DE69938512T2 - Optischer autofokus zur verwendung bei mikrotiterplatten - Google Patents
Optischer autofokus zur verwendung bei mikrotiterplatten Download PDFInfo
- Publication number
- DE69938512T2 DE69938512T2 DE69938512T DE69938512T DE69938512T2 DE 69938512 T2 DE69938512 T2 DE 69938512T2 DE 69938512 T DE69938512 T DE 69938512T DE 69938512 T DE69938512 T DE 69938512T DE 69938512 T2 DE69938512 T2 DE 69938512T2
- Authority
- DE
- Germany
- Prior art keywords
- light
- well
- microplate
- focus
- fluorescence
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 230000003287 optical effect Effects 0.000 title claims description 20
- 238000000034 method Methods 0.000 claims description 25
- 239000007788 liquid Substances 0.000 claims description 19
- 150000001875 compounds Chemical class 0.000 description 12
- 230000005540 biological transmission Effects 0.000 description 11
- 238000005259 measurement Methods 0.000 description 11
- 238000001514 detection method Methods 0.000 description 10
- 230000033001 locomotion Effects 0.000 description 10
- 239000000463 material Substances 0.000 description 9
- 238000012360 testing method Methods 0.000 description 9
- 230000007704 transition Effects 0.000 description 7
- 238000000576 coating method Methods 0.000 description 6
- 238000012546 transfer Methods 0.000 description 5
- 239000003153 chemical reaction reagent Substances 0.000 description 4
- 230000008602 contraction Effects 0.000 description 4
- 239000011521 glass Substances 0.000 description 4
- 230000008901 benefit Effects 0.000 description 3
- 239000011248 coating agent Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- XUIMIQQOPSSXEZ-UHFFFAOYSA-N Silicon Chemical compound [Si] XUIMIQQOPSSXEZ-UHFFFAOYSA-N 0.000 description 2
- 239000006117 anti-reflective coating Substances 0.000 description 2
- 239000012472 biological sample Substances 0.000 description 2
- 238000011161 development Methods 0.000 description 2
- 239000003814 drug Substances 0.000 description 2
- 238000001917 fluorescence detection Methods 0.000 description 2
- 230000005484 gravity Effects 0.000 description 2
- 230000007935 neutral effect Effects 0.000 description 2
- 239000000523 sample Substances 0.000 description 2
- 229910052710 silicon Inorganic materials 0.000 description 2
- 239000010703 silicon Substances 0.000 description 2
- 238000002835 absorbance Methods 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 238000003556 assay Methods 0.000 description 1
- 239000011324 bead Substances 0.000 description 1
- 230000001413 cellular effect Effects 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 230000001427 coherent effect Effects 0.000 description 1
- 238000003271 compound fluorescence assay Methods 0.000 description 1
- 230000007423 decrease Effects 0.000 description 1
- 230000008021 deposition Effects 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 229940079593 drug Drugs 0.000 description 1
- 229940000406 drug candidate Drugs 0.000 description 1
- 238000007876 drug discovery Methods 0.000 description 1
- 230000002996 emotional effect Effects 0.000 description 1
- 230000005284 excitation Effects 0.000 description 1
- 239000012530 fluid Substances 0.000 description 1
- 238000012921 fluorescence analysis Methods 0.000 description 1
- 238000007496 glass forming Methods 0.000 description 1
- 230000009477 glass transition Effects 0.000 description 1
- CPBQJMYROZQQJC-UHFFFAOYSA-N helium neon Chemical compound [He].[Ne] CPBQJMYROZQQJC-UHFFFAOYSA-N 0.000 description 1
- 238000005286 illumination Methods 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- ORUIBWPALBXDOA-UHFFFAOYSA-L magnesium fluoride Chemical compound [F-].[F-].[Mg+2] ORUIBWPALBXDOA-UHFFFAOYSA-L 0.000 description 1
