DK1730313T3 - Forøget proteinekspressionsudbytte i cellefri proteinsyntesesystemer ved tilsætning af skumdæmpende midler - Google Patents

Forøget proteinekspressionsudbytte i cellefri proteinsyntesesystemer ved tilsætning af skumdæmpende midler

Info

Publication number
DK1730313T3
DK1730313T3 DK05733219.9T DK05733219T DK1730313T3 DK 1730313 T3 DK1730313 T3 DK 1730313T3 DK 05733219 T DK05733219 T DK 05733219T DK 1730313 T3 DK1730313 T3 DK 1730313T3
Authority
DK
Denmark
Prior art keywords
cell
addition
antifoam agents
synthesis systems
expression yield
Prior art date
Application number
DK05733219.9T
Other languages
English (en)
Inventor
Alexei M Voloshin
James Robert Swartz
Original Assignee
Univ Leland Stanford Junior
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Leland Stanford Junior filed Critical Univ Leland Stanford Junior
Application granted granted Critical
Publication of DK1730313T3 publication Critical patent/DK1730313T3/da

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/26—Preparation of nitrogen-containing carbohydrates
    • C12P19/28—N-glycosides
    • C12P19/30—Nucleotides
    • C12P19/34—Polynucleotides, e.g. nucleic acids, oligoribonucleotides
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins

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  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
DK05733219.9T 2004-03-25 2005-03-21 Forøget proteinekspressionsudbytte i cellefri proteinsyntesesystemer ved tilsætning af skumdæmpende midler DK1730313T3 (da)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55673604P 2004-03-25 2004-03-25
PCT/US2005/009342 WO2005098048A1 (en) 2004-03-25 2005-03-21 Protein expression yield enhancement in cell-free protein synthesis systems by addition of antifoam agents

Publications (1)

Publication Number Publication Date
DK1730313T3 true DK1730313T3 (da) 2013-01-07

Family

ID=35125089

Family Applications (1)

Application Number Title Priority Date Filing Date
DK05733219.9T DK1730313T3 (da) 2004-03-25 2005-03-21 Forøget proteinekspressionsudbytte i cellefri proteinsyntesesystemer ved tilsætning af skumdæmpende midler

Country Status (14)

Country Link
US (1) US8298759B2 (da)
EP (1) EP1730313B1 (da)
JP (1) JP4829215B2 (da)
KR (1) KR101229849B1 (da)
CN (1) CN1934276A (da)
AU (1) AU2005230916B2 (da)
CA (1) CA2560504C (da)
DK (1) DK1730313T3 (da)
EA (1) EA010837B1 (da)
ES (1) ES2394443T3 (da)
MX (1) MXPA06010918A (da)
NO (1) NO331586B1 (da)
NZ (1) NZ549523A (da)
WO (1) WO2005098048A1 (da)

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US7341852B2 (en) * 2003-07-18 2008-03-11 The Board Of Trustees Of The Leland Stanford Junior University Methods of decoupling reaction scale and protein synthesis yield in batch mode
US8183010B2 (en) 2005-10-31 2012-05-22 The Board Of Trustees Of The Leland Stanford Junior University Cell-free synthesis of membrane bound polypeptides
US20100063258A1 (en) * 2006-06-28 2010-03-11 The Board Of Trustees Of The Leland Stanford Junior University Fusion protein constructs
EP2376619A4 (en) 2008-12-15 2012-07-04 Greenlight Biosciences Inc METHODS FOR CONTROLLING FLOWS IN METABOLIC PATHWAYS
JP2013526277A (ja) 2010-05-07 2013-06-24 グリーンライト バイオサイエンシーズ インコーポレーテッド 酵素リロケーションにより代謝経路のフラックスを制御する方法
EP2611922A1 (en) 2010-08-31 2013-07-10 Greenlight Biosciences, Inc. Methods for control of flux in metabolic pathways through protease manipulation
CN104093848A (zh) 2011-09-09 2014-10-08 绿光生物科学公司 碳青霉烯(carbapenem)的无细胞制备
CA2896079A1 (en) 2012-12-21 2014-06-26 Greenlight Biosciences, Inc. Cell-free system for converting methane into fuel and chemical compounds
JP2016520324A (ja) * 2013-06-05 2016-07-14 グリーンライト バイオサイエンシーズ インコーポレーテッドＧｒｅｅｎｌｉｇｈｔ Ｂｉｏｓｃｉｅｎｃｅｓ，Ｉｎｃ． 無細胞生合成系における代謝フラックスの制御
EP3460069B1 (en) 2013-08-05 2021-10-20 Greenlight Biosciences, Inc. Therapeutically active compounds and their methods of use
ES2963034T3 (es) 2013-08-19 2024-03-25 Dow Global Technologies Llc Método para producir un polímero a base de etileno en un procedimiento de polimerización usando un agente de autolimitación
WO2015190857A1 (ko) 2014-06-12 2015-12-17 충남대학교산학협력단 효소를 이용하여 ｐＨ를 조절하는 무세포 단백질 합성 방법
EP3277808A4 (en) 2015-03-30 2019-05-22 Greenlight Biosciences, Inc. ACELLULAR PRODUCTION OF RIBONUCLEIC ACID
CN105255818A (zh) * 2015-11-13 2016-01-20 肇庆大华农生物药品有限公司 一种改良的pk-15细胞传代培养方法及其应用
MY195729A (en) 2016-04-06 2023-02-07 Greenlight Biosciences Inc Cell-Free Production of Ribonucleic Acid
US11951165B2 (en) 2016-12-30 2024-04-09 Vaxcyte, Inc. Conjugated vaccine carrier proteins
AU2018205503A1 (en) 2017-01-06 2019-07-25 Greenlight Biosciences, Inc. Cell-free production of sugars
CN106834391A (zh) * 2017-01-18 2017-06-13 天津大学 一种基于人工多酶生化反应网络合成蛋白质的配方和方法
CN108396034A (zh) * 2017-02-06 2018-08-14 武汉臻智生物科技有限公司 一种提高无细胞体系蛋白质合成的方法
US11898187B2 (en) 2017-08-15 2024-02-13 Northwestern University Protein glycosylation sites by rapid expression and characterization of N-glycosyltransferases
MX2020003841A (es) 2017-10-11 2020-11-06 Greenlight Biosciences Inc Métodos y composiciones para la producción de nucleósido trifosfatos y ácidos ribonucleicos.
US11673921B2 (en) 2017-11-10 2023-06-13 Northwestern University Cell-free protein synthesis platform derived from cellular extracts of Vibrio natriegens
WO2019204346A1 (en) 2018-04-16 2019-10-24 Northwestern University METHODS FOR CO-ACTIVATING IN VITRO NON-STANDARD AMINO ACID (nsAA) INCORPORATION AND GLYCOSYLATION IN CRUDE CELLLYSATES
WO2020040840A2 (en) 2018-06-01 2020-02-27 Northwestern University Expanding the chemical substrates for genetic code reprogramming
AU2020328511A1 (en) 2019-08-09 2022-03-03 Nutcracker Therapeutics, Inc. Methods and apparatuses for manufacturing for removing material from a therapeutic composition

