ATE374822T1 - Mutanter apre promoter - Google Patents

Mutanter apre promoter

Info

Publication number
ATE374822T1
ATE374822T1 AT00990354T AT00990354T ATE374822T1 AT E374822 T1 ATE374822 T1 AT E374822T1 AT 00990354 T AT00990354 T AT 00990354T AT 00990354 T AT00990354 T AT 00990354T AT E374822 T1 ATE374822 T1 AT E374822T1
Authority
AT
Austria
Prior art keywords
mutant
apre promoter
promoter
mutant apre
apre
Prior art date
Application number
AT00990354T
Other languages
English (en)
Inventor
Eugenio Ferrari
Fernando Valle
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Application granted granted Critical
Publication of ATE374822T1 publication Critical patent/ATE374822T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus
AT00990354T 2000-01-07 2000-12-21 Mutanter apre promoter ATE374822T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US09/479,494 US6509185B1 (en) 2000-01-07 2000-01-07 Mutant aprE promotor

Publications (1)

Publication Number Publication Date
ATE374822T1 true ATE374822T1 (de) 2007-10-15

Family

ID=23904251

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00990354T ATE374822T1 (de) 2000-01-07 2000-12-21 Mutanter apre promoter

Country Status (12)

Country Link
US (2) US6509185B1 (de)
EP (1) EP1244794B1 (de)
JP (1) JP4870306B2 (de)
KR (1) KR20020073497A (de)
CN (2) CN101054588A (de)
AT (1) ATE374822T1 (de)
AU (1) AU2739001A (de)
CA (1) CA2396250C (de)
DE (1) DE60036641T2 (de)
DK (1) DK1244794T3 (de)
MX (1) MXPA02006614A (de)
WO (1) WO2001051643A1 (de)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
ATE495245T1 (de) * 2002-02-15 2011-01-15 Danisco Us Inc Verbesserte proteinexpression in bacillus subtilis
EP2339017A3 (de) 2002-03-29 2011-10-12 Genencor International, Inc. Erhöhte Proteinexpression in Bacillus
ATE550348T1 (de) 2007-05-10 2012-04-15 Danisco Us Inc Modifiziertes ausscheidungssystem zur erhöhung der expression von polypeptiden in bakterien
US20090048136A1 (en) * 2007-08-15 2009-02-19 Mcdonald Hugh C Kappa-carrageenase and kappa-carrageenase-containing compositions
US8044192B2 (en) 2007-09-12 2011-10-25 Danisco Us Inc. Trichoderma promoter
KR20100098652A (ko) 2007-12-21 2010-09-08 다니스코 유에스 인크. 바실루스 내에서의 향상된 단백질 생산
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
DK2788491T3 (en) 2011-12-09 2019-04-01 Danisco Us Inc RIBOSOMAL PROMOTERS FROM B. SUBTILIS FOR PROTEIN PRODUCTION IN MICRO-ORGANISMS
US10683528B2 (en) * 2014-12-16 2020-06-16 Danisco Us Inc Enhanced protein expression
JP7025325B2 (ja) 2015-10-30 2022-02-24 ダニスコ・ユーエス・インク タンパク質発現の増強およびその方法
WO2017112733A1 (en) 2015-12-23 2017-06-29 Danisco Us Inc. Enhanced protein production and methods thereof
DK3419991T3 (da) 2016-03-04 2023-01-16 Danisco Us Inc Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer
CN110381746B (zh) 2016-12-21 2023-12-01 杜邦营养生物科学有限公司 使用热稳定丝氨酸蛋白酶的方法
EP3571217B1 (de) 2017-01-23 2023-06-21 Danisco US Inc. Verfahren und zusammensetzungen zur gewinnung von natürlicher kompetenz in bacillus-wirtszellen
US11866713B2 (en) 2017-02-24 2024-01-09 Danisco Us Inc. Compositions and methods for increased protein production in bacillus licheniformis
BR112019018969A2 (pt) 2017-03-15 2020-04-22 Dupont Nutrition Biosci Aps serina proteases similar à tripsina e usos das mesmas
WO2018169780A1 (en) 2017-03-15 2018-09-20 Dupont Nutrition Biosciences Aps Methods of using an archaeal serine protease
WO2018169750A1 (en) 2017-03-15 2018-09-20 Danisco Us Inc Trypsin-like serine proteases and uses thereof
