BRPI0014115B8 - métodos de produzir um panto-composto, pantotenato e beta-alanina, de aumentar a produção de um panto-composto e de edentificar compostos que modulam a atividade da pantotenato cinase, micro-organismo recombinante, vetor recombinante, e, vetor - Google Patents

métodos de produzir um panto-composto, pantotenato e beta-alanina, de aumentar a produção de um panto-composto e de edentificar compostos que modulam a atividade da pantotenato cinase, micro-organismo recombinante, vetor recombinante, e, vetor

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Publication number
BRPI0014115B8
BRPI0014115B8 BRPI0014115A BR0014115A BRPI0014115B8 BR PI0014115 B8 BRPI0014115 B8 BR PI0014115B8 BR PI0014115 A BRPI0014115 A BR PI0014115A BR 0014115 A BR0014115 A BR 0014115A BR PI0014115 B8 BRPI0014115 B8 BR PI0014115B8
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Brazil
Prior art keywords
pantothenate
methods
microorganisms
producing
recombinant
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BRPI0014115A
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English (en)
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BR0014115A (pt
BRPI0014115B1 (pt
Inventor
G Pero Janice
Rogers Yocum R
Hermann Theron
A Patterson Thomas
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Basf Ag
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Application filed by Basf Ag filed Critical Basf Ag
Publication of BR0014115A publication Critical patent/BR0014115A/pt
Publication of BRPI0014115B1 publication Critical patent/BRPI0014115B1/pt
Publication of BRPI0014115B8 publication Critical patent/BRPI0014115B8/pt

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    • C—CHEMISTRY; METALLURGY
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    • C12P21/00—Preparation of peptides or proteins
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/02—Amides, e.g. chloramphenicol or polyamides; Imides or polyimides; Urethanes, i.e. compounds comprising N-C=O structural element or polyurethanes
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    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0006—Oxidoreductases (1.) acting on CH-OH groups as donors (1.1)
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1014—Hydroxymethyl-, formyl-transferases (2.1.2)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
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    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
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    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
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    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/06—Alanine; Leucine; Isoleucine; Serine; Homoserine
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    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/04—Oxygen as only ring hetero atoms containing a five-membered hetero ring, e.g. griseofulvin, vitamin C
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    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/42—Hydroxy-carboxylic acids

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  • Chemical & Material Sciences (AREA)
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  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"métodos de produzir um panto-composto, pantotenato e beta-alanina, de aumentar a produção de um panto-composto e de identificar compostos que modulam a atividade da pantotenato cinase, microorganismo recombinante, vetor, molécula de ácido nucleico isolada, polipetídeo e proteína da pantotenato cinase isolada". a presente invenção retrata métodos de produzir panto-compostos (por exemplo, pantotenato) usando microorganismos em que o caminho biossintétíco do pantotenato e/ou o caminho biossintético da isoleucina-valina e/ou o caminho biossintético da coenzimaa tenha sido manipulado. métodos que retratam microorganismos que supraexpressam a cetopantoato reductase bem como microorganismos que supraexpressam a aspartato <244>-descarboxilase são fornecidos. métodos de produzir panto-compostos em uma maneira independente de precursor e em alto rendimento são descritos. microorganismos recombinantes, vetores, moléculas de ácido nucleico isoladas, genes e produtos de gene úteis na prática das metodologias acima também são fornecidos. a presente invenção também retrata um gene da pantotenato cinase microbiana não identificado anteriormente, coax, bem como métodos de produzir panto-compostos que utilizam microorganismos tendo atividade modificada da pantotenato cinase. microorganismos recombinantes, vetores, moléculas de ácido nucleico coax isoladas e proteínas coax purificadas são retratados. também são retratados métodos para identificar os moduladores da pantotenato cinase que utilizam os microorganismos recombinantes e/ou as proteínas coax purificadas da presente invenção.
BRPI0014115A 1999-09-21 2000-09-21 métodos de produzir um panto-composto, pantotenato e beta-alanina, de aumentar a produção de um panto-composto e de edentificar compostos que modulam a atividade da pantotenato cinase, micro-organismo recombinante, vetor recombinante, e, vetor BRPI0014115B8 (pt)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
US40049499A 1999-09-21 1999-09-21
US21007200P 2000-06-07 2000-06-07
US22183600P 2000-07-28 2000-07-28
US22786000P 2000-08-24 2000-08-24
PCT/US2000/025993 WO2001021772A2 (en) 1999-09-21 2000-09-21 Methods and microorganisms for production of panto-compounds

Publications (3)

Publication Number Publication Date
BR0014115A BR0014115A (pt) 2002-05-21
BRPI0014115B1 BRPI0014115B1 (pt) 2015-05-05
BRPI0014115B8 true BRPI0014115B8 (pt) 2021-05-25

Family

ID=27498789

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BRPI0014115A BRPI0014115B8 (pt) 1999-09-21 2000-09-21 métodos de produzir um panto-composto, pantotenato e beta-alanina, de aumentar a produção de um panto-composto e de edentificar compostos que modulam a atividade da pantotenato cinase, micro-organismo recombinante, vetor recombinante, e, vetor

Country Status (16)

Country Link
EP (2) EP2163629B1 (pt)
JP (1) JP5392957B2 (pt)
KR (1) KR20020092912A (pt)
CN (1) CN1420931A (pt)
AU (1) AU7708700A (pt)
BR (1) BRPI0014115B8 (pt)
CA (1) CA2385497A1 (pt)
CZ (1) CZ20021013A3 (pt)
HU (1) HU230509B1 (pt)
IL (1) IL148786A0 (pt)
MX (1) MXPA02003017A (pt)
NO (1) NO20021382L (pt)
PL (1) PL356566A1 (pt)
SK (1) SK5222002A3 (pt)
TR (1) TR200200751T2 (pt)
WO (1) WO2001021772A2 (pt)

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Publication number Publication date
JP2003527828A (ja) 2003-09-24
WO2001021772A3 (en) 2002-03-07
CA2385497A1 (en) 2001-03-29
EP1214420B1 (en) 2016-03-23
EP2163629A3 (en) 2010-06-02
HUP0202705A2 (hu) 2002-12-28
HUP0202705A3 (en) 2011-02-28
BR0014115A (pt) 2002-05-21
IL148786A0 (en) 2002-09-12
JP5392957B2 (ja) 2014-01-22
PL356566A1 (en) 2004-06-28
EP1214420A2 (en) 2002-06-19
WO2001021772A2 (en) 2001-03-29
CZ20021013A3 (en) 2002-12-11
NO20021382L (no) 2002-05-16
TR200200751T2 (tr) 2004-08-23
SK5222002A3 (en) 2003-03-04
NO20021382D0 (no) 2002-03-20
MXPA02003017A (es) 2004-09-06
CN1420931A (zh) 2003-05-28
EP2163629B1 (en) 2017-03-08
WO2001021772A9 (en) 2002-12-05
BRPI0014115B1 (pt) 2015-05-05
AU7708700A (en) 2001-04-24
EP2163629A2 (en) 2010-03-17
HU230509B1 (hu) 2016-09-28
KR20020092912A (ko) 2002-12-12

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