BR0208136A - Método para produzir uma proteìna heteróloga - Google Patents
Método para produzir uma proteìna heterólogaInfo
- Publication number
- BR0208136A BR0208136A BR0208136-9A BR0208136A BR0208136A BR 0208136 A BR0208136 A BR 0208136A BR 0208136 A BR0208136 A BR 0208136A BR 0208136 A BR0208136 A BR 0208136A
- Authority
- BR
- Brazil
- Prior art keywords
- heterologous protein
- coryneform bacterium
- producing
- sequence encoding
- protein
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/36—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Actinomyces; from Streptomyces (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
- C07K14/34—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Corynebacterium (G)
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K5/00—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof
- C07K5/04—Peptides containing up to four amino acids in a fully defined sequence; Derivatives thereof containing only normal peptide links
- C07K5/10—Tetrapeptides
- C07K5/1002—Tetrapeptides with the first amino acid being neutral
- C07K5/1016—Tetrapeptides with the first amino acid being neutral and aromatic or cycloaliphatic
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
- C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K2319/00—Fusion polypeptide
Landscapes
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Molecular Biology (AREA)
- Biophysics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Gastroenterology & Hepatology (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biomedical Technology (AREA)
- Microbiology (AREA)
- Physics & Mathematics (AREA)
- Plant Pathology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Peptides Or Proteins (AREA)
- Enzymes And Modification Thereof (AREA)
Abstract
"MéTODO PARA PRODUZIR UMA PROTEìNA HETERóLOGA". O objeto da presente invenção é prover um método de produção de uma proteína heteróloga ao fazer uma bactéria corineforme produzir e secretar, de modo eficiente extracelularmente (secreto-produção), uma proteína heteróloga industrialmente utilizável. De acordo com a presente invenção, uma construção genética é usada onde uma seq³ência de genes codificando uma proteina pretendida é ligada a montante de uma seq³ência codificando o peptídeo sinal derivado de uma bactéria corineforme, a construção de gene é introduzida em uma bactéria corineforme mutante que tem a capacidade de secretar a proteína heteróloga pelo menos 2 vezes maior do que Corynebacterium glutamicum ATCC 13869 de tipo selvagem, a bactéria corineforme mutante sendo cultivada e a proteína heteróloga liberada extracelularmente sendo recuperada.
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2001-98808 | 2001-03-30 | ||
| JP2001098808 | 2001-03-30 | ||
| PCT/JP2002/002978 WO2002081694A1 (fr) | 2001-03-30 | 2002-03-27 | Procede de secretion et de production de proteine |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BR0208136A true BR0208136A (pt) | 2004-03-02 |
| BR0208136B1 BR0208136B1 (pt) | 2014-10-21 |
Family
ID=18952423
