CA2252817C - Improved probing of specific nucleic acids - Google Patents

Improved probing of specific nucleic acids Download PDF

Info

Publication number
CA2252817C
CA2252817C CA002252817A CA2252817A CA2252817C CA 2252817 C CA2252817 C CA 2252817C CA 002252817 A CA002252817 A CA 002252817A CA 2252817 A CA2252817 A CA 2252817A CA 2252817 C CA2252817 C CA 2252817C
Authority
CA
Canada
Prior art keywords
probe
solid phase
nucleic acid
padlock
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002252817A
Other languages
English (en)
French (fr)
Other versions
CA2252817A1 (en
Inventor
Ulf Landegren
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olink Holding AB
Original Assignee
Olink AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olink AB filed Critical Olink AB
Publication of CA2252817A1 publication Critical patent/CA2252817A1/en
Application granted granted Critical
Publication of CA2252817C publication Critical patent/CA2252817C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CA002252817A 1996-04-30 1997-04-30 Improved probing of specific nucleic acids Expired - Lifetime CA2252817C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9601676A SE9601676D0 (sv) 1996-04-30 1996-04-30 Improved probing of specific mucleic acids
SE9601676-1 1996-04-30
PCT/SE1997/000737 WO1997041254A1 (en) 1996-04-30 1997-04-30 Improved probing of specific nucleic acids

Publications (2)

Publication Number Publication Date
CA2252817A1 CA2252817A1 (en) 1997-11-06
CA2252817C true CA2252817C (en) 2009-07-07

Family

ID=20402429

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002252817A Expired - Lifetime CA2252817C (en) 1996-04-30 1997-04-30 Improved probing of specific nucleic acids

Country Status (8)

Country Link
US (2) US20010007742A1 (enExample)
EP (1) EP0951565B1 (enExample)
JP (1) JP2000508920A (enExample)
AT (1) ATE233826T1 (enExample)
CA (1) CA2252817C (enExample)
DE (1) DE69719557T2 (enExample)
SE (1) SE9601676D0 (enExample)
WO (1) WO1997041254A1 (enExample)

