DE60032227D1 - Methode zur synthese von polypeptiden in zell-freien systemen - Google Patents

Methode zur synthese von polypeptiden in zell-freien systemen

Info

Publication number
DE60032227D1
DE60032227D1 DE60032227T DE60032227T DE60032227D1 DE 60032227 D1 DE60032227 D1 DE 60032227D1 DE 60032227 T DE60032227 T DE 60032227T DE 60032227 T DE60032227 T DE 60032227T DE 60032227 D1 DE60032227 D1 DE 60032227D1
Authority
DE
Germany
Prior art keywords
synthesis
components
cell
free systems
concentrations
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60032227T
Other languages
English (en)
Other versions
DE60032227T2 (de
Inventor
Sergey Vladimirovich Biryukov
Peter Nikolaevich Simonenko
Vladimir Anatolievich Shirokov
Alexander Sergeyevich Spirin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
Institute of Protein Research
Original Assignee
Roche Diagnostics GmbH
Institute of Protein Research
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH, Institute of Protein Research filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of DE60032227D1 publication Critical patent/DE60032227D1/de
Publication of DE60032227T2 publication Critical patent/DE60032227T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
DE60032227T 1999-03-25 2000-03-22 Methode zur synthese von polypeptiden in zell-freien systemen Expired - Lifetime DE60032227T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
RU99106754/13A RU2169154C2 (ru) 1999-03-25 1999-03-25 Способ получения полипептидов в бесклеточной системе
RU99106754 1999-03-25
PCT/EP2000/002508 WO2000058493A1 (en) 1999-03-25 2000-03-22 Method for synthesis of polypeptides in cell-free systems

Publications (2)

Publication Number Publication Date
DE60032227D1 true DE60032227D1 (de) 2007-01-18
DE60032227T2 DE60032227T2 (de) 2007-12-06

Family

ID=20217990

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60032227T Expired - Lifetime DE60032227T2 (de) 1999-03-25 2000-03-22 Methode zur synthese von polypeptiden in zell-freien systemen

Country Status (9)

Country Link
US (1) US6783957B1 (de)
EP (1) EP1165826B1 (de)
JP (1) JP3836679B2 (de)
AT (1) ATE347610T1 (de)
CA (1) CA2365237C (de)
DE (1) DE60032227T2 (de)
ES (1) ES2277832T3 (de)
RU (1) RU2169154C2 (de)
WO (1) WO2000058493A1 (de)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE10011209A1 (de) 2000-03-08 2001-09-13 Roche Diagnostics Gmbh Matrix-Reaktor und Verfahren zur Herstellung von Produkten in diesem Reaktor
DE10137792A1 (de) * 2001-08-06 2003-02-27 Erdmann Volker Verfahren zur Genexpression
EP1295894B1 (de) 2001-09-25 2011-09-14 F. Hoffmann-La Roche AG In vitro Verfahren zur sequenzspezifischen Biotinylierung von Polypeptiden
CA2463719A1 (en) 2003-04-05 2004-10-05 F. Hoffmann-La Roche Ag Nucleotide analogs with six membered rings
US7341852B2 (en) * 2003-07-18 2008-03-11 The Board Of Trustees Of The Leland Stanford Junior University Methods of decoupling reaction scale and protein synthesis yield in batch mode
JPWO2005123936A1 (ja) * 2004-06-15 2008-04-10 三菱化学株式会社 ポリペプチド合成方法
JP4513495B2 (ja) 2004-10-15 2010-07-28 株式会社島津製作所 昆虫細胞由来抽出液を用いた無細胞タンパク質合成方法
US20070117179A1 (en) * 2005-09-27 2007-05-24 Invitrogen Corporation In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles
WO2008106660A2 (en) * 2007-03-01 2008-09-04 Invitrogen Corporation Isolated phospholipid-protein particles
US9399670B2 (en) 2009-02-20 2016-07-26 Hoffmann-La Roche Inc. Method for obtaining immunoglobulin encoding nucleic acid
KR20140064857A (ko) 2011-09-21 2014-05-28 에프. 호프만-라 로슈 아게 TaqMan 프로브와 조합된 다중 PCR 에 의해 단일 항체 생성 세포로부터 FAB 단편을 수득하는 방법
US9528137B2 (en) 2013-03-15 2016-12-27 Northwestern University Methods for cell-free protein synthesis
EP3277808A4 (de) 2015-03-30 2019-05-22 Greenlight Biosciences, Inc. Zellfreie herstellung von ribonukleinsäure
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
RU199182U1 (ru) * 2019-12-10 2020-08-19 Федеральное государственное бюджетное учреждение науки Институт белка Российской академии наук (ИБ РАН) Устройство для синтеза белков в бесклеточной системе

