WO2002055711A3 - Verfahren zur fermentativen herstellung von pantothensäure - Google Patents

Verfahren zur fermentativen herstellung von pantothensäure Download PDF

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Publication number
WO2002055711A3
WO2002055711A3 PCT/DE2001/004955 DE0104955W WO02055711A3 WO 2002055711 A3 WO2002055711 A3 WO 2002055711A3 DE 0104955 W DE0104955 W DE 0104955W WO 02055711 A3 WO02055711 A3 WO 02055711A3
Authority
WO
WIPO (PCT)
Prior art keywords
pantothenic acid
production
reduction
transaminase
fermentation
Prior art date
Application number
PCT/DE2001/004955
Other languages
English (en)
French (fr)
Other versions
WO2002055711A2 (de
Inventor
Lothar Eggeling
Hermann Sahm
Original Assignee
Forschungszentrum Juelich Gmbh
Lothar Eggeling
Hermann Sahm
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Forschungszentrum Juelich Gmbh, Lothar Eggeling, Hermann Sahm filed Critical Forschungszentrum Juelich Gmbh
Publication of WO2002055711A2 publication Critical patent/WO2002055711A2/de
Publication of WO2002055711A3 publication Critical patent/WO2002055711A3/de

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1096—Transferases (2.) transferring nitrogenous groups (2.6)

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  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

Die Erfindung betrifft in Mikroorganismen der Gattung Corynebacterium replizierbare, gegebenenfalls rekombinante DNA. Sie betrifft weiterin ein Verfahren zur fermentativen Herstellung von Pantothensäure. Erfindungsgemäss wird durch eine Abschwächung oder Ausschaltung der Transaminase eine erhöhte Pantothensäurebildung möglich. Dies kann durch Deletion oder eine geringere Expression der für Transaminase codierenden Gensequenzen erreicht werden. Beispielsweise konnte durch Deleton oder Reduzierung der Expression des neuisolierten D-Pantothenatbiosynthesegens brnA aus Corynebacterium glutamicum in verbesserter Weise Panthotenat produziert werden. Mit Hilfe der erfindungsgemässen Nukleotidsequenz und dem Verfahren wird es möglish, durch Reduzierung der Transaminaseaktivität, eine verstärkte Pantothensäureproduktion zu erreichen.
PCT/DE2001/004955 2001-01-12 2001-12-22 Verfahren zur fermentativen herstellung von pantothensäure WO2002055711A2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DE10101501.1 2001-01-12
DE10101501A DE10101501A1 (de) 2001-01-12 2001-01-12 Verfahren zur fermentativen Herstellung von Pantothensäure

Publications (2)

Publication Number Publication Date
WO2002055711A2 WO2002055711A2 (de) 2002-07-18
WO2002055711A3 true WO2002055711A3 (de) 2002-12-19

Family

ID=7670564

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/DE2001/004955 WO2002055711A2 (de) 2001-01-12 2001-12-22 Verfahren zur fermentativen herstellung von pantothensäure

Country Status (2)

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DE (1) DE10101501A1 (de)
WO (1) WO2002055711A2 (de)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2007131750A1 (en) 2006-05-16 2007-11-22 Dsm Ip Assets B.V. Process for the production of panthenol
CN113913478B (zh) * 2021-11-26 2023-06-02 江南大学 一种发酵黄色短杆菌产l-缬氨酸的方法

Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001000843A2 (en) * 1999-06-25 2001-01-04 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding metabolic pathway proteins
EP1217069A2 (de) * 2000-12-20 2002-06-26 Degussa AG Für das ilvE-Gen kodierende Nukleotidsequenzen

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE19846499A1 (de) * 1998-10-09 2000-04-20 Degussa Verfahren zur Herstellung von Pantothensäure durch Verstärkung von für Ketopantoat-Reduktase kodierenden Nukleotidsequenzen
BRPI0014115B8 (pt) * 1999-09-21 2021-05-25 Basf Ag métodos de produzir um panto-composto, pantotenato e beta-alanina, de aumentar a produção de um panto-composto e de edentificar compostos que modulam a atividade da pantotenato cinase, micro-organismo recombinante, vetor recombinante, e, vetor
DE10014546A1 (de) * 2000-03-23 2001-09-27 Degussa Für das dapC-Gen kodierende Nukleotidsequenzen und Verfahren zur Herstellung von L-Lysin

Patent Citations (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2001000843A2 (en) * 1999-06-25 2001-01-04 Basf Aktiengesellschaft Corynebacterium glutamicum genes encoding metabolic pathway proteins
EP1217069A2 (de) * 2000-12-20 2002-06-26 Degussa AG Für das ilvE-Gen kodierende Nukleotidsequenzen

Non-Patent Citations (4)

* Cited by examiner, † Cited by third party
Title
BERNHARD J EIKMANNS ET AL: "A family of Corynebacterium glutamicum/Escherichia coli shuttle vectors for cloning, controlled gene expression, and promoter probing", GENE: AN INTERNATIONAL JOURNAL ON GENES AND GENOMES, ELSEVIER SCIENCE PUBLISHERS, BARKING, GB, vol. 102, 1991, pages 93 - 98, XP002153371, ISSN: 0378-1119 *
DATABASE EMBL [online] 24 January 2001 (2001-01-24), XP002215341, retrieved from EBI Database accession no. AX063979 *
SAHM H ET AL: "D-Pantothenate synthesis in Corynebacterium glutamicum and use of panBC and genes encoding L-valine synthesis for D-pantothenate overproduction", APPLIED AND ENVIRONMENTAL MICROBIOLOGY, WASHINGTON,DC, US, vol. 65, no. 5, May 1999 (1999-05-01), pages 1973 - 1979, XP002169517, ISSN: 0099-2240 *
SCHAEFER A ET AL: "SMALL MOBILIZABLE MULTI-PURPOSE CLONING VECTORS DERIVED FROM THE ESCHERICHIA COLI PLASMIDS PK18 AND PK19: SELECTION OF DEFINED DELETIONS IN THE CHROMOSOME OF CORYNEBACTERIUM GLUTAMICUM", GENE, ELSEVIER BIOMEDICAL PRESS. AMSTERDAM, NL, vol. 145, no. 145, 1994, pages 69 - 73, XP001093898, ISSN: 0378-1119 *

Also Published As

Publication number Publication date
WO2002055711A2 (de) 2002-07-18
DE10101501A1 (de) 2002-08-01

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