DK0576294T3 - Chondroitinase, fremgangsmåde til fremstilling af chondroitinase og farmaceutisk præparat indeholdende chondroitinase - Google Patents

Chondroitinase, fremgangsmåde til fremstilling af chondroitinase og farmaceutisk præparat indeholdende chondroitinase

Info

Publication number
DK0576294T3
DK0576294T3 DK93304979T DK93304979T DK0576294T3 DK 0576294 T3 DK0576294 T3 DK 0576294T3 DK 93304979 T DK93304979 T DK 93304979T DK 93304979 T DK93304979 T DK 93304979T DK 0576294 T3 DK0576294 T3 DK 0576294T3
Authority
DK
Denmark
Prior art keywords
chondroitinase
chondroitinase abc
crystallizable
sds
measurement
Prior art date
Application number
DK93304979T
Other languages
English (en)
Inventor
Nobukazu Hashimoto
Akio Hamai
Hideo Mochizuki
Shinichi Ishikawa
Misako Torikai
Isao Miyachi
Original Assignee
Seikagaku Kogyo Co Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP31098092A external-priority patent/JP3895782B2/ja
Application filed by Seikagaku Kogyo Co Ltd filed Critical Seikagaku Kogyo Co Ltd
Application granted granted Critical
Publication of DK0576294T3 publication Critical patent/DK0576294T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/88—Lyases (4.)
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K9/00—Medicinal preparations characterised by special physical form
    • A61K9/20—Pills, tablets, discs, rods
    • A61K9/2004—Excipients; Inactive ingredients
    • A61K9/2022—Organic macromolecular compounds
    • A61K9/2063—Proteins, e.g. gelatin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/18—Ion-exchange chromatography
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K38/00—Medicinal preparations containing peptides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y402/00—Carbon-oxygen lyases (4.2)
    • C12Y402/02—Carbon-oxygen lyases (4.2) acting on polysaccharides (4.2.2)
    • C12Y402/02004—Chondroitin ABC lyase (4.2.2.4), i.e. chondroitinase
    • G—PHYSICS
    • G03—PHOTOGRAPHY; CINEMATOGRAPHY; ANALOGOUS TECHNIQUES USING WAVES OTHER THAN OPTICAL WAVES; ELECTROGRAPHY; HOLOGRAPHY
    • G03C—PHOTOSENSITIVE MATERIALS FOR PHOTOGRAPHIC PURPOSES; PHOTOGRAPHIC PROCESSES, e.g. CINE, X-RAY, COLOUR, STEREO-PHOTOGRAPHIC PROCESSES; AUXILIARY PROCESSES IN PHOTOGRAPHY
    • G03C1/00—Photosensitive materials
    • G03C1/005—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein
    • G03C1/04—Silver halide emulsions; Preparation thereof; Physical treatment thereof; Incorporation of additives therein with macromolecular additives; with layer-forming substances
    • G03C1/047—Proteins, e.g. gelatine derivatives; Hydrolysis or extraction products of proteins
    • G03C2001/0471—Isoelectric point of gelatine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S435/00—Chemistry: molecular biology and microbiology
    • Y10S435/8215—Microorganisms
    • Y10S435/822—Microorganisms using bacteria or actinomycetales
    • Y10S435/873—Proteus

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Biophysics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicinal Preparation (AREA)
DK93304979T 1992-06-26 1993-06-25 Chondroitinase, fremgangsmåde til fremstilling af chondroitinase og farmaceutisk præparat indeholdende chondroitinase DK0576294T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP19288292 1992-06-26
JP31098092A JP3895782B2 (ja) 1992-10-26 1992-10-26 コンドロイチナーゼ組成物およびそれを含有する注射用製剤

Publications (1)

Publication Number Publication Date
DK0576294T3 true DK0576294T3 (da) 2000-10-09

Family

ID=26507572

Family Applications (1)

Application Number Title Priority Date Filing Date
DK93304979T DK0576294T3 (da) 1992-06-26 1993-06-25 Chondroitinase, fremgangsmåde til fremstilling af chondroitinase og farmaceutisk præparat indeholdende chondroitinase

Country Status (10)

Country Link
US (3) US5496718A (da)
EP (1) EP0576294B1 (da)
KR (1) KR100280779B1 (da)
AT (1) ATE192486T1 (da)
CA (1) CA2099138C (da)
DE (1) DE69328519T2 (da)
DK (1) DK0576294T3 (da)
ES (1) ES2146600T3 (da)
RU (1) RU2144833C1 (da)
SG (1) SG50677A1 (da)

