DE60006685D1 - Rot-emittierende [8,9]benzophenoxazin-nukleinsäurefarbstoffe und verfahren zu deren verwendung - Google Patents
Rot-emittierende [8,9]benzophenoxazin-nukleinsäurefarbstoffe und verfahren zu deren verwendungInfo
- Publication number
- DE60006685D1 DE60006685D1 DE60006685T DE60006685T DE60006685D1 DE 60006685 D1 DE60006685 D1 DE 60006685D1 DE 60006685 T DE60006685 T DE 60006685T DE 60006685 T DE60006685 T DE 60006685T DE 60006685 D1 DE60006685 D1 DE 60006685D1
- Authority
- DE
- Germany
- Prior art keywords
- red
- nucleic acid
- benzophenoxazine
- emittering
- acid dyes
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Lifetime
Links
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- -1 [8,9] BENZOPHENOXAZINE NUCLEIC ACID Chemical class 0.000 title 1
- 239000000980 acid dye Substances 0.000 title 1
- 239000000975 dye Substances 0.000 abstract 2
- 150000007523 nucleic acids Chemical class 0.000 abstract 2
- VKQAFQHYTKYTON-UHFFFAOYSA-N 12h-naphtho[1,2-g][1,2]benzoxazine Chemical compound C1=CC=CC2=C3CC4=CC=NOC4=CC3=CC=C21 VKQAFQHYTKYTON-UHFFFAOYSA-N 0.000 abstract 1
- 238000003556 assay Methods 0.000 abstract 1
- 239000000499 gel Substances 0.000 abstract 1
- 239000012466 permeate Substances 0.000 abstract 1
- 238000010186 staining Methods 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N1/00—Sampling; Preparing specimens for investigation
- G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
- G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07D—HETEROCYCLIC COMPOUNDS
- C07D265/00—Heterocyclic compounds containing six-membered rings having one nitrogen atom and one oxygen atom as the only ring hetero atoms
- C07D265/28—1,4-Oxazines; Hydrogenated 1,4-oxazines
- C07D265/34—1,4-Oxazines; Hydrogenated 1,4-oxazines condensed with carbocyclic rings
-
- C—CHEMISTRY; METALLURGY
- C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
- C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
- C09B19/00—Oxazine dyes
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Engineering & Computer Science (AREA)
- Molecular Biology (AREA)
- Analytical Chemistry (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Immunology (AREA)
- Pathology (AREA)
- Physics & Mathematics (AREA)
- Investigating Or Analysing Biological Materials (AREA)
- Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
- Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
- Plural Heterocyclic Compounds (AREA)
- Coloring (AREA)
- Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Applications Claiming Priority (3)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US09/389,918 US6140500A (en) | 1999-09-03 | 1999-09-03 | Red-emitting [8,9]benzophenoxazine nucleic acid dyes and methods for their use |
US389918 | 1999-09-03 | ||
PCT/US2000/024057 WO2001018124A1 (en) | 1999-09-03 | 2000-09-01 | Red-emitting [8,9]benzophenoxazine nucleic acid dyes and methods for their use |
Publications (2)
Publication Number | Publication Date |
---|---|
DE60006685D1 true DE60006685D1 (de) | 2003-12-24 |
DE60006685T2 DE60006685T2 (de) | 2004-10-14 |
Family
ID=23540303
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
DE60006685T Expired - Lifetime DE60006685T2 (de) | 1999-09-03 | 2000-09-01 | Rot-emittierende [8,9]benzophenoxazin-nukleinsäurefarbstoffe und verfahren zu deren verwendung |
Country Status (8)
Country | Link |
---|---|
US (1) | US6140500A (de) |
EP (1) | EP1208160B1 (de) |
JP (1) | JP2003509528A (de) |
AT (1) | ATE254648T1 (de) |
AU (1) | AU753608B2 (de) |
CA (1) | CA2382593C (de) |
DE (1) | DE60006685T2 (de) |
