DE602005026113D1 - Verfahren zur herstellung von plasmid dns in pharmazeutischer qualität - Google Patents

Verfahren zur herstellung von plasmid dns in pharmazeutischer qualität

Info

Publication number
DE602005026113D1
DE602005026113D1 DE602005026113T DE602005026113T DE602005026113D1 DE 602005026113 D1 DE602005026113 D1 DE 602005026113D1 DE 602005026113 T DE602005026113 T DE 602005026113T DE 602005026113 T DE602005026113 T DE 602005026113T DE 602005026113 D1 DE602005026113 D1 DE 602005026113D1
Authority
DE
Germany
Prior art keywords
plasmid dna
order
purification steps
pharmaceutical quality
producing plasmid
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
DE602005026113T
Other languages
English (en)
Inventor
Francis Blanche
Michel Couder
Nicolas Maestrali
Thierry Guillemin
David Gaillac
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Aventis Pharma SA
Original Assignee
Aventis Pharma SA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from PCT/EP2004/011437 external-priority patent/WO2005026331A2/en
Application filed by Aventis Pharma SA filed Critical Aventis Pharma SA
Publication of DE602005026113D1 publication Critical patent/DE602005026113D1/de
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/10—Protozoa; Culture media therefor
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P35/00—Antineoplastic agents
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor
    • C12N15/1006—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers
    • C12N15/101—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor by means of a solid support carrier, e.g. particles, polymers by chromatography, e.g. electrophoresis, ion-exchange, reverse phase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C02—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F—TREATMENT OF WATER, WASTE WATER, SEWAGE, OR SLUDGE
    • C02F2303/00—Specific treatment goals
    • C02F2303/06—Sludge reduction, e.g. by lysis
DE602005026113T 2004-04-19 2005-04-19 Verfahren zur herstellung von plasmid dns in pharmazeutischer qualität Active DE602005026113D1 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US56300804P 2004-04-19 2004-04-19
PCT/EP2004/011437 WO2005026331A2 (en) 2003-09-17 2004-09-17 Method of preparation of pharmaceutically grade plasmid dna
PCT/EP2005/005213 WO2005100542A1 (en) 2004-04-19 2005-04-19 Method for purifying plasmid dna

Publications (1)

Publication Number Publication Date
DE602005026113D1 true DE602005026113D1 (de) 2011-03-10

Family

ID=40635654

Family Applications (1)

Application Number Title Priority Date Filing Date
DE602005026113T Active DE602005026113D1 (de) 2004-04-19 2005-04-19 Verfahren zur herstellung von plasmid dns in pharmazeutischer qualität

Country Status (16)

Country Link
US (1) US20070213289A1 (de)
JP (2) JP2007532123A (de)
KR (1) KR20060135062A (de)
CN (2) CN101006170A (de)
AT (1) ATE496990T1 (de)
AU (2) AU2005233309B2 (de)
BR (1) BRPI0509490A (de)
CA (1) CA2559368A1 (de)
DE (1) DE602005026113D1 (de)
DK (1) DK1737945T3 (de)
EA (1) EA010576B1 (de)
MX (1) MXPA06011568A (de)
NO (1) NO20065290L (de)
NZ (1) NZ549835A (de)
WO (1) WO2005100542A1 (de)
ZA (1) ZA200607622B (de)