Families Citing this family (43)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5496718A (en) * 1992-06-26 1996-03-05 Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) Chondroitinase ABC isolated from proteus vulgaris ATCC 6896
JP3419811B2 (ja) * 1993-02-24 2003-06-23 マルハ株式会社 コンドロイチナーゼ遺伝子
US7008783B1 (en) 1992-09-22 2006-03-07 Maruha Corporation Gene encoding chondroitinase ABC and uses therefor
AU697156B2 (en) * 1993-04-23 1998-10-01 American Cyanamid Company Cloning and expression of the chondroitinase I and II genes from (p. vulgaris)
WO1995013091A1 (en) * 1993-11-12 1995-05-18 International Technology Management Associates, Ltd. Methods of repairing connective tissues
US5498536A (en) * 1994-04-22 1996-03-12 American Cyanamid Company Chondroitinase II from Proteus vulgaris
US5525500A (en) * 1994-04-22 1996-06-11 American Cyanamid Company Chromatographic process for the copurification of chondroitinase I and II proteins from Proteus vulgaris
EP0756636A4 (en) * 1994-04-22 1997-12-17 American Cyanamid Co CHONDROITINASES I AND II, PROCESSES FOR THEIR PREPARATION AND THEIR USE
US5888798A (en) * 1995-06-07 1999-03-30 American Cyanamid Company Chondroitinase I and chondroitinase II producing mutants of P. vulgaris
EP0747469A1 (en) 1995-06-08 1996-12-11 The Procter & Gamble Company Cleaning compositions comprising chondroitinase
EP0875253B1 (en) * 1997-05-02 2005-04-06 Seikagaku Corporation Chondroitinase compositions
JPH1149698A (ja) 1997-07-31 1999-02-23 Santen Pharmaceut Co Ltd 安定性を向上させたラクトフェリンの水性製剤
US6063378A (en) * 1997-08-22 2000-05-16 Seikagaku Corporation Therapeutic agent for herniated intervertebral disc
ATE389414T1 (de) * 1999-09-08 2008-04-15 Chugai Pharmaceutical Co Ltd Stabile proteinlösung abgefüllt in einem behältnis aus hydrophobem harz und eine methode zur stabilisierung derselben
KR100520722B1 (ko) 2001-06-01 2005-10-11 미쓰비시 세이시 가부시키가이샤 전(全)열교환 소자용지
CA2455827C (en) 2001-08-13 2015-06-09 University Of Florida Research Foundation, Inc. Materials and methods for nerve grafting comprising degrading chondroitin sulfate proteoglycan
GB0205022D0 (en) 2002-03-04 2002-04-17 Univ Cambridge Tech Materials and methods for the treatment of cns damage
US8183350B2 (en) * 2002-05-04 2012-05-22 Acorda Therapeutics, Inc. Compositions and methods for promoting neuronal outgrowth
EP1532241B1 (en) * 2002-06-03 2010-09-15 Massachusetts Institute Of Technology Rationally designed polysaccharide lyases derived from chondroitinase b
US20060153827A1 (en) * 2002-08-15 2006-07-13 Gruskin Elliott A Chimeric protein
EP2298874A1 (en) * 2002-12-16 2011-03-23 Halozyme, Inc. Human chondroitinase glycoprotein (CHASEGP), process for preparing the same, and pharmaceutical compositions comprising thereof
US20090123367A1 (en) * 2003-03-05 2009-05-14 Delfmems Soluble Glycosaminoglycanases and Methods of Preparing and Using Soluble Glycosaminoglycanases
US7871607B2 (en) * 2003-03-05 2011-01-18 Halozyme, Inc. Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminoglycanases
US20060104968A1 (en) * 2003-03-05 2006-05-18 Halozyme, Inc. Soluble glycosaminoglycanases and methods of preparing and using soluble glycosaminogly ycanases
PL2405015T3 (pl) 2003-03-05 2016-09-30 Rozpuszczalna glikoproteina o aktywności hialuronizady (sHASEGP), sposób jej wytwarzania, jej zastosowania i zawierające ją kompozycje farmaceutyczne
US7959914B2 (en) 2003-05-16 2011-06-14 Acorda Therapeutics, Inc. Methods of reducing extravasation of inflammatory cells
ES2373039T3 (es) * 2003-05-16 2012-01-30 Acorda Therapeutics, Inc. Composiciones y métodos para el tratamiento de lesiones del cns.
ES2636653T3 (es) 2003-05-16 2017-10-06 Acorda Therapeutics, Inc. Mutantes degradantes del proteoglucano para el tratamiento del SNC
GB0403247D0 (en) 2004-02-13 2004-03-17 Tillotts Pharma Ag A pharmaceutical composition
WO2005087920A2 (en) * 2004-03-10 2005-09-22 Massachusetts Institute Of Technology Recombinant chondroitinase abc i and uses thereof
WO2005112986A2 (en) * 2004-05-18 2005-12-01 Acorda Therapeutics, Inc. Purifying chondroitinase and stable formulations thereof
WO2005122734A2 (en) * 2004-06-14 2005-12-29 The Research Foundation Of State University Of New York Nanosphere/microsphere delivery system for the treatment of spinal cord injury
EP1848812A4 (en) * 2004-10-01 2011-11-09 Life Technologies Corp SUPPLY BUFFERS, SYSTEMS AND METHODS FOR THE IN VITRO SYNTHESIS OF BIOMOLECULES
WO2007038548A2 (en) 2005-09-26 2007-04-05 Acorda Therapeutics, Inc. Compositions and methods of using chondroitinase abci mutants
US8003369B2 (en) 2005-11-29 2011-08-23 Sanyo Chemical Industries, Ltd. Bacteriolytic agent
AU2007307736C1 (en) 2006-10-10 2014-04-10 Acorda Therapeutics, Inc. Compositions and methods of using chondroitinase ABCI mutants
JP5341756B2 (ja) 2007-06-29 2013-11-13 国立大学法人名古屋大学 神経障害に基づく機能不全の改善剤およびＲｈｏキナーゼ活性化抑制剤
US20090286289A1 (en) * 2008-03-10 2009-11-19 Pang Danny Z Production of Hyaluronate Unsaturated Disaccharides and its Application
RU2690185C2 (ru) * 2010-12-13 2019-05-31 Сейкагаку Корпорейшн Терапевтическое средство от грыжи межпозвоночного диска
ES2916834T3 (es) 2014-09-27 2022-07-06 Sumitomo Pharma Co Ltd Composición farmacéutica para inyección
US9796970B1 (en) 2017-04-24 2017-10-24 Advantek Serum Laboratories Ltd. Production of high purity chondroitinase ABC
CA3089180A1 (en) 2018-02-28 2019-09-06 Seikagaku Corporation Pharmaceutical composition, package, and method for producing the same
CN114409731B (zh) * 2022-01-05 2023-08-11 浙江省农业科学院 同时具有激活乙醇脱氢酶和乙醛脱氢酶活性的两种多肽