WO (1) | WO2001018124A1 (de) |
Families Citing this family (55)
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US6465644B1 (en) | 2000-05-02 | 2002-10-15 | Applera Corporation | Sulfonated [8,9] benzophenoxazine dyes and the use of their labelled conjugates |
US7169922B2 (en) | 2000-08-04 | 2007-01-30 | Invitrogen Corporation | Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings |
AU7918501A (en) | 2000-08-04 | 2002-02-18 | Molecular Probes Inc | Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings |
EP1213328A1 (de) * | 2000-12-08 | 2002-06-12 | Evotec OAI AG | Oxazinderivate |
US7101999B2 (en) * | 2000-09-06 | 2006-09-05 | Evotec Ag | Oxazine derivatives |
DE60216468T2 (de) * | 2001-03-09 | 2007-09-27 | Boston Probes, Inc., Bedford | Kombinationsoligomere betreffende verfahren, kits und zusammensetzungen |
WO2003045310A2 (en) | 2001-11-21 | 2003-06-05 | Applera Corporation | Digital assay |
US20040137469A1 (en) | 2002-09-08 | 2004-07-15 | Casale Ralph A | Methods, compositions and libraries pertaining PNA dimer and PNA oligomer synthesis |
US7417726B2 (en) * | 2003-09-19 | 2008-08-26 | Applied Biosystems Inc. | Normalization of data using controls |
US20060029948A1 (en) * | 2003-09-19 | 2006-02-09 | Gary Lim | Sealing cover and dye compatibility selection |
US7570443B2 (en) | 2003-09-19 | 2009-08-04 | Applied Biosystems, Llc | Optical camera alignment |
DE602004018732D1 (de) * | 2003-11-20 | 2009-02-12 | Exiqon As | Löscher-Zusammensetzung mit Anthraquinon |
WO2005077125A2 (en) * | 2004-02-11 | 2005-08-25 | Applera Corporation | Methods and compositions for detecting nucleic acids |
US7473529B1 (en) * | 2004-03-31 | 2009-01-06 | Iowa State University Research Foundation, Inc. | System and method for detecting cells or components thereof |
US20050255485A1 (en) * | 2004-05-14 | 2005-11-17 | Livak Kenneth J | Detection of gene duplications |
WO2005118806A2 (en) * | 2004-05-28 | 2005-12-15 | Ambion, Inc. | METHODS AND COMPOSITIONS INVOLVING MicroRNA |
WO2006016978A1 (en) * | 2004-06-30 | 2006-02-16 | Applera Corporation | Analog probe complexes |
ES2534300T3 (es) | 2004-11-12 | 2015-04-21 | Asuragen, Inc. | Procedimientos y composiciones que implican miARN y moléculas inhibidoras de miARN |
US20060292586A1 (en) * | 2004-12-17 | 2006-12-28 | Schroth Gary P | ID-tag complexes, arrays, and methods of use thereof |
US20060166238A1 (en) * | 2004-12-22 | 2006-07-27 | Ramsing Niels B | Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same |
EP2813581B1 (de) | 2005-01-06 | 2018-06-27 | Applied Biosystems, LLC | Verwendung von Polypeptiden mit Nukleinsäurebindungsaktivität in Verfahren schneller Nukleinsäurenamplifikation |
CN101189520A (zh) * | 2005-05-03 | 2008-05-28 | 亚佩瑞股份有限公司 | 荧光检测系统和供其使用的染料组 |
US8362250B2 (en) | 2005-05-24 | 2013-01-29 | Enzo Biochem, Inc. | Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest |
US20070087360A1 (en) * | 2005-06-20 | 2007-04-19 | Boyd Victoria L | Methods and compositions for detecting nucleotides |
US20070059713A1 (en) * | 2005-09-09 | 2007-03-15 | Lee Jun E | SSB-DNA polymerase fusion proteins |
WO2007106690A2 (en) | 2006-03-15 | 2007-09-20 | Siemens Healthcare Diagnostics Inc. | Degenerate nucleobase analogs |
WO2007140015A2 (en) | 2006-05-26 | 2007-12-06 | Althea Technologies, Inc | Biochemical analysis of partitioned cells |
US20080131878A1 (en) * | 2006-12-05 | 2008-06-05 | Asuragen, Inc. | Compositions and Methods for the Detection of Small RNA |
AU2007333106A1 (en) * | 2006-12-08 | 2008-06-19 | Asuragen, Inc. | miR-20 regulated genes and pathways as targets for therapeutic intervention |