- 229910001635 magnesium fluoride Inorganic materials 0.000 description 1
- 239000003550 marker Substances 0.000 description 1
- 239000011159 matrix material Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000010606 normalization Methods 0.000 description 1
- 210000001747 pupil Anatomy 0.000 description 1
- 238000011160 research Methods 0.000 description 1
- 230000004044 response Effects 0.000 description 1
- 238000012216 screening Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000013076 target substance Substances 0.000 description 1
- 238000010998 test method Methods 0.000 description 1
- BYMUNNMMXKDFEZ-UHFFFAOYSA-K trifluorolanthanum Chemical compound F[La](F)F BYMUNNMMXKDFEZ-UHFFFAOYSA-K 0.000 description 1
- 238000007738 vacuum evaporation Methods 0.000 description 1
Classifications
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/34—Microscope slides, e.g. mounting specimens on microscope slides
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/645—Specially adapted constructive features of fluorimeters
- G01N21/6452—Individual samples arranged in a regular 2D-array, e.g. multiwell plates
-
- G—PHYSICS
- G02—OPTICS
- G02B—OPTICAL ELEMENTS, SYSTEMS OR APPARATUS
- G02B21/00—Microscopes
- G02B21/24—Base structure
- G02B21/241—Devices for focusing
Landscapes
- Physics & Mathematics (AREA)
- Chemical & Material Sciences (AREA)
- Analytical Chemistry (AREA)
- General Physics & Mathematics (AREA)
- Optics & Photonics (AREA)
- Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
- General Health & Medical Sciences (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Investigating, Analyzing Materials By Fluorescence Or Luminescence (AREA)
- Optical Measuring Cells (AREA)
- Investigating Or Analysing Materials By Optical Means (AREA)
- Automatic Focus Adjustment (AREA)
- Length Measuring Devices By Optical Means (AREA)
- Investigating Or Analysing Biological Materials (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US245782 | 1999-02-05 | ||
| US09/245,782 US6130745A (en) | 1999-01-07 | 1999-02-05 | Optical autofocus for use with microtiter plates |
| PCT/US1999/009699 WO2000046590A1 (en) | 1999-02-05 | 1999-05-03 | Optical autofocus for use with microtiter plates |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE69938512D1 DE69938512D1 (de) | 2008-05-21 |
| DE69938512T2 true DE69938512T2 (de) | 2009-04-30 |
Family
ID=22928053
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69938512T Expired - Lifetime DE69938512T2 (de) | 1999-02-05 | 1999-05-03 | Optischer autofokus zur verwendung bei mikrotiterplatten |
| DE69941236T Expired - Lifetime DE69941236D1 (de) | 1999-02-05 | 1999-05-03 | Optischer Autofokus zur Verwendung mit Mikrotiter-Platten |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DE69941236T Expired - Lifetime DE69941236D1 (de) | 1999-02-05 | 1999-05-03 | Optischer Autofokus zur Verwendung mit Mikrotiter-Platten |
Country Status (5)
| Country | Link |
|---|---|
| US (2) | US6130745A (https=) |
| EP (2) | EP1942333B1 (https=) |
| JP (1) | JP4521121B2 (https=) |
| DE (2) | DE69938512T2 (https=) |
| WO (1) | WO2000046590A1 (https=) |
Families Citing this family (72)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20030030797A1 (en) * | 1999-09-24 | 2003-02-13 | Henry Palladino | Solid state fluorescence and absorption spectroscopy |
| US6586257B1 (en) * | 1999-10-12 | 2003-07-01 | Vertex Pharmaceuticals Incorporated | Multiwell scanner and scanning method |
| US6814933B2 (en) | 2000-09-19 | 2004-11-09 | Aurora Biosciences Corporation | Multiwell scanner and scanning method |
