DE69938512T2 - Optischer autofokus zur verwendung bei mikrotiterplatten - Google Patents

Optischer autofokus zur verwendung bei mikrotiterplatten Download PDF

Info

Publication number
DE69938512T2
DE69938512T2 DE69938512T DE69938512T DE69938512T2 DE 69938512 T2 DE69938512 T2 DE 69938512T2 DE 69938512 T DE69938512 T DE 69938512T DE 69938512 T DE69938512 T DE 69938512T DE 69938512 T2 DE69938512 T2 DE 69938512T2
Authority
DE
Germany
Prior art keywords
light
well
microplate
focus
fluorescence
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69938512T
Other languages
German (de)
English (en)
Other versions
DE69938512D1 (de
Inventor
Bala S. Los Altos Hills MANIAN
David M. Oakland HEFFELFINGER
Edward M. Redwood City GOLDBERG
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
BD Biosciences Systems and Reagents Inc
Original Assignee
BD Biosciences Systems and Reagents Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by BD Biosciences Systems and Reagents Inc filed Critical BD Biosciences Systems and Reagents Inc
Application granted granted Critical
Publication of DE69938512D1 publication Critical patent/DE69938512D1/de
Publication of DE69938512T2 publication Critical patent/DE69938512T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/34Microscope slides, e.g. mounting specimens on microscope slides
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64Fluorescence; Phosphorescence
    • G01N21/645Specially adapted constructive features of fluorimeters
    • G01N21/6452Individual samples arranged in a regular 2D-array, e.g. multiwell plates
    • GPHYSICS
    • G02OPTICS
    • G02BOPTICAL ELEMENTS, SYSTEMS OR APPARATUS
    • G02B21/00Microscopes
    • G02B21/24Base structure
    • G02B21/241Devices for focusing

Landscapes

  • Physics & Mathematics (AREA)
  • Chemical & Material Sciences (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Optics & Photonics (AREA)
  • Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Health & Medical Sciences (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
  • Optical Measuring Cells (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Automatic Focus Adjustment (AREA)
  • Length Measuring Devices By Optical Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
DE69938512T 1999-02-05 1999-05-03 Optischer autofokus zur verwendung bei mikrotiterplatten Expired - Lifetime DE69938512T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US245782 1999-02-05
US09/245,782 US6130745A (en) 1999-01-07 1999-02-05 Optical autofocus for use with microtiter plates
PCT/US1999/009699 WO2000046590A1 (en) 1999-02-05 1999-05-03 Optical autofocus for use with microtiter plates

Publications (2)

Publication Number Publication Date
DE69938512D1 DE69938512D1 (de) 2008-05-21
DE69938512T2 true DE69938512T2 (de) 2009-04-30

Family

ID=22928053

Family Applications (2)

Application Number Title Priority Date Filing Date
DE69938512T Expired - Lifetime DE69938512T2 (de) 1999-02-05 1999-05-03 Optischer autofokus zur verwendung bei mikrotiterplatten
DE69941236T Expired - Lifetime DE69941236D1 (de) 1999-02-05 1999-05-03 Optischer Autofokus zur Verwendung mit Mikrotiter-Platten

Family Applications After (1)

Application Number Title Priority Date Filing Date
DE69941236T Expired - Lifetime DE69941236D1 (de) 1999-02-05 1999-05-03 Optischer Autofokus zur Verwendung mit Mikrotiter-Platten

Country Status (5)

Country Link
US (2) US6130745A (https=)
EP (2) EP1942333B1 (https=)
JP (1) JP4521121B2 (https=)
DE (2) DE69938512T2 (https=)
WO (1) WO2000046590A1 (https=)

Families Citing this family (72)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20030030797A1 (en) * 1999-09-24 2003-02-13 Henry Palladino Solid state fluorescence and absorption spectroscopy
US6586257B1 (en) * 1999-10-12 2003-07-01 Vertex Pharmaceuticals Incorporated Multiwell scanner and scanning method
US6814933B2 (en) 2000-09-19 2004-11-09 Aurora Biosciences Corporation Multiwell scanner and scanning method
FR2801977B1 (fr) * 1999-12-02 2002-05-17 Commissariat Energie Atomique Amplification d'un signal de fluorescence emis par un echantillon surfacique
WO2002063367A1 (en) * 2001-02-02 2002-08-15 Cellomics, Inc. Method for estimating the best initial focus position
FR2820828B1 (fr) * 2001-02-09 2003-05-02 Commissariat Energie Atomique Dispositif d'observation d'echantillons par fluorescence, notamment de facon sequentielle
US6979530B2 (en) * 2001-05-21 2005-12-27 Applera Corporation Peptide conjugates and fluorescence detection methods for intracellular caspase assay
DE10141544A1 (de) * 2001-08-24 2003-03-13 Eppendorf Ag Vorrichtung zur Behandlung von Flüssigkeiten und Verfahren zum Betreiben der Vorrichtung
US6750457B2 (en) * 2001-08-29 2004-06-15 Becton Dickinson And Company System for high throughput analysis
DE10157511A1 (de) * 2001-11-23 2003-06-12 Evotec Ag Verfahren und Vorrichtung zur Korrektur der Größe und/oder Form eines Messvolumens in einer chemischen und/oder biologischen Probe
JP4497923B2 (ja) * 2001-12-05 2010-07-07 ザ ジェイ. デビッド グラッドストーン インスティテューツ ロボット顕微鏡検査システム
FR2833718A1 (fr) * 2001-12-13 2003-06-20 Commissariat Energie Atomique Dispositif d'observation d'objets, notamment pour plaques de microtitration
US7205154B2 (en) * 2002-01-31 2007-04-17 Agilent Technologies, Inc. Calibrating array scanners
US6770892B2 (en) * 2002-02-28 2004-08-03 Agilent Technologies, Inc. Method and system for automated focus-distance determination for molecular array scanners
IL148664A0 (en) * 2002-03-13 2002-09-12 Yeda Res & Dev Auto-focusing method and device
WO2003095986A1 (en) 2002-05-14 2003-11-20 Amersham Biosciences Niagara, Inc. System and methods for rapid and automated screening of cells
US9157860B2 (en) * 2002-05-16 2015-10-13 Applied Biosystems, Llc Achromatic lens array
US6982166B2 (en) * 2002-05-16 2006-01-03 Applera Corporation Lens assembly for biological testing
JPWO2004027085A1 (ja) * 2002-09-05 2006-01-19 日東電工株式会社 固体表面の微生物試験用粘着シートおよびキット
JPWO2004022774A1 (ja) * 2002-09-05 2005-12-22 富士電機システムズ株式会社 微生物または細胞の検出方法
DE10244618A1 (de) * 2002-09-25 2004-04-08 Olympus Biosystems Gmbh Einrichtung und Verfahren zur optischen Objektuntersuchung oder/und Erfassung oder/und Untersuchung von Schichten
EP1585978A4 (en) * 2002-12-30 2009-12-16 Pelikan Technologies Inc METHOD AND APPARATUS FOR USING OPTICAL TECHNIQUES FOR MEASURING ANALYTE RATES
US20040150217A1 (en) * 2003-01-23 2004-08-05 Heffelfinger David M. Identifying indicia and focusing target
US7522762B2 (en) * 2003-04-16 2009-04-21 Inverness Medical-Biostar, Inc. Detection, resolution, and identification of arrayed elements
US6853454B1 (en) 2004-01-15 2005-02-08 Alpha Innotech Corporation Optical analysis systems
US20050157299A1 (en) * 2004-01-15 2005-07-21 Heffelfinger David M. Optical analysis systems
US6995901B2 (en) * 2004-01-15 2006-02-07 Alpha Innotech Corporation Optical analysis systems
EP1760455A4 (en) * 2004-06-21 2016-12-21 Olympus Corp MEASURING DEVICE
FI20055043A0 (fi) * 2005-01-31 2005-01-31 Wallac Oy Sovitelma mikrotiterkaivojen kuvausjärjestelmässä
JP4701781B2 (ja) * 2005-03-28 2011-06-15 カシオ計算機株式会社 生体高分子分析装置及び生体高分子分析方法
US7718131B2 (en) 2005-07-06 2010-05-18 Genetix Limited Methods and apparatus for imaging and processing of samples in biological sample containers
EP1930717B1 (en) * 2005-09-29 2019-06-19 Olympus Corporation Focal position determining method and apparatus
FR2898363B1 (fr) * 2006-03-10 2008-06-13 Biofilm Control Sarl Prodede de releves d'images, notamment pour l'etude du developpement d'un biofilm dans un milieu de culture
CN101438147B (zh) 2006-05-31 2011-09-28 奥林巴斯株式会社 生物试样摄像方法及生物试样摄像装置
JP2008267842A (ja) * 2007-04-17 2008-11-06 Yokogawa Electric Corp 生物観察容器並びにこれを用いる生物顕微鏡及び生物観察装置
JP5152622B2 (ja) * 2007-05-23 2013-02-27 横河電機株式会社 創薬スクリーニング装置及びこの装置を用いた創薬スクリーニング方法
US7646482B2 (en) * 2007-05-31 2010-01-12 Genetix Limited Methods and apparatus for optical analysis of samples in biological sample containers
US7535556B2 (en) * 2007-05-31 2009-05-19 Genetix Limited Methods and apparatus for optical analysis of samples in biological sample containers
EP2110696B1 (en) 2008-04-15 2013-10-16 Sensovation AG Method and apparatus for autofocus
FR2931966A1 (fr) * 2008-05-30 2009-12-04 Novacyt Procede d'analyse cellulaire d'un prelevement au moyen d'une plaque d'analyse virtuelle
DE202009003288U1 (de) 2009-03-11 2009-06-25 Sensovation Ag Autofokuseinrichtung
WO2010103389A1 (de) 2009-03-11 2010-09-16 Sensovation Ag Autofokusverfahren und autofokuseinrichtung
DE102009012293A1 (de) 2009-03-11 2010-09-16 Sensovation Ag Autofokusverfahren und Autofokuseinrichtung
JP5167194B2 (ja) * 2009-04-28 2013-03-21 株式会社日立エンジニアリング・アンド・サービス 微生物検査装置
US7952799B2 (en) * 2009-06-19 2011-05-31 Corning Incorporated Extreme broadband compact optical system with multiple fields of view
US8304704B2 (en) * 2009-07-27 2012-11-06 Sensovation Ag Method and apparatus for autofocus using a light source pattern and means for masking the light source pattern
US9360659B2 (en) * 2011-08-10 2016-06-07 Molecular Devices, Llc Method for presenting and evaluation of images of micro-titer plate properties
JP2012159854A (ja) * 2012-04-04 2012-08-23 Olympus Corp 倒立顕微鏡システム
EP2769204B1 (en) 2012-05-02 2016-02-17 Charles River Laboratories, Inc. Cell capture system and use thereof
EP2780707B1 (en) 2012-05-02 2016-02-24 Charles River Laboratories, Inc. Method of detecting viable cells in a cell sample
AU2013256174B2 (en) 2012-05-02 2017-03-02 Charles River Laboratories, Inc. Viability staining method
US20140168402A1 (en) * 2012-12-13 2014-06-19 Vala Sciences, Inc. Continuous-Scanning Image Acquisition in Automated Microscopy Using Reflective Autofocus
EP2932425B1 (en) 2012-12-14 2025-01-29 The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone Automated robotic microscopy systems
US9279750B2 (en) * 2013-03-15 2016-03-08 Iris International, Inc. Method and composition for staining and sample processing
JP6523245B2 (ja) 2013-03-15 2019-05-29 アイリス インターナショナル, インコーポレイテッド 血液試料における粒子分析のためのシース液システム及び方法
US9857361B2 (en) * 2013-03-15 2018-01-02 Iris International, Inc. Flowcell, sheath fluid, and autofocus systems and methods for particle analysis in urine samples
DE102013103971A1 (de) 2013-04-19 2014-11-06 Sensovation Ag Verfahren zum Erzeugen eines aus mehreren Teilbildern zusammengesetzten Gesamtbilds eines Objekts
FR3012131B1 (fr) * 2013-10-18 2018-01-19 Centre National De La Recherche Scientifique Supports amplificateurs de contraste pour l'observation d'un echantillon, leur procedes de fabrication et leurs utilisations
US10007102B2 (en) 2013-12-23 2018-06-26 Sakura Finetek U.S.A., Inc. Microscope with slide clamping assembly
JP6673198B2 (ja) * 2014-05-30 2020-03-25 株式会社ニコン 顕微鏡
US10941435B2 (en) 2016-06-23 2021-03-09 Life Technologies Corporation Methods and compositions for detecting or measuring caspases or apoptosis
EP3287829A1 (en) * 2016-08-25 2018-02-28 Deutsches Krebsforschungszentrum Method of and microscope with installation for focus stabilization
US11280803B2 (en) 2016-11-22 2022-03-22 Sakura Finetek U.S.A., Inc. Slide management system
ES2924275T3 (es) * 2017-01-19 2022-10-05 Otsuka Pharma Co Ltd Método de detección de posición y dispositivo de detección de posición para un chip sensor en un sistema óptico de detección de muestras
JP6853728B2 (ja) * 2017-04-28 2021-03-31 株式会社Screenホールディングス 試料容器およびこれを用いる撮像方法
JP7212028B2 (ja) 2017-07-13 2023-01-24 グライナー バイオ‐ワン ノース アメリカ,インコーポレイテッド 撮像用の培養プレート
CN108519332B (zh) * 2018-04-28 2019-12-03 南通市肺科医院(南通市第六人民医院) 全自动微孔板系统及用于该系统的计算机可读介质
WO2019244275A1 (ja) * 2018-06-20 2019-12-26 株式会社日立ハイテクノロジーズ 観察装置
GB201816687D0 (en) * 2018-10-12 2018-11-28 Clinspec Diagnostics Ltd Sample container
EP4251974A4 (en) * 2020-11-25 2024-11-20 Mabtech Ab APPARATUS AND METHOD FOR FOCUSING A LASER SCANNING CYTOMETER USING THE REFLECTION OF THE LASER
EP4198604B1 (en) * 2021-12-17 2025-10-29 Thermo Electron Scientific Instruments LLC Method and system for positioning a sample
WO2024101416A1 (ja) * 2022-11-10 2024-05-16 キヤノン株式会社 走査方法、走査システム及び位置情報取得方法

