MX2022005685A - Uso de proteina cas9 de la bacteria pasteurella pneumotropica. - Google Patents

Uso de proteina cas9 de la bacteria pasteurella pneumotropica.

Info

Publication number
MX2022005685A
MX2022005685A MX2022005685A MX2022005685A MX2022005685A MX 2022005685 A MX2022005685 A MX 2022005685A MX 2022005685 A MX2022005685 A MX 2022005685A MX 2022005685 A MX2022005685 A MX 2022005685A MX 2022005685 A MX2022005685 A MX 2022005685A
Authority
MX
Mexico
Prior art keywords
nuclease
cas9
crispr
bacterium
cas9 protein
Prior art date
Application number
MX2022005685A
Other languages
English (en)
Inventor
Konstantin Viktorovich Severinov
Polina Anatolevna Selkova
Daria Nikolaevna Artamonova
Olga Sergeevna Musharova
Tatiana Olegovna Artamonova
Marina Viktorovna Abramova
Sergey Anatolievich Shmakov
Ignaty Igorevich Goryanin
Julia Valerevna Andreeva
Tatiana Igorevna Zyubko
Mikhail Alekseevich Khodorkovskii
George Evgenevich Pobegalov
Anatoliy Nikolaevich Arseniev
Aleksandra Andreevna Vasilieva
Iana Vitalievna Fedorova
Original Assignee
Biocad Joint Stock Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Biocad Joint Stock Co filed Critical Biocad Joint Stock Co
Publication of MX2022005685A publication Critical patent/MX2022005685A/es

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C12N9/22—Ribonucleases RNAses, DNAses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/102—Mutagenizing nucleic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2310/00—Structure or type of the nucleic acid
    • C12N2310/10—Type of nucleic acid
    • C12N2310/20—Type of nucleic acid involving clustered regularly interspaced short palindromic repeats [CRISPRs]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y301/00—Hydrolases acting on ester bonds (3.1)

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

La presente invención describe una novedosa nucleasa bacteriana del sistema CRISPR-Cas9 de la bacteria P. pneumotropica, así como también su uso para producir rupturas de cadena doble estrictamente específicas en una molécula de ADN. Esta nucleasa tiene propiedades inusuales y se puede utilizar como herramienta de modificación de la secuencia de ADN genómico en la célula de un organismo unicelular o multicelular. Así, aumenta la versatilidad de los sistemas CRISPR-Cas9 disponibles, hecho que permite el uso de distintas variantes de nucleasas Cas9 para el corte de ADN genómico o plásmido de distintos organismos en un número mayor de sitios específicos y/o en condiciones específicas.
MX2022005685A 2019-11-11 2020-07-02 Uso de proteina cas9 de la bacteria pasteurella pneumotropica. MX2022005685A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
RU2019136164A RU2724470C1 (ru) 2019-11-11 2019-11-11 Применение cas9 белка из бактерии pasteurella pneumotropica для модификации геномной днк в клетках
PCT/RU2020/050145 WO2021096391A1 (ru) 2019-11-11 2020-07-02 Применение cas9 бежа из бактерии pasteurella pneumotropica

Publications (1)

Publication Number Publication Date
MX2022005685A true MX2022005685A (es) 2022-07-27

Family

ID=71136150

Family Applications (1)

Application Number Title Priority Date Filing Date
MX2022005685A MX2022005685A (es) 2019-11-11 2020-07-02 Uso de proteina cas9 de la bacteria pasteurella pneumotropica.

Country Status (16)

Country Link
US (1) US20220403369A1 (es)
EP (1) EP4056705A4 (es)
JP (1) JP2023501524A (es)
KR (1) KR20220145324A (es)
CN (1) CN115397995A (es)
AU (1) AU2020384851A1 (es)
BR (1) BR112022009148A2 (es)
CA (1) CA3157898A1 (es)
CL (1) CL2022001220A1 (es)
CO (1) CO2022006156A2 (es)
MA (1) MA57032A1 (es)
MX (1) MX2022005685A (es)
PE (1) PE20230035A1 (es)
RU (1) RU2724470C1 (es)
WO (1) WO2021096391A1 (es)
ZA (1) ZA202205208B (es)

Family Cites Families (2)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20220010293A1 (en) * 2018-02-23 2022-01-13 Pioneer Hi-Bred International, Inc. Novel cas9 orthologs
BR112022017713A2 (pt) * 2020-03-04 2022-11-16 Flagship Pioneering Innovations Vi Llc Métodos e composições para modular um genoma

Also Published As

Publication number Publication date
JP2023501524A (ja) 2023-01-18
US20220403369A1 (en) 2022-12-22
ZA202205208B (en) 2023-04-26
BR112022009148A2 (pt) 2023-05-02
CN115397995A (zh) 2022-11-25
KR20220145324A (ko) 2022-10-28
RU2724470C1 (ru) 2020-06-23
CA3157898A1 (en) 2021-05-20
CO2022006156A2 (es) 2023-01-26
EP4056705A4 (en) 2023-12-27
MA57032A1 (fr) 2023-01-31
AU2020384851A1 (en) 2022-12-01
EP4056705A1 (en) 2022-09-14
PE20230035A1 (es) 2023-01-10
CL2022001220A1 (es) 2023-01-06
WO2021096391A1 (ru) 2021-05-20

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MX2022005685A (es) Uso de proteina cas9 de la bacteria pasteurella pneumotropica.