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3549500A (en) * 1967-07-22 1970-12-22 Seikagaku Kogyo Co Ltd Novel chondroitinase and method of its recovery
JPS4832351B1 (da) * 1968-06-12 1973-10-05
JPS4713704Y1 (da) * 1971-09-29 1972-05-18
JPS6031479B2 (ja) * 1978-02-10 1985-07-22 生化学工業株式会社 純コンドロイチナ−ゼｃの製造法
JPS6031478B2 (ja) * 1978-02-10 1985-07-22 生化学工業株式会社 純コンドロイチナ−ゼｂの製造法
DK151634C (da) * 1978-09-29 1988-06-20 Mitsubishi Chem Ind Fremgangsmaade til rensning af langkaedet acyl-coenzym-a-syntetase
EP0139705A1 (en) * 1983-04-21 1985-05-08 La Trobe University Enzyme extraction and purification processes
US4696816A (en) * 1985-11-07 1987-09-29 Brown Mark D Method for treating intervertebral disc displacement with enzymes
JPS62122588A (ja) * 1985-11-22 1987-06-03 Taiyo Fishery Co Ltd 純コンドロイチナ−ゼの製造法
JP2726275B2 (ja) * 1988-08-24 1998-03-11 生化学工業株式会社 グリコサミノグリカン分解酵素の精製法
JP2919496B2 (ja) * 1989-08-24 1999-07-12 生化学工業株式会社 新規コンドロイチン硫酸分解酵素、それらを産生する微生物並びにそれらの製法
JP3140797B2 (ja) * 1991-04-26 2001-03-05 生化学工業株式会社 安定化コンドロイチナーゼａｂｃ、その保存方法及び治療剤
US5496718A (en) * 1992-06-26 1996-03-05 Seikagaku Kogyo Kabushiki Kaisha (Seikagaku Corporation) Chondroitinase ABC isolated from proteus vulgaris ATCC 6896

Also Published As

Publication number Publication date
AU669528B2 (en) 1996-06-13
ES2146600T3 (es) 2000-08-16
KR940005793A (ko) 1994-03-22
AU4146493A (en) 1994-01-06
DE69328519D1 (de) 2000-06-08
CA2099138A1 (en) 1993-12-27
US6184023B1 (en) 2001-02-06
ATE192486T1 (de) 2000-05-15
EP0576294A2 (en) 1993-12-29
RU2144833C1 (ru) 2000-01-27
US5763205A (en) 1998-06-09
DE69328519T2 (de) 2000-09-14
SG50677A1 (en) 1998-07-20
KR100280779B1 (ko) 2001-02-01
CA2099138C (en) 1998-09-22
US5496718A (en) 1996-03-05
EP0576294A3 (en) 1994-08-17
EP0576294B1 (en) 2000-05-03

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