DK3607097T3 (da) 2017-04-07 2023-09-18 Dupont Nutrition Biosci Aps Bacillus-værtsceller, der danner beta-galactosidaser og lactaser i fravær af p-nitrobenzylesterase-sideaktivitet
CN107142259A (zh) * 2017-05-25 2017-09-08 中国科学院上海高等研究院 一种表达外源基因的启动子及其应用
EP3655537A1 (de) 2017-08-23 2020-05-27 Danisco US Inc. Verfahren und zusammensetzungen zur effizienten genetischen modifikation von bacillus-licheniformis-stämmen
CN111094576A (zh) 2017-09-13 2020-05-01 丹尼斯科美国公司 用于增加芽胞杆菌属中蛋白质产生的经修饰的5′-非翻译区(utr)序列
CN108118058B (zh) * 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其应用
CN108118059B (zh) * 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
KR20200105879A (ko) 2018-01-03 2020-09-09 다니스코 유에스 인크. 증가된 단백질 생산을 위한 돌연변이체 및 유전자 변형된 바실러스 세포 및 이의 방법
US20210277374A1 (en) 2018-07-06 2021-09-09 Dupont Nutrition Biosciences Aps Xylanase-containing feed additives for cereal-based animal feed
US11781147B2 (en) * 2018-11-28 2023-10-10 Danisco Us Inc. Promoter sequences and methods thereof for enhanced protein production in Bacillus cells
US20220186177A1 (en) 2019-02-20 2022-06-16 Basf Se Industrial fermentation process for bacillus using defined medium and magnesium feed
MX2021010109A (es) 2019-02-20 2021-09-21 Basf Se Proceso de fermentacion industrial para bacillus mediante el uso de un medio definido y una alimentacion de oligoelementos.
US20220162621A1 (en) 2019-04-05 2022-05-26 Danisco Us Inc Methods For Polynucleotide Integration Into The Genome Of Bacillus Using Dual Circular Recombinant DNA Constructs And Compositions Thereof
JP2022526982A (ja) 2019-04-05 2022-05-27 ダニスコ・ユーエス・インク 線状組換えＤＮＡコンストラクトを使用してバチルス（Ｂａｃｉｌｌｕｓ）のゲノムにドナーＤＮＡ配列を組み込むための方法及びその組成物
EP4031560A1 (de) 2019-08-14 2022-07-27 Danisco US Inc Zusammensetzungen und verfahren zur erhöhung der proteinproduktion in bacillus licheniformis
EP4051793A1 (de) 2019-10-28 2022-09-07 Danisco US Inc Mikrobielle wirtszellen zur herstellung von heterologen cyanursäurehydrolasen und biurethydrolasen
CN115335503A (zh) 2019-11-11 2022-11-11 丹尼斯科美国公司 用于增强芽孢杆菌属细胞中蛋白质产生的组合物和方法
KR20220098245A (ko) 2019-11-19 2022-07-11 다니스코 유에스 인크. 바실러스 게놈 및 이의 조성물을 변형시키기 위한 선택 마커-부재 방법
JP2023524334A (ja) 2020-01-15 2023-06-12 ダニスコ・ユーエス・インク バチルス・リケニフォルミス（ｂａｃｉｌｌｕｓ ｌｉｃｈｅｎｉｆｏｒｍｉｓ）における強化したタンパク質産生のための組成物及び方法
WO2021224152A1 (en) 2020-05-05 2021-11-11 Basf Se Improving expression in fermentation processes
CN113755492B (zh) * 2020-07-20 2023-05-30 中国科学院天津工业生物技术研究所 丙酮酸羧化酶基因启动子的突变体及其应用
US20240101611A1 (en) 2021-02-22 2024-03-28 Danisco Us Inc. Methods and compositions for producing proteins of interest in pigment deficient bacillus cells
CN117769597A (zh) 2021-05-24 2024-03-26 丹尼斯科美国公司 用于增强芽孢杆菌属细胞中蛋白质产生的组合物和方法
EP4359424A1 (de) 2021-06-24 2024-05-01 Basf Se Verbesserter bacillus-produktions-host
BR112023027016A2 (pt) 2021-06-24 2024-03-12 Basf Se Célula hospedeira modificada de bacillus compreendendo uma proteína reguladora da matriz extracelular a alterada e/ou uma proteína reguladora da matriz extracelular b alterada, método para produzir um composto de interesse, e, proteínas reguladoras das matrizes extracelulares a e b alteradas
WO2023091878A1 (en) 2021-11-16 2023-05-25 Danisco Us Inc. Compositions and methods for enhanced protein production in bacillus cells
WO2023233996A1 (ja) * 2022-05-31 2023-12-07 パナソニックＩｐマネジメント株式会社 改変プロモーター、発現ベクター、微生物、物質の生産方法、改変シアノバクテリア及び改変プロモーターの作製方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
DK87092D0 (da) * 1992-07-02 1992-07-02 Novo Nordisk As Nyt enzym
US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor

Also Published As

Publication number Publication date
JP2003519492A (ja) 2003-06-24
EP1244794A1 (de) 2002-10-02
WO2001051643A1 (en) 2001-07-19
CN1313615C (zh) 2007-05-02
AU2739001A (en) 2001-07-24
MXPA02006614A (es) 2004-09-10
CA2396250A1 (en) 2001-07-19
US20030148461A1 (en) 2003-08-07
DE60036641D1 (de) 2007-11-15
JP4870306B2 (ja) 2012-02-08
DK1244794T3 (da) 2008-01-28
US6911322B2 (en) 2005-06-28
DE60036641T2 (de) 2008-07-03
CN1425071A (zh) 2003-06-18
KR20020073497A (ko) 2002-09-26
CN101054588A (zh) 2007-10-17
EP1244794B1 (de) 2007-10-03
US6509185B1 (en) 2003-01-21
CA2396250C (en) 2009-02-24

Similar Documents

Publication Publication Date Title
DE60036641D1 (de) Mutanter apre promoter
YU9404A (sh) Multimerna, usmerena kontrastna sredstva na bazi peptida
EP1553975B8 (de) Optimierte fc-varianten und herstellungsverfahren dafür
DK1456366T3 (da) Ny alkalisk protease fra Bacillus gibsonii (DSM 14393) og vaske- og ren-gøringsmidler indeholdende denne nye alkaliske protease
AU2002361402A1 (en) Novel alkaline protease from bacillus sp. (dsm 14392) and washing and cleaning products comprising said novel alkaline protease
SG158141A1 (en) Pancreatic polypeptide family motifs, polypeptides and methods comprising the same
NO20020348D0 (no) Peptid acceptor ligeringsmetoder
AU2003299585A1 (en) Water-soluble products and methods of making and using the same
MY113489A (en) Reversible protease inhibitors
DE60120712D1 (en) Kallikrein gen
DK1530637T3 (da) Carlsberg-subtilisin-proteiner med nedsat immunogenitet
AU2002354696A1 (en) Method for purifying an enzyme, a purified enzyme produced thereby, and use of this enzyme
WO2004046293A8 (en) Amphoteric fluorescent whitening agents
AU2002257597A1 (en) Immunomodulating agents from parasitic worms and method for isolation thereof
AP2002002547A0 (en) Calcilytic compounds.
DE69737457D1 (de) O-fukosyltransferase
AR027327A1 (es) Procedimiento para la separacion einzimatica de racematos de derivados de aminometil-aril-ciclohezanol
AU2002230579A1 (en) Calcilytic compounds
GB0115382D0 (en) Mutant
AU2002258437A1 (en) Peptide deformylase inhibitors
AU2003287945A8 (en) Mutant protein and refolding method
AU2003304490A1 (en) Peptide deformylase inhibitors
AU2003222462A1 (en) Method of performing soojichim acupuncture using bodycopy
DE50212676D1 (de) Exopolysaccharidproduktion
NO20020064D0 (no) Chlamysin B antibakterielt protein, gen som koder for dette samt et ekspresjonssystem for dette

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties