DK1730313T3 - Forøget proteinekspressionsudbytte i cellefri proteinsyntesesystemer ved tilsætning af skumdæmpende midler - Google Patents
Forøget proteinekspressionsudbytte i cellefri proteinsyntesesystemer ved tilsætning af skumdæmpende midlerInfo
- Publication number
- DK1730313T3 DK1730313T3 DK05733219.9T DK05733219T DK1730313T3 DK 1730313 T3 DK1730313 T3 DK 1730313T3 DK 05733219 T DK05733219 T DK 05733219T DK 1730313 T3 DK1730313 T3 DK 1730313T3
- Authority
- DK
- Denmark
- Prior art keywords
- cell
- addition
- antifoam agents
- synthesis systems
- expression yield
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P19/00—Preparation of compounds containing saccharide radicals
- C12P19/26—Preparation of nitrogen-containing carbohydrates
- C12P19/28—N-glycosides
- C12P19/30—Nucleotides
- C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- Genetics & Genomics (AREA)
- Biotechnology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Engineering & Computer Science (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Biochemistry (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Biomedical Technology (AREA)
- Physics & Mathematics (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Physical Or Chemical Processes And Apparatus (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US55673604P | 2004-03-25 | 2004-03-25 | |
| PCT/US2005/009342 WO2005098048A1 (en) | 2004-03-25 | 2005-03-21 | Protein expression yield enhancement in cell-free protein synthesis systems by addition of antifoam agents |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| DK1730313T3 true DK1730313T3 (da) | 2013-01-07 |
Family
ID=35125089
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| DK05733219.9T DK1730313T3 (da) | 2004-03-25 | 2005-03-21 | Forøget proteinekspressionsudbytte i cellefri proteinsyntesesystemer ved tilsætning af skumdæmpende midler |
Country Status (14)
| Country | Link |
|---|---|
| US (1) | US8298759B2 (da) |
| EP (1) | EP1730313B1 (da) |
| JP (1) | JP4829215B2 (da) |
| KR (1) | KR101229849B1 (da) |
| CN (1) | CN1934276A (da) |
| AU (1) | AU2005230916B2 (da) |
| CA (1) | CA2560504C (da) |
| DK (1) | DK1730313T3 (da) |
| EA (1) | EA010837B1 (da) |
| ES (1) | ES2394443T3 (da) |
| MX (1) | MXPA06010918A (da) |
| NO (1) | NO331586B1 (da) |
| NZ (1) | NZ549523A (da) |
| WO (1) | WO2005098048A1 (da) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7341852B2 (en) | 2003-07-18 | 2008-03-11 | The Board Of Trustees Of The Leland Stanford Junior University | Methods of decoupling reaction scale and protein synthesis yield in batch mode |
| US8183010B2 (en) | 2005-10-31 | 2012-05-22 | The Board Of Trustees Of The Leland Stanford Junior University | Cell-free synthesis of membrane bound polypeptides |
| WO2008002661A2 (en) * | 2006-06-28 | 2008-01-03 | The Board Of Trustees Of The Leland Stanford Junior University | Fusion protein constructs |
| EP2376619A4 (en) | 2008-12-15 | 2012-07-04 | Greenlight Biosciences Inc | METHODS FOR CONTROLLING FLOWS IN METABOLIC PATHWAYS |
| KR20130071433A (ko) | 2010-05-07 | 2013-06-28 | 그린라이트 바이오사이언시스, 아이엔씨. | 효소의 재배치를 통한 대사 경로에서의 흐름의 제어 방법 |
| US8916358B2 (en) | 2010-08-31 | 2014-12-23 | Greenlight Biosciences, Inc. | Methods for control of flux in metabolic pathways through protease manipulation |
| JP2014526250A (ja) | 2011-09-09 | 2014-10-06 | グリーンライト バイオサイエンシーズ インコーポレーテッド | カルバペネムの無細胞調製 |
| JP2016504034A (ja) | 2012-12-21 | 2016-02-12 | グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． | メタンを燃料、ピルビン酸またはイソブタノールに変換する無細胞システム |
| WO2014197655A1 (en) * | 2013-06-05 | 2014-12-11 | Greenlight Biosciences, Inc. | Control of metabolic flux in cell-free biosynthetic systems |
| AU2014306074B2 (en) | 2013-08-05 | 2018-08-30 | Greenlight Biosciences, Inc. | Engineered proteins with a protease cleavage site |
