CA2252817C - Improved probing of specific nucleic acids - Google Patents

Improved probing of specific nucleic acids Download PDF

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Publication number
CA2252817C
CA2252817C CA002252817A CA2252817A CA2252817C CA 2252817 C CA2252817 C CA 2252817C CA 002252817 A CA002252817 A CA 002252817A CA 2252817 A CA2252817 A CA 2252817A CA 2252817 C CA2252817 C CA 2252817C
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CA
Canada
Prior art keywords
probe
solid phase
nucleic acid
padlock
target nucleic
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
CA002252817A
Other languages
English (en)
French (fr)
Other versions
CA2252817A1 (en
Inventor
Ulf Landegren
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Olink Holding AB
Original Assignee
Olink AB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Olink AB filed Critical Olink AB
Publication of CA2252817A1 publication Critical patent/CA2252817A1/en
Application granted granted Critical
Publication of CA2252817C publication Critical patent/CA2252817C/en
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6816—Hybridisation assays characterised by the detection means
    • C12Q1/6823—Release of bound markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6834—Enzymatic or biochemical coupling of nucleic acids to a solid phase

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
CA002252817A 1996-04-30 1997-04-30 Improved probing of specific nucleic acids Expired - Lifetime CA2252817C (en)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
SE9601676-1 1996-04-30
SE9601676A SE9601676D0 (sv) 1996-04-30 1996-04-30 Improved probing of specific mucleic acids
PCT/SE1997/000737 WO1997041254A1 (en) 1996-04-30 1997-04-30 Improved probing of specific nucleic acids

Publications (2)

Publication Number Publication Date
CA2252817A1 CA2252817A1 (en) 1997-11-06
CA2252817C true CA2252817C (en) 2009-07-07

Family

ID=20402429

Family Applications (1)

Application Number Title Priority Date Filing Date
CA002252817A Expired - Lifetime CA2252817C (en) 1996-04-30 1997-04-30 Improved probing of specific nucleic acids

Country Status (8)

Country Link
US (2) US20010007742A1 (enExample)
EP (1) EP0951565B1 (enExample)
JP (1) JP2000508920A (enExample)
AT (1) ATE233826T1 (enExample)
CA (1) CA2252817C (enExample)
DE (1) DE69719557T2 (enExample)
SE (1) SE9601676D0 (enExample)
WO (1) WO1997041254A1 (enExample)

