KR100572426B1 - 단백질 제조방법 - Google Patents

단백질 제조방법 Download PDF

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Publication number
KR100572426B1
KR100572426B1 KR1019980041127A KR19980041127A KR100572426B1 KR 100572426 B1 KR100572426 B1 KR 100572426B1 KR 1019980041127 A KR1019980041127 A KR 1019980041127A KR 19980041127 A KR19980041127 A KR 19980041127A KR 100572426 B1 KR100572426 B1 KR 100572426B1
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South Korea
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carbon source
sugar
glucose
feed
promoter
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KR1019980041127A
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Korean (ko)
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KR19990036734A (ko
Inventor
카르스텐 헬무쓰
울리바리 루알 로페즈
안네 프랑소와즈 마이어
하인리히 빈프리트 쉴리커
룬 아돌푸스 반
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디에스엠 아이피 어셋츠 비.브이.
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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
KR1019980041127A 1997-10-01 1998-09-30 단백질 제조방법 Expired - Lifetime KR100572426B1 (ko)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP97117021 1997-10-01
EP97117021.2 1997-10-01

Publications (2)

Publication Number Publication Date
KR19990036734A KR19990036734A (ko) 1999-05-25
KR100572426B1 true KR100572426B1 (ko) 2006-06-21

Family

ID=8227420

Family Applications (1)

Application Number Title Priority Date Filing Date
KR1019980041127A Expired - Lifetime KR100572426B1 (ko) 1997-10-01 1998-09-30 단백질 제조방법

Country Status (12)

Country Link
US (1) US6204012B1 (https=)
EP (2) EP1659173A1 (https=)
JP (1) JP4162774B2 (https=)
KR (1) KR100572426B1 (https=)
CN (1) CN1215175C (https=)
AU (1) AU748187B2 (https=)
BR (1) BR9803807B1 (https=)
CA (1) CA2249061C (https=)
DE (1) DE69834533T2 (https=)
DK (1) DK0911416T3 (https=)
ES (1) ES2264181T3 (https=)
IN (1) IN188151B (https=)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6451572B1 (en) 1998-06-25 2002-09-17 Cornell Research Foundation, Inc. Overexpression of phytase genes in yeast systems
MXPA01009809A (es) * 1999-03-31 2002-07-30 Cornell Res Foundation Inc Fosfatasas con actividad de fitasa mejorada.
US6841370B1 (en) 1999-11-18 2005-01-11 Cornell Research Foundation, Inc. Site-directed mutagenesis of Escherichia coli phytase
ES2597503T3 (es) 2001-10-31 2017-01-19 Huvepharma Eood Alimento para animales que contiene fitasa y método
KR100429331B1 (ko) * 2002-01-29 2004-04-29 재단법인 목암생명공학연구소 재조합 효모를 이용한 에스에이치엘케이엔-1 단백질의대량생산방법
CA2492267C (en) * 2002-07-15 2013-09-10 Immunex Corporation Methods and media for controlling sialylation of proteins produced by mammalian cells
US7309505B2 (en) * 2002-09-13 2007-12-18 Cornell Research Foundation, Inc. Using mutations to improve Aspergillus phytases
CA2534352A1 (en) * 2003-08-08 2005-02-17 Arriva Pharmaceuticals, Inc. Methods of protein production in yeast
JP2007511539A (ja) * 2003-11-14 2007-05-10 バクスター・インターナショナル・インコーポレイテッド α１−抗トリプシン組成物およびこのような組成物を用いた処置方法
EP2009093A3 (en) * 2004-09-22 2009-04-08 Bioworks, Inc. Transgenic strains of trichoderma and their use in biocontrol
US20070105768A1 (en) * 2004-11-10 2007-05-10 Rajiv Nayar Dry recombinant human alpha 1-antitrypsin formulation
EP1861505A1 (en) * 2005-03-24 2007-12-05 DSMIP Assets B.V. Process for microbial production of a valuable compound
US7919297B2 (en) * 2006-02-21 2011-04-05 Cornell Research Foundation, Inc. Mutants of Aspergillus niger PhyA phytase and Aspergillus fumigatus phytase
JP2009535038A (ja) * 2006-04-28 2009-10-01 シー５−６ テクノロジーズ インコーポレイテッド 耐熱性セルラーゼおよび使用方法
WO2008017066A2 (en) * 2006-08-03 2008-02-07 Cornell Research Foundation, Inc. Phytases with improved thermal stability
EP2106447B1 (en) 2007-01-26 2013-04-17 Novozymes A/S Method for methanol independent induction from methanol inducible promoters in pichia
US8192734B2 (en) 2007-07-09 2012-06-05 Cornell University Compositions and methods for bone strengthening
HUE050997T2 (hu) * 2008-05-06 2021-03-01 Archer Daniels Midland Co Keményítõ- és xilánbontó enzimek expresszálásával keményítõ és xilán alkoholos fermentációjára képes hõtûrõ Hansenula polymorpha élesztõtörzsek kifejlesztése
GB201308828D0 (en) * 2013-03-12 2013-07-03 Verenium Corp Phytase
GB201308843D0 (en) 2013-03-14 2013-07-03 Verenium Corp Phytase formulation
CN103159531A (zh) * 2013-04-17 2013-06-19 广西新方向化学工业有限公司 一种含多种植物醇和中微量元素螯合的液体肥料及其制备方法
JP2016525354A (ja) 2013-07-25 2016-08-25 ビーエーエスエフ エンザイムズ エルエルシー フィターゼ
JP2018501814A (ja) 2014-11-11 2018-01-25 クララ フーズ カンパニー 卵白タンパク質産生のための方法および組成物
CN104928271A (zh) * 2015-07-06 2015-09-23 唐亚军 一种木聚糖酶的制备方法
CN105200101B (zh) * 2015-10-19 2019-04-16 华中农业大学 一种毕赤酵母菌体重复发酵表达外源蛋白的方法
BR112022000545A2 (pt) 2019-07-11 2022-04-05 Clara Foods Co Composições de proteína e produtos de consumo destas
US12096784B2 (en) 2019-07-11 2024-09-24 Clara Foods Co. Protein compositions and consumable products thereof
US10927360B1 (en) 2019-08-07 2021-02-23 Clara Foods Co. Compositions comprising digestive enzymes
WO2025077804A1 (en) * 2023-10-12 2025-04-17 Chengdu Kanghua Biological Products Co., Ltd. Methanol monitoring method for the fermentation process of methanotrophic yeast

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0244920A1 (en) * 1986-06-05 1987-11-11 Unilever N.V. Process for preparing a catalase-free oxidase and a catalase-free oxidase-containing yeast, and use thereof
WO1990003431A1 (en) * 1988-09-26 1990-04-05 The Salk Institute Biotechnology/Industrial Associates, Inc. Mixed feed recombinant yeast fermentation
EP0533492A2 (en) * 1991-09-18 1993-03-24 Amgen Inc. A hepatitis B vaccine formulation incorporating a bile acid salt
KR970700775A (ko) * 1994-10-25 1997-02-12 가와노 다케히코 단백질 제조방법(Method for Producing Protein)
KR0161148B1 (ko) * 1995-10-11 1998-11-16 김은영 재조합 메탄올 자화 효모를 사용한 이종 단백질의 생산방법

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0423890A3 (en) 1984-07-27 1991-07-03 Unilever Nv Use of oxidoreductases in bleaching and/or detergent compositions and their preparation by microorganisms engineered by recombinant dna technology
US4855231A (en) 1984-10-30 1989-08-08 Phillips Petroleum Company Regulatory region for heterologous gene expression in yeast
EP0242007B1 (en) * 1986-11-24 1990-11-07 Unilever N.V. Process for preparing a catalase-free oxidase and a catalase-free oxidase-containing yeast, and use thereof
ES2056799T3 (es) 1987-07-17 1994-10-16 Rhein Biotech Ges Fur Biotechn Moleculas de adn codante para las regiones de control fmdh y gen estructural para una proteina que tiene una actividad de fmdh y su uso.
US5667986A (en) * 1989-10-18 1997-09-16 Delta Biotechnology Limited Yeast promoter for expressing heterologous polypeptides
ES2268687T3 (es) 1994-04-25 2007-03-16 Dsm Ip Assets B.V. Polipeptidos con actividad fitasa.
CA2231948C (en) 1997-03-25 2010-05-18 F. Hoffmann-La Roche Ag Modified phytases

Patent Citations (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0244920A1 (en) * 1986-06-05 1987-11-11 Unilever N.V. Process for preparing a catalase-free oxidase and a catalase-free oxidase-containing yeast, and use thereof
WO1990003431A1 (en) * 1988-09-26 1990-04-05 The Salk Institute Biotechnology/Industrial Associates, Inc. Mixed feed recombinant yeast fermentation
EP0533492A2 (en) * 1991-09-18 1993-03-24 Amgen Inc. A hepatitis B vaccine formulation incorporating a bile acid salt
KR970700775A (ko) * 1994-10-25 1997-02-12 가와노 다케히코 단백질 제조방법(Method for Producing Protein)
KR100405944B1 (ko) * 1994-10-25 2004-03-22 미쯔비시 웰 파마 가부시키가이샤 단백질 제조방법
KR0161148B1 (ko) * 1995-10-11 1998-11-16 김은영 재조합 메탄올 자화 효모를 사용한 이종 단백질의 생산방법

Also Published As

Publication number Publication date
DE69834533D1 (de) 2006-06-22
CN1218112A (zh) 1999-06-02
CA2249061C (en) 2009-02-24
ES2264181T3 (es) 2006-12-16
EP0911416B1 (en) 2006-05-17
IN188151B (https=) 2002-08-24
BR9803807B1 (pt) 2010-11-03
BR9803807A (pt) 2000-03-28
US6204012B1 (en) 2001-03-20
AU8715798A (en) 1999-04-22
EP0911416A2 (en) 1999-04-28
CN1215175C (zh) 2005-08-17
KR19990036734A (ko) 1999-05-25
JPH11169173A (ja) 1999-06-29
DE69834533T2 (de) 2006-10-12
EP1659173A1 (en) 2006-05-24
AU748187B2 (en) 2002-05-30
DK0911416T3 (da) 2006-08-14
EP0911416A3 (en) 2000-01-19
JP4162774B2 (ja) 2008-10-08
CA2249061A1 (en) 1999-04-01

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