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US4668624A (en) 1979-02-28 1987-05-26 E.I. Du Pont De Nemours And Company Protein translation method
US5135853A (en) 1983-07-22 1992-08-04 Rensselaer Polytechnic Institute Three compartment bioreactor and method of use
SU1618761A1 (ru) 1987-04-29 1991-01-07 Институт Белка Ан Ссср Способ получени пептидов и белков в бесклеточной системе трансл ции
US4937190A (en) 1987-10-15 1990-06-26 Wisconsin Alumni Research Foundation Translation enhancer
US5478730A (en) 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
SU1705302A1 (ru) 1988-12-22 1992-01-15 Институт Белка Ан Ссср Способ препаративной экспрессии генов в бесклеточной системе сопр женной транскрипции/трансл ции
DE3914956A1 (de) 1989-05-06 1990-11-22 Fischer Karl Heinz Verfahren zur beschleunigung des stoffaustauschs eines kontinuierlichen bioreaktors und vorrichtung zur durchfuehrung dieses verfahrens
WO1991002075A1 (fr) 1989-07-31 1991-02-21 Institut Belka Akademii Nauk Sssr Procede d'obtention de polypeptides dans un systeme de translation exempt de cellules
US6399323B1 (en) 1989-07-31 2002-06-04 Institute For Protein Research Method of preparing polypeptides in a cell-free translation system
AU2252092A (en) 1991-06-24 1993-01-25 Research Foundation Of The State University Of New York, The De novo cell-free synthesis of picornavirus
JPH0576381A (ja) 1991-09-19 1993-03-30 Hitachi Ltd ポリペプチド合成方法
JP2904583B2 (ja) 1991-10-11 1999-06-14 プロメガ・コーポレーション 真核無細胞抽出物中での転写と翻訳の共役
AU4843893A (en) 1992-09-02 1994-03-29 Scripps Research Institute, The Coupled isothermal polynucleotide amplification and translation system
BR9300343A (pt) 1993-02-09 1994-09-27 Semer S A Secadora multifunções
US5434079A (en) 1993-02-12 1995-07-18 The United States Of America As Represented By The Department Of Health And Human Services Apparatus and process for continuous in vitro synthesis of proteins
JPH0731494A (ja) 1993-07-14 1995-02-03 Hitachi Ltd 無細胞系におけるタンパク質の生産方法及び装置
US5324428A (en) 1993-07-19 1994-06-28 Spectrum Medical Industries, Inc. Disposable dialysis apparatus
JPH0775592A (ja) 1993-09-07 1995-03-20 Hitachi Ltd 細胞抽出液による蛋白質合成方法及び装置
CA2170738C (en) 1993-09-22 1999-03-09 Carl Clark Device and method for dialyzing samples
KR0131166B1 (ko) 1994-05-04 1998-04-11 최차용 무세포시스템에서 단백질을 제조하는 방법
JPH1080295A (ja) 1996-07-15 1998-03-31 Nippon Flour Mills Co Ltd 無細胞タンパク合成系によるタンパクの合成方法及び装置
DE59611424D1 (de) 1996-07-16 2007-05-03 Qiagen North American Holdings Verfahren zur herstellung komplexer multienzymatischer lagerstabiler reaktionsgemische und deren verwendung
DE59810647D1 (de) 1997-07-31 2004-03-04 Roche Diagnostics Gmbh Verfahren und Vorrichtung zur Durchführung biochemischer Reaktionen
RU2148649C1 (ru) * 1998-03-31 2000-05-10 Институт белка РАН Способ получения полипептидов в бесклеточной системе (варианты) и устройство для его осуществления

Also Published As

Publication number Publication date
RU2169154C2 (ru) 2001-06-20
ES2277832T3 (es) 2007-08-01
ATE347610T1 (de) 2006-12-15
EP1165826B1 (de) 2006-12-06
CA2365237A1 (en) 2000-10-05
WO2000058493A1 (en) 2000-10-05
JP2002539840A (ja) 2002-11-26
DE60032227T2 (de) 2007-12-06
EP1165826A1 (de) 2002-01-02
US6783957B1 (en) 2004-08-31
JP3836679B2 (ja) 2006-10-25
CA2365237C (en) 2008-01-22

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