DE69719557T2 - Verbesserte erkennung von spezifischen nukleinsäuren - Google Patents

Verbesserte erkennung von spezifischen nukleinsäuren Download PDF

Info

Publication number
DE69719557T2
DE69719557T2 DE69719557T DE69719557T DE69719557T2 DE 69719557 T2 DE69719557 T2 DE 69719557T2 DE 69719557 T DE69719557 T DE 69719557T DE 69719557 T DE69719557 T DE 69719557T DE 69719557 T2 DE69719557 T2 DE 69719557T2
Authority
DE
Germany
Prior art keywords
probe
target
function
sequence
nucleic acid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69719557T
Other languages
German (de)
English (en)
Other versions
DE69719557D1 (de
Inventor
Ulf Landegren
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Landegren Gene Technology AB
Original Assignee
Landegren Gene Technology AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Landegren Gene Technology AB filed Critical Landegren Gene Technology AB
Publication of DE69719557D1 publication Critical patent/DE69719557D1/de
Application granted granted Critical
Publication of DE69719557T2 publication Critical patent/DE69719557T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
DE69719557T 1996-04-30 1997-04-30 Verbesserte erkennung von spezifischen nukleinsäuren Expired - Lifetime DE69719557T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9601676A SE9601676D0 (sv) 1996-04-30 1996-04-30 Improved probing of specific mucleic acids
SE9601676 1996-04-30
PCT/SE1997/000737 WO1997041254A1 (en) 1996-04-30 1997-04-30 Improved probing of specific nucleic acids

Publications (2)

Publication Number Publication Date
DE69719557D1 DE69719557D1 (de) 2003-04-10
DE69719557T2 true DE69719557T2 (de) 2004-02-26

Family

ID=20402429

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69719557T Expired - Lifetime DE69719557T2 (de) 1996-04-30 1997-04-30 Verbesserte erkennung von spezifischen nukleinsäuren

Country Status (8)

Country Link
US (2) US20010007742A1 (enExample)
EP (1) EP0951565B1 (enExample)
JP (1) JP2000508920A (enExample)
AT (1) ATE233826T1 (enExample)
CA (1) CA2252817C (enExample)
DE (1) DE69719557T2 (enExample)
SE (1) SE9601676D0 (enExample)
WO (1) WO1997041254A1 (enExample)

