NZ516894A - Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis - Google Patents
Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesisInfo
- Publication number
- NZ516894A NZ516894A NZ516894A NZ51689400A NZ516894A NZ 516894 A NZ516894 A NZ 516894A NZ 516894 A NZ516894 A NZ 516894A NZ 51689400 A NZ51689400 A NZ 51689400A NZ 516894 A NZ516894 A NZ 516894A
- Authority
- NZ
- New Zealand
- Prior art keywords
- nucleic acid
- oligonucleotide
- polymerase
- inhibitor
- synthesis
- Prior art date
Links
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
- C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
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- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
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- C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
- C12Q1/6844—Nucleic acid amplification reactions
- C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
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- Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
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| US14207299P | 1999-07-02 | 1999-07-02 | |
| PCT/US2000/018256 WO2001002559A1 (en) | 1999-07-02 | 2000-06-30 | Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis |
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| NZ516894A true NZ516894A (en) | 2004-06-25 |
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| NZ516894A NZ516894A (en) | 1999-07-02 | 2000-06-30 | Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis |
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| EP (1) | EP1196559B1 (https=) |
| JP (2) | JP2003504018A (https=) |
| AT (1) | ATE332371T1 (https=) |
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| CA (1) | CA2377780A1 (https=) |
| DE (1) | DE60029225T2 (https=) |
| NZ (1) | NZ516894A (https=) |
| WO (1) | WO2001002559A1 (https=) |
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| EP1367136A4 (en) | 2001-02-15 | 2005-01-12 | Takara Bio Inc | METHOD FOR DETECTING NUCLEOTIDE POLYMORPHISM |
| BR0314236A (pt) * | 2002-09-13 | 2005-08-09 | Replicor Inc | Formulação de oligonucleotìdeo, composição farmacêutica, kit, composto antiviral, preparação de oligonucleotìdeo e métodos para seleção de um oligonucleotìdeo antiviral para uso como um agente antiviral, para profilaxia ou tratamento de uma infecção viral em um paciente, para tratamento profilático de câncer causado por oncovìrus, para identificação de um composto que altera a ligação de um oligonucleotìdeo a pelo menos um componente viral, para purificação da ligação de oligonucleotìdeos a pelo menos um componente viral e para enriquecimento de oligonucleotìdeos a partir de um agrupamento de oligonucleotìdeos |
| ATE541055T1 (de) * | 2004-10-18 | 2012-01-15 | Univ Brandeis | Reagenzien und verfahren zur verbesserung der reproduzierbarkeit und zur reduzierung des mispriming bei der pcr-amplifikation |
| US8530194B2 (en) | 2005-09-26 | 2013-09-10 | Allelogic Biosciences Corporation | Oligonucleotides as temperature-sensitive inhibitors for DNA polymerases |
| US20070212704A1 (en) | 2005-10-03 | 2007-09-13 | Applera Corporation | Compositions, methods, and kits for amplifying nucleic acids |
| US7781165B2 (en) | 2005-10-19 | 2010-08-24 | Roche Diagnostics Operations, Inc. | Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification |
| SG190377A1 (en) | 2010-11-24 | 2013-06-28 | Kaneka Corp | Amplified nucleic acid detection method and detection device |
| WO2013154898A1 (en) * | 2012-04-12 | 2013-10-17 | Ong, Jennifer | Synthetic nucleic acids for polymerization reactions |
| JP6219816B2 (ja) * | 2012-04-27 | 2017-10-25 | 株式会社カネカ | 核酸の増幅方法、および、増幅核酸の検出方法 |
| WO2015076356A1 (ja) * | 2013-11-22 | 2015-05-28 | 株式会社カネカ | 短鎖ｒｎａの検出方法 |
| WO2019135975A1 (en) * | 2018-01-05 | 2019-07-11 | Stratos Genomics Inc. | Enhancement of nucleic acid polymerization by aromatic compounds |
| KR102275038B1 (ko) | 2019-12-11 | 2021-07-08 | (주) 제노텍 | 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트 |
| JP2023531027A (ja) * | 2020-06-22 | 2023-07-20 | ▲蘇▼州新海生物科技股▲ふん▼有限公司 | 核酸リガンド、およびその使用 |
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|---|---|---|---|---|
| US5763173A (en) * | 1990-06-11 | 1998-06-09 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligand inhibitors to DNA polymerases |
| US6020130A (en) * | 1995-06-07 | 2000-02-01 | Nexstar Pharmaceuticals, Inc. | Nucleic acid ligands that bind to and inhibit DNA polymerases |
| US5760012A (en) * | 1996-05-01 | 1998-06-02 | Thomas Jefferson University | Methods and compounds for curing diseases caused by mutations |
-
2000
- 2000-06-30 DE DE60029225T patent/DE60029225T2/de not_active Expired - Lifetime
- 2000-06-30 AU AU57850/00A patent/AU782555B2/en not_active Ceased
- 2000-06-30 WO PCT/US2000/018256 patent/WO2001002559A1/en not_active Ceased
- 2000-06-30 CA CA002377780A patent/CA2377780A1/en not_active Abandoned
- 2000-06-30 EP EP00943373A patent/EP1196559B1/en not_active Expired - Lifetime
- 2000-06-30 JP JP2001508332A patent/JP2003504018A/ja active Pending
- 2000-06-30 AT AT00943373T patent/ATE332371T1/de not_active IP Right Cessation
- 2000-06-30 NZ NZ516894A patent/NZ516894A/en unknown
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2010
- 2010-10-15 JP JP2010232252A patent/JP2011041572A/ja active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| DE60029225T2 (de) | 2007-07-05 |
| CA2377780A1 (en) | 2001-01-11 |
| WO2001002559A9 (en) | 2002-07-25 |
| EP1196559A1 (en) | 2002-04-17 |
| AU5785000A (en) | 2001-01-22 |
| DE60029225D1 (de) | 2006-08-17 |
| JP2011041572A (ja) | 2011-03-03 |
| AU782555B2 (en) | 2005-08-11 |
| WO2001002559A1 (en) | 2001-01-11 |
| EP1196559B1 (en) | 2006-07-05 |
| JP2003504018A (ja) | 2003-02-04 |
| ATE332371T1 (de) | 2006-07-15 |
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