US20080274458A1 (en) * | 2007-05-01 | 2008-11-06 | Latham Gary J | Nucleic acid quantitation methods |
EP2198050A1 (de) | 2007-09-14 | 2010-06-23 | Asuragen, INC. | Mikrornas mit unterschiedlicher expression bei zervikalkarzinom und verwendungen davon |
US8071562B2 (en) | 2007-12-01 | 2011-12-06 | Mirna Therapeutics, Inc. | MiR-124 regulated genes and pathways as targets for therapeutic intervention |
WO2009137807A2 (en) | 2008-05-08 | 2009-11-12 | Asuragen, Inc. | Compositions and methods related to mirna modulation of neovascularization or angiogenesis |
US8715944B2 (en) * | 2008-09-08 | 2014-05-06 | Enzo Life Sciences, Inc. | Fluorochromes for organelle tracing and multi-color imaging |
US9250249B2 (en) | 2008-09-08 | 2016-02-02 | Enzo Biochem, Inc. | Autophagy and phospholipidosis pathway assays |
US9334281B2 (en) * | 2008-09-08 | 2016-05-10 | Enzo Life Sciences, Inc. | Fluorochromes for organelle tracing and multi-color imaging |
EP2432796B1 (de) | 2009-05-21 | 2016-08-17 | Siemens Healthcare Diagnostics Inc. | Universaltags mit nicht-natürlichen nukleobasen |
EP2634266B1 (de) | 2009-05-22 | 2015-10-14 | Asuragen, INC. | miRNA-Biomarker für Erkrankungen der Prostata |
EP2775300A3 (de) | 2009-08-28 | 2015-04-01 | Asuragen, INC. | miRNA-Biomarker für Lungenerkrankungen |
JP6081366B2 (ja) | 2010-10-29 | 2017-02-15 | アスラジェン, インコーポレイテッド | リピート配列を分析するためのmPCR法 |
CN103597093A (zh) | 2010-12-29 | 2014-02-19 | 生命技术公司 | 作为荧光参考标准品的ddao化合物 |
WO2013040251A2 (en) | 2011-09-13 | 2013-03-21 | Asurgen, Inc. | Methods and compositions involving mir-135b for distinguishing pancreatic cancer from benign pancreatic disease |
EP2929050A1 (de) | 2012-12-10 | 2015-10-14 | AdvanDx, Inc. | Verwendung von sonden zur massenspektrometrischen identifizierung von mikroorganismen oder zellen und relevanten erkrankungen von interesse |
CA2935789A1 (en) | 2014-01-05 | 2015-07-09 | Biomirna Holdings Ltd. | Lung cancer determinations using mirna |
US10619210B2 (en) | 2014-02-07 | 2020-04-14 | The Johns Hopkins University | Predicting response to epigenetic drug therapy |
CN107109474B (zh) | 2014-08-19 | 2021-03-19 | 豪夫迈·罗氏有限公司 | 用于核酸检测的方法和组合物 |
WO2016094330A2 (en) | 2014-12-08 | 2016-06-16 | 20/20 Genesystems, Inc | Methods and machine learning systems for predicting the liklihood or risk of having cancer |
WO2016177774A1 (en) | 2015-05-04 | 2016-11-10 | Academisch Medisch Centrum | Method of quantifying mirnas using normalization |
WO2017015543A1 (en) | 2015-07-23 | 2017-01-26 | Asuragen, Inc. | Methods, compositions, kits, and uses for analysis of nucleic acids comprising repeating a/t-rich segments |
WO2018045162A1 (en) | 2016-09-01 | 2018-03-08 | Biogen Ma Inc. | Biomarkers predictive of primary progressive multiple sclerosis and uses thereof |
CN107602498B (zh) * | 2017-07-26 | 2020-07-28 | 华南理工大学 | 一种荧光探针及其制备方法与在酪氨酸酶检测中的应用 |
EP3774747A1 (de) * | 2018-04-04 | 2021-02-17 | Universidade do Minho | Benzophenoxazinderivate zur diagnose von neoplasien oder infektionen |
WO2020090294A1 (ja) * | 2018-10-31 | 2020-05-07 | 日立化成株式会社 | イオン性化合物、有機エレクトロニクス材料、インク組成物、及び有機エレクトロニクス素子 |
EP3719144A1 (de) | 2019-04-05 | 2020-10-07 | Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) | Mir-151a-3p als universelle endogene steuerung zur exosomenladungsnormalisierung |
US20220267857A1 (en) | 2019-07-19 | 2022-08-25 | Fundación Para La Investigación Biomédikca Del Hospital Universitario La Paz (Fibhulp) | Method for determining the response to treatment of a patient affected by non-small cell lung carcinoma (nsclc) |
