MXPA02006614A - Promotor mutante apre. - Google Patents

Promotor mutante apre.

Info

Publication number
MXPA02006614A
MXPA02006614A MXPA02006614A MXPA02006614A MXPA02006614A MX PA02006614 A MXPA02006614 A MX PA02006614A MX PA02006614 A MXPA02006614 A MX PA02006614A MX PA02006614 A MXPA02006614 A MX PA02006614A MX PA02006614 A MXPA02006614 A MX PA02006614A
Authority
MX
Mexico
Prior art keywords
mutant
apre promoter
promoter
mutant apre
apre
Prior art date
Application number
MXPA02006614A
Other languages
English (en)
Inventor
Fernando Valle
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Publication of MXPA02006614A publication Critical patent/MXPA02006614A/es

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
    • C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
    • C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
    • C12N9/54—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea bacteria being Bacillus

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biomedical Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)

Abstract

La presente invencion proporciona un promotor mutante aprE y metodos para la produccion de una proteina deseada en una celula huesped Bacillus, que comprende el promotor mutante aprE. La presente invencion proporciona la secuencia de un promotor mutante preferido aprE que proporciona un incremento de 100 veces en la produccion de una proteina de Bacillus subtilis.
MXPA02006614A 2000-01-07 2000-12-21 Promotor mutante apre. MXPA02006614A (es)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/479,494 US6509185B1 (en) 2000-01-07 2000-01-07 Mutant aprE promotor
PCT/US2000/035312 WO2001051643A1 (en) 2000-01-07 2000-12-21 Mutant apre promoter

Publications (1)

Publication Number Publication Date
MXPA02006614A true MXPA02006614A (es) 2004-09-10

Family

ID=23904251

Family Applications (1)

Application Number Title Priority Date Filing Date
MXPA02006614A MXPA02006614A (es) 2000-01-07 2000-12-21 Promotor mutante apre.

Country Status (12)

Country Link
US (2) US6509185B1 (es)
EP (1) EP1244794B1 (es)
JP (1) JP4870306B2 (es)
KR (1) KR20020073497A (es)
CN (2) CN1313615C (es)
AT (1) ATE374822T1 (es)
AU (1) AU2739001A (es)
CA (1) CA2396250C (es)
DE (1) DE60036641T2 (es)
DK (1) DK1244794T3 (es)
MX (1) MXPA02006614A (es)
WO (1) WO2001051643A1 (es)

Families Citing this family (45)

* Cited by examiner, † Cited by third party
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US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor
EP1472347B1 (en) * 2002-02-15 2011-01-12 Danisco US Inc. Improved protein expression in bacillus subtilis
CA2915148C (en) 2002-03-29 2018-03-13 Eugenio Ferrari Enhanced protein expression in bacillus
EP2152732B1 (en) 2007-05-10 2012-03-21 Danisco US Inc. A modified secretion system to increase expression of polypeptides in bacteria
US20090048136A1 (en) * 2007-08-15 2009-02-19 Mcdonald Hugh C Kappa-carrageenase and kappa-carrageenase-containing compositions
EP2195435B1 (en) 2007-09-12 2012-10-10 Danisco US Inc. Trichoderma promoter
RU2515112C2 (ru) 2007-12-21 2014-05-10 ДАНИСКО ЮЭс ИНК. Улучшенная продукция белка в bacillus
AR076941A1 (es) 2009-06-11 2011-07-20 Danisco Us Inc Cepa de bacillus para una mayor produccion de proteina
US20140329309A1 (en) 2011-12-09 2014-11-06 Danisco Us Inc. Ribosomal Promoters for Production in Microorganisms
US10683528B2 (en) * 2014-12-16 2020-06-16 Danisco Us Inc Enhanced protein expression
WO2017075195A1 (en) 2015-10-30 2017-05-04 Danisco Us Inc Enhanced protein expression and methods thereof
EP3380496B1 (en) 2015-12-23 2020-05-20 Danisco US Inc. Enhanced protein production and methods thereof
DK3419991T3 (da) 2016-03-04 2023-01-16 Danisco Us Inc Modificerede ribosomale genpromotorer til proteinproduktion i mikroorganismer
MX2019007335A (es) 2016-12-21 2019-09-09 Dupont Nutrition Biosci Aps Métodos para usar serina proteasas termoestables.
DK3571217T3 (da) 2017-01-23 2023-09-18 Danisco Us Inc Fremgangsmåder og sammensætninger til opnåelse af naturlig kompetence i bacillus-værtsceller
KR102375732B1 (ko) 2017-02-24 2022-03-16 다니스코 유에스 인크. 바실러스 리체니포르미스에서 단백질 생산을 증가시키기 위한 조성물 및 방법
EP3583210B1 (en) 2017-03-15 2021-07-07 Danisco US Inc. Trypsin-like serine proteases and uses thereof
BR112019018969A2 (pt) 2017-03-15 2020-04-22 Dupont Nutrition Biosci Aps serina proteases similar à tripsina e usos das mesmas
WO2018169780A1 (en) 2017-03-15 2018-09-20 Dupont Nutrition Biosciences Aps Methods of using an archaeal serine protease
FI3607097T3 (fi) 2017-04-07 2023-09-11 Dupont Nutrition Biosci Aps BEETA-GALAKTOSIDAASEJA JA LAKTAASEJA ILMAN p-NITROBENTSYYLIESTERASISIVUAKTIIVISUUTTA TUOTTAVIA BACILLUS-ISÄNTÄSOLUJA
CN107142259A (zh) * 2017-05-25 2017-09-08 中国科学院上海高等研究院 一种表达外源基因的启动子及其应用
WO2019040412A1 (en) 2017-08-23 2019-02-28 Danisco Us Inc METHODS AND COMPOSITIONS FOR EFFICIENT GENETIC MODIFICATION OF BACILLUS LICHENIFORMIS STRAINS
JP7218985B2 (ja) 2017-09-13 2023-02-07 ダニスコ・ユーエス・インク バチルス属（Ｂａｃｉｌｌｕｓ）におけるタンパク質産生の増加のための改変５’－非翻訳領域（ＵＴＲ）配列
CN108118058B (zh) * 2017-12-29 2021-06-29 苏州金唯智生物科技有限公司 一种改进的启动子及其应用
CN108118059B (zh) * 2017-12-30 2021-03-19 苏州金唯智生物科技有限公司 一种改进的启动子及其组成的载体和应用
WO2019135868A1 (en) 2018-01-03 2019-07-11 Danisco Us Inc Mutant and genetically modified bacillus cells and methods thereof for increased protein production
BR112021000116A2 (pt) 2018-07-06 2021-04-06 Dupont Nutrition Biosciences Aps Aditivos de alimento contendo xilanase para alimento de animal à base de cereal
EP3887526A1 (en) * 2018-11-28 2021-10-06 Danisco US Inc. Novel promoter sequences and methods thereof for enhanced protein production in bacillus cells
US20220186177A1 (en) 2019-02-20 2022-06-16 Basf Se Industrial fermentation process for bacillus using defined medium and magnesium feed
CN114127256A (zh) 2019-02-20 2022-03-01 巴斯夫欧洲公司 用确定成分培养基和微量元素补料的芽孢杆菌工业发酵工艺
CA3136113A1 (en) 2019-04-05 2020-10-08 Danisco Us Inc. Methods for polynucleotide integration into the genome of bacillus using dual circular recombinant dna constructs and compositions thereof
MX2021012158A (es) 2019-04-05 2022-01-06 Danisco Us Inc Métodos para la integración de una secuencia de adn donante en el genoma de bacillus usando constructos de adn recombinante lineal y composiciones de los mismos.
WO2021030267A1 (en) 2019-08-14 2021-02-18 Danisco Us Inc Compositions and methods for increased protein production in bacillus licheniformis
CA3155372A1 (en) 2019-10-28 2021-05-06 Sharief Barends Microbial host cells for the production of heterologous cyanuric acid hydrolases and biuret hydrolases
CN115335503A (zh) 2019-11-11 2022-11-11 丹尼斯科美国公司 用于增强芽孢杆菌属细胞中蛋白质产生的组合物和方法
JP2023502967A (ja) 2019-11-19 2023-01-26 ダニスコ・ユーエス・インク バチルス（Ｂａｃｉｌｌｕｓ）のゲノムを改変するための、選択マーカーを使用しない方法及びその組成物
US20230340442A1 (en) 2020-01-15 2023-10-26 Danisco Us Inc. Compositions and methods for enhanced protein production in bacillus licheniformis
WO2021224152A1 (en) 2020-05-05 2021-11-11 Basf Se Improving expression in fermentation processes
CN113755492B (zh) * 2020-07-20 2023-05-30 中国科学院天津工业生物技术研究所 丙酮酸羧化酶基因启动子的突变体及其应用
CN116897160A (zh) 2021-02-22 2023-10-17 丹尼斯科美国公司 在色素缺陷型芽孢杆菌属细胞中产生目的蛋白的方法和组合物
CN117769597A (zh) 2021-05-24 2024-03-26 丹尼斯科美国公司 用于增强芽孢杆菌属细胞中蛋白质产生的组合物和方法
WO2022269084A1 (en) 2021-06-24 2022-12-29 Basf Se Improved bacillus host cell with altered rema/remb protein
EP4359424A1 (en) 2021-06-24 2024-05-01 Basf Se Improved bacillus production host
WO2023091878A1 (en) 2021-11-16 2023-05-25 Danisco Us Inc. Compositions and methods for enhanced protein production in bacillus cells
WO2023233996A1 (ja) * 2022-05-31 2023-12-07 パナソニックＩｐマネジメント株式会社 改変プロモーター、発現ベクター、微生物、物質の生産方法、改変シアノバクテリア及び改変プロモーターの作製方法

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4828994A (en) * 1984-09-21 1989-05-09 Genex Corporation Bacillus strains with reduced extracellular protease levels
DK87092D0 (da) * 1992-07-02 1992-07-02 Novo Nordisk As Nyt enzym
US6509185B1 (en) * 2000-01-07 2003-01-21 Genencor International, Inc. Mutant aprE promotor

Also Published As

Publication number Publication date
ATE374822T1 (de) 2007-10-15
JP4870306B2 (ja) 2012-02-08
US6509185B1 (en) 2003-01-21
EP1244794A1 (en) 2002-10-02
US20030148461A1 (en) 2003-08-07
EP1244794B1 (en) 2007-10-03
CN101054588A (zh) 2007-10-17
AU2739001A (en) 2001-07-24
JP2003519492A (ja) 2003-06-24
KR20020073497A (ko) 2002-09-26
CN1313615C (zh) 2007-05-02
DK1244794T3 (da) 2008-01-28
CA2396250A1 (en) 2001-07-19
DE60036641D1 (de) 2007-11-15
CN1425071A (zh) 2003-06-18
WO2001051643A1 (en) 2001-07-19
CA2396250C (en) 2009-02-24
DE60036641T2 (de) 2008-07-03
US6911322B2 (en) 2005-06-28

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