Families Citing this family (32)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2559368A1 (en) * 2004-04-19 2005-10-27 Centelion Method for purifying plasmid dna
AU2008256681B2 (en) * 2007-05-23 2014-07-31 Vgxi, Inc Compositions comprising high concentration of biologically active molecules and processes for preparing the same
KR20110028258A (ko) * 2008-04-30 2011-03-17 그래댈리스, 인코포레이티드 고순도 플라스미드 ｄｎａ 제제 및 이의 제조방법
US9149772B2 (en) 2010-01-15 2015-10-06 Board Of Regents, The University Of Texas Systems Enhancing flux of a microporous hollow fiber membrane
US9688921B2 (en) 2013-02-26 2017-06-27 Board Of Regents, The University Of Texas System Oil quality using a microporous hollow fiber membrane
US9643127B2 (en) 2010-01-15 2017-05-09 Board Of Regents Of The University Of Texas System Simultaneous removal of oil and gases from liquid sources using a hollow fiber membrane
US8491792B2 (en) 2010-01-15 2013-07-23 Board Of Regents, The University Of Texas System Non-dispersive process for insoluble oil recovery from aqueous slurries
US8617396B2 (en) 2010-01-15 2013-12-31 Board Of Regents, The University Of Texas System Non-dispersive process for insoluble oil recovery from aqueous slurries
EP2523737A4 (de) * 2010-01-15 2015-10-14 Univ Texas Nichtdispersives verfahren zur gewinnung unlöslicher öle aus wässrigen schlämmen
US9782726B2 (en) 2010-01-15 2017-10-10 Board Of Regents, The University Of Texas System Non-dispersive process for oil recovery
US20130115598A1 (en) * 2011-10-13 2013-05-09 Lawrence Loeb Oligonucleotide probe retrieval assay for dna transactions in mammalian cells
GB2497575A (en) * 2011-12-15 2013-06-19 Protein Technologies Ltd Cell lysis in a flowing liquid using a lytic agent
WO2013188849A1 (en) 2012-06-14 2013-12-19 Board Of Regents, The University Of Texas System Non-dispersive oil recovery from oil industry liquid sources
EP2682168A1 (de) 2012-07-02 2014-01-08 Millipore Corporation Reinigung biologischer Moleküle
CN104370997B (zh) * 2014-09-24 2018-07-31 陈辉 去除生物制品中细菌内毒素的试剂盒、方法及其生物制品的制备方法
CN106884011B (zh) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
WO2017186815A1 (en) * 2016-04-26 2017-11-02 Proqr Therapeutics Ii B.V. Antisense oligonucleotides for enhanced expression of frataxin
AU2017284202B2 (en) * 2016-06-14 2021-11-04 Biogen Ma Inc. Hydrophobic interaction chromatography for purification of oligonucleotides
CN106554956A (zh) * 2016-12-07 2017-04-05 安徽智飞龙科马生物制药有限公司 一种产业化制备BCG‑CpG‑DNA的方法
GB201710130D0 (en) 2017-06-26 2017-08-09 Ge Healthcare Bioprocess R & D Ab A Method of seperation
JP6570795B2 (ja) * 2017-08-25 2019-09-04 積水メディカル株式会社 メチル化ｄｎａ分離及び／又は検出用クロマトグラフィー用充填剤
CN108531563A (zh) * 2018-02-05 2018-09-14 深圳市尚维高科有限公司 多孔微球的用途及裂解液以及裂解液的使用方法
CN110348090B (zh) * 2019-06-28 2021-05-04 浙江大学 基于人工神经网络实现多柱连续流层析设计及分析的方法
CN111088248B (zh) * 2019-12-23 2020-10-16 广州湾区生物科技有限公司 一种用于提取无内毒素质粒的磁性材料及其使用方法
CN111394427B (zh) * 2020-04-22 2023-04-11 宁波市博坤生物科技有限公司 一种含泥土检材中微量dna的提取方法
CN112798697B (zh) * 2020-11-16 2022-11-22 武汉波睿达生物科技有限公司 慢病毒包装系统辅助质粒的超螺旋结构纯度的检测方法
CN115404154A (zh) * 2021-05-10 2022-11-29 艾棣维欣(苏州)生物制品有限公司 用于提取细菌中质粒dna的装置
CN115404232A (zh) * 2021-05-10 2022-11-29 艾棣维欣(苏州)生物制品有限公司 用于提取细菌中质粒dna的方法
CN114085828A (zh) * 2021-11-17 2022-02-25 安徽荃银高科种业股份有限公司 一种水稻基因组dna的快速低成本提取方法及其应用
WO2023095665A1 (ja) * 2021-11-29 2023-06-01 東ソー株式会社 抗体の分離方法
EP4234695A1 (de) * 2022-02-24 2023-08-30 Sartorius BIA Separations d.o.o. Verfahren zur selektiven entfernung von verunreinigungen während eines verfahrens zur reinigung von biologischen mitteln
CN116987581A (zh) * 2023-08-08 2023-11-03 江苏耀海生物制药有限公司 一种质粒dna制备装置及制备工艺

