DE60233734D1 - Verfahren zur herstellung vom glucagon-ähnlichen peptide 1 (glp 1)7-36 und eines glp-1-analogs - Google Patents

Verfahren zur herstellung vom glucagon-ähnlichen peptide 1 (glp 1)7-36 und eines glp-1-analogs

Info

Publication number
DE60233734D1
DE60233734D1 DE60233734T DE60233734T DE60233734D1 DE 60233734 D1 DE60233734 D1 DE 60233734D1 DE 60233734 T DE60233734 T DE 60233734T DE 60233734 T DE60233734 T DE 60233734T DE 60233734 D1 DE60233734 D1 DE 60233734D1
Authority
DE
Germany
Prior art keywords
glp
colibracillus
genes
promoting
glucagon
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60233734T
Other languages
English (en)
Inventor
Yukun Sun
Dengxi Wu
Aizhen Wu
Zhiyong Zhu
Gang Yu
Jiaxiang Zhou
Shaoling Zhao
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
SHANGHAI HUA-YI BIO-TECH LAB
SHANGHAI HUA YI BIO TECH LAB
Original Assignee
SHANGHAI HUA-YI BIO-TECH LAB
SHANGHAI HUA YI BIO TECH LAB
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by SHANGHAI HUA-YI BIO-TECH LAB, SHANGHAI HUA YI BIO TECH LAB filed Critical SHANGHAI HUA-YI BIO-TECH LAB
Application granted granted Critical
Publication of DE60233734D1 publication Critical patent/DE60233734D1/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60233734T 2001-07-19 2002-07-17 Verfahren zur herstellung vom glucagon-ähnlichen peptide 1 (glp 1)7-36 und eines glp-1-analogs Expired - Lifetime DE60233734D1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN01126278A CN1363654A (zh) 2001-07-19 2001-07-19 生产促胰岛素分泌肽glp－1(7－36)的基因工程菌以及生产glp－1(7－36)的方法
PCT/CN2002/000502 WO2003016349A1 (fr) 2001-07-19 2002-07-17 Methode de production d'un peptide 1 de type glucagon (glp-1) 7-36 et d'un analogue de glp-1

Publications (1)

Publication Number Publication Date
DE60233734D1 true DE60233734D1 (de) 2009-10-29

Family

ID=4666300

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60233734T Expired - Lifetime DE60233734D1 (de) 2001-07-19 2002-07-17 Verfahren zur herstellung vom glucagon-ähnlichen peptide 1 (glp 1)7-36 und eines glp-1-analogs

Country Status (13)

Country Link
US (1) US7544512B2 (de)
EP (1) EP1408050B1 (de)
JP (1) JP4504014B2 (de)
KR (1) KR100959549B1 (de)
CN (2) CN1363654A (de)
AT (1) ATE443081T1 (de)
AU (1) AU2002313869B2 (de)
BR (1) BR0211435B1 (de)
CA (1) CA2454264C (de)
DE (1) DE60233734D1 (de)
DK (1) DK1408050T3 (de)
ES (1) ES2333413T3 (de)
WO (1) WO2003016349A1 (de)

Families Citing this family (24)

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Publication number Priority date Publication date Assignee Title
CN1162446C (zh) 2001-05-10 2004-08-18 上海华谊生物技术有限公司 促胰岛素分泌肽衍生物
US7176278B2 (en) 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
US8129504B2 (en) 2001-08-30 2012-03-06 Biorexis Technology, Inc. Oral delivery of modified transferrin fusion proteins
EP1626981A4 (de) * 2003-03-04 2006-11-22 Biorexis Pharmaceutical Corp Dipeptidylpeptidase-geschützte proteine
CN100392070C (zh) * 2004-09-08 2008-06-04 华东师范大学 表达人胰高血糖素样肽-1的基因工程菌及其构建方法和应用
JPWO2006030492A1 (ja) * 2004-09-14 2008-07-31 日本製紙株式会社 Ｇｌｐ−１誘導体が集積された植物及び植物貯蔵器官とその生産方法
US7893017B2 (en) * 2004-10-07 2011-02-22 Novo Nordisk A/S Protracted GLP-1 compounds
EP2330126B1 (de) 2004-10-07 2015-12-23 Novo Nordisk A/S Exendin-4-Zusammensetzungen mit verlängerter Halbwertszeit
ES2336575T3 (es) 2005-09-22 2010-04-14 Biocompatibles Uk Limited Polipeptidos de fusion glp-1 (peptido-1 similar al glucagon) con resistencia aumentada a la peptidasa.
US8202837B2 (en) * 2005-11-04 2012-06-19 Glaxosmithkline Llc Methods for administering hypoglycemic agents
ATE444741T1 (de) * 2006-05-10 2009-10-15 Biocompatibles Uk Ltd Glp-1 peptide enthaltende kugelförmige mikrokapseln, deren produktion und deren verwendung
PL2038423T3 (pl) * 2006-06-21 2013-05-31 Biocon Ltd Metoda wytwarzania biologicznie aktywnego polipeptydu insulinotropowego
EP2049567A2 (de) 2006-07-18 2009-04-22 Centocor, Inc. Menschliche glp-1-mimetikörper, zusammensetzungen, verfahren und anwendungen
ATE524493T1 (de) 2006-07-24 2011-09-15 Biorexis Pharmaceutical Corp Exendin-fusionsproteine
EP1972349A1 (de) * 2007-03-21 2008-09-24 Biocompatibles UK Limited Mit Polymer(en) konjugierte GLP-1-Fusionspeptide, ihre Herstellung und Verwendung
EP1975176A1 (de) * 2007-03-27 2008-10-01 Biocompatibles UK Limited Neue GLP-1-Fusionspeptide, ihre Herstellung und Verwendung
CN101220088B (zh) * 2007-09-24 2012-01-25 中国人民解放军第四军医大学 人胰高血糖素样肽-1类似物的融合蛋白及其应用
EP2163243A1 (de) * 2008-09-12 2010-03-17 Biocompatibles UK Limited Behandlung akuter Herzinfarkte mithilfe eingekapselter Zellen, welche GLP-1-Peptide oder deren Analoga kodieren und absondern
CN102093475B (zh) * 2009-12-11 2014-02-26 吴晓琰 胰高血糖素样肽-1的类似物
CN102242127B (zh) * 2011-05-20 2012-12-12 严杰 串联抗菌肽Alloferon-1的制备方法及rAlloferon-1-K的应用
SG11201405721VA (en) * 2012-03-19 2014-10-30 Madeleine Pharmaceuticals Ρτy Ltd Method of producing a recombinant peptide
CN107586330B (zh) * 2016-07-08 2021-04-16 上海多米瑞生物技术有限公司 替度鲁肽串联多肽及替度鲁肽制备方法
US20220127324A1 (en) * 2019-02-24 2022-04-28 Oncosimis Biotech Private Limited Method for continuous production of recombinant glp-1 peptide by bacteria
TW202307010A (zh) * 2021-06-30 2023-02-16 美商雅利斯塔製藥公司 單體融合肽及其使用方法

