BR0210870A - Aminopeptidase derivada de bacillus licheniformis, gene codificando a aminopeptidase vetor de expressão contendo o gene, transformante e método para preparação destes - Google Patents

Aminopeptidase derivada de bacillus licheniformis, gene codificando a aminopeptidase vetor de expressão contendo o gene, transformante e método para preparação destes

Info

Publication number
BR0210870A
BR0210870A BR0210870-4A BR0210870A BR0210870A BR 0210870 A BR0210870 A BR 0210870A BR 0210870 A BR0210870 A BR 0210870A BR 0210870 A BR0210870 A BR 0210870A
Authority
BR
Brazil
Prior art keywords
aminopeptidase
expression vector
gene
vector containing
preparing
Prior art date
Application number
BR0210870-4A
Other languages
English (en)
Inventor
Young-Phil Lee
Seung-Won Lee
Chul-Ho Jung
Hyung-Cheol Kim
Soon-Yong Choi
Jin-Suk Kim
Hyun-Sik Kim
Jung-Woo Seo
Original Assignee
Lg Life Sciences Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from KR10-2002-0030798A external-priority patent/KR100477062B1/ko
Application filed by Lg Life Sciences Ltd filed Critical Lg Life Sciences Ltd
Publication of BR0210870A publication Critical patent/BR0210870A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"AMINOPEPTIDASE DERIVADA DE BACILLUS LICHENIFORMIS, GENE CODIFICANDO A AMINOPEPTIDASE VETOR DE EXPRESSãO CONTENDO O GENE, TRANSFORMANTE E MéTODO PARA PREPARAçãO DESTES". A presente invenção refere-se a nova aminopeptidase derivada de Bacillus licheniformis, um gene codificando a aminopeptidase, um vetor de expressão contendo o gene, um transformante de célula transfectado com o vetor de expressão e um processo para preparar uma proteína do tipo natural usando estes. Mais particularmente, a presente invenção refere-se a um gene codificando a aminopeptidase que é clonada e produzida usando a técnica de DNA recombinante, um vetor de expressão contendo o gene, um transformante de célula transfectado com o vetor de expressão e uma aminopeptidase recombinante que é necessária para produzir um hormónio de crescimento humano recombinante em uma proteína do tipo natural e pode ser expressa em um alto rendimento mais estável e vantajosamente, comparado com métodos convencionais para a purificação.
BR0210870-4A 2001-07-06 2002-07-06 Aminopeptidase derivada de bacillus licheniformis, gene codificando a aminopeptidase vetor de expressão contendo o gene, transformante e método para preparação destes BR0210870A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20010040268 2001-07-06
KR10-2002-0030798A KR100477062B1 (ko) 2001-07-06 2002-05-31 바실러스 리케니포미스 유래의 신규한 아미노펩티다아제, 이를 코딩하는 유전자, 이 유전자를 포함하는 발현벡터, 이 발현벡터로 형질전환된 형질전환체 및 이를 이용하는 천연형 단백질의 제조방법
PCT/KR2002/001280 WO2003004635A1 (en) 2001-07-06 2002-07-06 Novel aminopeptidase derived from bacillus licheniformis, gene encoding the aminopeptidase, expression vector containing the gene, transformant and method for preparation thereof

Publications (1)

Publication Number Publication Date
BR0210870A true BR0210870A (pt) 2004-06-22

Family

ID=26639209

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0210870-4A BR0210870A (pt) 2001-07-06 2002-07-06 Aminopeptidase derivada de bacillus licheniformis, gene codificando a aminopeptidase vetor de expressão contendo o gene, transformante e método para preparação destes

Country Status (10)

Country Link
US (1) US7098018B2 (pt)
EP (1) EP1404829B1 (pt)
JP (1) JP4580644B2 (pt)
AT (1) ATE380863T1 (pt)
BR (1) BR0210870A (pt)
CA (1) CA2451528C (pt)
DK (1) DK1404829T3 (pt)
ES (1) ES2295370T3 (pt)
PL (1) PL205888B1 (pt)
WO (1) WO2003004635A1 (pt)

Families Citing this family (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5319543B2 (ja) * 2007-10-29 2013-10-16 日本ケミカルリサーチ株式会社 アミノペプチダーゼの製造方法
WO2010111622A2 (en) * 2009-03-27 2010-09-30 Codexis, Inc. Amidases and methods of their use

