WO2003016349A1 - Methode de production d'un peptide 1 de type glucagon (glp-1) 7-36 et d'un analogue de glp-1 - Google Patents

Methode de production d'un peptide 1 de type glucagon (glp-1) 7-36 et d'un analogue de glp-1 Download PDF

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Publication number
WO2003016349A1
WO2003016349A1 PCT/CN2002/000502 CN0200502W WO03016349A1 WO 2003016349 A1 WO2003016349 A1 WO 2003016349A1 CN 0200502 W CN0200502 W CN 0200502W WO 03016349 A1 WO03016349 A1 WO 03016349A1
Authority
WO
WIPO (PCT)
Prior art keywords
glp
analogue
peptide
genes
present
Prior art date
Application number
PCT/CN2002/000502
Other languages
English (en)
French (fr)
Inventor
Yukun Sun
Dengxi Wu
Aizhen Wu
Zhiyong Zhu
Gang Yu
Jiaxiang Zhou
Shaoling Zhao
Original Assignee
Shanghai Hua-Yi Bio-Tech Lab
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority to AT02752955T priority Critical patent/ATE443081T1/de
Priority to CN02814355A priority patent/CN100579986C/zh
Priority to DK02752955.1T priority patent/DK1408050T3/da
Priority to JP2003521271A priority patent/JP4504014B2/ja
Priority to BRPI0211435-6A priority patent/BR0211435B1/pt
Priority to AU2002313869A priority patent/AU2002313869B2/en
Application filed by Shanghai Hua-Yi Bio-Tech Lab filed Critical Shanghai Hua-Yi Bio-Tech Lab
Priority to KR1020047000825A priority patent/KR100959549B1/ko
Priority to EP02752955A priority patent/EP1408050B1/en
Priority to CA2454264A priority patent/CA2454264C/en
Priority to DE60233734T priority patent/DE60233734D1/de
Publication of WO2003016349A1 publication Critical patent/WO2003016349A1/zh
Priority to US10/761,717 priority patent/US7544512B2/en

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/605—Glucagons
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Endocrinology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Toxicology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Microbiology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
PCT/CN2002/000502 2001-07-19 2002-07-17 Methode de production d'un peptide 1 de type glucagon (glp-1) 7-36 et d'un analogue de glp-1 WO2003016349A1 (fr)

Priority Applications (11)

Application Number Priority Date Filing Date Title
CN02814355A CN100579986C (zh) 2001-07-19 2002-07-17 一种生产促胰岛素分泌肽glp-1(7-36)及glp-1类似物的方法
DK02752955.1T DK1408050T3 (da) 2001-07-19 2002-07-17 Fremgangsmåde til fremstilling af glucagon-lignende peptid 1 (GLP-1) 7-36 og en GLP-1-analog
JP2003521271A JP4504014B2 (ja) 2001-07-19 2002-07-17 インスリン分泌性ｇｌｐ−１（７−３６）ポリペプチドおよび／またはｇｌｐ−１類似体を生成する方法
BRPI0211435-6A BR0211435B1 (pt) 2001-07-19 2002-07-17 Método de produção de polipeptídeo glp-1 insulinotrópico (7-36) e/ou análogos de glp
AU2002313869A AU2002313869B2 (en) 2001-07-19 2002-07-17 A method of producing glucagon-like peptide 1(GLP-1)7-36 and an GLP-1 analogue
AT02752955T ATE443081T1 (de) 2001-07-19 2002-07-17 Verfahren zur herstellung vom glucagon-ähnlichen peptide 1 (glp 1)7-36 und eines glp-1-analogs
KR1020047000825A KR100959549B1 (ko) 2001-07-19 2002-07-17 글루카곤 유사 펩타이드 1 glp-1(7-36)과 glp-1 유사체를 생산하는 방법
EP02752955A EP1408050B1 (en) 2001-07-19 2002-07-17 A method of producing glucagon-like peptide 1(glp-1)7-36 and an glp-1 analogue
CA2454264A CA2454264C (en) 2001-07-19 2002-07-17 A method of producing insulinotropic glp-1 (7-36) polypeptide and/or glp-1 analogs
DE60233734T DE60233734D1 (de) 2001-07-19 2002-07-17 Verfahren zur herstellung vom glucagon-ähnlichen peptide 1 (glp 1)7-36 und eines glp-1-analogs
US10/761,717 US7544512B2 (en) 2001-07-19 2004-01-20 Method of producing insulinotropic GLP-1 (7-36) polypeptide and/or GLP-1 analogs