| FR2801977B1 (fr) * | 1999-12-02 | 2002-05-17 | Commissariat Energie Atomique | Amplification d'un signal de fluorescence emis par un echantillon surfacique |
| WO2002063367A1 (en) * | 2001-02-02 | 2002-08-15 | Cellomics, Inc. | Method for estimating the best initial focus position |
| FR2820828B1 (fr) * | 2001-02-09 | 2003-05-02 | Commissariat Energie Atomique | Dispositif d'observation d'echantillons par fluorescence, notamment de facon sequentielle |
| US6979530B2 (en) * | 2001-05-21 | 2005-12-27 | Applera Corporation | Peptide conjugates and fluorescence detection methods for intracellular caspase assay |
| DE10141544A1 (de) * | 2001-08-24 | 2003-03-13 | Eppendorf Ag | Vorrichtung zur Behandlung von Flüssigkeiten und Verfahren zum Betreiben der Vorrichtung |
| US6750457B2 (en) * | 2001-08-29 | 2004-06-15 | Becton Dickinson And Company | System for high throughput analysis |
| DE10157511A1 (de) * | 2001-11-23 | 2003-06-12 | Evotec Ag | Verfahren und Vorrichtung zur Korrektur der Größe und/oder Form eines Messvolumens in einer chemischen und/oder biologischen Probe |
| JP4497923B2 (ja) * | 2001-12-05 | 2010-07-07 | ザ ジェイ. デビッド グラッドストーン インスティテューツ | ロボット顕微鏡検査システム |
| FR2833718A1 (fr) * | 2001-12-13 | 2003-06-20 | Commissariat Energie Atomique | Dispositif d'observation d'objets, notamment pour plaques de microtitration |
| US7205154B2 (en) * | 2002-01-31 | 2007-04-17 | Agilent Technologies, Inc. | Calibrating array scanners |
| US6770892B2 (en) * | 2002-02-28 | 2004-08-03 | Agilent Technologies, Inc. | Method and system for automated focus-distance determination for molecular array scanners |
| IL148664A0 (en) * | 2002-03-13 | 2002-09-12 | Yeda Res & Dev | Auto-focusing method and device |
| WO2003095986A1 (en) | 2002-05-14 | 2003-11-20 | Amersham Biosciences Niagara, Inc. | System and methods for rapid and automated screening of cells |
| US9157860B2 (en) * | 2002-05-16 | 2015-10-13 | Applied Biosystems, Llc | Achromatic lens array |
| US6982166B2 (en) * | 2002-05-16 | 2006-01-03 | Applera Corporation | Lens assembly for biological testing |
| JPWO2004027085A1 (ja) * | 2002-09-05 | 2006-01-19 | 日東電工株式会社 | 固体表面の微生物試験用粘着シートおよびキット |
| JPWO2004022774A1 (ja) * | 2002-09-05 | 2005-12-22 | 富士電機システムズ株式会社 | 微生物または細胞の検出方法 |
| DE10244618A1 (de) * | 2002-09-25 | 2004-04-08 | Olympus Biosystems Gmbh | Einrichtung und Verfahren zur optischen Objektuntersuchung oder/und Erfassung oder/und Untersuchung von Schichten |
| EP1585978A4 (en) * | 2002-12-30 | 2009-12-16 | Pelikan Technologies Inc | METHOD AND APPARATUS FOR USING OPTICAL TECHNIQUES FOR MEASURING ANALYTE RATES |
| US20040150217A1 (en) * | 2003-01-23 | 2004-08-05 | Heffelfinger David M. | Identifying indicia and focusing target |
| US7522762B2 (en) * | 2003-04-16 | 2009-04-21 | Inverness Medical-Biostar, Inc. | Detection, resolution, and identification of arrayed elements |
| US6853454B1 (en) | 2004-01-15 | 2005-02-08 | Alpha Innotech Corporation | Optical analysis systems |
| US20050157299A1 (en) * | 2004-01-15 | 2005-07-21 | Heffelfinger David M. | Optical analysis systems |
| US6995901B2 (en) * | 2004-01-15 | 2006-02-07 | Alpha Innotech Corporation | Optical analysis systems |
| EP1760455A4 (en) * | 2004-06-21 | 2016-12-21 | Olympus Corp | MEASURING DEVICE |
| FI20055043A0 (fi) * | 2005-01-31 | 2005-01-31 | Wallac Oy | Sovitelma mikrotiterkaivojen kuvausjärjestelmässä |
| JP4701781B2 (ja) * | 2005-03-28 | 2011-06-15 | カシオ計算機株式会社 | 生体高分子分析装置及び生体高分子分析方法 |
| US7718131B2 (en) | 2005-07-06 | 2010-05-18 | Genetix Limited | Methods and apparatus for imaging and processing of samples in biological sample containers |
