DK0576294T3 - Chondroitinase, fremgangsmåde til fremstilling af chondroitinase og farmaceutisk præparat indeholdende chondroitinase - Google Patents

Chondroitinase, fremgangsmåde til fremstilling af chondroitinase og farmaceutisk præparat indeholdende chondroitinase

Info

Publication number
DK0576294T3
DK0576294T3 DK93304979T DK93304979T DK0576294T3 DK 0576294 T3 DK0576294 T3 DK 0576294T3 DK 93304979 T DK93304979 T DK 93304979T DK 93304979 T DK93304979 T DK 93304979T DK 0576294 T3 DK0576294 T3 DK 0576294T3
Authority
DK
Denmark
Prior art keywords
chondroitinase
chondroitinase abc
crystallizable
sds
measurement
Prior art date
Application number
DK93304979T
Other languages
Danish (da)
English (en)
Inventor
Nobukazu Hashimoto
Akio Hamai
Hideo Mochizuki
Shinichi Ishikawa
Misako Torikai
Isao Miyachi
Original Assignee
Seikagaku Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP31098092A external-priority patent/JP3895782B2/ja
Application filed by Seikagaku Kogyo Co Ltd filed Critical Seikagaku Kogyo Co Ltd
Application granted granted Critical
Publication of DK0576294T3 publication Critical patent/DK0576294T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/2063—Proteins, e.g. gelatin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02004—Chondroitin ABC lyase (4.2.2.4), i.e. chondroitinase
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03C—PHOTOSENSITIVE MATERIALS FOR PHOTOGRAPHIC PURPOSES; PHOTOGRAPHIC PROCESSES, e.g. CINE, X-RAY, COLOUR, STEREO-PHOTOGRAPHIC PROCESSES; AUXILIARY PROCESSES IN PHOTOGRAPHY
    • G03C1/00—Photosensitive materials
    • G03C1/005—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein
    • G03C1/04—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein with macromolecular additives; with layer-forming substances
    • G03C1/047—Proteins, e.g. gelatine derivatives; Hydrolysis or extraction products of proteins
    • G03C2001/0471—Isoelectric point of gelatine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/873—Proteus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Analytical Chemistry (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
DK93304979T 1992-06-26 1993-06-25 Chondroitinase, fremgangsmåde til fremstilling af chondroitinase og farmaceutisk præparat indeholdende chondroitinase DK0576294T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP19288292 1992-06-26
JP31098092A JP3895782B2 (ja) 1992-10-26 1992-10-26 コンドロイチナーゼ組成物およびそれを含有する注射用製剤

Publications (1)

Publication Number Publication Date
DK0576294T3 true DK0576294T3 (da) 2000-10-09

Family

ID=26507572

Family Applications (1)

Application Number Title Priority Date Filing Date
DK93304979T DK0576294T3 (da) 1992-06-26 1993-06-25 Chondroitinase, fremgangsmåde til fremstilling af chondroitinase og farmaceutisk præparat indeholdende chondroitinase

Country Status (10)

Country Link
US (3) US5496718A (de)
EP (1) EP0576294B1 (de)
KR (1) KR100280779B1 (de)
AT (1) ATE192486T1 (de)
CA (1) CA2099138C (de)
DE (1) DE69328519T2 (de)
DK (1) DK0576294T3 (de)
ES (1) ES2146600T3 (de)
RU (1) RU2144833C1 (de)
SG (1) SG50677A1 (de)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
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US5496718A (en) * 1992-06-26 1996-03-05 Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) Chondroitinase ABC isolated from proteus vulgaris ATCC 6896
JP3419811B2 (ja) * 1993-02-24 2003-06-23 マルハ株式会社 コンドロイチナーゼ遺伝子
US7008783B1 (en) 1992-09-22 2006-03-07 Maruha Corporation Gene encoding chondroitinase ABC and uses therefor
WO1994025567A1 (en) * 1993-04-23 1994-11-10 American Cyanamid Company CLONING AND EXPRESSION OF THE CHONDROITINASE I AND II GENES FROM $i(P. VULGARIS)
AU1176595A (en) * 1993-11-12 1995-05-29 International Technology Management Associates, Ltd. Methods of repairing connective tissues
JPH10500843A (ja) * 1994-04-22 1998-01-27 アメリカン・サイアナミド・カンパニー コンドロイチナーゼｉおよびｉｉ、その製造法および用途
US5498536A (en) * 1994-04-22 1996-03-12 American Cyanamid Company Chondroitinase II from Proteus vulgaris
US5525500A (en) * 1994-04-22 1996-06-11 American Cyanamid Company Chromatographic process for the copurification of chondroitinase I and II proteins from Proteus vulgaris
US5888798A (en) * 1995-06-07 1999-03-30 American Cyanamid Company Chondroitinase I and chondroitinase II producing mutants of P. vulgaris
EP0747469A1 (de) 1995-06-08 1996-12-11 The Procter & Gamble Company Chondroitinase enthaltende Reinigungszusammensetzungen
EP0875253B1 (de) * 1997-05-02 2005-04-06 Seikagaku Corporation Chondroitinase enthaltende Zusammensetzungen
JPH1149698A (ja) 1997-07-31 1999-02-23 Santen Pharmaceut Co Ltd 安定性を向上させたラクトフェリンの水性製剤
AU735216B2 (en) * 1997-08-22 2001-07-05 Seikagaku Corporation Therapeutic agent for herniated intervertebral disc
WO2001017542A1 (fr) * 1999-09-08 2001-03-15 Chugai Seiyaku Kabushiki Kaisha Preparation de solution de proteines et procede de stabilisation associe
JP4252892B2 (ja) 2001-06-01 2009-04-08 三菱製紙株式会社 全熱交換素子用紙
MXPA04001334A (es) 2001-08-13 2004-05-05 Univ Florida Materiales y metodos para promover la reparacion de tejido nervioso.
GB0205022D0 (en) 2002-03-04 2002-04-17 Univ Cambridge Tech Materials and methods for the treatment of cns damage
EP1575548B1 (de) 2002-05-04 2011-03-09 Acorda Therapeutics, Inc. Zusammensetzungen und verfahren zur förderung des neuronalen wachstums
DE60334220D1 (de) * 2002-06-03 2010-10-28 Massachusetts Inst Technology Rationell konstruierte, von chondroitinase b abgeleitete lyasen
ATE508197T1 (de) * 2002-08-15 2011-05-15 Acorda Therapeutics Inc Chimärisches protein
EP2298874A1 (de) * 2002-12-16 2011-03-23 Halozyme, Inc. Humanes Chondrotinaseglykoprotein (CHASEGP), Verfahren zu dessen Herstellung und pharmazeutische Zusammensetzungen, die dieses umfassen
US20060104968A1 (en) * 2003-03-05 2006-05-18 Halozyme, Inc. Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminogly ycanases
US7871607B2 (en) 2003-03-05 2011-01-18 Halozyme, Inc. Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases
SG177008A1 (en) 2003-03-05 2012-01-30 Halozyme Inc Soluble hyaluronidase glycoprotein (shasegp), process for preparing the same, uses and pharmaceutical compositions comprising thereof
US20090123367A1 (en) * 2003-03-05 2009-05-14 Delfmems Soluble Glycosaminoglycanases and Methods of Preparing and Using Soluble Glycosaminoglycanases
US7959914B2 (en) * 2003-05-16 2011-06-14 Acorda Therapeutics, Inc. Methods of reducing extravasation of inflammatory cells
ES2616749T3 (es) 2003-05-16 2017-06-14 Acorda Therapeutics, Inc. Mutantes degradantes del proteoglucano para el tratamiento del SNC
AU2004241088B2 (en) * 2003-05-16 2010-08-05 Acorda Therapeutics, Inc. Compositions and methods for the treatment of CNS injuries
GB0403247D0 (en) 2004-02-13 2004-03-17 Tillotts Pharma Ag A pharmaceutical composition
EP1737954A2 (de) * 2004-03-10 2007-01-03 The Massachusetts Institute Of Technology Rekombinante chondroitinase abc i und verwendungen davon
WO2005112986A2 (en) * 2004-05-18 2005-12-01 Acorda Therapeutics, Inc. Purifying chondroitinase and stable formulations thereof
WO2005122734A2 (en) * 2004-06-14 2005-12-29 The Research Foundation Of State University Of New York Nanosphere/microsphere delivery system for the treatment of spinal cord injury
US20060110788A1 (en) * 2004-10-01 2006-05-25 Invitrogen Corporation Feeding buffers, systems, and methods for in vitro synthesis of biomolecules
JP5189985B2 (ja) 2005-09-26 2013-04-24 アコーダ セラピューティクス、インク． コンドロイチナーゼａｂｃｉ変異体を用いた組成物およびその使用方法
WO2007063691A1 (ja) 2005-11-29 2007-06-07 Sanyo Chemical Industries, Ltd. 溶菌剤
JP5391069B2 (ja) 2006-10-10 2014-01-15 アコーダ セラピューティクス、インク． コンドロイチナーゼａｂｃｉ変異体を使用する組成物及び方法
US20110059508A1 (en) 2007-06-29 2011-03-10 National University Corporation Nagoya University Improving agent for dysfunction due to neuropathy and rho kinase activation inhibitor
US20090286289A1 (en) * 2008-03-10 2009-11-19 Pang Danny Z Production of Hyaluronate Unsaturated Disaccharides and its Application
CA2821244C (en) * 2010-12-13 2016-08-09 Seikagaku Corporation Chondroitinase abc for disk herniation
CN106687129B (zh) 2014-09-27 2021-07-20 大日本住友制药株式会社 注射用药物组合物
US9796970B1 (en) 2017-04-24 2017-10-24 Advantek Serum Laboratories Ltd. Production of high purity chondroitinase ABC
WO2019167983A1 (ja) 2018-02-28 2019-09-06 生化学工業株式会社 医薬組成物、包装体及びその製造方法
CN114409731B (zh) * 2022-01-05 2023-08-11 浙江省农业科学院 同时具有激活乙醇脱氢酶和乙醛脱氢酶活性的两种多肽

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3549500A (en) * 1967-07-22 1970-12-22 Seikagaku Kogyo Co Ltd Novel chondroitinase and method of its recovery
JPS4832351B1 (de) * 1968-06-12 1973-10-05
JPS4713704Y1 (de) * 1971-09-29 1972-05-18
JPS6031478B2 (ja) * 1978-02-10 1985-07-22 生化学工業株式会社 純コンドロイチナ−ゼｂの製造法
JPS6031479B2 (ja) * 1978-02-10 1985-07-22 生化学工業株式会社 純コンドロイチナ−ゼｃの製造法
DE2962521D1 (en) * 1978-09-29 1982-05-27 Mitsubishi Chem Ind Process for the purification of long-chain acyl-coenzyme-a synthetase and the enzyme, acyl-coa synthetase, purified thereby
CA1221328A (en) * 1983-04-21 1987-05-05 Robert K. Scopes Enzyme extraction and purification processes
US4696816A (en) * 1985-11-07 1987-09-29 Brown Mark D Method for treating intervertebral disc displacement with enzymes
JPS62122588A (ja) * 1985-11-22 1987-06-03 Taiyo Fishery Co Ltd 純コンドロイチナ−ゼの製造法
JP2726275B2 (ja) * 1988-08-24 1998-03-11 生化学工業株式会社 グリコサミノグリカン分解酵素の精製法
JP2919496B2 (ja) * 1989-08-24 1999-07-12 生化学工業株式会社 新規コンドロイチン硫酸分解酵素、それらを産生する微生物並びにそれらの製法
JP3140797B2 (ja) * 1991-04-26 2001-03-05 生化学工業株式会社 安定化コンドロイチナーゼａｂｃ、その保存方法及び治療剤
US5496718A (en) * 1992-06-26 1996-03-05 Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) Chondroitinase ABC isolated from proteus vulgaris ATCC 6896

Also Published As

Publication number Publication date
US5496718A (en) 1996-03-05
AU4146493A (en) 1994-01-06
DE69328519D1 (de) 2000-06-08
CA2099138A1 (en) 1993-12-27
ES2146600T3 (es) 2000-08-16
US5763205A (en) 1998-06-09
EP0576294B1 (de) 2000-05-03
AU669528B2 (en) 1996-06-13
KR100280779B1 (ko) 2001-02-01
CA2099138C (en) 1998-09-22
RU2144833C1 (ru) 2000-01-27
EP0576294A2 (de) 1993-12-29
EP0576294A3 (en) 1994-08-17
KR940005793A (ko) 1994-03-22
SG50677A1 (en) 1998-07-20
DE69328519T2 (de) 2000-09-14
US6184023B1 (en) 2001-02-06
ATE192486T1 (de) 2000-05-15

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