DE60029225T2 - Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese - Google Patents
Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese Download PDFInfo
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- DE60029225T2 DE60029225T2 DE60029225T DE60029225T DE60029225T2 DE 60029225 T2 DE60029225 T2 DE 60029225T2 DE 60029225 T DE60029225 T DE 60029225T DE 60029225 T DE60029225 T DE 60029225T DE 60029225 T2 DE60029225 T2 DE 60029225T2
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- nucleic acid
- oligonucleotide
- polymerase
- reverse transcriptase
- synthesis
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Classifications
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
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- A61P31/14—Antivirals for RNA viruses
- A61P31/18—Antivirals for RNA viruses for HIV
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- A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
- A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
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- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
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- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
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- Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
Applications Claiming Priority (3)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US14207299P | 1999-07-02 | 1999-07-02 | |
| US142072P | 1999-07-02 | ||
| PCT/US2000/018256 WO2001002559A1 (en) | 1999-07-02 | 2000-06-30 | Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| DE60029225D1 DE60029225D1 (de) | 2006-08-17 |
| DE60029225T2 true DE60029225T2 (de) | 2007-07-05 |
Family
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
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| DE60029225T Expired - Lifetime DE60029225T2 (de) | 1999-07-02 | 2000-06-30 | Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese |
Country Status (8)
| Country | Link |
|---|---|
| EP (1) | EP1196559B1 (https=) |
| JP (2) | JP2003504018A (https=) |
| AT (1) | ATE332371T1 (https=) |
| AU (1) | AU782555B2 (https=) |
| CA (1) | CA2377780A1 (https=) |
| DE (1) | DE60029225T2 (https=) |
| NZ (1) | NZ516894A (https=) |
| WO (1) | WO2001002559A1 (https=) |
Families Citing this family (13)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2002064833A1 (en) | 2001-02-15 | 2002-08-22 | Takara Bio Inc. | Method of detecting nucleotide polymorphism |
| EP2330194A3 (en) * | 2002-09-13 | 2011-10-12 | Replicor, Inc. | Non-sequence complementary antiviral oligonucleotides |
| EP1802774B1 (en) * | 2004-10-18 | 2012-01-11 | Brandeis University | Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification |
| WO2007038422A2 (en) | 2005-09-26 | 2007-04-05 | Allelogic Biosciences Corp. | Oligonucleotides as temperature-sensitive inhibitors for dna polymerases |
| EP2458011B1 (en) | 2005-10-03 | 2013-07-24 | Life Technologies Corporation | Compositions, methods, and kits for amplifying nucleic acids |
| US7781165B2 (en) | 2005-10-19 | 2010-08-24 | Roche Diagnostics Operations, Inc. | Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification |
| JPWO2012070618A1 (ja) | 2010-11-24 | 2014-05-19 | 株式会社カネカ | 増幅核酸検出方法及び検出デバイス |
| WO2013154898A1 (en) * | 2012-04-12 | 2013-10-17 | Ong, Jennifer | Synthetic nucleic acids for polymerization reactions |
| WO2013162026A1 (ja) * | 2012-04-27 | 2013-10-31 | 株式会社カネカ | 核酸の増幅方法、および、増幅核酸の検出方法 |
| US10392652B2 (en) | 2013-11-22 | 2019-08-27 | Kaneka Corporation | Micro RNA detection method using two primers to produce an amplified double stranded DNA fragment having a single stranded region at one end |
| JP7323534B2 (ja) * | 2018-01-05 | 2023-08-08 | ストラトス ゲノミクス インコーポレイテッド | 芳香族化合物による核酸重合の強化 |
| KR102275038B1 (ko) * | 2019-12-11 | 2021-07-08 | (주) 제노텍 | 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트 |
| JP2023531027A (ja) * | 2020-06-22 | 2023-07-20 | ▲蘇▼州新海生物科技股▲ふん▼有限公司 | 核酸リガンド、およびその使用 |
Family Cites Families (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5763173A (en) * | 1990-06-11 | 1998-06-09 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand inhibitors to DNA polymerases |
| DE69638318D1 (de) * | 1995-06-07 | 2011-02-17 | Gilead Sciences Inc | Nukleinsäureliganden, die an DNA-Polymerasen binden und diese inhibieren |
| US5760012A (en) * | 1996-05-01 | 1998-06-02 | Thomas Jefferson University | Methods and compounds for curing diseases caused by mutations |
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2000
- 2000-06-30 AU AU57850/00A patent/AU782555B2/en not_active Ceased
- 2000-06-30 EP EP00943373A patent/EP1196559B1/en not_active Expired - Lifetime
- 2000-06-30 CA CA002377780A patent/CA2377780A1/en not_active Abandoned
- 2000-06-30 NZ NZ516894A patent/NZ516894A/en unknown
- 2000-06-30 AT AT00943373T patent/ATE332371T1/de not_active IP Right Cessation
- 2000-06-30 DE DE60029225T patent/DE60029225T2/de not_active Expired - Lifetime
- 2000-06-30 JP JP2001508332A patent/JP2003504018A/ja active Pending
- 2000-06-30 WO PCT/US2000/018256 patent/WO2001002559A1/en not_active Ceased
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2010
- 2010-10-15 JP JP2010232252A patent/JP2011041572A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| JP2003504018A (ja) | 2003-02-04 |
| CA2377780A1 (en) | 2001-01-11 |
| AU782555B2 (en) | 2005-08-11 |
| AU5785000A (en) | 2001-01-22 |
| WO2001002559A1 (en) | 2001-01-11 |
| EP1196559B1 (en) | 2006-07-05 |
| WO2001002559A9 (en) | 2002-07-25 |
| JP2011041572A (ja) | 2011-03-03 |
| EP1196559A1 (en) | 2002-04-17 |
| NZ516894A (en) | 2004-06-25 |
| DE60029225D1 (de) | 2006-08-17 |
| ATE332371T1 (de) | 2006-07-15 |
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