Families Citing this family (80)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO2004046344A2 (en) * 2002-11-19 2004-06-03 Applera Corporation Polynucleotide sequence detection assays
EP1572974A2 (en) * 2002-11-19 2005-09-14 Applera Corporation Polynucleotide sequence detection assays and analysis
US7799525B2 (en) 2003-06-17 2010-09-21 Human Genetic Signatures Pty Ltd. Methods for genome amplification
EP1668148B1 (en) * 2003-09-04 2008-12-31 Human Genetic Signatures PTY Ltd. Nucleic acid detection assay
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
WO2005113804A1 (en) * 2004-05-20 2005-12-01 Trillion Genomics Limited Use of mass labelled probes to detect target nucleic acids using mass spectrometry
DE602005022740D1 (de) 2004-09-10 2010-09-16 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)
BRPI0517131B1 (pt) 2004-12-03 2021-08-31 Human Genetic Signatures Pty Ltd Métodos para simplificar ácidos nucléicos microbiais por modificação química de citosinas
WO2006125267A1 (en) 2005-05-26 2006-11-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
WO2007030882A1 (en) 2005-09-14 2007-03-22 Human Genetic Signatures Pty Ltd Assay for a health state
EP1879377A3 (en) * 2006-07-13 2010-06-16 Panasonic Corporation Portable Device
WO2009067743A1 (en) 2007-11-27 2009-06-04 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
WO2009121021A1 (en) * 2008-03-28 2009-10-01 Life Technologies Corporation Degradable arrays for preventing reuse
ES2637411T3 (es) 2008-12-01 2017-10-13 Laboratory Corporation Of America Holdings Métodos y ensayos para medir p95 Y/O p95 en una muestra y anticuerpos específicos para p95
SG172983A1 (en) 2009-01-15 2011-08-29 Lab Corp America Holdings Methods of determining patient response by measurement of her-3
CA2749817A1 (en) * 2009-01-15 2010-07-22 Laboratory Corporation Of America Holdings Methods of determining patient response by measurement of her-2 expression
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
CA2760439A1 (en) 2009-04-30 2010-11-04 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
JP2013511991A (ja) * 2009-11-25 2013-04-11 クアンタライフ， インコーポレイテッド 遺伝子材料を検出する方法および組成物
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
EP3483285B1 (en) 2011-02-09 2021-07-14 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
US9556473B2 (en) 2011-02-15 2017-01-31 Leica Biosystems Newcastle Ltd Methods for identifying nucleic acid sequences
CA2827497C (en) 2011-02-15 2014-12-02 Leica Biosystems Newcastle Ltd. Method for localized in situ detection of mrna
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
US8932819B2 (en) 2011-07-06 2015-01-13 Affymetrix, Inc. Assays for affinity profiling of nucleic acid binding proteins
BR112014005253B1 (pt) 2011-09-07 2019-11-05 Human Genetic Signatures Pty Ltd método de detecção molecular
CA2852665A1 (en) 2011-10-17 2013-04-25 Good Start Genetics, Inc. Analysis methods
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
KR20230169418A (ko) 2013-03-13 2023-12-15 메소 스케일 테크놀러지즈, 엘엘시 개선된 분석 방법
WO2014152421A1 (en) 2013-03-14 2014-09-25 Good Start Genetics, Inc. Methods for analyzing nucleic acids
EP2970631B1 (en) 2013-03-15 2017-05-03 Lubrizol Advanced Materials, Inc. Heavy metal free cpvc compositions
US8847799B1 (en) 2013-06-03 2014-09-30 Good Start Genetics, Inc. Methods and systems for storing sequence read data
US9944998B2 (en) 2013-07-25 2018-04-17 Bio-Rad Laboratories, Inc. Genetic assays
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US11053548B2 (en) 2014-05-12 2021-07-06 Good Start Genetics, Inc. Methods for detecting aneuploidy
CN119709943A (zh) 2014-05-15 2025-03-28 中尺度技术有限责任公司 改进的测定方法
JP6976167B2 (ja) 2014-06-13 2021-12-08 キュー−リネア エービー 微生物の検出及び特徴付け方法
US20160048608A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
WO2016040446A1 (en) 2014-09-10 2016-03-17 Good Start Genetics, Inc. Methods for selectively suppressing non-target sequences
EP3224595A4 (en) 2014-09-24 2018-06-13 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
EP3209798B1 (en) 2014-10-23 2022-07-27 Ricardo Mancebo Reagents and methods for isothermal chain reaction
US10066259B2 (en) 2015-01-06 2018-09-04 Good Start Genetics, Inc. Screening for structural variants
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
EP3608420B1 (en) 2015-07-29 2021-05-19 Progenity, Inc. Nucleic acids and methods for detecting chromosomal abnormalities
CN108779487A (zh) 2015-11-16 2018-11-09 普罗格尼迪公司 用于检测甲基化状态的核酸和方法
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
WO2018102759A1 (en) 2016-12-01 2018-06-07 Ignite Biosciences, Inc. Methods of assaying proteins
WO2018183607A1 (en) 2017-03-30 2018-10-04 Pioneer Hi-Bred International, Inc. Methods of identifying and characterizing gene editing variations in nucleic acids
KR20250152680A (ko) 2017-12-29 2025-10-23 노틸러스 서브시디어리, 인크. 단백질 식별을 위한 디코딩 접근법
CN120905352A (zh) 2018-04-02 2025-11-07 埃努梅拉分子股份有限公司 用于对核酸分子进行计数的方法、系统和组合物
EP3931201A1 (en) 2019-03-01 2022-01-05 Meso Scale Technologies, LLC Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same
EP3947718A4 (en) 2019-04-02 2022-12-21 Enumera Molecular, Inc. METHODS, SYSTEMS AND COMPOSITIONS FOR COUNTING NUCLEIC ACID MOLECULES
CN115066434A (zh) 2019-12-03 2022-09-16 阿拉玛生物科学公司 核酸连接的免疫-夹心测定(nulisa)
WO2021252800A1 (en) 2020-06-11 2021-12-16 Nautilus Biotechnology, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
AU2021345359A1 (en) 2020-09-21 2023-05-11 Enumera Molecular, Inc. Compositions and methods for isolation of cell-free dna
CA3203106A1 (en) 2021-01-20 2022-07-28 Parag Mallick Systems and methods for biomolecule quantitation
EP4281775A1 (en) 2021-01-21 2023-11-29 Nautilus Subsidiary, Inc. Systems and methods for biomolecule preparation
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
WO2023049073A1 (en) 2021-09-22 2023-03-30 Nautilus Biotechnology, Inc. Methods and systems for determining polypeptide interactions
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
EP4500157A1 (en) 2022-03-29 2025-02-05 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
WO2023212490A1 (en) 2022-04-25 2023-11-02 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
EP4544077A1 (en) 2022-06-21 2025-04-30 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
WO2024124073A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. A method comprising performing on a single-analyte array at least 50 cycles of a process
EP4634285A1 (en) 2022-12-15 2025-10-22 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
US20240344116A1 (en) 2023-03-24 2024-10-17 Nautilus Subsidiary, Inc. Transfer of nanoparticles to array surfaces
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
WO2025043159A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
WO2025137166A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
US20250305034A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
SE9400522D0 (sv) * 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5643724A (en) * 1994-06-06 1997-07-01 Roche Molecular Systems, Inc. Methods and reagents for Glycophorin A typing
GB9414934D0 (en) * 1994-07-25 1994-09-14 Raggio Italgene Spa Method and reagents for the detection of target nuceic acid sequences
NZ298494A (en) 1995-07-11 1999-09-29 Forfas Trading As Bioresearch Glycosylase mediated detection of nucleotide sequences at candidate loci