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0208136-9A BR0208136B1 (pt) | 2001-03-30 | 2002-03-27 | Método para produzir uma proteína heteróloga |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US7252972B2 (pt) |
| EP (1) | EP1375664B1 (pt) |
| JP (1) | JP4362651B2 (pt) |
| KR (1) | KR100602807B1 (pt) |
| CN (1) | CN1250722C (pt) |
| AT (1) | ATE447615T1 (pt) |
| AU (1) | AU2002242986A1 (pt) |
| BR (1) | BR0208136B1 (pt) |
| CA (1) | CA2442679C (pt) |
| DE (1) | DE60234247D1 (pt) |
| DK (1) | DK1375664T3 (pt) |
| ES (1) | ES2335750T3 (pt) |
| RU (1) | RU2264463C2 (pt) |
| WO (1) | WO2002081694A1 (pt) |
Families Citing this family (42)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| SK287800B6 (sk) | 2000-01-21 | 2011-10-04 | Ajinomoto Co., Inc. | Spôsob výroby L-lyzínu |
| BRPI0407911A (pt) * | 2003-03-07 | 2006-02-14 | Ajinomoto Kk | método para produzir uma transglutaminase ativa microbiana a partir de uma pró-transglutaminase microbiana, metaloprotease neutra de actionomicetos, molécula de ácido nucleico, e, método para produzir uma metaloprotease neutra de actinomicetos |
| DE10359594A1 (de) * | 2003-12-18 | 2005-07-28 | Basf Ag | PEF-TU-Expressionseinheiten |
| DE10359660A1 (de) * | 2003-12-18 | 2005-07-28 | Basf Ag | Psod-Expressionseinheiten |
| WO2005103278A1 (ja) | 2004-04-20 | 2005-11-03 | Ajinomoto Co., Inc. | タンパク質の製造法 |
| US7527947B2 (en) | 2004-06-14 | 2009-05-05 | Novozymes A/S | Signal peptide for producing a polypeptide |
| WO2005121333A1 (en) * | 2004-06-14 | 2005-12-22 | Novozymes A/S | Signal peptide for producing a polypeptide |
| DE102004035065A1 (de) | 2004-07-20 | 2006-02-16 | Basf Ag | P-ET-TS-Expressionseinheiten |
| KR100620092B1 (ko) * | 2004-12-16 | 2006-09-08 | 씨제이 주식회사 | 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법 |
| EP1902126A1 (en) * | 2005-07-12 | 2008-03-26 | Codon Devices, Inc | Compositions and methods for biocatalytic engineering |
| RU2338784C2 (ru) * | 2006-03-24 | 2008-11-20 | Закрытое акционерное общество "Научно-исследовательский институт Аджиномото-Генетика" (ЗАО АГРИ) | Новая альдолаза, днк, кодирующая альдолазу, клетки, трансформированные днк, способ получения альдолазы и способ получения 4-гидрокси-l-изолейцина (варианты) |
| HUE025440T2 (en) * | 2006-10-24 | 2016-04-28 | Basf Se | A method for reducing gene expression using modified codon usage |
| EP2123756B1 (en) * | 2007-02-15 | 2014-06-04 | Ajinomoto Co., Inc. | Transglutaminase having disulfide bond introduced therein |
| PL2138049T3 (pl) | 2007-03-29 | 2014-11-28 | Ajinomoto Kk | Preparat enzymatyczny do adhezji i sposób wytwarzania uformowanego adhezyjnie produktu żywnościowego |
| WO2009066785A1 (ja) | 2007-11-19 | 2009-05-28 | Ajinomoto Co., Inc. | 繊維加工物及びその製造法 |
| EP2302066A4 (en) * | 2008-07-09 | 2012-02-08 | Ajinomoto Kk | METHOD FOR PRODUCING AMINOHYDROXYBENZOIC ACID |
| TWI444146B (zh) | 2008-09-25 | 2014-07-11 | Ajinomoto Kk | 黏著成型食品用酵素製劑及黏著成型食品之製造方法 |
| EP2404999B1 (en) | 2009-03-06 | 2015-04-29 | Ajinomoto Co., Inc. | Thermotolerant transglutaminase originating in actinomyces |
| BR112012021070B1 (pt) | 2010-02-23 | 2020-02-27 | Toray Industries Inc. | Método para produzir cadaverina |
| JP6064912B2 (ja) | 2011-10-25 | 2017-01-25 | 味の素株式会社 | タンパク質の分泌生産法 |
