DE69220084T2 - Diagnostische Anwendungen der Bildung von doppelten D-Loops. - Google Patents

Diagnostische Anwendungen der Bildung von doppelten D-Loops.

Info

Publication number
DE69220084T2
DE69220084T2 DE69220084T DE69220084T DE69220084T2 DE 69220084 T2 DE69220084 T2 DE 69220084T2 DE 69220084 T DE69220084 T DE 69220084T DE 69220084 T DE69220084 T DE 69220084T DE 69220084 T2 DE69220084 T2 DE 69220084T2
Authority
DE
Germany
Prior art keywords
dna
probe
target
probes
reca
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Fee Related
Application number
DE69220084T
Other languages
German (de)
English (en)
Other versions
DE69220084D1 (de
Inventor
Cornelia Calhoun
Elissa Sena
David Zarling
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Tapestry Pharmaceuticals Inc
Original Assignee
Daikin Industries Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US07/520,321 external-priority patent/US5223414A/en
Priority claimed from US07/755,462 external-priority patent/US5273881A/en
Application filed by Daikin Industries Ltd filed Critical Daikin Industries Ltd
Application granted granted Critical
Publication of DE69220084D1 publication Critical patent/DE69220084D1/de
Publication of DE69220084T2 publication Critical patent/DE69220084T2/de
Anticipated expiration legal-status Critical
Expired - Fee Related legal-status Critical Current

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6816Hybridisation assays characterised by the detection means
    • C12Q1/682Signal amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/10Processes for the isolation, preparation or purification of DNA or RNA
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6827Hybridisation assays for detection of mutation or polymorphism
    • C12Q1/683Hybridisation assays for detection of mutation or polymorphism involving restriction enzymes, e.g. restriction fragment length polymorphism [RFLP]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Biomedical Technology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Stabilization Of Oscillater, Synchronisation, Frequency Synthesizers (AREA)
  • Magnetic Resonance Imaging Apparatus (AREA)
  • Saccharide Compounds (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Measurement Of The Respiration, Hearing Ability, Form, And Blood Characteristics Of Living Organisms (AREA)
DE69220084T 1990-05-07 1992-09-04 Diagnostische Anwendungen der Bildung von doppelten D-Loops. Expired - Fee Related DE69220084T2 (de)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US07/520,321 US5223414A (en) 1990-05-07 1990-05-07 Process for nucleic acid hybridization and amplification
US07/755,462 US5273881A (en) 1990-05-07 1991-09-04 Diagnostic applications of double D-loop formation
US91079192A 1992-07-09 1992-07-09
PCT/JP1992/001135 WO1993005178A1 (en) 1990-05-07 1992-09-04 Diagnostic applications of double d-loop formation

Publications (2)

Publication Number Publication Date
DE69220084D1 DE69220084D1 (de) 1997-07-03
DE69220084T2 true DE69220084T2 (de) 1997-09-11

Family

ID=27414753

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69220084T Expired - Fee Related DE69220084T2 (de) 1990-05-07 1992-09-04 Diagnostische Anwendungen der Bildung von doppelten D-Loops.

Country Status (13)

Country Link
US (1) US5670316A (enExample)
EP (1) EP0612352B1 (enExample)
JP (1) JP2745816B2 (enExample)
KR (1) KR100245284B1 (enExample)
AT (1) ATE153707T1 (enExample)
AU (1) AU661505B2 (enExample)
CA (1) CA2116215C (enExample)
DE (1) DE69220084T2 (enExample)
DK (1) DK0612352T3 (enExample)
ES (1) ES2101867T3 (enExample)
FI (1) FI941016L (enExample)
NO (1) NO313557B1 (enExample)
WO (1) WO1993005178A1 (enExample)

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102017114537A1 (de) * 2017-06-29 2019-01-03 Endress+Hauser Conducta Gmbh+Co. Kg Sensormembran, Sensorkappe und optischer Sensor