| BR112016002597B1 (pt) | 2013-08-19 | 2021-03-02 | Dow Global Technologies Llc | método para polimerizar um ou mais polímeros à base de etileno |
| KR101671688B1 (ko) | 2014-06-12 | 2016-11-03 | 충남대학교산학협력단 | 효소를 이용하여 pH를 조절하는 무세포 단백질 합성 방법 |
| KR20230048162A (ko) | 2015-03-30 | 2023-04-10 | 그린라이트 바이오사이언시스, 아이엔씨. | 리보핵산의 무세포 생산 |
| CN105255818A (zh) * | 2015-11-13 | 2016-01-20 | 肇庆大华农生物药品有限公司 | 一种改良的pk-15细胞传代培养方法及其应用 |
| WO2017176963A1 (en) | 2016-04-06 | 2017-10-12 | Greenlight Biosciences, Inc. | Cell-free production of ribonucleic acid |
| US11951165B2 (en) | 2016-12-30 | 2024-04-09 | Vaxcyte, Inc. | Conjugated vaccine carrier proteins |
| JP7186166B2 (ja) | 2016-12-30 | 2022-12-08 | バックスサイト・インコーポレイテッド | 非天然アミノ酸とのポリペプチド抗原接合体 |
| MX2019008159A (es) | 2017-01-06 | 2019-12-09 | Greenlight Biosciences Inc | Producción acelular de azúcares. |
| CN106834391A (zh) * | 2017-01-18 | 2017-06-13 | 天津大学 | 一种基于人工多酶生化反应网络合成蛋白质的配方和方法 |
| CN108396034A (zh) * | 2017-02-06 | 2018-08-14 | 武汉臻智生物科技有限公司 | 一种提高无细胞体系蛋白质合成的方法 |
| WO2019035916A1 (en) | 2017-08-15 | 2019-02-21 | Northwestern University | DESIGN OF PROTEIN GLYCOSYLATION SITES BY RAPID EXPRESSION AND CHARACTERIZATION OF N-GLYCOSYLTRANSFERASES |
| MX2020003841A (es) | 2017-10-11 | 2020-11-06 | Greenlight Biosciences Inc | Métodos y composiciones para la producción de nucleósido trifosfatos y ácidos ribonucleicos. |
| US11673921B2 (en) | 2017-11-10 | 2023-06-13 | Northwestern University | Cell-free protein synthesis platform derived from cellular extracts of Vibrio natriegens |
| WO2019204346A1 (en) | 2018-04-16 | 2019-10-24 | Northwestern University | METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES |
| US11814621B2 (en) | 2018-06-01 | 2023-11-14 | Northwestern University | Expanding the chemical substrates for genetic code reprogramming |
| KR20220044212A (ko) | 2019-08-09 | 2022-04-06 | 넛크래커 테라퓨틱스 인코포레이티드 | 미세유체 장치 및 이의 사용 방법 |
Family Cites Families (12)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3990905A (en) * | 1976-02-09 | 1976-11-09 | Nalco Chemical Company | Food process antifoam |
| US5547841A (en) * | 1987-10-08 | 1996-08-20 | The Mclean Hospital Corporation | In vitro method for screening for drugs that inhibit production or degradation of human A4-amyloid |
| JPH04200390A (ja) | 1990-11-30 | 1992-07-21 | Shigeyuki Yokoyama | 無細胞ポリペプチド合成系によるポリペプチドの製造方法 |
| US5223412A (en) * | 1991-02-21 | 1993-06-29 | Genencor International, Inc. | Cell-free and whole cell ice nucleators and process for their production |
| US5856174A (en) * | 1995-06-29 | 1999-01-05 | Affymetrix, Inc. | Integrated nucleic acid diagnostic device |
| RU2148649C1 (ru) * | 1998-03-31 | 2000-05-10 | Институт белка РАН | Способ получения полипептидов в бесклеточной системе (варианты) и устройство для его осуществления |
| ATE265539T1 (de) * | 1998-05-25 | 2004-05-15 | Qiagen Gmbh | Verfahren zur isolierung von ccc plasmid dna |
| AU4325900A (en) * | 1999-03-17 | 2000-10-04 | Board Of Trustees Of The Leland Stanford Junior University | In vitro macromolecule biosynthesis methods using exogenous amino acids and a novel atp regeneration system |
| US6337191B1 (en) * | 1999-03-22 | 2002-01-08 | The Board Of Trustees Of The Leland Stanford Junior University | Vitro protein synthesis using glycolytic intermediates as an energy source |
| WO2003097829A1 (en) * | 2002-05-22 | 2003-11-27 | Riken | Process for producing protein in cell-free protein synthesis system using thioredoxin fused protein expression vector |
| EP2345738A1 (en) * | 2002-08-05 | 2011-07-20 | Quanta Biosciences, Inc. | Improved compositions for in vitro amplification of nucleic acids |
| AU2004293798B2 (en) * | 2003-11-20 | 2008-10-23 | The Board Of Trustees Of The Leland Stanford Junior University | Improved methods of in vitro protein synthesis |
-
2005
- 2005-03-21 WO PCT/US2005/009342 patent/WO2005098048A1/en active Application Filing