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US3990905A (en) * 1976-02-09 1976-11-09 Nalco Chemical Company Food process antifoam
US5547841A (en) * 1987-10-08 1996-08-20 The Mclean Hospital Corporation In vitro method for screening for drugs that inhibit production or degradation of human A4-amyloid
JPH04200390A (ja) 1990-11-30 1992-07-21 Shigeyuki Yokoyama 無細胞ポリペプチド合成系によるポリペプチドの製造方法
US5223412A (en) * 1991-02-21 1993-06-29 Genencor International, Inc. Cell-free and whole cell ice nucleators and process for their production
US5856174A (en) * 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
RU2148649C1 (ru) 1998-03-31 2000-05-10 Институт белка РАН Способ получения полипептидов в бесклеточной системе (варианты) и устройство для его осуществления
ES2217763T3 (es) * 1998-05-25 2004-11-01 Qiagen Gmbh Metodo para el aislamiento de adn plasmidico ccc.
EP1177311B1 (en) * 1999-03-17 2014-11-12 The Board Of Trustees Of The Leland Stanford Junior University In vitro macromolecule biosynthesis methods using exogenous amino acids and a novel atp regeneration system
US6337191B1 (en) * 1999-03-22 2002-01-08 The Board Of Trustees Of The Leland Stanford Junior University Vitro protein synthesis using glycolytic intermediates as an energy source
WO2003097829A1 (en) * 2002-05-22 2003-11-27 Riken Process for producing protein in cell-free protein synthesis system using thioredoxin fused protein expression vector
US20050123924A1 (en) * 2002-08-05 2005-06-09 Ayoub Rashtchian Compositions for in vitro amplification of nucleic acids
DK1685240T3 (da) * 2003-11-20 2015-02-16 Univ Leland Stanford Junior Forbedrede fremgangsmåder til in vitro-proteinsyntese

Also Published As

Publication number Publication date
CN1934276A (zh) 2007-03-21
CA2560504A1 (en) 2005-10-20
US8298759B2 (en) 2012-10-30
NO20064735L (no) 2006-10-19
JP4829215B2 (ja) 2011-12-07
AU2005230916B2 (en) 2012-03-08
KR101229849B1 (ko) 2013-02-05
WO2005098048A1 (en) 2005-10-20
AU2005230916A1 (en) 2005-10-20
EP1730313A4 (en) 2007-04-11
JP2007530042A (ja) 2007-11-01
EA200601748A1 (ru) 2007-04-27
EP1730313B1 (en) 2012-10-17
NZ549523A (en) 2009-10-30
ES2394443T3 (es) 2013-01-31
EP1730313A1 (en) 2006-12-13
KR20070011320A (ko) 2007-01-24
EA010837B1 (ru) 2008-12-30
NO331586B1 (no) 2012-01-30
CA2560504C (en) 2014-09-16
US20090042244A1 (en) 2009-02-12
MXPA06010918A (es) 2006-12-15

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