Families Citing this family (82)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1994003624A1 (en) 1992-08-04 1994-02-17 Auerbach Jeffrey I Methods for the isothermal amplification of nucleic acid molecules
US6261808B1 (en) * 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
EP1572974A2 (en) * 2002-11-19 2005-09-14 Applera Corporation Polynucleotide sequence detection assays and analysis
WO2004046344A2 (en) * 2002-11-19 2004-06-03 Applera Corporation Polynucleotide sequence detection assays
ATE476523T1 (de) 2003-06-17 2010-08-15 Human Genetic Signatures Pty Verfahren zur genomamplifikation
ATE419394T1 (de) * 2003-09-04 2009-01-15 Human Genetic Signatures Pty Nukleinsäurenachweistest
US8168777B2 (en) 2004-04-29 2012-05-01 Human Genetic Signatures Pty. Ltd. Bisulphite reagent treatment of nucleic acid
US7618780B2 (en) * 2004-05-20 2009-11-17 Trillion Genomics Limited Use of mass labelled probes to detect target nucleic acids using mass spectrometry
DE602005022740D1 (de) 2004-09-10 2010-09-16 Human Genetic Signatures Pty Amplifikationsblocker umfassend interkalierende nukleinsäuren (ina) enthaltend interkalierende pseudonukleotide (ipn)
US7833942B2 (en) 2004-12-03 2010-11-16 Human Genetic Signatures Pty. Ltd. Methods for simplifying microbial nucleic acids by chemical modification of cytosines
US8431347B2 (en) 2005-05-26 2013-04-30 Human Genetic Signatures Pty Ltd Isothermal strand displacement amplification using primers containing a non-regular base
US8343738B2 (en) 2005-09-14 2013-01-01 Human Genetic Signatures Pty. Ltd. Assay for screening for potential cervical cancer
EP1879377A3 (en) * 2006-07-13 2010-06-16 Panasonic Corporation Portable Device
WO2009067743A1 (en) 2007-11-27 2009-06-04 Human Genetic Signatures Pty Ltd Enzymes for amplification and copying bisulphite modified nucleic acids
WO2009121021A1 (en) * 2008-03-28 2009-10-01 Life Technologies Corporation Degradable arrays for preventing reuse
US8470542B2 (en) 2008-12-01 2013-06-25 Laboratory Corporation Of America Holdings Methods and assays for measuring p95 and/or p95 complexes in a sample and antibodies specific for p95
EP2387717B1 (en) * 2009-01-15 2014-12-10 Laboratory Corporation of America Holdings Methods of determining patient response by measurement of her-2 expression
BRPI1007321A2 (en) 2009-01-15 2018-07-10 Laboratory Corporation Of America Holdings methods for measuring and / or quantifying the presence and / or amount of her-3 or her-3 in a complex in a patient sample, and to determine if an individual with cancer is likely to respond to treatment with a targeted therapy. and antibody.
WO2010126614A2 (en) 2009-04-30 2010-11-04 Good Start Genetics, Inc. Methods and compositions for evaluating genetic markers
US12129514B2 (en) 2009-04-30 2024-10-29 Molecular Loop Biosolutions, Llc Methods and compositions for evaluating genetic markers
WO2011066476A1 (en) * 2009-11-25 2011-06-03 Quantalife, Inc. Methods and compositions for detecting genetic material
US9163281B2 (en) 2010-12-23 2015-10-20 Good Start Genetics, Inc. Methods for maintaining the integrity and identification of a nucleic acid template in a multiplex sequencing reaction
CN103562407A (zh) 2011-02-09 2014-02-05 伯乐生命医学产品有限公司 核酸的分析
US9556473B2 (en) 2011-02-15 2017-01-31 Leica Biosystems Newcastle Ltd Methods for identifying nucleic acid sequences
JP5881746B2 (ja) 2011-02-15 2016-03-09 ライカ バイオシステムズ ニューキャッスル リミテッド ｍＲＮＡの局在インサイチュー検出のための方法
US8759036B2 (en) 2011-03-21 2014-06-24 Affymetrix, Inc. Methods for synthesizing pools of probes
US8932819B2 (en) 2011-07-06 2015-01-13 Affymetrix, Inc. Assays for affinity profiling of nucleic acid binding proteins
AU2011376698B2 (en) 2011-09-07 2014-07-17 Human Genetic Signatures Pty Ltd Molecular detection assay
CA2852665A1 (en) 2011-10-17 2013-04-25 Good Start Genetics, Inc. Analysis methods
US8209130B1 (en) 2012-04-04 2012-06-26 Good Start Genetics, Inc. Sequence assembly
US8812422B2 (en) 2012-04-09 2014-08-19 Good Start Genetics, Inc. Variant database
US10227635B2 (en) 2012-04-16 2019-03-12 Molecular Loop Biosolutions, Llc Capture reactions
US10114015B2 (en) 2013-03-13 2018-10-30 Meso Scale Technologies, Llc. Assay methods
WO2014165061A1 (en) 2013-03-13 2014-10-09 Meso Scale Technologies, Llc. Improved assay methods
WO2014152421A1 (en) 2013-03-14 2014-09-25 Good Start Genetics, Inc. Methods for analyzing nucleic acids
BR112015023775B1 (pt) 2013-03-15 2022-08-16 Lubrizol Advanced Materials, Inc Composições de cpvc isentas de metal pesado
US8847799B1 (en) 2013-06-03 2014-09-30 Good Start Genetics, Inc. Methods and systems for storing sequence read data
US9944998B2 (en) 2013-07-25 2018-04-17 Bio-Rad Laboratories, Inc. Genetic assays
WO2015057565A1 (en) 2013-10-18 2015-04-23 Good Start Genetics, Inc. Methods for assessing a genomic region of a subject
US10851414B2 (en) 2013-10-18 2020-12-01 Good Start Genetics, Inc. Methods for determining carrier status
WO2015175530A1 (en) 2014-05-12 2015-11-19 Gore Athurva Methods for detecting aneuploidy
CN112239774B (zh) 2014-05-15 2024-12-03 中尺度技术有限责任公司 改进的测定方法
US10655188B2 (en) 2014-06-13 2020-05-19 Q-Linea Ab Method for determining the identity and antimicrobial susceptibility of a microorganism
WO2016025818A1 (en) 2014-08-15 2016-02-18 Good Start Genetics, Inc. Systems and methods for genetic analysis
US11408024B2 (en) 2014-09-10 2022-08-09 Molecular Loop Biosciences, Inc. Methods for selectively suppressing non-target sequences
CA2999708A1 (en) 2014-09-24 2016-03-31 Good Start Genetics, Inc. Process control for increased robustness of genetic assays
WO2016065207A1 (en) 2014-10-23 2016-04-28 Ricardo Mancebo Reagents and methods for isothermal chain reaction
EP3271480B8 (en) 2015-01-06 2022-09-28 Molecular Loop Biosciences, Inc. Screening for structural variants
GB201507026D0 (en) 2015-04-24 2015-06-10 Linea Ab Q Medical sample transportation container
EP3329010B1 (en) 2015-07-29 2019-07-10 Progenity, Inc. Nucleic acids and methods for detecting chromosomal abnormalities
US20170298427A1 (en) 2015-11-16 2017-10-19 Progenity, Inc. Nucleic acids and methods for detecting methylation status
GB2554767A (en) 2016-04-21 2018-04-11 Q Linea Ab Detecting and characterising a microorganism
WO2018102759A1 (en) 2016-12-01 2018-06-07 Ignite Biosciences, Inc. Methods of assaying proteins
US11661624B2 (en) 2017-03-30 2023-05-30 Pioneer Hi-Bred International, Inc. Methods of identifying and characterizing gene editing variations in nucleic acids
JP7458678B2 (ja) 2017-12-29 2024-04-01 ノーティラス・サブシディアリー・インコーポレイテッド タンパク質同定のためのデコーディングアプローチ方法
JP7348603B2 (ja) 2018-04-02 2023-09-21 エニュメラ・モレキュラー・インコーポレイテッド 核酸分子を計数するための方法、システム、および組成物
EP3931201A1 (en) 2019-03-01 2022-01-05 Meso Scale Technologies, LLC Electrochemiluminescent labeled probes for use in immunoassay methods, methods using such and kits comprising same
WO2020206170A1 (en) 2019-04-02 2020-10-08 Progenity, Inc. Methods, systems, and compositions for counting nucleic acid molecules
JP7727628B2 (ja) 2019-12-03 2025-08-21 アラマー バイオサイエンシーズ， インク． 核酸結合免疫サンドイッチアッセイ（ｎｕｌｉｓａ）
GB202004469D0 (en) 2020-03-27 2020-05-13 Olink Proteomics Ab Controls for proximity detection assays
WO2021252800A1 (en) 2020-06-11 2021-12-16 Nautilus Biotechnology, Inc. Methods and systems for computational decoding of biological, chemical, and physical entities
CA3195721A1 (en) 2020-09-21 2022-03-24 Progenity, Inc. Compositions and methods for isolation of cell-free dna
CA3203106A1 (en) 2021-01-20 2022-07-28 Parag Mallick Systems and methods for biomolecule quantitation
US20220227890A1 (en) 2021-01-21 2022-07-21 Nautilus Biotechnology, Inc. Systems and methods for biomolecule preparation
IL305336B2 (en) 2021-03-11 2025-09-01 Nautilus Subsidiary Inc Systems and methods for preserving biomolecules
US12529049B2 (en) 2021-09-09 2026-01-20 Nautilus Subsidiary, Inc. Characterization and localization of protein modifications
CA3227592A1 (en) 2021-09-22 2023-03-30 Gregory KAPP Methods and systems for determining polypeptide interactions
WO2023081728A1 (en) 2021-11-03 2023-05-11 Nautilus Biotechnology, Inc. Systems and methods for surface structuring
AU2023241893A1 (en) 2022-03-29 2024-09-26 Nautilus Subsidiary, Inc. Integrated arrays for single-analyte processes
US20230360732A1 (en) 2022-04-25 2023-11-09 Nautilus Subsidiary, Inc. Systems and methods for assessing and improving the quality of multiplex molecular assays
WO2023250364A1 (en) 2022-06-21 2023-12-28 Nautilus Subsidiary, Inc. Method for detecting analytes at sites of optically non-resolvable distances
US20240094215A1 (en) 2022-09-15 2024-03-21 Nautilus Subsidiary, Inc. Characterizing accessibility of macromolecule structures
WO2024073599A1 (en) 2022-09-29 2024-04-04 Nautilus Subsidiary, Inc. Preparation of array surfaces for single-analyte processes
US20240192202A1 (en) 2022-12-09 2024-06-13 Nautilus Subsidiary, Inc. Fluidic media for single-analtye arrays
EP4634285A1 (en) 2022-12-15 2025-10-22 Nautilus Subsidiary, Inc. Inhibition of photon phenomena on single molecule arrays
US20240344116A1 (en) 2023-03-24 2024-10-17 Nautilus Subsidiary, Inc. Transfer of nanoparticles to array surfaces
WO2024243237A1 (en) 2023-05-24 2024-11-28 Nautilus Subsidiary, Inc. Systems and methods for machine vision
WO2025043159A1 (en) 2023-08-24 2025-02-27 Nautilus Subsidiary, Inc. Compositions and methods for detecting binding interactions under equilibrium or non-equilibrium conditions
WO2025128588A1 (en) 2023-12-11 2025-06-19 Nautilus Subsidiary, Inc. Methods and compositions of particle-based arrays
WO2025137166A1 (en) 2023-12-20 2025-06-26 Nautilus Subsidiary, Inc. Fluidic devices for single-analyte assays
WO2025199236A1 (en) 2024-03-22 2025-09-25 Nautilus Subsidiary, Inc. Plasmonic particle systems for single-analyte assays
WO2025212338A1 (en) 2024-04-01 2025-10-09 Nautilus Subsidiary, Inc. Nanostructures for modulation of analyte conformation

