DE69834533T2 - Verfahren zur Protein-Herstellung - Google Patents

Verfahren zur Protein-Herstellung Download PDF

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Publication number
DE69834533T2
DE69834533T2 DE69834533T DE69834533T DE69834533T2 DE 69834533 T2 DE69834533 T2 DE 69834533T2 DE 69834533 T DE69834533 T DE 69834533T DE 69834533 T DE69834533 T DE 69834533T DE 69834533 T2 DE69834533 T2 DE 69834533T2
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DE
Germany
Prior art keywords
glucose
carbon source
promoter
glycerol
repressive
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69834533T
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German (de)
English (en)
Other versions
DE69834533D1 (de
Inventor
Karsten Hellmuth
Rual Lopez-Ulibarri
Anne Francoise New York Mayer
Heinrich Winfried Schlieker
Adolphus Van Loon
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
DSM IP Assets BV
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DSM IP Assets BV
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by DSM IP Assets BV filed Critical DSM IP Assets BV
Publication of DE69834533D1 publication Critical patent/DE69834533D1/de
Application granted granted Critical
Publication of DE69834533T2 publication Critical patent/DE69834533T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
DE69834533T 1997-10-01 1998-09-25 Verfahren zur Protein-Herstellung Expired - Lifetime DE69834533T2 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97117021 1997-10-01
EP97117021 1997-10-01

Publications (2)

Publication Number Publication Date
DE69834533D1 DE69834533D1 (de) 2006-06-22
DE69834533T2 true DE69834533T2 (de) 2006-10-12

Family

ID=8227420

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69834533T Expired - Lifetime DE69834533T2 (de) 1997-10-01 1998-09-25 Verfahren zur Protein-Herstellung

Country Status (12)

Country Link
US (1) US6204012B1 (https=)
EP (2) EP1659173A1 (https=)
JP (1) JP4162774B2 (https=)
KR (1) KR100572426B1 (https=)
CN (1) CN1215175C (https=)
AU (1) AU748187B2 (https=)
BR (1) BR9803807B1 (https=)
CA (1) CA2249061C (https=)
DE (1) DE69834533T2 (https=)
DK (1) DK0911416T3 (https=)
ES (1) ES2264181T3 (https=)
IN (1) IN188151B (https=)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
MXPA01009809A (es) * 1999-03-31 2002-07-30 Cornell Res Foundation Inc Fosfatasas con actividad de fitasa mejorada.
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
ES2597503T3 (es) 2001-10-31 2017-01-19 Huvepharma Eood Alimento para animales que contiene fitasa y método
KR100429331B1 (ko) * 2002-01-29 2004-04-29 재단법인 목암생명공학연구소 재조합 효모를 이용한 에스에이치엘케이엔-1 단백질의대량생산방법
CA2492267C (en) * 2002-07-15 2013-09-10 Immunex Corporation Methods and media for controlling sialylation of proteins produced by mammalian cells
US7309505B2 (en) * 2002-09-13 2007-12-18 Cornell Research Foundation, Inc. Using mutations to improve Aspergillus phytases
CA2534352A1 (en) * 2003-08-08 2005-02-17 Arriva Pharmaceuticals, Inc. Methods of protein production in yeast
JP2007511539A (ja) * 2003-11-14 2007-05-10 バクスター・インターナショナル・インコーポレイテッド α１−抗トリプシン組成物およびこのような組成物を用いた処置方法
EP2009093A3 (en) * 2004-09-22 2009-04-08 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
US20070105768A1 (en) * 2004-11-10 2007-05-10 Rajiv Nayar Dry recombinant human alpha 1-antitrypsin formulation
EP1861505A1 (en) * 2005-03-24 2007-12-05 DSMIP Assets B.V. Process for microbial production of a valuable compound
US7919297B2 (en) * 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
JP2009535038A (ja) * 2006-04-28 2009-10-01 シー５−６ テクノロジーズ インコーポレイテッド 耐熱性セルラーゼおよび使用方法
WO2008017066A2 (en) * 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
EP2106447B1 (en) 2007-01-26 2013-04-17 Novozymes A/S Method for methanol independent induction from methanol inducible promoters in pichia
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
HUE050997T2 (hu) * 2008-05-06 2021-03-01 Archer Daniels Midland Co Keményítõ- és xilánbontó enzimek expresszálásával keményítõ és xilán alkoholos fermentációjára képes hõtûrõ Hansenula polymorpha élesztõtörzsek kifejlesztése
GB201308828D0 (en) * 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
CN103159531A (zh) * 2013-04-17 2013-06-19 广西新方向化学工业有限公司 一种含多种植物醇和中微量元素螯合的液体肥料及其制备方法
JP2016525354A (ja) 2013-07-25 2016-08-25 ビーエーエスエフ エンザイムズ エルエルシー フィターゼ
JP2018501814A (ja) 2014-11-11 2018-01-25 クララ フーズ カンパニー 卵白タンパク質産生のための方法および組成物
CN104928271A (zh) * 2015-07-06 2015-09-23 唐亚军 一种木聚糖酶的制备方法
CN105200101B (zh) * 2015-10-19 2019-04-16 华中农业大学 一种毕赤酵母菌体重复发酵表达外源蛋白的方法
BR112022000545A2 (pt) 2019-07-11 2022-04-05 Clara Foods Co Composições de proteína e produtos de consumo destas
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
WO2025077804A1 (en) * 2023-10-12 2025-04-17 Chengdu Kanghua Biological Products Co., Ltd. Methanol monitoring method for the fermentation process of methanotrophic yeast