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
CN01126278.8 2001-07-19
CN01126278A CN1363654A (zh) 2001-07-19 2001-07-19 生产促胰岛素分泌肽glp－1(7－36)的基因工程菌以及生产glp－1(7－36)的方法

Related Child Applications (1)

Application Number Title Priority Date Filing Date
US10/761,717 Continuation-In-Part US7544512B2 (en) 2001-07-19 2004-01-20 Method of producing insulinotropic GLP-1 (7-36) polypeptide and/or GLP-1 analogs

Publications (1)

Publication Number Publication Date
WO2003016349A1 true WO2003016349A1 (fr) 2003-02-27

Family

ID=4666300

Family Applications (1)

Application Number Title Priority Date Filing Date
PCT/CN2002/000502 WO2003016349A1 (fr) 2001-07-19 2002-07-17 Methode de production d'un peptide 1 de type glucagon (glp-1) 7-36 et d'un analogue de glp-1

Country Status (13)

Country Link
US (1) US7544512B2 (zh)
EP (1) EP1408050B1 (zh)
JP (1) JP4504014B2 (zh)
KR (1) KR100959549B1 (zh)
CN (2) CN1363654A (zh)
AT (1) ATE443081T1 (zh)
AU (1) AU2002313869B2 (zh)
BR (1) BR0211435B1 (zh)
CA (1) CA2454264C (zh)
DE (1) DE60233734D1 (zh)
DK (1) DK1408050T3 (zh)
ES (1) ES2333413T3 (zh)
WO (1) WO2003016349A1 (zh)

Cited By (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1626981A4 (en) * 2003-03-04 2006-11-22 Biorexis Pharmaceutical Corp PROTEINS PROTECTED AGAINST DIPEPTIDYLPEPTIDASE
US7176278B2 (en) 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
US7329646B2 (en) 2001-05-10 2008-02-12 Shanghai Huayi Bio-Lab Co., Ltd. Derivatives of the insulinotropic peptide exendin-4 and methods of production thereof
US7867972B2 (en) 2006-07-24 2011-01-11 Pharmacia & Upjohn Company, Llc Fusion protein of exendin-4 to a transferrin (Tf) polypeptide
US8129504B2 (en) 2001-08-30 2012-03-06 Biorexis Technology, Inc. Oral delivery of modified transferrin fusion proteins