Families Citing this family (86)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
WO1994003624A1 (en) * 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
WO2004046344A2 (en) * 2002-11-19 2004-06-03 Applera Corporation Polynucleotide sequence detection assays
EP1572974A2 (en) * 2002-11-19 2005-09-14 Applera Corporation Polynucleotide sequence detection assays and analysis
WO2004111266A1 (en) 2003-06-17 2004-12-23 Human Genetic Signatures Pty Ltd Methods for genome amplification
CA2537810C (en) * 2003-09-04 2012-12-18 Human Genetic Signatures Pty Ltd Nucleic acid detection assay
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
WO2005113804A1 (en) * 2004-05-20 2005-12-01 Trillion Genomics Limited Use of mass labelled probes to detect target nucleic acids using mass spectrometry
WO2006026828A1 (en) 2004-09-10 2006-03-16 Human Genetic Signatures Pty Ltd Amplification blocker comprising intercalating nucleic acids (tna) containing intercalating pseudonucleotides (ipn)
CN101111606B (zh) 2004-12-03 2012-05-16 人类遗传标记控股有限公司 通过化学修饰胞嘧啶而简化微生物核酸的方法
EP1883707B1 (en) 2005-05-26 2014-04-09 Human Genetic Signatures PTY Ltd Isothermal strand displacement amplification using primers containing a non-regular base
CA2621932A1 (en) 2005-09-14 2007-03-22 Human Genetic Signatures Pty Ltd Assay for hpv and methylated genomic dna targets to screen for cervical cancer
EP1879377A3 (en) * 2006-07-13 2010-06-16 Panasonic Corporation Portable Device
CA2706740C (en) 2007-11-27 2017-01-17 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
WO2009121021A1 (en) * 2008-03-28 2009-10-01 Life Technologies Corporation Degradable arrays for preventing reuse
US20110159499A1 (en) * 2009-11-25 2011-06-30 Quantalife, Inc. Methods and compositions for detecting genetic material
EP2438442B1 (en) 2008-12-01 2017-08-09 Laboratory Corporation of America Holdings Methods and assays for measuring p95 and/or p95 complexes in a sample and antibodies specific for p95
US20100233732A1 (en) * 2009-01-15 2010-09-16 Laboratory Corporation Of America Holdings Methods of Determining Patient Response By Measurement of HER-2 Expression
BRPI1007321A2 (en) 2009-01-15 2018-07-10 Laboratory Corporation Of America Holdings methods for measuring and / or quantifying the presence and / or amount of her-3 or her-3 in a complex in a patient sample, and to determine if an individual with cancer is likely to respond to treatment with a targeted therapy. and antibody.
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
EP2425240A4 (en) 2009-04-30 2012-12-12 Good Start Genetics Inc METHOD AND COMPOSITION FOR EVALUATING GENETIC MARKERS
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
EP3483285B1 (en) 2011-02-09 2021-07-14 Bio-Rad Laboratories, Inc. Analysis of nucleic acids
US9556473B2 (en) 2011-02-15 2017-01-31 Leica Biosystems Newcastle Ltd Methods for identifying nucleic acid sequences
JP5881746B2 (ja) 2011-02-15 2016-03-09 ライカ バイオシステムズ ニューキャッスル リミテッド ｍＲＮＡの局在インサイチュー検出のための方法
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
US8932819B2 (en) 2011-07-06 2015-01-13 Affymetrix, Inc. Assays for affinity profiling of nucleic acid binding proteins
KR101912488B1 (ko) 2011-09-07 2018-10-26 휴먼 제네틱 시그너처스 피티와이 엘티디 분자 검출 분석법
EP2768983A4 (en) 2011-10-17 2015-06-03 Good Start Genetics Inc METHODS OF IDENTIFYING MUTATIONS ASSOCIATED WITH DISEASES
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
CN117288934A (zh) 2013-03-13 2023-12-26 中尺度技术有限责任公司 改进的测定方法
EP2971159B1 (en) 2013-03-14 2019-05-08 Molecular Loop Biosolutions, LLC Methods for analyzing nucleic acids
CN105209532B (zh) 2013-03-15 2017-11-28 路博润先进材料公司 不含重金属的cpvc组合物
WO2014197377A2 (en) 2013-06-03 2014-12-11 Good Start Genetics, Inc. Methods and systems for storing sequence read data
CN105555972B (zh) 2013-07-25 2020-07-31 伯乐生命医学产品有限公司 遗传测定
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
US11041203B2 (en) 2013-10-18 2021-06-22 Molecular Loop Biosolutions, Inc. Methods for assessing a genomic region of a subject
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
KR102757668B1 (ko) 2014-05-15 2025-01-21 메소 스케일 테크놀러지즈, 엘엘시 개선된 분석 방법
WO2015189390A1 (en) 2014-06-13 2015-12-17 Q-Linea Ab Method for detecting and characterising a microorganism
WO2016025818A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
US10429399B2 (en) 2014-09-24 2019-10-01 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
EP4098753A1 (en) 2014-10-23 2022-12-07 Ricardo Mancebo Reagents and methods for isothermal chain reaction
EP3271480B8 (en) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Screening for structural variants
JP6929778B2 (ja) 2015-01-15 2021-09-01 グッド スタート ジェネティクス， インコーポレイテッド 着床前遺伝子スクリーニングにおける一塩基多型を用いた品質管理方法
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
CN108350500A (zh) 2015-07-29 2018-07-31 普罗格尼迪公司 用于检测染色体异常的核酸和方法
EP3377647B1 (en) 2015-11-16 2020-03-11 Progenity, Inc. Nucleic acids and methods for detecting methylation status
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
JP2020514746A (ja) 2016-12-01 2020-05-21 ノーティラス バイオテクノロジー インコーポレイテッド タンパク質をアッセイする方法
US11661624B2 (en) 2017-03-30 2023-05-30 Pioneer Hi-Bred International, Inc. Methods of identifying and characterizing gene editing variations in nucleic acids
CA3086915A1 (en) 2017-12-29 2019-07-04 Sujal M. Patel Decoding approaches for protein identification
EP3775272B1 (en) 2018-04-02 2025-07-30 Enumera Molecular, Inc. Methods, systems, and compositions for counting nucleic acid molecules
CN120098057A (zh) 2019-03-01 2025-06-06 中尺度技术有限责任公司 用于免疫方法的被电化学发光标记的探针、使用这类探针的方法和包含这类探针的试剂盒
WO2020206170A1 (en) 2019-04-02 2020-10-08 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
JP7727628B2 (ja) 2019-12-03 2025-08-21 アラマー バイオサイエンシーズ， インク． 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ）
GB202004469D0 (en) 2020-03-27 2020-05-13 Olink Proteomics Ab Controls for proximity detection assays
CA3182266A1 (en) 2020-06-11 2021-12-16 Vadim Lobanov Methods and systems for computational decoding of biological, chemical, and physical entities
WO2022061305A1 (en) 2020-09-21 2022-03-24 Progenity, Inc. Compositions and methods for isolation of cell-free dna
CA3203106A1 (en) 2021-01-20 2022-07-28 Parag Mallick Systems and methods for biomolecule quantitation
US20220227890A1 (en) 2021-01-21 2022-07-21 Nautilus Biotechnology, Inc. Systems and methods for biomolecule preparation
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
US12704508B2 (en) 2021-06-02 2026-08-11 Alamar Biosciences, Inc. Nucleic acid linked immune-sandwich assay (NULISA)
AU2022304570A1 (en) 2021-06-28 2024-01-18 Meso Scale Technologies, Llc. Methods, compositions, and kits for assay signal amplification
EP4399528A1 (en) 2021-09-09 2024-07-17 Nautilus Subsidiary, Inc. Characterization and localization of protein modifications
EP4405683A1 (en) 2021-09-22 2024-07-31 Nautilus Subsidiary, Inc. Methods and systems for determining polypeptide interactions
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
EP4441503A1 (en) 2021-11-30 2024-10-09 Nautilus Subsidiary, Inc. Particle-based isolation of proteins and other analytes
CA3245990A1 (en) 2022-03-29 2023-10-05 Nautilus Subsidiary, Inc. INTEGRATED NETWORKS FOR SINGLE-ANALYTE PROCESSES
EP4515026A1 (en) 2022-04-25 2025-03-05 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
WO2023250364A1 (en) 2022-06-21 2023-12-28 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
WO2024059655A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
US20240192202A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. Fluidic media for single-analtye arrays
EP4634285A1 (en) 2022-12-15 2025-10-22 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
EP4689173A1 (en) 2023-03-24 2026-02-11 Nautilus Subsidiary, Inc. Improved transfer of nanoparticles to array surfaces
US20240394902A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
US20250066841A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
US20250189519A1 (en) 2023-12-11 2025-06-12 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
US20250205706A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
US20250298016A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
US20250305034A1 (en) 2024-04-01 2025-10-02 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
SE9400522D0 (sv) * 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5643724A (en) * 1994-06-06 1997-07-01 Roche Molecular Systems, Inc. Methods and reagents for Glycophorin A typing
GB9414934D0 (en) * 1994-07-25 1994-09-14 Raggio Italgene Spa Method and reagents for the detection of target nuceic acid sequences
EP0843735B2 (en) 1995-07-11 2006-12-27 Forfas (trading as Bioresearch Ireland) Glycosylase mediated detection of known nucleotide sequences