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4755465A (en) * 1983-04-25 1988-07-05 Genentech, Inc. Secretion of correctly processed human growth hormone in E. coli and Pseudomonas
DK589785A (da) * 1985-12-18 1987-06-19 Samuelsson Ernst Gunnar Peptidpraeparat, fremgangsmaade til fremstilling deraf samt anvendelse af peptidpraeparatet
US5108919A (en) 1988-06-24 1992-04-28 Genentech, Inc. Dna sequences encoding yeast ubiquitin hydrolase
US5126249A (en) * 1989-05-09 1992-06-30 Eli Lilly And Company Enzymatic removal of a protein amino-terminal sequence
KR100369839B1 (ko) * 1997-02-28 2003-06-12 주식회사 엘지생명과학 바실러스리케니포미스균주에서유래한아미노펩티다제,그의제조방법및이를이용한천연형단백질의제조방법
US7018794B2 (en) * 2000-10-06 2006-03-28 Novozymes, Inc. Methods for monitoring multiple gene expression

Also Published As

Publication number Publication date
JP4580644B2 (ja) 2010-11-17
CA2451528C (en) 2011-08-16
US7098018B2 (en) 2006-08-29
JP2004533263A (ja) 2004-11-04
DK1404829T3 (da) 2008-03-25
US20040253703A1 (en) 2004-12-16
EP1404829A4 (en) 2005-06-08
WO2003004635A1 (en) 2003-01-16
ATE380863T1 (de) 2007-12-15
EP1404829A1 (en) 2004-04-07
PL367768A1 (en) 2005-03-07
PL205888B1 (pl) 2010-06-30
EP1404829B1 (en) 2007-12-12
CA2451528A1 (en) 2003-01-16
ES2295370T3 (es) 2008-04-16

Similar Documents

Publication Publication Date Title
PT1885850E (pt) Método de produção de d-psicose através de d-psicose epimerase
ATE455855T1 (de) Gene von corynebacterium glutamicum kodierend für stoffwechselproteine
IL175021A0 (en) Chromatin insulator, its preparation and use in protein expression
DK0445646T3 (da) DNA-fragment, som koder for et polypeptid med nitrilhydrataseaktivitet, en transformant indeholdende DNA-fragmentet og en fremgangsmåde til fremstilling af amider under anvendelse af transformanten
AU2390301A (en) Corynebacterium glutamicum genes encoding metabolic pathway proteins
HK1054765A1 (en) Metabolic rate shifts in fermentations expressing recombinant proteins
DE602005020972D1 (en) Rhamnosepromotor-expressionssystem
WO2004097001A3 (en) Novel bacillus 029cel cellulase
ATE362544T1 (de) Polypeptide mit alpha-h alpha amino acid amide racemase aktivitität und nukleinsäuren kodierend dafür
NZ508863A (en) Generation of multiple gene products from chimeric fusion proteins by cleavage with ubiquitous endoproteases
DK1481052T3 (da) Hyaluronan-syntase-gener og ekspression deraf
ATE464320T1 (de) Ntb-a, ein oberflächenmolekül impliziert in der aktivität natürlicher killerzellen (nk)
ID22189A (id) Protein yang berperan dalam pengaktifan nitril hidratase dan gen yang menyandikannya
BR0210870A (pt) Aminopeptidase derivada de bacillus licheniformis, gene codificando a aminopeptidase vetor de expressão contendo o gene, transformante e método para preparação destes
DK0387646T3 (da) Achromobacter-protease I-gen og genprodukt deraf
ATE327321T1 (de) Zyklische depsipeptid-synthasen, deren gene und system zur massenproduktion von zyklischen depsipeptiden
TW200600581A (en) Nucleic acid construct and expression vector for enhancing the production of recombinant protein, and method for the massive production of recombinant protein
DK1545428T3 (da) Fremgangsmåde til formindskelse af aggregatmængder af pegylerede proteiner
EP1721976A4 (en) MODIFIED PROMOTER
HK1089457A1 (en) Recombinant igf expression systems
DE60040077D1 (de) Vermehrungsproteine von pflanzen
BR9807616A (pt) Aminopeptidase derivada de bacillus licheniformis e processo para preparação de proteìnas tipo natural
ATE318920T1 (de) Promotoren zur genexpression in karyopsen von pflanzen
MX2008014653A (es) Leucina aminopeptidasa recombinante, su gen codificante, vector de expresion que contine el gen, transformante, su metodo de purificacion y su uso para la remocion de la metionina n-terminal de interferon a-2b recombinante.

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 13A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2313 DE 05-05-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.