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN100392070C (zh) * 2004-09-08 2008-06-04 华东师范大学 表达人胰高血糖素样肽-1的基因工程菌及其构建方法和应用
JPWO2006030492A1 (ja) * 2004-09-14 2008-07-31 日本製紙株式会社 Ｇｌｐ−１誘導体が集積された植物及び植物貯蔵器官とその生産方法
US7893017B2 (en) * 2004-10-07 2011-02-22 Novo Nordisk A/S Protracted GLP-1 compounds
EP2330126B1 (en) 2004-10-07 2015-12-23 Novo Nordisk A/S Protracted exendin-4 compounds
ES2336575T3 (es) 2005-09-22 2010-04-14 Biocompatibles Uk Limited Polipeptidos de fusion glp-1 (peptido-1 similar al glucagon) con resistencia aumentada a la peptidasa.
US8202837B2 (en) * 2005-11-04 2012-06-19 Glaxosmithkline Llc Methods for administering hypoglycemic agents
ATE444741T1 (de) * 2006-05-10 2009-10-15 Biocompatibles Uk Ltd Glp-1 peptide enthaltende kugelförmige mikrokapseln, deren produktion und deren verwendung
PL2038423T3 (pl) * 2006-06-21 2013-05-31 Biocon Ltd Metoda wytwarzania biologicznie aktywnego polipeptydu insulinotropowego
EP2049567A2 (en) 2006-07-18 2009-04-22 Centocor, Inc. Human glp-1 mimetibodies, compositions, methods and uses
EP1972349A1 (en) * 2007-03-21 2008-09-24 Biocompatibles UK Limited GLP-1 fusion peptides conjugated to polymer(s), their production and use
EP1975176A1 (en) * 2007-03-27 2008-10-01 Biocompatibles UK Limited Novel glp-1 fusion peptides, their production and use
CN101220088B (zh) * 2007-09-24 2012-01-25 中国人民解放军第四军医大学 人胰高血糖素样肽-1类似物的融合蛋白及其应用
EP2163243A1 (en) * 2008-09-12 2010-03-17 Biocompatibles UK Limited Treatment of acute myocardial infarction (AMI) using encapsulated cells encoding and secreting GLP-1 peptides or analogs thereof
CN102093475B (zh) * 2009-12-11 2014-02-26 吴晓琰 胰高血糖素样肽-1的类似物
CN102242127B (zh) * 2011-05-20 2012-12-12 严杰 串联抗菌肽Alloferon-1的制备方法及rAlloferon-1-K的应用
SG11201405721VA (en) * 2012-03-19 2014-10-30 Madeleine Pharmaceuticals Ρτy Ltd Method of producing a recombinant peptide
CN107586330B (zh) * 2016-07-08 2021-04-16 上海多米瑞生物技术有限公司 替度鲁肽串联多肽及替度鲁肽制备方法
US20220127324A1 (en) * 2019-02-24 2022-04-28 Oncosimis Biotech Private Limited Method for continuous production of recombinant glp-1 peptide by bacteria
TW202307010A (zh) * 2021-06-30 2023-02-16 美商雅利斯塔製藥公司 單體融合肽及其使用方法

Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995017510A1 (en) * 1993-12-23 1995-06-29 Novo Nordisk A/S A method of producing glucagon-like peptide 1
WO1997029180A1 (en) * 1996-02-06 1997-08-14 Eli Lilly And Company Diabetes therapy
WO1998008873A1 (en) 1996-08-30 1998-03-05 Eli Lilly And Company Use of glucagon-like peptide-1 (glp-1) or analogs to abolish catabolic changes after surgery
WO1999043341A1 (en) 1998-02-27 1999-09-02 Novo Nordisk A/S Glp-1 derivatives with helix-content exceeding 25 %, forming partially structured micellar-like aggregates
WO1999043705A1 (en) 1998-02-27 1999-09-02 Novo Nordisk A/S N-terminally truncated glp-1 derivatives

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5459049A (en) 1989-01-06 1995-10-17 Sanwa Kagaku Kenkyushko Co., Ltd. Motilin-like polypeptide and use thereof
JPH0722517B2 (ja) * 1989-08-24 1995-03-15 株式会社三和化学研究所 モチリン様ポリペプチドの製法並びにそのための組換えｄｎａ及び発現用プラスミド
EP0378078A1 (en) 1989-01-06 1990-07-18 Sanwa Kagaku Kenkyusho Co., Ltd. Motilin-like polypeptide and use thereof
US5545618A (en) 1990-01-24 1996-08-13 Buckley; Douglas I. GLP-1 analogs useful for diabetes treatment
US5595887A (en) * 1990-07-16 1997-01-21 Bionebraska, Inc. Purification directed cloning of peptides using carbonic anhydrase as the affinity binding segment
NZ245015A (en) * 1991-11-05 1995-12-21 Transkaryotic Therapies Inc Delivery of human growth hormone through the administration of transfected cell lines encoding human growth hormone, which are physically protected from host immune response; the transfected cells and their production
JPH06306100A (ja) * 1993-04-26 1994-11-01 Sanwa Kagaku Kenkyusho Co Ltd Ｖｉｐアナログ調製用融合蛋白、ｖｉｐアナログの製法並びにそのための遺伝子組換えプラスミド及び形質転換微生物
US5512459A (en) 1993-07-20 1996-04-30 Bionebraska, Inc. Enzymatic method for modification or recombinant polypeptides
US5912327A (en) * 1996-09-30 1999-06-15 Human Genome Sciences, Inc. Method of purifying chemokines from inclusion bodies
US5846774A (en) * 1997-03-21 1998-12-08 Bionebraska, Inc. Chlorella virus promoters
US6395965B1 (en) * 1997-03-21 2002-05-28 Restoragen, Inc. Plant containing a gene construct comprising a chlorella virus promoter and a lac operator
JPH11178574A (ja) * 1997-12-22 1999-07-06 Toyota Central Res & Dev Lab Inc 新規コラーゲン様タンパク質
ATE424413T1 (de) 2000-06-16 2009-03-15 Lilly Co Eli Analoge des glucagon-ähnlichen peptids-1