Families Citing this family (68)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5506098A (en) * 1991-09-04 1996-04-09 Daikin Industries, Ltd. In situ hybridization method
WO1993022443A1 (en) * 1992-04-24 1993-11-11 Sri International In vivo homologous sequence targeting in eukaryotic cells
US5929043A (en) * 1995-01-31 1999-07-27 Pangene Corporation Recombinase mediated DNA therapies
FR2737502B1 (fr) * 1995-07-31 1997-10-24 Genset Sa Procede de detection d'acides nucleiques utilisant des sondes nucleotidiques permettant a la fois une capture specifique et une detection
WO1998008975A1 (en) * 1996-08-29 1998-03-05 Daikin Industries, Ltd. Methods for targeting, enriching, detecting and/or isolating target nucleic acid sequence using reca-like recombinase
JP3891620B2 (ja) * 1996-11-14 2007-03-14 株式会社アイシン・コスモス研究所 ヘアピン型構造の核酸プローブ分子、及び該核酸プローブ分子を利用した核酸検出方法
US5948653A (en) * 1997-03-21 1999-09-07 Pati; Sushma Sequence alterations using homologous recombination
US6342350B1 (en) * 1997-09-05 2002-01-29 The General Hospital Corporation Alpha-2-macroglobulin diagnostic test
EP1038017A2 (en) 1997-12-11 2000-09-27 Pangene Corporation The use of consensus sequences for targeted homologous gene isolation and recombination in gene families
DE19980548D2 (de) * 1998-04-01 2001-07-12 Juergen Wolfrum Verfahren und Vorrichtung zur Quantifizierung von DNA und RNA
US6287772B1 (en) 1998-04-29 2001-09-11 Boston Probes, Inc. Methods, kits and compositions for detecting and quantitating target sequences
US6541227B1 (en) * 1998-10-12 2003-04-01 Aisin Seiki Kabushiki Kaisha Preparation of labeled DNA
JP4265028B2 (ja) * 1999-04-14 2009-05-20 アイシン精機株式会社 二本鎖dnaの特異的切断方法およびそのためのキット
AU4657500A (en) 1999-04-21 2000-11-02 Pangene Corporation Locked nucleic acid hybrids and methods of use
DE60042370D1 (de) * 1999-12-21 2009-07-23 Ortho Clinical Diagnostics Inc Detektion von nukleinsäuren
CA2397357A1 (en) * 1999-12-28 2001-07-05 David A. Zarling Recombinase mediated gene chip detection
US20020132259A1 (en) * 2001-02-21 2002-09-19 Wagner Robert E. Mutation detection using MutS and RecA
WO2002079495A2 (en) * 2001-03-27 2002-10-10 University Of Delaware Genomics applications for modified oligonucleotides
DE60226856D1 (de) 2001-04-20 2008-07-10 Penn State Res Found Verfahren zur manipulation von nukleinsäuren
US7468244B2 (en) * 2001-09-27 2008-12-23 University Of Delaware Polymorphism detection and separation
AU2002341898A2 (en) * 2001-09-28 2003-04-07 University Of Delaware Polymorphism detection and separation
US7244562B2 (en) * 2001-11-01 2007-07-17 Gene Check, Inc. RecA assisted detection of mutations, single nucleotide polymorphisms and specific sequences
AU2003215391B2 (en) * 2002-02-21 2007-05-24 Abbott Diagnostics Scarborough, Inc. Recombinase Polymerase Amplification
US8030000B2 (en) 2002-02-21 2011-10-04 Alere San Diego, Inc. Recombinase polymerase amplification
US7399590B2 (en) * 2002-02-21 2008-07-15 Asm Scientific, Inc. Recombinase polymerase amplification
WO2003100095A1 (en) * 2002-05-08 2003-12-04 Arkray, Inc. Method of detecting target nucleic acid
WO2004007692A2 (en) * 2002-07-17 2004-01-22 U.S.Genomics, Inc. Methods and compositions for analyzing polymers using chimeric tags
WO2004081224A2 (en) * 2003-03-11 2004-09-23 Gene Check, Inc. Reca-assisted allele specific oligonucleotide extension method
JP2007501003A (ja) * 2003-08-01 2007-01-25 ユー.エス. ジェノミクス, インコーポレイテッド 非切断条件下で核酸を分析するための配列特異的エンドヌクレアーゼの使用に関連した方法および組成物
WO2005017205A2 (en) * 2003-08-04 2005-02-24 U. S. Genomics, Inc. Nucleic acid mapping using linear analysis
JP5026958B2 (ja) * 2004-06-01 2012-09-19 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅
JP2006055065A (ja) * 2004-08-19 2006-03-02 Toyo Kohan Co Ltd 非対称pcrを用いた核酸の検出方法、ポリヌクレオチドプローブ、それを固定するプローブ固定化担体およびキット
WO2006051988A1 (ja) * 2004-11-15 2006-05-18 Riken 核酸の増幅方法
CA2616241C (en) 2005-07-25 2012-02-07 Asm Scientific, Inc. Methods for multiplexing recombinase polymerase amplification
US20090064377A1 (en) * 2005-09-29 2009-03-05 Daphne Yvette Rainey-Wittich Method and means for targeted nucleotide exchange
US20080003600A1 (en) * 2006-02-27 2008-01-03 Salinas Frank G Isothermal screening of breast cancer related nucleic acid
US20080220421A1 (en) * 2006-02-27 2008-09-11 Salinas Frank G Isothermal screening for nucleic acids associated with diseases and conditions of the gi tract
US20070202522A1 (en) * 2006-02-27 2007-08-30 Salinas Frank G Isothermal screening of tumor cell related nucleic acids
US20090061413A1 (en) * 2006-02-27 2009-03-05 Salinas Frank G Isothermal screening of hiv-1 related nucleic acids
CA2650993C (en) 2006-05-04 2015-06-16 Asm Scientific, Inc. Recombinase polymerase amplification
WO2010135310A1 (en) 2009-05-20 2010-11-25 Biosite Incorporated Dna glycosylase/lyase and ap endonuclease substrates
EP2438196B1 (en) 2009-06-05 2016-12-21 Alere San Diego, Inc. Recombinase polymerase amplification reagents and kits
JP5795341B2 (ja) 2010-03-08 2015-10-14 デイナ ファーバー キャンサー インスティチュート,インコーポレイテッド 参照ブロック配列によるfullCOLD−PCR濃縮
CA2830361C (en) 2011-03-31 2021-03-16 Dana-Farber Cancer Institute, Inc. Methods and compositions to enable multiplex cold-pcr
JP5961247B2 (ja) 2011-04-07 2016-08-02 アリーア サン ディエゴ, インコーポレイテッド リコンビナーゼポリメラーゼ増幅混合物のモニタリング
EP3461910B1 (en) 2012-04-19 2020-08-26 Life Technologies Corporation Nucleic acid amplification
SG11201406717RA (en) 2012-04-19 2014-11-27 Life Technologies Corp Nucleic acid amplification
US20150197787A1 (en) * 2012-08-02 2015-07-16 Qiagen Gmbh Recombinase mediated targeted dna enrichment for next generation sequencing
WO2014043143A1 (en) 2012-09-11 2014-03-20 Life Technologies Corporation Nucleic acid amplification
EP2895620B1 (en) 2012-09-11 2017-08-02 Life Technologies Corporation Nucleic acid amplification
CN106103713B (zh) 2014-02-03 2021-05-28 赛默飞世尔科技波罗的海封闭股份公司 用于经控制dna片段化的方法
EP3155127B1 (en) 2014-06-13 2020-07-22 Life Technologies Corporation Multiplex nucleic acid amplification
US10344336B2 (en) 2015-06-09 2019-07-09 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
CA2990846C (en) * 2015-06-24 2023-09-26 Dana-Farber Cancer Institute, Inc. Selective degradation of wild-type dna and enrichment of mutant alleles using nuclease
WO2017100283A1 (en) 2015-12-09 2017-06-15 Life Technologies Corporation Detection and quantification of nucleic acid molecules associated with a surface
EP3402880B1 (en) 2016-01-15 2024-01-03 Thermo Fisher Scientific Baltics UAB Thermophilic dna polymerase mutants
US11268117B2 (en) 2016-06-10 2022-03-08 Life Technologies Corporation Methods and compositions for nucleic acid amplification
WO2018071522A1 (en) 2016-10-11 2018-04-19 Life Technologies Corporation Rapid amplification of nucleic acids
US11371090B2 (en) 2016-12-12 2022-06-28 Dana-Farber Cancer Institute, Inc. Compositions and methods for molecular barcoding of DNA molecules prior to mutation enrichment and/or mutation detection
US11542540B2 (en) 2017-06-16 2023-01-03 Life Technologies Corporation Control nucleic acids, and compositions, kits, and uses thereof
WO2019002178A1 (en) 2017-06-26 2019-01-03 Thermo Fisher Scientific Baltics Uab DNA MUTANTS THERMOPHILIC POLYMERASES
US12226745B2 (en) 2017-11-07 2025-02-18 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
EP4310196A3 (en) 2017-11-07 2024-04-24 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
EP4403648B1 (en) 2019-05-03 2025-08-20 Life Technologies Corporation Methods and compositions for manipulating nucleic acids
KR20220062302A (ko) 2019-08-21 2022-05-16 라이프 테크놀로지스 코포레이션 시퀀싱을 위한 시스템 및 방법
US20220170093A1 (en) 2020-11-16 2022-06-02 Life Technologies Corporation System and method for sequencing
NL2033124B1 (en) * 2022-09-23 2024-03-29 Rapidemic B V Methods and device for multiple-label nucleic acid amplification and detection
WO2025144744A1 (en) 2023-12-29 2025-07-03 Life Technologies Corporation Enrichment of clonal substrates

