DE60029225D1 - Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese - Google Patents

Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese

Info

Publication number
DE60029225D1
DE60029225D1 DE60029225T DE60029225T DE60029225D1 DE 60029225 D1 DE60029225 D1 DE 60029225D1 DE 60029225 T DE60029225 T DE 60029225T DE 60029225 T DE60029225 T DE 60029225T DE 60029225 D1 DE60029225 D1 DE 60029225D1
Authority
DE
Germany
Prior art keywords
nucleic acid
synthesis
relates
inhibitors
compositions
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Expired - Lifetime
Application number
DE60029225T
Other languages
English (en)
Other versions
DE60029225T2 (de
Inventor
Mekbib Astatke
Deb Chatterjee
Gary Gerard
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Life Technologies Corp
Original Assignee
Invitrogen Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Invitrogen Corp filed Critical Invitrogen Corp
Application granted granted Critical
Publication of DE60029225D1 publication Critical patent/DE60029225D1/de
Publication of DE60029225T2 publication Critical patent/DE60029225T2/de
Anticipated expiration legal-status Critical
Expired - Lifetime legal-status Critical Current

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/115—Aptamers, i.e. nucleic acids binding a target molecule specifically and with high affinity without hybridising therewith ; Nucleic acids binding to non-nucleic acids, e.g. aptamers
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P31/00—Antiinfectives, i.e. antibiotics, antiseptics, chemotherapeutics
    • A61P31/12—Antivirals
    • A61P31/14—Antivirals for RNA viruses
    • A61P31/18—Antivirals for RNA viruses for HIV
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P43/00—Drugs for specific purposes, not provided for in groups A61P1/00-A61P41/00
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6848—Nucleic acid amplification reactions characterised by the means for preventing contamination or increasing the specificity or sensitivity of an amplification reaction
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Microbiology (AREA)
  • Veterinary Medicine (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Public Health (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Animal Behavior & Ethology (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Communicable Diseases (AREA)
  • Immunology (AREA)
  • Oncology (AREA)
  • Analytical Chemistry (AREA)
  • AIDS & HIV (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Pharmaceuticals Containing Other Organic And Inorganic Compounds (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)
DE60029225T 1999-07-02 2000-06-30 Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese Expired - Lifetime DE60029225T2 (de)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US14207299P 1999-07-02 1999-07-02
US142072P 1999-07-02
PCT/US2000/018256 WO2001002559A1 (en) 1999-07-02 2000-06-30 Compositions and methods for enhanced sensitivity and specificity of nucleic acid synthesis

Publications (2)

Publication Number Publication Date
DE60029225D1 true DE60029225D1 (de) 2006-08-17
DE60029225T2 DE60029225T2 (de) 2007-07-05

Family

ID=22498453

Family Applications (1)

Application Number Title Priority Date Filing Date
DE60029225T Expired - Lifetime DE60029225T2 (de) 1999-07-02 2000-06-30 Zusammensetzungen und verfahren zu höhere empfindlichkeit und spezifität von nukleinsäuresynthese

Country Status (8)

Country Link
EP (1) EP1196559B1 (de)
JP (2) JP2003504018A (de)
AT (1) ATE332371T1 (de)
AU (1) AU782555B2 (de)
CA (1) CA2377780A1 (de)
DE (1) DE60029225T2 (de)
NZ (1) NZ516894A (de)
WO (1) WO2001002559A1 (de)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2002064833A1 (fr) 2001-02-15 2002-08-22 Takara Bio Inc. Procede de detection de polymorphisme de nucleotide
JP2005538186A (ja) * 2002-09-13 2005-12-15 レプリコール インコーポレーティッド 非配列相補性の抗ウイルス性オリゴヌクレオチド
NZ554701A (en) * 2004-10-18 2010-03-26 Univ Brandeis Reagents and methods for improving reproducibility and reducing mispriming in PCR amplification
US8530194B2 (en) 2005-09-26 2013-09-10 Allelogic Biosciences Corporation Oligonucleotides as temperature-sensitive inhibitors for DNA polymerases
EP2458011B1 (de) * 2005-10-03 2013-07-24 Life Technologies Corporation Zusammensetzungen, Verfahren und Kits zur Amplifikation von Nukleinsäuren
US7781165B2 (en) 2005-10-19 2010-08-24 Roche Diagnostics Operations, Inc. Benzimidazolium compounds and salts of benzimidazolium compounds for nucleic acid amplification
CN103228785B (zh) 2010-11-24 2016-09-07 株式会社钟化 扩增核酸的检测方法和检测装置
EP2836603B1 (de) * 2012-04-12 2018-06-06 New England Biolabs, Inc. Synthetische nukleinsäuren für polymerisationsreaktionen
WO2013162026A1 (ja) * 2012-04-27 2013-10-31 株式会社カネカ 核酸の増幅方法、および、増幅核酸の検出方法
JP6691380B2 (ja) * 2013-11-22 2020-04-28 株式会社カネカ 短鎖ｒｎａの検出方法
JP7323534B2 (ja) * 2018-01-05 2023-08-08 ストラトス ゲノミクス インコーポレイテッド 芳香族化合物による核酸重合の強化
KR102275038B1 (ko) 2019-12-11 2021-07-08 (주) 제노텍 대립 유전자의 구분성을 높이는 pcr 방법 및 pcr 킷트

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5763173A (en) * 1990-06-11 1998-06-09 Nexstar Pharmaceuticals, Inc. Nucleic acid ligand inhibitors to DNA polymerases
EP2080813B1 (de) * 1995-06-07 2011-01-05 Gilead Sciences, Inc. Nukleinsäureliganden, die an DNA-Polymerasen binden und diese inhibieren
US5760012A (en) * 1996-05-01 1998-06-02 Thomas Jefferson University Methods and compounds for curing diseases caused by mutations

Also Published As

Publication number Publication date
WO2001002559A9 (en) 2002-07-25
NZ516894A (en) 2004-06-25
AU782555B2 (en) 2005-08-11
WO2001002559A1 (en) 2001-01-11
EP1196559B1 (de) 2006-07-05
AU5785000A (en) 2001-01-22
JP2003504018A (ja) 2003-02-04
JP2011041572A (ja) 2011-03-03
DE60029225T2 (de) 2007-07-05
ATE332371T1 (de) 2006-07-15
CA2377780A1 (en) 2001-01-11
EP1196559A1 (de) 2002-04-17

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Legal Events

Date Code Title Description
8364 No opposition during term of opposition
8327 Change in the person/name/address of the patent owner

Owner name: LIFE TECHNOLOGIES CORP. (N.D.GES.D. STAATES DE, US

8328 Change in the person/name/address of the agent

Representative=s name: GRAF VON STOSCH PATENTANWALTSGESELLSCHAFT MBH, 805

8380 Miscellaneous part iii

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8380 Miscellaneous part iii

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