE (1) DE60234247D1 (de)
DK (1) DK1375664T3 (de)
ES (1) ES2335750T3 (de)
RU (1) RU2264463C2 (de)
WO (1) WO2002081694A1 (de)

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HU229834B1 (en) 2000-01-21 2014-09-29 Ajinomoto Kk Process for producing l-lysine
RU2316594C2 (ru) * 2003-03-07 2008-02-10 Адзиномото Ко., Инк. Способ продуцирования микробной трансглутаминазы
DE10359660A1 (de) * 2003-12-18 2005-07-28 Basf Ag Psod-Expressionseinheiten
DE10359594A1 (de) * 2003-12-18 2005-07-28 Basf Ag PEF-TU-Expressionseinheiten
EP1748077B1 (de) * 2004-04-20 2013-02-27 Ajinomoto Co., Inc. Verfahren zur proteinherstellung
JP4809347B2 (ja) * 2004-06-14 2011-11-09 ノボザイムス アクティーゼルスカブ ポリペプチド製造用シグナルペプチド
US7527947B2 (en) 2004-06-14 2009-05-05 Novozymes A/S Signal peptide for producing a polypeptide
DE102004035065A1 (de) 2004-07-20 2006-02-16 Basf Ag P-ET-TS-Expressionseinheiten
KR100620092B1 (ko) * 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
US20070048793A1 (en) * 2005-07-12 2007-03-01 Baynes Brian M Compositions and methods for biocatalytic engineering
RU2338784C2 (ru) * 2006-03-24 2008-11-20 Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) Новая альдолаза, днк, кодирующая альдолазу, клетки, трансформированные днк, способ получения альдолазы и способ получения 4-гидрокси-l-изолейцина (варианты)
PL2082045T3 (pl) * 2006-10-24 2015-07-31 Basf Se Sposób zmniejszania ekspresji genów poprzez modyfikację częstości występowania kodonów
WO2008099898A1 (ja) 2007-02-15 2008-08-21 Ajinomoto Co., Inc. ジスルフィド結合導入トランスグルタミナーゼ
JP5093228B2 (ja) 2007-03-29 2012-12-12 味の素株式会社 接着用酵素製剤及び接着成形食品の製造方法
EP2213785A4 (de) 2007-11-19 2013-01-09 Ajinomoto Kk Verarbeitetes faserprodukt und herstellungsverfahren dafür
WO2010005099A1 (ja) * 2008-07-09 2010-01-14 味の素株式会社 アミノヒドロキシ安息香酸類の製造方法
TWI444146B (zh) 2008-09-25 2014-07-11 Ajinomoto Kk 黏著成型食品用酵素製劑及黏著成型食品之製造方法
WO2010101256A1 (ja) 2009-03-06 2010-09-10 味の素株式会社 放線菌由来の耐熱性トランスグルタミナーゼ
EP2540836B1 (de) 2010-02-23 2016-05-11 Toray Industries, Inc. Verfahren zur Herstellung von Cadaverin
CN103917656B (zh) * 2011-10-25 2016-09-28 味之素株式会社 蛋白质的分泌产生方法
AU2012333451B2 (en) 2011-11-02 2015-05-21 Ajinomoto Co., Inc. Method for secretory production of protein
IN2014CN04002A (de) 2011-11-02 2015-10-23 Ajinomoto Kk
JP2015519879A (ja) 2012-03-26 2015-07-16 プロニュートリア・インコーポレイテッドＰｒｏｎｕｔｒｉａ， Ｉｎｃ． 荷電栄養タンパク質および方法
JP2015518470A (ja) 2012-03-26 2015-07-02 プロニュートリア・インコーポレイテッドＰｒｏｎｕｔｒｉａ， Ｉｎｃ． 栄養タンパク質および方法
WO2013148325A1 (en) 2012-03-26 2013-10-03 Pronutria, Inc. Nutritive fragments, proteins and methods
CN104364259A (zh) 2012-03-26 2015-02-18 普罗努塔利亚公司 营养性片段、蛋白质和方法
RU2016110842A (ru) 2013-09-25 2017-10-30 Пронутриа Биосайенсис, Инк. Композиции и составы для предотвращения и уменьшения опухолеобразования, пролиферации и инвазии раковых клеток, и способы их получения и применения при лечении раковых заболеваний
DK3287523T3 (da) 2015-04-24 2022-07-25 Ajinomoto Kk Fremgangsmåde til sekretorisk fremstilling af protein
WO2017082374A1 (ja) 2015-11-12 2017-05-18 味の素株式会社 Ｎε－アシル－Ｌ－リジンの製造方法
WO2017191817A1 (ja) 2016-05-02 2017-11-09 味の素株式会社 アジド基含有Ｆｃタンパク質
ES2939979T3 (es) * 2016-09-01 2023-04-28 Ningxia Eppen Biotech Co Ltd Corynebacterium para producir L-lisina por fermentación
CN110312807B (zh) * 2016-10-21 2023-11-17 味之素株式会社 蛋白质的分泌产生方法
EP3530747A4 (de) 2016-10-21 2020-07-22 Ajinomoto Co., Inc. Verfahren zur herstellung von proteinsekreten
EP3572522A4 (de) 2017-01-19 2020-10-28 Ajinomoto Co., Inc. Verfahren zur herstellung einer heparosanverbindung mit isomerisiertem hexulonsäurerest
EP3415622A1 (de) 2017-06-14 2018-12-19 Evonik Degussa GmbH Verfahren zur herstellung von feinchemikalien mittels eines corynebakteriums, welches modifizierte alpha-1,6-glucosidasen sezerniert
CN117417912A (zh) 2017-09-05 2024-01-19 味之素株式会社 2-o-硫酸化酶突变体和3-o-硫酸化酶突变体及其使用方法
CN112004929B (zh) 2018-04-20 2024-03-15 味之素株式会社 蛋白质的分泌产生方法
EP3872096A4 (de) * 2018-10-25 2022-11-30 Ajinomoto Co., Inc. Verfahren zur sekretorischen herstellung eines proteins
EP3882261A4 (de) 2018-10-31 2023-02-08 Ajinomoto Co., Inc. Verbindung mit substanz mit affinität für antikörper, spaltstelle und reaktive gruppe oder salz davon
EP4115740A4 (de) 2020-03-04 2024-04-03 Ajinomoto Kk Mutante transglutaminase
WO2022071061A1 (ja) 2020-09-29 2022-04-07 味の素株式会社 変異型トランスグルタミナーゼ
WO2023282315A1 (ja) 2021-07-07 2023-01-12 味の素株式会社 非天然アミノ酸含有タンパク質の分泌生産法