Also Published As

Publication number Publication date
EP0951565A1 (en) 1999-10-27
JP2000508920A (ja) 2000-07-18
DE69719557T2 (de) 2004-02-26
CA2252817A1 (en) 1997-11-06
US20020102592A1 (en) 2002-08-01
US20010007742A1 (en) 2001-07-12
WO1997041254A1 (en) 1997-11-06
SE9601676D0 (sv) 1996-04-30
DE69719557D1 (de) 2003-04-10
EP0951565B1 (en) 2003-03-05
ATE233826T1 (de) 2003-03-15
US7351528B2 (en) 2008-04-01

Similar Documents

Publication Publication Date Title
EP0951565B1 (en) Improved probing of specific nucleic acids
US7230092B2 (en) Capture moieties for nucleic acids and uses thereof
CN1863927B (zh) 核酸检测分析
US7074564B2 (en) Rolling circle replication of padlock probes
EP1090138A1 (en) Nucleotide detection method
AU772995B2 (en) Methods of synthesizing polynucleotides by ligation of multiple oligomers
WO2002024955A2 (en) Detection of unlabeled hybridized dna and rna using restriction enzyme digestion
WO2000029624A2 (en) Methods and compositions for detection of nucleic acid sequences and signal amplification
EP2561098B1 (en) Tethered enzyme mediated nucleic acid detection
WO2000009738A9 (en) Rolling circle-based analysis of polynucleotide sequence
AU2004270756B2 (en) Nucleic acid detection assay
US7001726B1 (en) Enzymatically catalysed signal amplification

Legal Events

Date Code Title Description
EEER Examination request
MKEX Expiry

Effective date: 20170501