- 2005-03-21 CA CA2560504A patent/CA2560504C/en active Active
- 2005-03-21 EA EA200601748A patent/EA010837B1/ru unknown
- 2005-03-21 DK DK05733219.9T patent/DK1730313T3/da active
- 2005-03-21 AU AU2005230916A patent/AU2005230916B2/en active Active
- 2005-03-21 ES ES05733219T patent/ES2394443T3/es active Active
- 2005-03-21 EP EP05733219A patent/EP1730313B1/en active Active
- 2005-03-21 MX MXPA06010918A patent/MXPA06010918A/es active IP Right Grant
- 2005-03-21 CN CNA2005800094648A patent/CN1934276A/zh active Pending
- 2005-03-21 JP JP2007505063A patent/JP4829215B2/ja active Active
- 2005-03-21 US US10/599,310 patent/US8298759B2/en active Active
- 2005-03-21 NZ NZ549523A patent/NZ549523A/en unknown
- 2005-03-21 KR KR1020067019493A patent/KR101229849B1/ko active IP Right Grant
-
2006
- 2006-10-19 NO NO20064735A patent/NO331586B1/no unknown
Also Published As
| Publication number | Publication date |
|---|---|
| US8298759B2 (en) | 2012-10-30 |
| WO2005098048A1 (en) | 2005-10-20 |
| US20090042244A1 (en) | 2009-02-12 |
| JP2007530042A (ja) | 2007-11-01 |
| MXPA06010918A (es) | 2006-12-15 |
| AU2005230916B2 (en) | 2012-03-08 |
| JP4829215B2 (ja) | 2011-12-07 |
| EA010837B1 (ru) | 2008-12-30 |
| AU2005230916A1 (en) | 2005-10-20 |
| KR20070011320A (ko) | 2007-01-24 |
| NO331586B1 (no) | 2012-01-30 |
| CA2560504C (en) | 2014-09-16 |
| KR101229849B1 (ko) | 2013-02-05 |
| EP1730313A4 (en) | 2007-04-11 |
| CN1934276A (zh) | 2007-03-21 |
| EP1730313A1 (en) | 2006-12-13 |
| NO20064735L (no) | 2006-10-19 |
| ES2394443T3 (es) | 2013-01-31 |
| EA200601748A1 (ru) | 2007-04-27 |
| EP1730313B1 (en) | 2012-10-17 |
| NZ549523A (en) | 2009-10-30 |
| CA2560504A1 (en) | 2005-10-20 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| DK1730313T3 (da) | Forøget proteinekspressionsudbytte i cellefri proteinsyntesesystemer ved tilsætning af skumdæmpende midler | |
| DK1802579T3 (da) | Derivater af 3-arylaminopyridin | |
| DK1992697T3 (da) | Fremstilling af TNFR-Fc | |
| DK1828164T3 (da) | Fremgangsmåde til fremstilling af N-phenylpyrazol-1-carboxamider | |
| DK1778668T3 (da) | Fremgangsmåde til fremstilling af dihydropteridion | |
| DK1737891T3 (da) | Anti-p-selectin-antistoffer | |
| DE602005011795D1 (de) | Inhibitoren von 11-beta-hydroxysteroiddehydrogenase typ 1 | |
| CY2013046I2 (el) | Καινοτομος χρηση των αντισωματων αντι il-1bhta | |
| DK1708992T3 (da) | Sulfonamidderivater til behandling af sygdomme | |
| DK1853700T3 (da) | Rekombinant co-ekspression af vitamin-k-epoxid-reduktase-underenhed 1 til forbedring af vitamin-k-afhængig proteinekspression | |
| DK1948643T3 (da) | 3-Alkyl-5-(4-alkyl-5-oxo-tetrahydrofuran-2-yl)pyrrolidin-2-on-derivater som mellemprodukter i syntese af renininhibitorer | |
| ATE509011T1 (de) | Pyrimidinderivate als 11beta-hsd1-inhibitoren | |
| DK1587542T3 (da) | Anvendelse af anti-CD100-antistoffer | |
| DK1840125T3 (da) | Mellemprodukter til fremstilling af dioxan-2-alkylcarbamater | |
| DK1833495T3 (da) | Dermatologisk anvendelse af mælkeproteiner | |
| DK1831223T3 (da) | Fremgangsmåde til fremstilling af L-biopterin | |
| DK1645165T3 (da) | Modtagerenhed til reducering af tilbagekobling | |
| DK1519928T3 (da) | Fremgangsmåde til fremstilling af heterocykliske fluoralkenylsulfoner | |
| DK1786913T3 (da) | Fremgangsmåde til fremstilling af diarylcycloalkylderivater | |
| ITMI20030352A1 (it) | Metodo per la sintesi di metiltriossorenio. | |
| DK1833814T3 (da) | Fremgangsmåde til fremstilling af substituerede 2-alkoxycarbonyl-3-aminothiophener | |
| DK1735297T3 (da) | Fremgangsmåde til fremstilling af benzoxathiepinderivater | |
| ATE402145T1 (de) | Synthese von pyrrol-2-carbonsäurenitrilen | |
| DK1591434T3 (da) | Nye 1-methoxy-2-phenyl-ethener anvendelig til fremstiling af 5-carboxaldehyd-2-3-dihydrobenzoxepiner | |
| ES1060527Y (es) | "baliza" |