Family Cites Families (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI790692A7 (fi) * 1979-03-01 1980-09-02 Suovaniemi Finnpipette Mikrokyvettenhet
JPS5827755U (ja) * 1981-08-19 1983-02-22 オリンパス光学工業株式会社 マイクロプレ−ト
JPS5891164U (ja) * 1981-12-15 1983-06-20 オリンパス光学工業株式会社 粒子凝集反応容器
AU585033B2 (en) * 1986-07-04 1989-06-08 Tosoh Corporation Quantitative dispenser for a liquid
JPS63193350U (https=) * 1987-05-30 1988-12-13
US4844617A (en) * 1988-01-20 1989-07-04 Tencor Instruments Confocal measuring microscope with automatic focusing
JP2717730B2 (ja) * 1991-01-09 1998-02-25 富士写真フイルム株式会社 液面レベル検出装置
US5355215A (en) * 1992-09-30 1994-10-11 Environmental Research Institute Of Michigan Method and apparatus for quantitative fluorescence measurements
US5556764A (en) * 1993-02-17 1996-09-17 Biometric Imaging, Inc. Method and apparatus for cell counting and cell classification
US5547849A (en) * 1993-02-17 1996-08-20 Biometric Imaging, Inc. Apparatus and method for volumetric capillary cytometry
EP0695941B1 (en) * 1994-06-08 2002-07-31 Affymetrix, Inc. Method and apparatus for packaging a chip
JP2852190B2 (ja) * 1994-09-02 1999-01-27 浜松ホトニクス株式会社 微小位置測定装置
US5499097A (en) * 1994-09-19 1996-03-12 Neopath, Inc. Method and apparatus for checking automated optical system performance repeatability
IL111229A (en) * 1994-10-10 1998-06-15 Nova Measuring Instr Ltd Autofocusing microscope
US5589351A (en) * 1994-12-06 1996-12-31 Nps Pharmaceuticals, Inc. Fluorescence detection apparatus
US5548392A (en) * 1995-01-31 1996-08-20 Kabushikikaisha Wacom Optical position detector having scale pattern spot-illuminated to increase resolution
JPH08219812A (ja) * 1995-02-15 1996-08-30 Canon Inc 変位情報検出装置、変位情報検出用スケール及びこれを用いたドライブ制御装置
US5784152A (en) * 1995-03-16 1998-07-21 Bio-Rad Laboratories Tunable excitation and/or tunable detection microplate reader
US5776674A (en) * 1995-06-05 1998-07-07 Seq, Ltd Chemical biochemical and biological processing in thin films
US5764272A (en) * 1995-09-12 1998-06-09 Eastman Kodak Company Autofocus mechanism for laser imager
JP3291995B2 (ja) * 1995-09-13 2002-06-17 富士通株式会社 変動液面高さ測定装置及び方法
US5774214A (en) * 1996-12-12 1998-06-30 Photometrics, Ltd. Multi-mode imaging apparatus for radiation-emitting or absorbing samples
WO1998038490A1 (en) * 1997-02-27 1998-09-03 Cellomics, Inc. A system for cell-based screening
US6071748A (en) * 1997-07-16 2000-06-06 Ljl Biosystems, Inc. Light detection device
US6081487A (en) * 1998-02-13 2000-06-27 International Business Machines Corporation System for creating, reading and writing on rotatable information storage media, an apparatus for controlling laser positioning
FR2784189B3 (fr) * 1998-10-05 2000-11-03 Commissariat Energie Atomique Biopuce et dispositif de lecture d'une biopuce comportant une pluralite de zones de reconnaissance moleculaire