| IN2014CN04002A (pt) | 2011-11-02 | 2015-10-23 | Ajinomoto Kk | |
| WO2013065869A1 (en) | 2011-11-02 | 2013-05-10 | Ajinomoto Co.,Inc. | Method for secretory production of protein |
| CN104364259A (zh) | 2012-03-26 | 2015-02-18 | 普罗努塔利亚公司 | 营养性片段、蛋白质和方法 |
| WO2013148328A1 (en) | 2012-03-26 | 2013-10-03 | Pronutria, Inc. | Nutritive proteins and methods |
| JP2015519878A (ja) | 2012-03-26 | 2015-07-16 | プロニュートリア・インコーポレイテッドＰｒｏｎｕｔｒｉａ， Ｉｎｃ． | 栄養断片、タンパク質、および方法 |
| SG10201604464SA (en) | 2012-03-26 | 2016-07-28 | Axcella Health Inc | Charged nutritive proteins and methods |
| EP3048904A2 (en) | 2013-09-25 | 2016-08-03 | Pronutria Biosciences, Inc. | Compositions and formulations for treatment of gastrointestinal tract malabsorption diseases and inflammatory conditions and methods of production and use thereof |
| JP6741000B2 (ja) | 2015-04-24 | 2020-08-19 | 味の素株式会社 | タンパク質の分泌生産法 |
| JP6729600B2 (ja) | 2015-11-12 | 2020-07-22 | 味の素株式会社 | Ｎε−アシル−Ｌ−リジンの製造方法 |
| WO2017191817A1 (ja) | 2016-05-02 | 2017-11-09 | 味の素株式会社 | アジド基含有Ｆｃタンパク質 |
| JP6739651B2 (ja) * | 2016-09-01 | 2020-08-12 | ニンシア エッペン バイオテック カンパニー リミテッド | Ｌ−リジンを発酵生産するコリネバクテリウム菌 |
| EP3530748B1 (en) | 2016-10-21 | 2021-06-02 | Ajinomoto Co., Inc. | Method for the secretory production proteins |
| EP3530747A4 (en) | 2016-10-21 | 2020-07-22 | Ajinomoto Co., Inc. | SECRETORY PROTEIN PRODUCTION PROCESS |
| EP3572522A4 (en) | 2017-01-19 | 2020-10-28 | Ajinomoto Co., Inc. | PROCESS FOR THE PRODUCTION OF A HEPAROSANE COMPOUND CONTAINING A RESIDUE OF ISOMERIZED HEXULONIC ACID |
| EP3415622A1 (en) | 2017-06-14 | 2018-12-19 | Evonik Degussa GmbH | Method for production of fine chemicals using a corynebacterium secreting modified alpha-1,6-glucosidases |
| EP4050036A1 (en) | 2017-09-05 | 2022-08-31 | Ajinomoto Co., Inc. | 3-o-sulfation enzyme mutant, and method for using same |
| AU2019256065A1 (en) | 2018-04-20 | 2020-11-26 | Ajinomoto Co., Inc. | Method for secretory production of protein |
| EP3872096A4 (en) | 2018-10-25 | 2022-11-30 | Ajinomoto Co., Inc. | PROCESS FOR THE SECRETORY PRODUCTION OF A PROTEIN |
| EP3882261A4 (en) | 2018-10-31 | 2023-02-08 | Ajinomoto Co., Inc. | COMPOUND COMPRISING A SUBSTANCE HAVING AN AFFINITY FOR AN ANTIBODY, CLEAVAGE SITE AND CORRESPONDING REACTIVE GROUP OR SALT |
| BR112022017430A2 (pt) | 2020-03-04 | 2022-10-18 | Ajinomoto Kk | Método e composição para produzir um alimento, transglutaminase mutante, gene, vetor, e, microrganismo |
| BR112023005635A2 (pt) | 2020-09-29 | 2023-04-25 | Ajinomoto Kk | Método e composição para produzir um alimento, transglutaminase mutante, gene, vetor, e, microrganismo |
| KR20240032941A (ko) | 2021-07-07 | 2024-03-12 | 아지노모토 가부시키가이샤 | 비천연 아미노산 함유 단백질의 분비 생산법 |
Family Cites Families (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| EP0158940B1 (en) | 1984-04-04 | 1991-12-11 | Ajinomoto Co., Inc. | Transducible composite plasmid |
| JPH07112431B2 (ja) * | 1985-09-06 | 1995-12-06 | 味の素株式会社 | 遺伝子発現調節法 |
| US4965197A (en) | 1987-06-12 | 1990-10-23 | Massachusetts Institute Of Technology | Coryneform expression and secretion system |