Patent Citations (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO1995017510A1 (en) * 1993-12-23 1995-06-29 Novo Nordisk A/S A method of producing glucagon-like peptide 1
WO1997029180A1 (en) * 1996-02-06 1997-08-14 Eli Lilly And Company Diabetes therapy
WO1998008873A1 (en) 1996-08-30 1998-03-05 Eli Lilly And Company Use of glucagon-like peptide-1 (glp-1) or analogs to abolish catabolic changes after surgery
WO1999043341A1 (en) 1998-02-27 1999-09-02 Novo Nordisk A/S Glp-1 derivatives with helix-content exceeding 25 %, forming partially structured micellar-like aggregates
WO1999043705A1 (en) 1998-02-27 1999-09-02 Novo Nordisk A/S N-terminally truncated glp-1 derivatives

Non-Patent Citations (2)

* Cited by examiner, † Cited by third party
Title
HUI H. ET AL.: "Glucagon-like peptide 1 induces differentiation of islet duodenal homeobox-1-positive pancreatic ductal cells into insulin-secreting cells", DIABETES, vol. 50, no. 4, 2001, pages 785 - 96
NIELSEN J.H.. ET AL.: "Regulation of beta-cell mass by hormones and growth factors", DIABETES, vol. 50, no. 1, 2001, pages 25 - 9

Cited By (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7329646B2 (en) 2001-05-10 2008-02-12 Shanghai Huayi Bio-Lab Co., Ltd. Derivatives of the insulinotropic peptide exendin-4 and methods of production thereof
US7176278B2 (en) 2001-08-30 2007-02-13 Biorexis Technology, Inc. Modified transferrin fusion proteins
US8129504B2 (en) 2001-08-30 2012-03-06 Biorexis Technology, Inc. Oral delivery of modified transferrin fusion proteins
EP1626981A4 (en) * 2003-03-04 2006-11-22 Biorexis Pharmaceutical Corp PROTEINS PROTECTED AGAINST DIPEPTIDYLPEPTIDASE
US7867972B2 (en) 2006-07-24 2011-01-11 Pharmacia & Upjohn Company, Llc Fusion protein of exendin-4 to a transferrin (Tf) polypeptide
US8158579B2 (en) 2006-07-24 2012-04-17 Biorexis Pharmaceutical Corporation Fusion protein of an exendin to modified transferrin

Also Published As

Publication number Publication date
KR20040097114A (ko) 2004-11-17
CA2454264C (en) 2010-06-22
ATE443081T1 (de) 2009-10-15
JP4504014B2 (ja) 2010-07-14
EP1408050A1 (en) 2004-04-14
CA2454264A1 (en) 2003-02-27
EP1408050A4 (en) 2005-08-10
ES2333413T3 (es) 2010-02-22
CN1363654A (zh) 2002-08-14
DK1408050T3 (da) 2010-01-18
US7544512B2 (en) 2009-06-09
US20040146985A1 (en) 2004-07-29
AU2002313869B2 (en) 2008-04-03
CN100579986C (zh) 2010-01-13
EP1408050B1 (en) 2009-09-16
JP2005515167A (ja) 2005-05-26
CN1531551A (zh) 2004-09-22
BR0211435A (pt) 2004-07-13
KR100959549B1 (ko) 2010-05-27
BR0211435B1 (pt) 2014-12-23
DE60233734D1 (de) 2009-10-29

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