Family Cites Families (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5459049A (en) 1989-01-06 1995-10-17 Sanwa Kagaku Kenkyushko Co., Ltd. Motilin-like polypeptide and use thereof
JPH0722517B2 (ja) * 1989-08-24 1995-03-15 株式会社三和化学研究所 モチリン様ポリペプチドの製法並びにそのための組換えｄｎａ及び発現用プラスミド
EP0378078A1 (de) 1989-01-06 1990-07-18 Sanwa Kagaku Kenkyusho Co., Ltd. Dem Motilin ähnliches Polypeptid und seine Verwendung
US5545618A (en) 1990-01-24 1996-08-13 Buckley; Douglas I. GLP-1 analogs useful for diabetes treatment
US5595887A (en) * 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
NZ245015A (en) * 1991-11-05 1995-12-21 Transkaryotic Therapies Inc Delivery of human growth hormone through the administration of transfected cell lines encoding human growth hormone, which are physically protected from host immune response; the transfected cells and their production
JPH06306100A (ja) * 1993-04-26 1994-11-01 Sanwa Kagaku Kenkyusho Co Ltd Ｖｉｐアナログ調製用融合蛋白、ｖｉｐアナログの製法並びにそのための遺伝子組換えプラスミド及び形質転換微生物
US5512459A (en) 1993-07-20 1996-04-30 Bionebraska, Inc. Enzymatic method for modification or recombinant polypeptides
DK144093D0 (de) * 1993-12-23 1993-12-23 Novo Nordisk As
JP2001503963A (ja) * 1996-02-06 2001-03-27 イーライ・リリー・アンド・カンパニー 糖尿病治療
US6006753A (en) * 1996-08-30 1999-12-28 Eli Lilly And Company Use of GLP-1 or analogs to abolish catabolic changes after surgery
US5912327A (en) * 1996-09-30 1999-06-15 Human Genome Sciences, Inc. Method of purifying chemokines from inclusion bodies
US5846774A (en) * 1997-03-21 1998-12-08 Bionebraska, Inc. Chlorella virus promoters
US6395965B1 (en) * 1997-03-21 2002-05-28 Restoragen, Inc. Plant containing a gene construct comprising a chlorella virus promoter and a lac operator
JPH11178574A (ja) * 1997-12-22 1999-07-06 Toyota Central Res & Dev Lab Inc 新規コラーゲン様タンパク質
EP1056774A1 (de) * 1998-02-27 2000-12-06 Novo Nordisk A/S N-terminal verkürzte glp-1 derivate
WO1999043341A1 (en) 1998-02-27 1999-09-02 Novo Nordisk A/S Glp-1 derivatives with helix-content exceeding 25 %, forming partially structured micellar-like aggregates
ATE424413T1 (de) 2000-06-16 2009-03-15 Lilly Co Eli Analoge des glucagon-ähnlichen peptids-1

Also Published As

Publication number Publication date
KR20040097114A (ko) 2004-11-17
CA2454264C (en) 2010-06-22
WO2003016349A1 (fr) 2003-02-27
ATE443081T1 (de) 2009-10-15
JP4504014B2 (ja) 2010-07-14
EP1408050A1 (de) 2004-04-14
CA2454264A1 (en) 2003-02-27
EP1408050A4 (de) 2005-08-10
ES2333413T3 (es) 2010-02-22
CN1363654A (zh) 2002-08-14
DK1408050T3 (da) 2010-01-18
US7544512B2 (en) 2009-06-09
US20040146985A1 (en) 2004-07-29
AU2002313869B2 (en) 2008-04-03
CN100579986C (zh) 2010-01-13
EP1408050B1 (de) 2009-09-16
JP2005515167A (ja) 2005-05-26
CN1531551A (zh) 2004-09-22
BR0211435A (pt) 2004-07-13
KR100959549B1 (ko) 2010-05-27
BR0211435B1 (pt) 2014-12-23

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