| WO1988009821A1 (en) * | 1987-06-12 | 1988-12-15 | Massachusetts Institute Of Technology | Coryneform expression and secretion system |
| WO1993003158A1 (fr) * | 1991-07-30 | 1993-02-18 | Orsan | Systeme d'expression et de secretion de proteines utilisables en particulier chez les corynebacteries |
| DE69409895T2 (de) | 1993-01-13 | 1998-12-24 | Ajinomoto Kk | Zelloberflächenprotein aus brevibakterium lactofermentum |
| JP3711658B2 (ja) | 1996-10-07 | 2005-11-02 | 味の素株式会社 | コリネバクテリウム・アンモニアゲネス由来の新規な細胞表層蛋白質 |
| EP0897003B1 (en) | 1997-07-05 | 2004-05-12 | Societe Des Produits Nestle S.A. | Enhanced expression of proteolytic enzymes in koji mold |
| JPH11169182A (ja) * | 1997-12-10 | 1999-06-29 | Mitsubishi Chemical Corp | コリネ型細菌内で蛋白質を効率よく培地中へ分泌させる変異型分泌装置遺伝子 |
| KR100565030B1 (ko) * | 1999-09-30 | 2006-03-30 | 아지노모토 가부시키가이샤 | 트랜스글루타미나제의 제조방법 |
-
2002
- 2002-03-27 WO PCT/JP2002/002978 patent/WO2002081694A1/ja active Application Filing
- 2002-03-27 AU AU2002242986A patent/AU2002242986A1/en not_active Abandoned
- 2002-03-27 CA CA2442679A patent/CA2442679C/en not_active Expired - Lifetime
- 2002-03-27 EP EP02708684A patent/EP1375664B1/en not_active Expired - Lifetime
- 2002-03-27 ES ES02708684T patent/ES2335750T3/es not_active Expired - Lifetime
- 2002-03-27 JP JP2002580057A patent/JP4362651B2/ja not_active Expired - Lifetime
- 2002-03-27 DK DK02708684.2T patent/DK1375664T3/da active
- 2002-03-27 RU RU2003128977/13A patent/RU2264463C2/ru active
- 2002-03-27 DE DE60234247T patent/DE60234247D1/de not_active Expired - Lifetime
- 2002-03-27 KR KR1020037012833A patent/KR100602807B1/ko not_active IP Right Cessation
- 2002-03-27 AT AT02708684T patent/ATE447615T1/de not_active IP Right Cessation
- 2002-03-27 BR BRPI0208136-9A patent/BR0208136B1/pt active IP Right Grant
- 2002-03-27 CN CNB028076265A patent/CN1250722C/zh not_active Expired - Lifetime
-
2003
- 2003-09-30 US US10/673,860 patent/US7252972B2/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| RU2003128977A (ru) | 2005-02-27 |
| KR20030087042A (ko) | 2003-11-12 |
| US7252972B2 (en) | 2007-08-07 |
| DK1375664T3 (da) | 2010-02-08 |
| WO2002081694A1 (fr) | 2002-10-17 |
| CA2442679C (en) | 2010-06-01 |
| RU2264463C2 (ru) | 2005-11-20 |
| JP4362651B2 (ja) | 2009-11-11 |
| DE60234247D1 (de) | 2009-12-17 |
| CA2442679A1 (en) | 2002-10-17 |
| CN1500145A (zh) | 2004-05-26 |
| KR100602807B1 (ko) | 2006-07-20 |
| JPWO2002081694A1 (ja) | 2004-07-29 |
| EP1375664A4 (en) | 2005-08-17 |
| WO2002081694A8 (fr) | 2002-11-14 |
| BR0208136B1 (pt) | 2014-10-21 |
| ATE447615T1 (de) | 2009-11-15 |
| EP1375664B1 (en) | 2009-11-04 |
| EP1375664A1 (en) | 2004-01-02 |
| ES2335750T3 (es) | 2010-04-05 |
| CN1250722C (zh) | 2006-04-12 |
| US20040126847A1 (en) | 2004-07-01 |
| AU2002242986A1 (en) | 2002-10-21 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B06A | Patent application procedure suspended [chapter 6.1 patent gazette] | ||
| B09A | Decision: intention to grant [chapter 9.1 patent gazette] | ||
| B16A | Patent or certificate of addition of invention granted [chapter 16.1 patent gazette] |
Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 21/10/2014, OBSERVADAS AS CONDICOES LEGAIS. |