| EP1930717B1 (en) * | 2005-09-29 | 2019-06-19 | Olympus Corporation | Focal position determining method and apparatus |
| FR2898363B1 (fr) * | 2006-03-10 | 2008-06-13 | Biofilm Control Sarl | Prodede de releves d'images, notamment pour l'etude du developpement d'un biofilm dans un milieu de culture |
| CN101438147B (zh) | 2006-05-31 | 2011-09-28 | 奥林巴斯株式会社 | 生物试样摄像方法及生物试样摄像装置 |
| JP2008267842A (ja) * | 2007-04-17 | 2008-11-06 | Yokogawa Electric Corp | 生物観察容器並びにこれを用いる生物顕微鏡及び生物観察装置 |
| JP5152622B2 (ja) * | 2007-05-23 | 2013-02-27 | 横河電機株式会社 | 創薬スクリーニング装置及びこの装置を用いた創薬スクリーニング方法 |
| US7646482B2 (en) * | 2007-05-31 | 2010-01-12 | Genetix Limited | Methods and apparatus for optical analysis of samples in biological sample containers |
| US7535556B2 (en) * | 2007-05-31 | 2009-05-19 | Genetix Limited | Methods and apparatus for optical analysis of samples in biological sample containers |
| EP2110696B1 (en) | 2008-04-15 | 2013-10-16 | Sensovation AG | Method and apparatus for autofocus |
| FR2931966A1 (fr) * | 2008-05-30 | 2009-12-04 | Novacyt | Procede d'analyse cellulaire d'un prelevement au moyen d'une plaque d'analyse virtuelle |
| DE202009003288U1 (de) | 2009-03-11 | 2009-06-25 | Sensovation Ag | Autofokuseinrichtung |
| WO2010103389A1 (de) | 2009-03-11 | 2010-09-16 | Sensovation Ag | Autofokusverfahren und autofokuseinrichtung |
| DE102009012293A1 (de) | 2009-03-11 | 2010-09-16 | Sensovation Ag | Autofokusverfahren und Autofokuseinrichtung |
| JP5167194B2 (ja) * | 2009-04-28 | 2013-03-21 | 株式会社日立エンジニアリング・アンド・サービス | 微生物検査装置 |
| US7952799B2 (en) * | 2009-06-19 | 2011-05-31 | Corning Incorporated | Extreme broadband compact optical system with multiple fields of view |
| US8304704B2 (en) * | 2009-07-27 | 2012-11-06 | Sensovation Ag | Method and apparatus for autofocus using a light source pattern and means for masking the light source pattern |
| US9360659B2 (en) * | 2011-08-10 | 2016-06-07 | Molecular Devices, Llc | Method for presenting and evaluation of images of micro-titer plate properties |
| JP2012159854A (ja) * | 2012-04-04 | 2012-08-23 | Olympus Corp | 倒立顕微鏡システム |
| EP2769204B1 (en) | 2012-05-02 | 2016-02-17 | Charles River Laboratories, Inc. | Cell capture system and use thereof |
| EP2780707B1 (en) | 2012-05-02 | 2016-02-24 | Charles River Laboratories, Inc. | Method of detecting viable cells in a cell sample |
| AU2013256174B2 (en) | 2012-05-02 | 2017-03-02 | Charles River Laboratories, Inc. | Viability staining method |
| US20140168402A1 (en) * | 2012-12-13 | 2014-06-19 | Vala Sciences, Inc. | Continuous-Scanning Image Acquisition in Automated Microscopy Using Reflective Autofocus |
| EP2932425B1 (en) | 2012-12-14 | 2025-01-29 | The J. David Gladstone Institutes, a testamentary trust established under the Will of J. David Gladstone | Automated robotic microscopy systems |
| US9279750B2 (en) * | 2013-03-15 | 2016-03-08 | Iris International, Inc. | Method and composition for staining and sample processing |
| JP6523245B2 (ja) | 2013-03-15 | 2019-05-29 | アイリス インターナショナル, インコーポレイテッド | 血液試料における粒子分析のためのシース液システム及び方法 |
| US9857361B2 (en) * | 2013-03-15 | 2018-01-02 | Iris International, Inc. | Flowcell, sheath fluid, and autofocus systems and methods for particle analysis in urine samples |
| DE102013103971A1 (de) | 2013-04-19 | 2014-11-06 | Sensovation Ag | Verfahren zum Erzeugen eines aus mehreren Teilbildern zusammengesetzten Gesamtbilds eines Objekts |
| FR3012131B1 (fr) * | 2013-10-18 | 2018-01-19 | Centre National De La Recherche Scientifique | Supports amplificateurs de contraste pour l'observation d'un echantillon, leur procedes de fabrication et leurs utilisations |
| US10007102B2 (en) | 2013-12-23 | 2018-06-26 | Sakura Finetek U.S.A., Inc. | Microscope with slide clamping assembly |
