DE69931479D1 - Verfahren zur herstellung von polypeptiden in einem zellfreien system und vorrichtung zu deren ausführung - Google Patents

Verfahren zur herstellung von polypeptiden in einem zellfreien system und vorrichtung zu deren ausführung

Info

Publication number
DE69931479D1
DE69931479D1 DE69931479T DE69931479T DE69931479D1 DE 69931479 D1 DE69931479 D1 DE 69931479D1 DE 69931479 T DE69931479 T DE 69931479T DE 69931479 T DE69931479 T DE 69931479T DE 69931479 D1 DE69931479 D1 DE 69931479D1
Authority
DE
Germany
Prior art keywords
fraction
cell
polypeptides
molecular weight
free system
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE69931479T
Other languages
English (en)
Other versions
DE69931479T2 (de
Inventor
Sergey Vladimirovich Biryukov
Peter Nikolayevich Simonenko
Vladimir Anatolievich Shirokov
Sergey Gennadievich Majorov
Alexander Sergeyevich Spirin
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Roche Diagnostics GmbH
INST OF PROTEIN RES RUSSIAN AC
Original Assignee
Roche Diagnostics GmbH
INST OF PROTEIN RES RUSSIAN AC
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Roche Diagnostics GmbH, INST OF PROTEIN RES RUSSIAN AC filed Critical Roche Diagnostics GmbH
Application granted granted Critical
Publication of DE69931479D1 publication Critical patent/DE69931479D1/de
Publication of DE69931479T2 publication Critical patent/DE69931479T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/34—Internal compartments or partitions
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M23/00—Constructional details, e.g. recesses, hinges
    • C12M23/02—Form or structure of the vessel
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/04—Filters; Permeable or porous membranes or plates, e.g. dialysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/16—Hollow fibers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M29/00—Means for introduction, extraction or recirculation of materials, e.g. pumps
    • C12M29/18—External loop; Means for reintroduction of fermented biomass or liquid percolate
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Genetics & Genomics (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Sustainable Development (AREA)
  • Clinical Laboratory Science (AREA)
  • Immunology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Molecular Biology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Separation Using Semi-Permeable Membranes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
DE69931479T 1998-03-31 1999-03-27 Verfahren zur herstellung von polypeptiden in einem zellfreien system und vorrichtung zu deren ausführung Expired - Lifetime DE69931479T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
RU98105294 1998-03-31
RU98105294/13A RU2148649C1 (ru) 1998-03-31 1998-03-31 Способ получения полипептидов в бесклеточной системе (варианты) и устройство для его осуществления
PCT/EP1999/002124 WO1999050436A1 (en) 1998-03-31 1999-03-27 Method of preparing polypeptides in cell-free system and device for its realization

Publications (2)

Publication Number Publication Date
DE69931479D1 true DE69931479D1 (de) 2006-06-29
DE69931479T2 DE69931479T2 (de) 2007-02-22

Family

ID=20203746

Family Applications (1)

Application Number Title Priority Date Filing Date
DE69931479T Expired - Lifetime DE69931479T2 (de) 1998-03-31 1999-03-27 Verfahren zur herstellung von polypeptiden in einem zellfreien system und vorrichtung zu deren ausführung

Country Status (8)

Country Link
US (2) US6518058B1 (de)
EP (1) EP1068348B1 (de)
JP (1) JP3813817B2 (de)
AT (1) ATE327341T1 (de)
DE (1) DE69931479T2 (de)
ES (1) ES2265685T3 (de)
RU (1) RU2148649C1 (de)
WO (1) WO1999050436A1 (de)

