DE60036641D1 - Mutanter apre promoter - Google Patents

Mutanter apre promoter

Info

Publication number
DE60036641D1
DE60036641D1 DE60036641T DE60036641T DE60036641D1 DE 60036641 D1 DE60036641 D1 DE 60036641D1 DE 60036641 T DE60036641 T DE 60036641T DE 60036641 T DE60036641 T DE 60036641T DE 60036641 D1 DE60036641 D1 DE 60036641D1
Authority
DE
Germany
Prior art keywords
mutant
apre promoter
promoter
mutant apre
apre
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60036641T
Other languages
English (en)
Other versions
DE60036641T2 (de
Inventor
Eugenio Ferrari
Fernando Valle
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Danisco US Inc
Original Assignee
Genencor International Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor International Inc filed Critical Genencor International Inc
Publication of DE60036641D1 publication Critical patent/DE60036641D1/de
Application granted granted Critical
Publication of DE60036641T2 publication Critical patent/DE60036641T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
DE60036641T 2000-01-07 2000-12-21 Mutanter apre promoter Expired - Lifetime DE60036641T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US479494 2000-01-07
US09/479,494 US6509185B1 (en) 2000-01-07 2000-01-07 Mutant aprE promotor
PCT/US2000/035312 WO2001051643A1 (en) 2000-01-07 2000-12-21 Mutant apre promoter

Publications (2)

Publication Number Publication Date
DE60036641D1 true DE60036641D1 (de) 2007-11-15
DE60036641T2 DE60036641T2 (de) 2008-07-03

Family

ID=23904251

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60036641T Expired - Lifetime DE60036641T2 (de) 2000-01-07 2000-12-21 Mutanter apre promoter

Country Status (12)