| JP6673198B2 (ja) * | 2014-05-30 | 2020-03-25 | 株式会社ニコン | 顕微鏡 |
| US10941435B2 (en) | 2016-06-23 | 2021-03-09 | Life Technologies Corporation | Methods and compositions for detecting or measuring caspases or apoptosis |
| EP3287829A1 (en) * | 2016-08-25 | 2018-02-28 | Deutsches Krebsforschungszentrum | Method of and microscope with installation for focus stabilization |
| US11280803B2 (en) | 2016-11-22 | 2022-03-22 | Sakura Finetek U.S.A., Inc. | Slide management system |
| ES2924275T3 (es) * | 2017-01-19 | 2022-10-05 | Otsuka Pharma Co Ltd | Método de detección de posición y dispositivo de detección de posición para un chip sensor en un sistema óptico de detección de muestras |
| JP6853728B2 (ja) * | 2017-04-28 | 2021-03-31 | 株式会社Screenホールディングス | 試料容器およびこれを用いる撮像方法 |
| JP7212028B2 (ja) | 2017-07-13 | 2023-01-24 | グライナー バイオ‐ワン ノース アメリカ,インコーポレイテッド | 撮像用の培養プレート |
| CN108519332B (zh) * | 2018-04-28 | 2019-12-03 | 南通市肺科医院(南通市第六人民医院) | 全自动微孔板系统及用于该系统的计算机可读介质 |
| WO2019244275A1 (ja) * | 2018-06-20 | 2019-12-26 | 株式会社日立ハイテクノロジーズ | 観察装置 |
| GB201816687D0 (en) * | 2018-10-12 | 2018-11-28 | Clinspec Diagnostics Ltd | Sample container |
| EP4251974A4 (en) * | 2020-11-25 | 2024-11-20 | Mabtech Ab | APPARATUS AND METHOD FOR FOCUSING A LASER SCANNING CYTOMETER USING THE REFLECTION OF THE LASER |
| EP4198604B1 (en) * | 2021-12-17 | 2025-10-29 | Thermo Electron Scientific Instruments LLC | Method and system for positioning a sample |
| WO2024101416A1 (ja) * | 2022-11-10 | 2024-05-16 | キヤノン株式会社 | 走査方法、走査システム及び位置情報取得方法 |
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|---|---|---|---|---|
| FI790692A7 (fi) * | 1979-03-01 | 1980-09-02 | Suovaniemi Finnpipette | Mikrokyvettenhet |
| JPS5827755U (ja) * | 1981-08-19 | 1983-02-22 | オリンパス光学工業株式会社 | マイクロプレ−ト |
| JPS5891164U (ja) * | 1981-12-15 | 1983-06-20 | オリンパス光学工業株式会社 | 粒子凝集反応容器 |
| AU585033B2 (en) * | 1986-07-04 | 1989-06-08 | Tosoh Corporation | Quantitative dispenser for a liquid |
| JPS63193350U (https=) * | 1987-05-30 | 1988-12-13 | ||
| US4844617A (en) * | 1988-01-20 | 1989-07-04 | Tencor Instruments | Confocal measuring microscope with automatic focusing |
| JP2717730B2 (ja) * | 1991-01-09 | 1998-02-25 | 富士写真フイルム株式会社 | 液面レベル検出装置 |
| US5355215A (en) * | 1992-09-30 | 1994-10-11 | Environmental Research Institute Of Michigan | Method and apparatus for quantitative fluorescence measurements |
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-
1999
- 1999-02-05 US US09/245,782 patent/US6130745A/en not_active Expired - Lifetime
- 1999-05-03 EP EP08004221A patent/EP1942333B1/en not_active Expired - Lifetime
- 1999-05-03 JP JP2000597622A patent/JP4521121B2/ja not_active Expired - Fee Related
- 1999-05-03 EP EP99922783A patent/EP1169632B1/en not_active Expired - Lifetime
- 1999-05-03 WO PCT/US1999/009699 patent/WO2000046590A1/en not_active Ceased
- 1999-05-03 DE DE69938512T patent/DE69938512T2/de not_active Expired - Lifetime
- 1999-05-03 DE DE69941236T patent/DE69941236D1/de not_active Expired - Lifetime
- 1999-10-29 US US09/430,418 patent/US6441894B1/en not_active Expired - Lifetime
Also Published As
| Publication number | Publication date |
|---|---|
| US6130745A (en) | 2000-10-10 |
| JP4521121B2 (ja) | 2010-08-11 |
| EP1942333A1 (en) | 2008-07-09 |
| EP1169632A4 (en) | 2007-07-18 |
| DE69941236D1 (de) | 2009-09-17 |
| EP1942333B1 (en) | 2009-08-05 |
| DE69938512D1 (de) | 2008-05-21 |
| EP1169632B1 (en) | 2008-04-09 |
| EP1169632A1 (en) | 2002-01-09 |
| WO2000046590A1 (en) | 2000-08-10 |
| US6441894B1 (en) | 2002-08-27 |
| JP2002541430A (ja) | 2002-12-03 |
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