Also Published As

Publication number Publication date
US6130745A (en) 2000-10-10
JP4521121B2 (ja) 2010-08-11
EP1942333A1 (en) 2008-07-09
EP1169632A4 (en) 2007-07-18
DE69941236D1 (de) 2009-09-17
EP1942333B1 (en) 2009-08-05
DE69938512D1 (de) 2008-05-21
EP1169632B1 (en) 2008-04-09
EP1169632A1 (en) 2002-01-09
WO2000046590A1 (en) 2000-08-10
US6441894B1 (en) 2002-08-27
JP2002541430A (ja) 2002-12-03

Similar Documents

Publication Publication Date Title
DE69938512T2 (de) Optischer autofokus zur verwendung bei mikrotiterplatten
DE69714915T2 (de) Verfahren zur Messung der Dicke und Brechungsindices der Schichtbauteile einer laminierten Struktur und Messgerät zu dessen Durchführung
DE69830529T2 (de) Oberflächen-Plasmonen-Sensor
EP1145066B1 (de) Positionierung des messvolumens in einem scanning-mikroskopischen verfahren
DE60133383T2 (de) Verfahren und Vorrichtung zur Oberflächenplasmonenmikroskopie
EP1354234B1 (de) Optisches system und verfahren zum anregen und messen von fluoreszenz an oder in mit fluoreszensfarbstoffen behandelten proben
DE60129920T2 (de) Interferenzmikroskop und Verfahren zum Bedienen eines Interferenzmikroskops
DE69816809T2 (de) Vorrichtung für fluoreszenznachweis
EP4325208B1 (de) Verfahren und vorrichtung zum erfassen von verlagerungen einer probe gegenüber einem objektiv
WO2008125204A1 (de) Verfahren und anordnung zum positionieren eines lichtblattes in der fokusebene einer detektionsoptik
EP1190236A1 (de) Sensorplatform und verfahren zur multianalytbestimmung
EP3561040B1 (de) Dispensiervorrichtung mit einem dispenser zum ausgeben einer wenigstens eine zelle und/oder wenigstens ein partikel enthaltenen flüssigkeit
EP1511989B1 (de) Fluoreszenzmesssystem
WO2009003714A2 (de) Vorrichtung und verfahren zur durchführung statischer und dynamischer streulichtmessungen in kleinen volumina
DE10100246A1 (de) Mikroskop und Verfahren zum Betreiben eines Mikroskops
EP3074808A1 (de) Lichtmikroskop mit innenfokussierendem objektiv und mikroskopieverfahren zum untersuchen mehrerer mikroskopischer objekte
DE60114656T2 (de) Chip-element für mikrochemische systeme und ein das chip-element verwendendes mikrochemisches system
DE19729245C1 (de) Spiegelobjektiv und dessen Verwendung
DE60130452T2 (de) Referenzvorrichtung zur Evaluierung der Funktion eines konfokalen Laserscanmikroskops, sowie Verfahren und System zur Durchführung der Evaluierung
DE10107210C1 (de) Mikroskop
DE19924259C2 (de) Vorrichtung und Verfahren zur Erfassung des Füllstandes eines Flüssigkeitsbehälters
EP1288635A2 (de) Vorrichtung zur Behandlung von Flüssigkeiten und Verfahren zum Betreiben der Vorrichtung
EP1198694B1 (de) Optischer sensor zur messung des abstands und der neigung einer fläche
EP1543369B1 (de) Autofokus-einrichtung und verfahren zur optischen untersuchung oder/und erfassung von schichten
DE69120253T2 (de) Apparat zur fluoreszenzanalyse

Legal Events

Date Code Title Description
8364 No opposition during term of opposition