Also Published As

Publication number Publication date
CA2252817A1 (en) 1997-11-06
US20020102592A1 (en) 2002-08-01
WO1997041254A1 (en) 1997-11-06
SE9601676D0 (sv) 1996-04-30
US20010007742A1 (en) 2001-07-12
ATE233826T1 (de) 2003-03-15
US7351528B2 (en) 2008-04-01
EP0951565A1 (en) 1999-10-27
CA2252817C (en) 2009-07-07
DE69719557D1 (de) 2003-04-10
EP0951565B1 (en) 2003-03-05
JP2000508920A (ja) 2000-07-18

Similar Documents

Publication Publication Date Title
DE69719557T2 (de) Verbesserte erkennung von spezifischen nukleinsäuren
DE69421277T2 (de) NUKLEINSäURE-SEQUENZANALYSE DURCH DIE METHODE DER PARALLELEN PRIMEREXTENSION
DE69836885T2 (de) Nachweis von Nukleinsäuren durch Fluoreszenz quenching
DE69531542T2 (de) Ligase/polymerase-vermittelte analyse genetischer elemente von einzelnukleotid-polymorphismen und ihre verwendung in der genetischen analyse
DE69233719T2 (de) Primer, Sätze und Restriktionsfragmente und deren Benutzung in selektiver Restriktionsfragmentenamplifikation
DE69434066T2 (de) Verfahren zur immobilisierung von nukleinsäuremolekülen
DE60118655T2 (de) Sonden und Methoden zum Nachweis von Nukleinsäuren
DE69432919T2 (de) Verfahren für den Nachweis spezifischer Polynukleotiden
DE69118930T2 (de) Verbessertes Verfahren zur Amplifikation von Nuklein säurezielsequenz, einsetzbar für die Polymerase und Ligasekettenreaktion
DE69333650T2 (de) Neue anordnungn von oligonukleotiden und ihr nutzen zum sortieren, isolieren, sequenzieren und manipulieren von nukleinsäuren
DE68926302T2 (de) Nucleinsäuresonden, welche verbesserte molekularschalter enthalten, und analysemethoden und kits, bei welchen die sonden verwendet werden
DE69328699T2 (de) Hybridisierungsassay unter Verwendung von verzweigten Nukleinsäuresonden
DE69516116T2 (de) Sequenzierung von nukleinsäuren
DE68909266T2 (de) Markierung durch gleichzeitige Ligation und Restriktion.
DE69916877T2 (de) Ligations-vermittelte assemblierung und nachweis von polynukleotiden auf einem festträger
DE69706430T2 (de) Verfahren zur sequenzierung von dna
DE68915202T3 (de) Verfahren zur Vermehrung und zum Nachweis von Nukleinsäuresequenzen.
DE69128077T2 (de) Nuklein-säure amplifikation mit dns abhängigen rns polymerasen aktivität von rns-replikasen
DE69635612T2 (de) Detektion und amplifikation von nukleinsäuren durch chemische verbindung von oligonukleotiden
DE69527392T2 (de) Nachweis von Nukleinsäure-Amplifikation
DE60029876T2 (de) Verfahren und Oligonukleotide zum Nachweis von Nukleinsäuresequenzvariationen
DE69430665T2 (de) Empfindlicher nukleinsäure-sandwichhybridisierungs-assay und kits
DE69301570T2 (de) Verfahren für die sequenzierung von synthetischen oligonukleotiden, welche internukleotid-bindungen enthalten, die nicht vom phosphodiester-typ sind
DE69028725T2 (de) Partiell doppelsträngiges Oligonukleotid und Verfahren zu seiner Bildung
EP0705905B1 (de) Verfahren zum sensitiven Nachweis von Nukleinsäuren

Legal Events

Date Code Title Description
8327 Change in the person/name/address of the patent owner

Owner name: LANDEGREN GENE TECHNOLOGY AB, UPPSALA, SE

8381 Inventor (new situation)

Inventor name: LANDEGREN, ULF, 756 45 UPPSALA, SE

8364 No opposition during term of opposition