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5561064A (en) * 1994-02-01 1996-10-01 Vical Incorporated Production of pharmaceutical-grade plasmid DNA
FR2728264B1 (fr) * 1994-12-16 1997-01-31 Rhone Poulenc Rorer Sa Purification d'adn par formation de triple helice avec un oligonucleotide immobilise
EP0811055B1 (de) * 1995-12-21 2004-06-09 Genzyme Corporation Methode zur zellyse
FR2746412B1 (fr) * 1996-03-21 1998-06-12 Rhone Poulenc Rorer Sa Purification d'adn plasmidique de qualite pharmaceutique
US7026468B2 (en) * 1996-07-19 2006-04-11 Valentis, Inc. Process and equipment for plasmid purification
US7807822B2 (en) * 1996-08-01 2010-10-05 Robert Bridenbaugh Methods for purifying nucleic acids
FR2773818B1 (fr) * 1998-01-21 2000-02-18 Pasteur Merieux Serums Vacc Procede et dispositif de lyse de bacteries
WO2000073318A1 (en) * 1999-05-28 2000-12-07 Bio Science Contract Production Corporation Methods of dna purification and purified dna
ES2298239T3 (es) * 2000-05-26 2008-05-16 Centelion Purificacion de una formacion de triple helice con oligonucleotido inmovilizado.
EP1320741A4 (de) * 2000-09-18 2006-09-06 Univ Washington Mikrofluidische einrichtungen zur drehmanipulation der fluidischen grenzfläche zwischen mehreren strömungen
US20040002081A1 (en) * 2001-12-18 2004-01-01 Boehringer Ingelheim International Gmbh And Bia Separations D.O.O. Method and device for isolating and purifying a polynucleotide of interest on a manufacturing scale
JP4723254B2 (ja) * 2002-12-23 2011-07-13 バイカル インコーポレイテッド プラスミドｄｎａを精製するための方法
BRPI0413907A (pt) * 2003-09-17 2006-10-24 Centelion método de preparação de plasmìdeo dna de grau farmacêutico
CA2559368A1 (en) * 2004-04-19 2005-10-27 Centelion Method for purifying plasmid dna

Also Published As

Publication number Publication date
MXPA06011568A (es) 2006-12-20
JP2011132249A (ja) 2011-07-07
KR20060135062A (ko) 2006-12-28
CN102031258A (zh) 2011-04-27
WO2005100542A1 (en) 2005-10-27
ZA200607622B (en) 2008-05-28
CN101006170A (zh) 2007-07-25
NO20065290L (no) 2006-11-17
JP2007532123A (ja) 2007-11-15
BRPI0509490A (pt) 2007-09-11
NZ549835A (en) 2008-12-24
AU2005233309A1 (en) 2005-10-27
ATE496990T1 (de) 2011-02-15
CA2559368A1 (en) 2005-10-27
EA200601728A1 (ru) 2007-04-27
AU2005233309B2 (en) 2011-03-31
US20070213289A1 (en) 2007-09-13
AU2011200978A1 (en) 2011-04-07
DK1737945T3 (da) 2011-05-09
EA010576B1 (ru) 2008-10-30

Similar Documents

Publication Publication Date Title
ATE496990T1 (de) Verfahren zur herstellung von plasmid dns in pharmazeutischer qualität
NO20061659L (no) Fremgangsmate for fremstilling av plasmid av hoy farmasoytisk kvalitet
PL356771A1 (en) Magnetic glass particles, method for their preparation and uses thereof
WO2007017229A3 (de) Verfahren für die kontinuierliche zielgerichtete evolution von proteinen in vitro
WO2005059097A3 (en) Methods for high fidelity production of long nucleic acid molecules
WO2003040687A3 (en) Isolation and purification of nucleic acids
WO2007117438A3 (en) Methods for the production of functional protein from dna having a nonsense mutation and the treatment of disorders associated therewith
UA94206C2 (ru) Бактериальные факторы вирулентности и варианты их применения
ES2722204T3 (es) Respuesta inmune mejorada en especies bovinas
WO2020198509A3 (en) Modified oligonucleotides with increased stability
WO2008085962A3 (en) Cell culture methods for producing recombinant proteins in the presence of reduced levels of one or more contaminants
FR2804971B1 (fr) Adn codant une acetohydroxyacide synthase, bacterie le contenant et procede de production de l-valine par culture de cette bacterie
WO2006021453A3 (en) Optimized nucleotide sequences encoding sgp 130
EP2246413A3 (de) Verfahren zur Herstellung einer Plasmid-DNA in pharmazeutischer Qualität
Pereira et al. New approach for purification of pre-miR-29 using lysine-affinity chromatography
ATE371731T1 (de) Verfahren zur skalierbaren reinigung von plasmid- dns
EA200700415A1 (ru) Улучшенные штаммы e. coly для продуцирования плазмидной днк
WO2004081166A3 (de) Nukleotidsequenzen coryneformer bakterien codierend für am l-serinstoffwechsel beteiligte proteine sowie verfahren zur mikrobiellen herstellung von l-serin
WO2006055040A3 (en) Identification of proteins in a genome
ATE451457T1 (de) Verfahren zur dipeptidherstellung
ATE553194T1 (de) Verfahren zur dipeptidherstellung
ATE332971T1 (de) Typ ii dns polymerase aus pyrococcus abyssi
WO2006081529A3 (en) Compositions and processes for improved plasmid dna production
WO2005072092A3 (en) Systems for tightly regulated gene expression
DE502006002664D1 (de) Verfahren zur herstellung von lipasen