Families Citing this family (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
RU2169154C2 (ru) 1999-03-25 2001-06-20 Институт белка РАН Способ получения полипептидов в бесклеточной системе
EP1061128B1 (de) * 1999-06-18 2004-10-13 Roche Diagnostics GmbH Verfahren und Vorrichtung zur Durchführung biochemischer Reaktionen mit hohem Durchsatz
US20040115760A1 (en) 1999-06-18 2004-06-17 Thomas Metzler Method and device for carrying out biochemical reactions with a high throughput
DE10137792A1 (de) * 2001-08-06 2003-02-27 Erdmann Volker Verfahren zur Genexpression
EP1295894B1 (de) 2001-09-25 2011-09-14 F. Hoffmann-La Roche AG In vitro Verfahren zur sequenzspezifischen Biotinylierung von Polypeptiden
JP2004042012A (ja) * 2001-10-26 2004-02-12 Nec Corp 分離装置、分析システム、分離方法および分離装置の製造方法
JP2005007352A (ja) * 2003-06-20 2005-01-13 Sharp Corp 粒子の分離方法及び分離装置並びに検出装置
DK1730313T3 (da) * 2004-03-25 2013-01-07 Univ Leland Stanford Junior Forøget proteinekspressionsudbytte i cellefri proteinsyntesesystemer ved tilsætning af skumdæmpende midler
US20070224662A1 (en) * 2004-12-17 2007-09-27 Jun Luo Post-translational modification of proteins in cell-free expression systems
US20080187963A1 (en) * 2005-07-04 2008-08-07 Max-Planck Gesellschaft Zur Foerderung Der Wissenschaften E.V. Use of Lepa for Improving the Accuracy of Protein Synthesis in Vitro
US20080248565A1 (en) * 2007-03-01 2008-10-09 Invitrogen Corporation Isolated phospholipid-protein particles
US20070117179A1 (en) * 2005-09-27 2007-05-24 Invitrogen Corporation In vitro protein synthesis systems for membrane proteins that include adolipoproteins and phospholipid-adolipoprotein particles
CA2656322A1 (en) * 2006-06-29 2008-01-03 Searete Llc Methods for arbitrary peptide synthesis
US7993873B2 (en) 2006-06-29 2011-08-09 The Invention Science Fund I, Llc Apparatus for arbitrary peptide synthesis
US9399670B2 (en) 2009-02-20 2016-07-26 Hoffmann-La Roche Inc. Method for obtaining immunoglobulin encoding nucleic acid
DE102010048422B4 (de) * 2010-10-15 2016-01-07 Thomas Melin Verfahren zur Produktisolierung und Substratzufuhr in einem Bioreaktor
JP2014527825A (ja) 2011-09-21 2014-10-23 エフ．ホフマン−ラ ロシュ アーゲーＦ． Ｈｏｆｆｍａｎｎ−Ｌａ Ｒｏｃｈｅ Ａｋｔｉｅｎｇｅｓｅｌｌｓｃｈａｆｔ ＴａｑＭａｎプローブと組み合わせたマルチプレックスＰＣＲにより単一の抗体産生細胞からＦａｂ断片を得るための方法
US9528137B2 (en) 2013-03-15 2016-12-27 Northwestern University Methods for cell-free protein synthesis
US11530432B2 (en) 2018-03-19 2022-12-20 Northwestern University Compositions and methods for rapid in vitro synthesis of bioconjugate vaccines in vitro via production and N-glycosylation of protein carriers in detoxified prokaryotic cell lysates
US11725224B2 (en) 2018-04-16 2023-08-15 Northwestern University Methods for co-activating in vitro non-standard amino acid (nsAA) incorporation and glycosylation in crude cell lysates
US11814621B2 (en) 2018-06-01 2023-11-14 Northwestern University Expanding the chemical substrates for genetic code reprogramming
RU199182U1 (ru) * 2019-12-10 2020-08-19 Федеральное государственное бюджетное учреждение науки Институт белка Российской академии наук (ИБ РАН) Устройство для синтеза белков в бесклеточной системе
CN113512088B (zh) * 2021-05-08 2022-09-27 苏州珀罗汀生物技术有限公司 一种蛋白连续生产装置及方法

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE2726313C3 (de) * 1977-06-10 1980-02-07 Battelle-Institut E.V., 6000 Frankfurt Verfahren zur in-vitro-Biosynthese von Hormonen, insbesondere von Insulin
US4341631A (en) * 1979-09-07 1982-07-27 Union Carbide Corporation Ultrafiltration and reverse osmosis device comprising plural carbon tubes bonded together
US4786597A (en) * 1981-04-15 1988-11-22 University Patents, Inc. Method and apparatus for conducting catalytic reactions with simultaneous product separation and recovery
US5135853A (en) * 1983-07-22 1992-08-04 Rensselaer Polytechnic Institute Three compartment bioreactor and method of use
US4956289A (en) * 1987-03-16 1990-09-11 Brunswick Corporation Thin film membrane enzyme reactor and method of using same
US5605835A (en) * 1988-05-23 1997-02-25 Regents Of The University Of Minnesota Bioreactor device with application as a bioartificial liver
US5478730A (en) * 1988-12-21 1995-12-26 Institute Of Protein Research Method of preparing polypeptides in cell-free translation system
US6399323B1 (en) * 1989-07-31 2002-06-04 Institute For Protein Research Method of preparing polypeptides in a cell-free translation system
US5656421A (en) * 1990-02-15 1997-08-12 Unisyn Technologies, Inc. Multi-bioreactor hollow fiber cell propagation system and method
US5198110A (en) * 1990-07-02 1993-03-30 Asahi Medical Co., Ltd. Bundle of permselective hollow fibers and a fluid separator containing the same
US5366625A (en) * 1992-03-04 1994-11-22 Pedersen Steven K Cartridge of hybrid unitary wafers of hollow fiber membranes and module containing a stack of post-potted cartridges
US5434079A (en) 1993-02-12 1995-07-18 The United States Of America As Represented By The Department Of Health And Human Services Apparatus and process for continuous in vitro synthesis of proteins
JPH0768136A (ja) * 1993-09-02 1995-03-14 Tsuchiya Mfg Co Ltd 中空糸膜型分離モジュ−ル及びその製造方法
DE4331596A1 (de) * 1993-09-17 1995-03-23 Boehringer Mannheim Gmbh Verfahren zur quantitativen Analyse von Probenflüssigkeiten
KR0131166B1 (ko) * 1994-05-04 1998-04-11 최차용 무세포시스템에서 단백질을 제조하는 방법

Also Published As

Publication number Publication date
US20040086959A1 (en) 2004-05-06
EP1068348B1 (de) 2006-05-24
RU2148649C1 (ru) 2000-05-10
JP2002509730A (ja) 2002-04-02
EP1068348A1 (de) 2001-01-17
ES2265685T3 (es) 2007-02-16
US6518058B1 (en) 2003-02-11
JP3813817B2 (ja) 2006-08-23
DE69931479T2 (de) 2007-02-22
WO1999050436A1 (en) 1999-10-07
ATE327341T1 (de) 2006-06-15

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