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI63596C (fi) * 1981-10-16 1983-07-11 Orion Yhtymae Oy Mikrobdiagnostiskt foerfarande som grundar sig pao skiktshybridisering av nukleinsyror och vid foerfarandet anvaenda kombinationer av reagenser
US4766062A (en) * 1984-05-07 1988-08-23 Allied Corporation Displacement polynucleotide assay method and polynucleotide complex reagent therefor
CA1254114A (en) * 1984-05-07 1989-05-16 Allied Corporation Displacement polynucleotide assay employing recombination protein and diagnostic kit
JPS61502282A (ja) * 1984-06-01 1986-10-09 ナショナル・バイオメディカル・リサ−チ・ファウンデ−ション 酵素剤を用いた触媒作用による核酸交雑方法
US4683195A (en) * 1986-01-30 1987-07-28 Cetus Corporation Process for amplifying, detecting, and/or-cloning nucleic acid sequences
US4683202A (en) * 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US4888274A (en) * 1985-09-18 1989-12-19 Yale University RecA nucleoprotein filament and methods
EP0236491A1 (en) * 1985-09-18 1987-09-16 Yale University RecA NUCLEOPROTEIN FILAMENT AND METHODS
AU2735988A (en) * 1987-12-21 1989-07-13 Amoco Corporation Target and background capture methods with amplification for affinity assays
EP0322311B1 (en) * 1987-12-21 1994-03-23 Applied Biosystems, Inc. Method and kit for detecting a nucleic acid sequence
US5273881A (en) * 1990-05-07 1993-12-28 Daikin Industries, Ltd. Diagnostic applications of double D-loop formation
US5223414A (en) * 1990-05-07 1993-06-29 Sri International Process for nucleic acid hybridization and amplification
US5506098A (en) * 1991-09-04 1996-04-09 Daikin Industries, Ltd. In situ hybridization method

Cited By (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102017114537A1 (de) * 2017-06-29 2019-01-03 Endress+Hauser Conducta Gmbh+Co. Kg Sensormembran, Sensorkappe und optischer Sensor

Also Published As

Publication number Publication date
EP0612352B1 (en) 1997-05-28
NO940744L (enExample) 1994-05-02
AU2540192A (en) 1993-04-05
ATE153707T1 (de) 1997-06-15
DK0612352T3 (da) 1997-06-30
WO1993005178A1 (en) 1993-03-18
NO940744D0 (no) 1994-03-03
JPH06510201A (ja) 1994-11-17
JP2745816B2 (ja) 1998-04-28
FI941016A0 (fi) 1994-03-03
NO313557B1 (no) 2002-10-21
FI941016A7 (fi) 1994-05-03
FI941016L (fi) 1994-05-03
DE69220084D1 (de) 1997-07-03
ES2101867T3 (es) 1997-07-16
KR100245284B1 (ko) 2000-03-02
EP0612352A1 (en) 1994-08-31
CA2116215A1 (en) 1993-03-05
AU661505B2 (en) 1995-07-27
US5670316A (en) 1997-09-23
CA2116215C (en) 1998-12-22

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Legal Events

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8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: NAPRO BIO THERAPEUTICS.INC., NEWARK, DEL., US

8328 Change in the person/name/address of the agent

Representative=s name: VOSSIUS & PARTNER, 81675 MUENCHEN

8327 Change in the person/name/address of the patent owner

Owner name: TAPESTRY PHARMACEUTICALS, INC. (N.D.GES.D. STAATES

8339 Ceased/non-payment of the annual fee