Country Link
US (2) US6509185B1 (de)
EP (1) EP1244794B1 (de)
JP (1) JP4870306B2 (de)
KR (1) KR20020073497A (de)
CN (2) CN101054588A (de)
AT (1) ATE374822T1 (de)
AU (1) AU2739001A (de)
CA (1) CA2396250C (de)
DE (1) DE60036641T2 (de)
DK (1) DK1244794T3 (de)
MX (1) MXPA02006614A (de)
WO (1) WO2001051643A1 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
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US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
DE60335703D1 (de) * 2002-02-15 2011-02-24 Danisco Us Inc Verbesserte proteinexpression in bacillus subtilis
AU2003228393A1 (en) * 2002-03-29 2003-10-13 Genencor International, Inc. Ehanced protein expression in bacillus
EP2460823B1 (de) 2007-05-10 2014-02-26 Danisco US Inc. Modifiziertes Ausscheidungssystem zur Erhöhung der Expression von Polypeptiden in Bakterien
US20090048136A1 (en) * 2007-08-15 2009-02-19 Mcdonald Hugh C Kappa-carrageenase and kappa-carrageenase-containing compositions
US8044192B2 (en) 2007-09-12 2011-10-25 Danisco Us Inc. Trichoderma promoter
RU2515112C2 (ru) 2007-12-21 2014-05-10 ДАНИСКО ЮЭс ИНК. Улучшенная продукция белка в bacillus
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
JP6378089B2 (ja) 2011-12-09 2018-08-22 ダニスコ・ユーエス・インク 微生物におけるタンパク質産生のための、Ｂ．ズブチリス（Ｂ．ｓｕｂｔｉｌｉｓ）からのリボソームプロモーター
JP7012533B2 (ja) * 2014-12-16 2022-02-14 ダニスコ・ユーエス・インク タンパク質発現の増強
US11306319B2 (en) 2015-10-30 2022-04-19 Danisco Us Inc. Enhanced protein expression and methods thereof
CN108779154B (zh) 2015-12-23 2022-12-27 丹尼斯科美国公司 增强的蛋白质产生及其方法
JP7103948B2 (ja) 2016-03-04 2022-07-20 ダニスコ・ユーエス・インク 微生物におけるタンパク質産生のための遺伝子操作されたリボソームプロモーター
EP3558026A1 (de) 2016-12-21 2019-10-30 DuPont Nutrition Biosciences ApS Verfahren zur verwendung von thermostabilen serinproteasen
DK3571217T3 (da) 2017-01-23 2023-09-18 Danisco Us Inc Fremgangsmåder og sammensætninger til opnåelse af naturlig kompetence i bacillus-værtsceller
KR102375732B1 (ko) 2017-02-24 2022-03-16 다니스코 유에스 인크. 바실러스 리체니포르미스에서 단백질 생산을 증가시키기 위한 조성물 및 방법
EP3583210B1 (de) 2017-03-15 2021-07-07 Danisco US Inc. Trypsinähnliche serinproteasen und deren verwendungen
BR112019018969A2 (pt) 2017-03-15 2020-04-22 Dupont Nutrition Biosci Aps serina proteases similar à tripsina e usos das mesmas
RU2019132448A (ru) 2017-03-15 2021-04-15 ДюПон НЬЮТРИШН БАЙОСАЙЕНСИЗ АпС Способы применения сериновой протеазы архей
EP3607097B1 (de) 2017-04-07 2023-06-21 DuPont Nutrition Biosciences ApS Bacillus-wirtszellen, welche beta-galactosidasen und lactasen in abwesenheit von p-nitrobenzylesterase nebenaktivität produzieren
CN107142259A (zh) * 2017-05-25 2017-09-08 中国科学院上海高等研究院 一种表达外源基因的启动子及其应用
EP3655537A1 (de) 2017-08-23 2020-05-27 Danisco US Inc. Verfahren und zusammensetzungen zur effizienten genetischen modifikation von bacillus-licheniformis-stämmen
EP3682010A1 (de) 2017-09-13 2020-07-22 Danisco US Inc. Modifizierte sequenzen von 5'-untranslatierten regionen (utr) für erhöhte proteinproduktion in bacillus
CN108118058B (zh) * 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其应用
CN108118059B (zh) * 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
CN111670244B (zh) 2018-01-03 2023-08-01 丹尼斯科美国公司 突变的和遗传修饰的芽孢杆菌属细胞及其用于增加蛋白质产生的方法
CN112654703A (zh) 2018-07-06 2021-04-13 杜邦营养生物科学有限公司 用于基于谷类的动物饲料的含木聚糖酶饲料添加剂
EP3887526A1 (de) * 2018-11-28 2021-10-06 Danisco US Inc. Neuartige promotorsequenzen und verfahren dafür zur verbesserten proteinproduktion in bacillus-zellen
CN114096676A (zh) 2019-02-20 2022-02-25 巴斯夫欧洲公司 用确定成分培养基和镁补料的芽孢杆菌工业发酵工艺
WO2020169564A1 (en) 2019-02-20 2020-08-27 Basf Se Industrial fermentation process for bacillus using defined medium and trace element feed
US20220177923A1 (en) 2019-04-05 2022-06-09 Danisco Us Inc Methods for integrating a donor DNA sequence into the genome of bacillus using linear recombinant DNA constructs and compositions thereof
CA3136113A1 (en) 2019-04-05 2020-10-08 Danisco Us Inc. Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof
WO2021030267A1 (en) 2019-08-14 2021-02-18 Danisco Us Inc Compositions and methods for increased protein production in bacillus licheniformis
EP4051793A1 (de) 2019-10-28 2022-09-07 Danisco US Inc Mikrobielle wirtszellen zur herstellung von heterologen cyanursäurehydrolasen und biurethydrolasen
JP2023500362A (ja) 2019-11-11 2023-01-05 ダニスコ・ユーエス・インク バチルス属（ｂａｃｉｌｌｕｓ）細胞における強化されたタンパク質産生のための組成物及び方法
KR20220098245A (ko) 2019-11-19 2022-07-11 다니스코 유에스 인크. 바실러스 게놈 및 이의 조성물을 변형시키기 위한 선택 마커-부재 방법
JP2023524334A (ja) 2020-01-15 2023-06-12 ダニスコ・ユーエス・インク バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における強化したタンパク質産生のための組成物及び方法
WO2021224152A1 (en) 2020-05-05 2021-11-11 Basf Se Improving expression in fermentation processes
CN113755492B (zh) * 2020-07-20 2023-05-30 中国科学院天津工业生物技术研究所 丙酮酸羧化酶基因启动子的突变体及其应用
EP4294823A1 (de) 2021-02-22 2023-12-27 Danisco US Inc. Verfahren und zusammensetzungen zur herstellung von proteinen von interesse in pigmentdefizienten bacillus-zellen
EP4347812A1 (de) 2021-05-24 2024-04-10 Danisco US Inc. Zusammensetzungen und verfahren zur verbesserten proteinproduktion in bacillus-zellen
CN117813317A (zh) 2021-06-24 2024-04-02 巴斯夫欧洲公司 改良的芽孢杆菌生产宿主
WO2022269084A1 (en) 2021-06-24 2022-12-29 Basf Se Improved bacillus host cell with altered rema/remb protein
WO2023091878A1 (en) 2021-11-16 2023-05-25 Danisco Us Inc. Compositions and methods for enhanced protein production in bacillus cells
WO2023233996A1 (ja) * 2022-05-31 2023-12-07 パナソニックＩｐマネジメント株式会社 改変プロモーター、発現ベクター、微生物、物質の生産方法、改変シアノバクテリア及び改変プロモーターの作製方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
DK87092D0 (da) * 1992-07-02 1992-07-02 Novo Nordisk As Nyt enzym
US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor

Also Published As

Publication number Publication date
EP1244794B1 (de) 2007-10-03
JP2003519492A (ja) 2003-06-24
CN1425071A (zh) 2003-06-18
CA2396250C (en) 2009-02-24
CN1313615C (zh) 2007-05-02
DE60036641T2 (de) 2008-07-03
ATE374822T1 (de) 2007-10-15
CA2396250A1 (en) 2001-07-19
MXPA02006614A (es) 2004-09-10
US6911322B2 (en) 2005-06-28
JP4870306B2 (ja) 2012-02-08
DK1244794T3 (da) 2008-01-28
US6509185B1 (en) 2003-01-21
EP1244794A1 (de) 2002-10-02
US20030148461A1 (en) 2003-08-07
WO2001051643A1 (en) 2001-07-19
AU2739001A (en) 2001-07-24
KR20020073497A (ko) 2002-09-26
CN101054588A (zh) 2007-10-17

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Legal Events

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