BRPI0413907A - método de preparação de plasmìdeo dna de grau farmacêutico - Google Patents

método de preparação de plasmìdeo dna de grau farmacêutico

Info

Publication number
BRPI0413907A
BRPI0413907A BRPI0413907-0A BRPI0413907A BRPI0413907A BR PI0413907 A BRPI0413907 A BR PI0413907A BR PI0413907 A BRPI0413907 A BR PI0413907A BR PI0413907 A BRPI0413907 A BR PI0413907A
Authority
BR
Brazil
Prior art keywords
pharmaceutical grade
dna plasmid
plasmid preparation
order
purification steps
Prior art date
Application number
BRPI0413907-0A
Other languages
English (en)
Inventor
Francis Blanches
Michel Coudert
Nicolas Maestrali
David Gaillac
Thierry Guillemin
Original Assignee
Centelion
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Centelion filed Critical Centelion
Publication of BRPI0413907A publication Critical patent/BRPI0413907A/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K48/00—Medicinal preparations containing genetic material which is inserted into cells of the living body to treat genetic diseases; Gene therapy
    • A61K48/0091—Purification or manufacturing processes for gene therapy compositions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M47/00—Means for after-treatment of the produced biomass or of the fermentation or metabolic products, e.g. storage of biomass
    • C12M47/06—Hydrolysis; Cell lysis; Extraction of intracellular or cell wall material
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/06—Lysis of microorganisms
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1003—Extracting or separating nucleic acids from biological samples, e.g. pure separation or isolation methods; Conditions, buffers or apparatuses therefor

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Mycology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Sustainable Development (AREA)
  • Analytical Chemistry (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Manufacturing & Machinery (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Saccharide Compounds (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Medicinal Preparation (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

"MéTODO DE PREPARAçãO DE PLASMìDEO DNA DE GRAU FARMACêUTICO". A invenção proporciona um processo para lise alcalina contínua de uma suspensão bacterial de modo a recolher pDNA. Ela também proporciona etapas de purificação adicional opcional, incluindo filtração de lisato, uma cromatografia de troca de ânion, cromatografia de afinidade triplex, e cromatografia de interação hidrofóbica. Estas etapas de purificação adicionais podem ser combinadas com a lise contínua de modo a produzir um produto de pDNA altamente purificado substancialmente livre de gDNA, RNA, proteína, endotoxina, e outros contaminantes.
BRPI0413907-0A 2003-09-17 2004-09-17 método de preparação de plasmìdeo dna de grau farmacêutico BRPI0413907A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US50346403P 2003-09-17 2003-09-17
US56300804P 2004-04-19 2004-04-19
PCT/EP2004/011437 WO2005026331A2 (en) 2003-09-17 2004-09-17 Method of preparation of pharmaceutically grade plasmid dna

Publications (1)

Publication Number Publication Date
BRPI0413907A true BRPI0413907A (pt) 2006-10-24

Family

ID=42827706

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0413907-0A BRPI0413907A (pt) 2003-09-17 2004-09-17 método de preparação de plasmìdeo dna de grau farmacêutico

Country Status (18)

Country Link
US (1) US20070111221A1 (pt)
EP (1) EP1664277B1 (pt)
JP (1) JP2007505613A (pt)
KR (1) KR101094032B1 (pt)
CN (1) CN1882682B (pt)
AT (1) ATE532853T1 (pt)
AU (2) AU2004272748C1 (pt)
BR (1) BRPI0413907A (pt)
CA (1) CA2536931A1 (pt)
EA (1) EA009447B1 (pt)
ES (1) ES2376173T3 (pt)
HK (1) HK1096994A1 (pt)
IL (1) IL174147A0 (pt)
MX (1) MXPA06003004A (pt)
NO (1) NO20061659L (pt)
NZ (1) NZ545449A (pt)
PT (1) PT1664277E (pt)
WO (1) WO2005026331A2 (pt)