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0423890A3 (en) 1984-07-27 1991-07-03 Unilever Nv Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
NL8601454A (nl) * 1986-06-05 1988-01-04 Unilever Nv Werkwijze voor het bereiden van een catalase-vrij oxidoreductase en van een catalase-vrije oxidoreductase bevattende gist, en gebruik daarvan.
EP0242007B1 (en) * 1986-11-24 1990-11-07 Unilever N.V. Process for preparing a catalase-free oxidase and a catalase-free oxidase-containing yeast, and use thereof
ES2056799T3 (es) 1987-07-17 1994-10-16 Rhein Biotech Ges Fur Biotechn Moleculas de adn codante para las regiones de control fmdh y gen estructural para una proteina que tiene una actividad de fmdh y su uso.
JPH04501662A (ja) * 1988-09-26 1992-03-26 ザ・サルク・インスティチュート・バイオテクノロジー／インダストリアル・アソシエイツ・インコーポレーテッド 混合給送組換え酵母発酵
US5667986A (en) * 1989-10-18 1997-09-16 Delta Biotechnology Limited Yeast promoter for expressing heterologous polypeptides
FI932248L (fi) * 1991-09-18 1993-07-02 Amgen Inc Hepatit b-vaccinkomposition innehaollande ett gallsyrasalt
ES2268687T3 (es) 1994-04-25 2007-03-16 Dsm Ip Assets B.V. Polipeptidos con actividad fitasa.
US6068995A (en) * 1994-10-25 2000-05-30 Yoshitomi Pharmaceutical Industries, Ltd. Method for producing protein
KR0161148B1 (ko) * 1995-10-11 1998-11-16 김은영 재조합 메탄올 자화 효모를 사용한 이종 단백질의 생산방법
CA2231948C (en) 1997-03-25 2010-05-18 F. Hoffmann-La Roche Ag Modified phytases

Also Published As

Publication number Publication date
DE69834533D1 (de) 2006-06-22
CN1218112A (zh) 1999-06-02
CA2249061C (en) 2009-02-24
ES2264181T3 (es) 2006-12-16
KR100572426B1 (ko) 2006-06-21
EP0911416B1 (en) 2006-05-17
IN188151B (https=) 2002-08-24
BR9803807B1 (pt) 2010-11-03
BR9803807A (pt) 2000-03-28
US6204012B1 (en) 2001-03-20
AU8715798A (en) 1999-04-22
EP0911416A2 (en) 1999-04-28
CN1215175C (zh) 2005-08-17
KR19990036734A (ko) 1999-05-25
JPH11169173A (ja) 1999-06-29
EP1659173A1 (en) 2006-05-24
AU748187B2 (en) 2002-05-30
DK0911416T3 (da) 2006-08-14
EP0911416A3 (en) 2000-01-19
JP4162774B2 (ja) 2008-10-08
CA2249061A1 (en) 1999-04-01

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