RU93046317A - Хиндроитиназа, способ ее получения и содержание ее фармацевтические композиции - Google Patents

Хиндроитиназа, способ ее получения и содержание ее фармацевтические композиции

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Publication number
RU93046317A
RU93046317A RU93046317/14A RU93046317A RU93046317A RU 93046317 A RU93046317 A RU 93046317A RU 93046317/14 A RU93046317/14 A RU 93046317/14A RU 93046317 A RU93046317 A RU 93046317A RU 93046317 A RU93046317 A RU 93046317A
Authority
RU
Russia
Prior art keywords
chondroitinase abc
amino acid
terminal amino
chindroitinase
contents
Prior art date
Application number
RU93046317/14A
Other languages
English (en)
Other versions
RU2144833C1 (ru
Inventor
Хосимота Нобуказу
Матизуки Хидео
Хамаи Акио
Исикава Синити
Торикаи Мисако
Мияти Исао
Original Assignee
Сейкагаку Когио Кабусики Кайся (Сейкагаку Корпорейшн)
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from JP31098092A external-priority patent/JP3895782B2/ja
Application filed by Сейкагаку Когио Кабусики Кайся (Сейкагаку Корпорейшн) filed Critical Сейкагаку Когио Кабусики Кайся (Сейкагаку Корпорейшн)
Publication of RU93046317A publication Critical patent/RU93046317A/ru
Application granted granted Critical
Publication of RU2144833C1 publication Critical patent/RU2144833C1/ru

Links

Claims (1)

  1. Кристаллизующаяся очищенная хондроитиназа ABC с молекулярной массой около 100000 получена методом электрофореза с использованием додецилсульфата натрия - полиакриламидного геля (SDS - PAGE) и методом гельпроникающей хроматографии содержит аланин в качестве N -концевой аминокислоты и пролин в качестве C - концевой аминокислоты. Способ очистки кристаллизующейся очищенной хондроитиназы ABC включает удаление нуклеиновой кислоты раствором поверхностно-активного вещества из экстракта, полученного из клеток микроорганизмов, продуцирующих хондроитиназу ABC, и хроматографирование при градиентном концентрированном элюировании с применением слабой катионо-обменной смолы или сильной катионо-обменной смолы. Композиция включает зондроитиназу и сывороточный альбумин, желатин или неионное поверхностно-активное вещество.
RU93046317A 1992-06-26 1993-06-25 Хондроитиназа, способ ее получения и включающие ее фармацевтические композиции RU2144833C1 (ru)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
JP19288292 1992-06-26
JP192882/1992 1992-06-26
JP310980/1992 1992-10-26
JP31098092A JP3895782B2 (ja) 1992-10-26 1992-10-26 コンドロイチナーゼ組成物およびそれを含有する注射用製剤

Publications (2)

Publication Number Publication Date
RU93046317A true RU93046317A (ru) 1996-05-20
RU2144833C1 RU2144833C1 (ru) 2000-01-27

Family

ID=26507572

Family Applications (1)

Application Number Title Priority Date Filing Date
RU93046317A RU2144833C1 (ru) 1992-06-26 1993-06-25 Хондроитиназа, способ ее получения и включающие ее фармацевтические композиции

Country Status (10)

Country Link
US (3) US5496718A (ru)
EP (1) EP0576294B1 (ru)
KR (1) KR100280779B1 (ru)
AT (1) ATE192486T1 (ru)
CA (1) CA2099138C (ru)
DE (1) DE69328519T2 (ru)
DK (1) DK0576294T3 (ru)
ES (1) ES2146600T3 (ru)
RU (1) RU2144833C1 (ru)
SG (1) SG50677A1 (ru)

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