Family Cites Families (15)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
DE488945C (de) * | 1924-09-12 | 1930-01-25 | I G Farbenindustrie Akt Ges | Verfahren zur Darstellung von Aminoalkylaminosubstitutionsprodukten der Azin-, Oxazin- und Thiazinreihe |
US4386149A (en) * | 1978-02-28 | 1983-05-31 | Ciba-Geigy Ag | Process for the production of photographic images by dye diffusion transfer and photographic material suitable in this process |
US4237281A (en) * | 1978-10-13 | 1980-12-02 | Ciba-Geigy Aktiengesellschaft | Dyestuffs containing amino or imino groups |
US4789742A (en) * | 1984-08-27 | 1988-12-06 | Spyros Theodoropulos | Isomaleimide and isophthalimide derivatives of chromophors |
US4622395A (en) * | 1984-10-01 | 1986-11-11 | Minnesota Mining And Manufacturing Company | Phenoxazine and phenothiazine dyes and leuco forms thereof |
US4714763A (en) * | 1985-07-11 | 1987-12-22 | Viomedics Inc. | Novel oxazine-ureas and thiazine urea chromophors as fluorescent labels |
US4962197A (en) * | 1988-02-12 | 1990-10-09 | Rowland Institute For Science | Photo-inactivation of cancer cells |
US5783687A (en) * | 1990-03-14 | 1998-07-21 | The Regents Of The University Of California | Dyes designed for high sensitivity detection of double-stranded DNA |
DE59309805D1 (de) * | 1992-12-15 | 1999-11-04 | Ciba Sc Holding Ag | Oxazinfarbstoffe, Verfahren zu deren Herstellung und deren Verwendung |
US5410030A (en) * | 1993-04-05 | 1995-04-25 | Molecular Probes, Inc. | Dimers of unsymmetrical cyanine dyes containing pyridinium moieties |
US5436134A (en) * | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
US5565554A (en) * | 1994-07-29 | 1996-10-15 | The Regents Of The University Of California | Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores |
GB9602265D0 (en) * | 1996-02-05 | 1996-04-03 | Amersham Int Plc | Benzophenoxazine dyes |
ID17684A (id) * | 1996-02-08 | 1998-01-22 | Bpb Plc | Papan bersemen |
US5876946A (en) * | 1997-06-03 | 1999-03-02 | Pharmacopeia, Inc. | High-throughput assay |
-
1999
- 1999-09-03 US US09/389,918 patent/US6140500A/en not_active Expired - Lifetime
-
2000
- 2000-09-01 WO PCT/US2000/024057 patent/WO2001018124A1/en active IP Right Grant
- 2000-09-01 CA CA002382593A patent/CA2382593C/en not_active Expired - Fee Related
- 2000-09-01 EP EP00959749A patent/EP1208160B1/de not_active Expired - Lifetime
- 2000-09-01 DE DE60006685T patent/DE60006685T2/de not_active Expired - Lifetime
- 2000-09-01 JP JP2001522339A patent/JP2003509528A/ja not_active Ceased
- 2000-09-01 AT AT00959749T patent/ATE254648T1/de not_active IP Right Cessation
- 2000-09-01 AU AU71015/00A patent/AU753608B2/en not_active Ceased
Also Published As
Publication number | Publication date |
---|---|
CA2382593A1 (en) | 2001-03-15 |
JP2003509528A (ja) | 2003-03-11 |
CA2382593C (en) | 2008-04-08 |
US6140500A (en) | 2000-10-31 |
EP1208160B1 (de) | 2003-11-19 |
AU753608B2 (en) | 2002-10-24 |
DE60006685T2 (de) | 2004-10-14 |
WO2001018124A1 (en) | 2001-03-15 |
AU7101500A (en) | 2001-04-10 |
EP1208160A1 (de) | 2002-05-29 |
ATE254648T1 (de) | 2003-12-15 |
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Legal Events
Date | Code | Title | Description |
---|---|---|---|
8364 | No opposition during term of opposition | ||
8327 | Change in the person/name/address of the patent owner |
Owner name: APPLIED BIOSYSTEMS, LLC (N. D. GES. D. STAATES, US |
|
8380 | Miscellaneous part iii |
Free format text: PFANDRECHT |
|
8380 | Miscellaneous part iii |
Free format text: PFANDRECHT AUFGEHOBEN |