ATE332371T1 - Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese - Google Patents

Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese

Info

Publication number
ATE332371T1
ATE332371T1 AT00943373T AT00943373T ATE332371T1 AT E332371 T1 ATE332371 T1 AT E332371T1 AT 00943373 T AT00943373 T AT 00943373T AT 00943373 T AT00943373 T AT 00943373T AT E332371 T1 ATE332371 T1 AT E332371T1
Authority
AT
Austria
Prior art keywords
nucleic acid
synthesis
relates
inhibitors
compositions
Prior art date
Application number
AT00943373T
Other languages
English (en)
Inventor
Mekbib Astatke
Deb Chatterjee
Gary Gerard
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of ATE332371T1 publication Critical patent/ATE332371T1/de

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • Physics & Mathematics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Biophysics (AREA)
  • Pharmacology & Pharmacy (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Virology (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Analytical Chemistry (AREA)
  • Oncology (AREA)
  • Communicable Diseases (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • AIDS & HIV (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
AT00943373T 1999-07-02 2000-06-30 Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese ATE332371T1 (de)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
US14207299P 1999-07-02 1999-07-02

Publications (1)

Publication Number Publication Date
ATE332371T1 true ATE332371T1 (de) 2006-07-15

Family

ID=22498453

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00943373T ATE332371T1 (de) 1999-07-02 2000-06-30 Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese

Country Status (8)

Country Link
EP (1) EP1196559B1 (de)
JP (2) JP2003504018A (de)
AT (1) ATE332371T1 (de)
AU (1) AU782555B2 (de)
CA (1) CA2377780A1 (de)
DE (1) DE60029225T2 (de)
NZ (1) NZ516894A (de)
WO (1) WO2001002559A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP1367136A4 (de) 2001-02-15 2005-01-12 Takara Bio Inc Verfahren zum nachweis von nukleotidpolymorphismus
EP1537208A1 (de) * 2002-09-13 2005-06-08 Replicor, Inc. Nicht-sequenzkomplementäre antivirale oligonukleotide
WO2006044995A1 (en) * 2004-10-18 2006-04-27 Brandeis University Reagents and methods for improving reproducibility and reducing mispriming in pcr amplification
WO2007038422A2 (en) 2005-09-26 2007-04-05 Allelogic Biosciences Corp. Oligonucleotides as temperature-sensitive inhibitors for dna polymerases
CN101321877B (zh) * 2005-10-03 2013-04-10 应用生物系统有限责任公司 用于扩增核酸的组合物、方法和试剂盒
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
WO2012070618A1 (ja) 2010-11-24 2012-05-31 株式会社カネカ 増幅核酸検出方法及び検出デバイス
EP2836603B1 (de) * 2012-04-12 2018-06-06 New England Biolabs, Inc. Synthetische nukleinsäuren für polymerisationsreaktionen
JP6219816B2 (ja) * 2012-04-27 2017-10-25 株式会社カネカ 核酸の増幅方法、および、増幅核酸の検出方法
JP6691380B2 (ja) * 2013-11-22 2020-04-28 株式会社カネカ 短鎖ｒｎａの検出方法
JP7323534B2 (ja) * 2018-01-05 2023-08-08 ストラトス ゲノミクス インコーポレイテッド 芳香族化合物による核酸重合の強化
KR102275038B1 (ko) 2019-12-11 2021-07-08 (주) 제노텍 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763173A (en) * 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
AU726844B2 (en) * 1995-06-07 2000-11-23 Nexstar Pharmaceuticals, Inc. Nucleic acid ligands that bind to and inhibit DNA polymerases
US5760012A (en) * 1996-05-01 1998-06-02 Thomas Jefferson University Methods and compounds for curing diseases caused by mutations

Also Published As

Publication number Publication date
DE60029225D1 (de) 2006-08-17
CA2377780A1 (en) 2001-01-11
NZ516894A (en) 2004-06-25
WO2001002559A1 (en) 2001-01-11
JP2003504018A (ja) 2003-02-04
AU5785000A (en) 2001-01-22
EP1196559A1 (de) 2002-04-17
EP1196559B1 (de) 2006-07-05
DE60029225T2 (de) 2007-07-05
WO2001002559A9 (en) 2002-07-25
JP2011041572A (ja) 2011-03-03
AU782555B2 (en) 2005-08-11

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