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EP0158940B1 (de) * 1984-04-04 1991-12-11 Ajinomoto Co., Inc. Zusammengesetztes transduzierbares Plasmid
JPH07112431B2 (ja) * 1985-09-06 1995-12-06 味の素株式会社 遺伝子発現調節法
WO1988009821A1 (en) * 1987-06-12 1988-12-15 Massachusetts Institute Of Technology Coryneform expression and secretion system
US4965197A (en) * 1987-06-12 1990-10-23 Massachusetts Institute Of Technology Coryneform expression and secretion system
JPH06502548A (ja) * 1991-07-30 1994-03-24 オルサン 特にコリネバクテリア中で用いることのできる蛋白質の発現および分泌系
WO1994015963A1 (en) * 1993-01-13 1994-07-21 Ajinomoto Co., Inc. Novel cell cortex protein
JP3711658B2 (ja) 1996-10-07 2005-11-02 味の素株式会社 コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質
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ES2372806T3 (es) * 1999-09-30 2012-01-26 Ajinomoto Co., Inc. Proceso para la producción de transglutaminasa.

Also Published As

Publication number Publication date
KR100602807B1 (ko) 2006-07-20
ES2335750T3 (es) 2010-04-05
CN1500145A (zh) 2004-05-26
EP1375664B1 (de) 2009-11-04
EP1375664A1 (de) 2004-01-02
CN1250722C (zh) 2006-04-12
JP4362651B2 (ja) 2009-11-11
CA2442679A1 (en) 2002-10-17
WO2002081694A1 (fr) 2002-10-17
JPWO2002081694A1 (ja) 2004-07-29
WO2002081694A8 (fr) 2002-11-14
RU2264463C2 (ru) 2005-11-20
BR0208136B1 (pt) 2014-10-21
BR0208136A (pt) 2004-03-02
US20040126847A1 (en) 2004-07-01
EP1375664A4 (de) 2005-08-17
RU2003128977A (ru) 2005-02-27
AU2002242986A1 (en) 2002-10-21
DE60234247D1 (de) 2009-12-17
US7252972B2 (en) 2007-08-07
CA2442679C (en) 2010-06-01
DK1375664T3 (da) 2010-02-08
KR20030087042A (ko) 2003-11-12

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