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EP2054498A1 (de) * 2006-08-15 2009-05-06 Basf Se Verfahren zur isolierung von proteinen aus produktionszellen
CA2668818C (en) * 2006-10-10 2018-06-26 Xenomics, Inc. Compositions, methods and kits for isolating nucleic acids from body fluids using anion exchange media
KR101523111B1 (ko) 2007-02-28 2015-06-25 리폭센 테크놀로지즈 리미티드 폴리시알산의 내독소 감소
WO2009025690A2 (en) 2007-05-23 2009-02-26 Nature Technology Corporation Improved e. coli plasmid dna production
EP2153260B1 (en) * 2007-05-23 2017-01-25 VGXI, Inc. Composition comprising high concentration of biologically active molecules and processes for preparing the same
WO2008150838A1 (en) * 2007-05-31 2008-12-11 Ge Healthcare Uk Limited Improved miniprep system for simple and rapid plasmid dna extraction
ES2556711T3 (es) * 2008-04-09 2016-01-19 Viromed Co., Ltd. Formulaciones liofilizadas de ADN para el aumento de la expresión del ADN plasmídico
WO2011057199A1 (en) * 2009-11-06 2011-05-12 Adenios, Inc. Compositions for treating cns disorders
RU2488406C1 (ru) * 2012-02-16 2013-07-27 Общество с ограниченной ответственностью "НТфарма" Фармацевтическая композиция для терапии острых токсических состояний
RU2489168C1 (ru) * 2012-02-28 2013-08-10 Общество с ограниченной ответственностью "НТфарма" Фармацевтическая композиция, продуцирующая антиоксидантный, антимикробный, антитоксический белок - лактоферрин человека, способ ее получения и способ терапии
EP2682168A1 (en) 2012-07-02 2014-01-08 Millipore Corporation Purification of biological molecules
CN104099321A (zh) * 2014-07-10 2014-10-15 广州市军科泰特医药科技有限公司 抗肺癌T-VISA-BikDD质粒的制备方法
US20180036409A1 (en) * 2014-12-29 2018-02-08 Bonac Corporation Composition containing nucleic acid molecule stably
CN106884011B (zh) * 2015-12-16 2020-11-17 固安鼎泰海规生物科技有限公司 一种规模化质粒纯化的联合液相层析分离方法
US11441110B2 (en) 2016-05-06 2022-09-13 MAX-PLANCK-Gesellschaft zur Förderung der Wissenschaften e.V. Plug flow tubular bioreactor, system containing the same and method for production of virus
EP3802781A4 (en) * 2018-05-31 2022-03-30 VGXI, Inc. LYTIC COIL AND ITS USES FOR THE ISOLATION AND PURIFICATION OF POLYNUCLEOTIDS
IL296722A (en) 2020-03-31 2022-11-01 Richter Helm Biologics Gmbh & Co Kg Methods for the production of plasmid DNA
CN111394427B (zh) * 2020-04-22 2023-04-11 宁波市博坤生物科技有限公司 一种含泥土检材中微量dna的提取方法
EP3906982B1 (de) 2020-05-08 2022-03-16 AXAGARIUS GmbH & Co. KG Verfahren zur plasmidreinigung unter gleichzeitiger abreicherung von endotoxinen
JP2021074018A (ja) * 2021-02-12 2021-05-20 マックス−プランク−ゲゼルシャフト・ツア・フェルデルング・デア・ヴィッセンシャフテン・エー・ファオ プラグフローチューブ型バイオリアクター、それを含むシステム、およびウイルスの調製のための方法
CN115404232A (zh) * 2021-05-10 2022-11-29 艾棣维欣(苏州)生物制品有限公司 用于提取细菌中质粒dna的方法
CN113332869B (zh) * 2021-06-23 2022-12-23 苏州沃美生物有限公司 混合装置和方法以及重组大肠杆菌质粒提取方法
CN117187069B (zh) * 2023-09-18 2024-04-05 苏州依科赛生物科技股份有限公司 一种碱裂解工艺的大肠杆菌hcp检测方法

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Also Published As

Publication number Publication date
PT1664277E (pt) 2012-01-17
AU2004272748B2 (en) 2009-12-03
AU2010200801A1 (en) 2010-03-25
KR20060086941A (ko) 2006-08-01
AU2004272748A1 (en) 2005-03-24
CN1882682A (zh) 2006-12-20
WO2005026331A3 (en) 2005-11-17
EP1664277B1 (en) 2011-11-09
IL174147A0 (en) 2006-08-01
US20070111221A1 (en) 2007-05-17
KR101094032B1 (ko) 2011-12-19
CN1882682B (zh) 2010-06-16
EA200600579A1 (ru) 2006-10-27
CA2536931A1 (en) 2005-03-24
JP2007505613A (ja) 2007-03-15
ES2376173T3 (es) 2012-03-09
HK1096994A1 (en) 2007-06-15
AU2004272748C1 (en) 2010-07-08
EP1664277A2 (en) 2006-06-07
NO20061659L (no) 2006-06-13
NZ545449A (en) 2009-07-31
ATE532853T1 (de) 2011-11-15
WO2005026331A2 (en) 2005-03-24
MXPA06003004A (es) 2006-06-23
EA009447B1 (ru) 2007-12-28

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]