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5124246A (en) * 1987-10-15 1992-06-23 Chiron Corporation Nucleic acid multimers and amplified nucleic acid hybridization assays using same
US5427930A (en) * 1990-01-26 1995-06-27 Abbott Laboratories Amplification of target nucleic acids using gap filling ligase chain reaction
SE9400522D0 (sv) * 1994-02-16 1994-02-16 Ulf Landegren Method and reagent for detecting specific nucleotide sequences
US5643724A (en) * 1994-06-06 1997-07-01 Roche Molecular Systems, Inc. Methods and reagents for Glycophorin A typing
GB9414934D0 (en) * 1994-07-25 1994-09-14 Raggio Italgene Spa Method and reagents for the detection of target nuceic acid sequences
US5952176A (en) 1995-07-11 1999-09-14 Forfas (Trading As Bioresearch Ireland) Glycosylase mediated detection of nucleotide sequences at candidate loci

Also Published As

Publication number Publication date
US20020102592A1 (en) 2002-08-01
WO1997041254A1 (en) 1997-11-06
DE69719557T2 (de) 2004-02-26
DE69719557D1 (de) 2003-04-10
EP0951565A1 (en) 1999-10-27
US20010007742A1 (en) 2001-07-12
US7351528B2 (en) 2008-04-01
ATE233826T1 (de) 2003-03-15
EP0951565B1 (en) 2003-03-05
CA2252817A1 (en) 1997-11-06
JP2000508920A (ja) 2000-07-18
SE9601676D0 (sv) 1996-04-30

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Effective date: 20170501