ATE254648T1 - Rot-emittierende (8,9)benzophenoxazin- nukleinsäurefarbstoffe und verfahren zu deren verwendung - Google Patents

Rot-emittierende (8,9)benzophenoxazin- nukleinsäurefarbstoffe und verfahren zu deren verwendung

Info

Publication number
ATE254648T1
ATE254648T1 AT00959749T AT00959749T ATE254648T1 AT E254648 T1 ATE254648 T1 AT E254648T1 AT 00959749 T AT00959749 T AT 00959749T AT 00959749 T AT00959749 T AT 00959749T AT E254648 T1 ATE254648 T1 AT E254648T1
Authority
AT
Austria
Prior art keywords
emitting
red
nucleic acid
benzophenoxazine
acid dyes
Prior art date
Application number
AT00959749T
Other languages
English (en)
Inventor
Xiongwui Yan
Sheri Miraglia
Pau Miau Yuan
Original Assignee
Applera Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Applera Corp filed Critical Applera Corp
Application granted granted Critical
Publication of ATE254648T1 publication Critical patent/ATE254648T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N1/00—Sampling; Preparing specimens for investigation
    • G01N1/28—Preparing specimens for investigation including physical details of (bio-)chemical methods covered elsewhere, e.g. G01N33/50, C12Q
    • G01N1/30—Staining; Impregnating ; Fixation; Dehydration; Multistep processes for preparing samples of tissue, cell or nucleic acid material and the like for analysis
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07D—HETEROCYCLIC COMPOUNDS
    • C07D265/00—Heterocyclic compounds containing six-membered rings having one nitrogen atom and one oxygen atom as the only ring hetero atoms
    • C07D265/28—1,4-Oxazines; Hydrogenated 1,4-oxazines
    • C07D265/34—1,4-Oxazines; Hydrogenated 1,4-oxazines condensed with carbocyclic rings
    • C—CHEMISTRY; METALLURGY
    • C09—DYES; PAINTS; POLISHES; NATURAL RESINS; ADHESIVES; COMPOSITIONS NOT OTHERWISE PROVIDED FOR; APPLICATIONS OF MATERIALS NOT OTHERWISE PROVIDED FOR
    • C09B—ORGANIC DYES OR CLOSELY-RELATED COMPOUNDS FOR PRODUCING DYES, e.g. PIGMENTS; MORDANTS; LAKES
    • C09B19/00—Oxazine dyes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Heterocyclic Carbon Compounds Containing A Hetero Ring Having Nitrogen And Oxygen As The Only Ring Hetero Atoms (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Nitrogen And Oxygen Or Sulfur-Condensed Heterocyclic Ring Systems (AREA)
  • Coloring (AREA)
  • Plural Heterocyclic Compounds (AREA)
AT00959749T 1999-09-03 2000-09-01 Rot-emittierende (8,9)benzophenoxazin- nukleinsäurefarbstoffe und verfahren zu deren verwendung ATE254648T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US09/389,918 US6140500A (en) 1999-09-03 1999-09-03 Red-emitting [8,9]benzophenoxazine nucleic acid dyes and methods for their use
PCT/US2000/024057 WO2001018124A1 (en) 1999-09-03 2000-09-01 Red-emitting [8,9]benzophenoxazine nucleic acid dyes and methods for their use

Publications (1)

Publication Number Publication Date
ATE254648T1 true ATE254648T1 (de) 2003-12-15

Family

ID=23540303

Family Applications (1)

Application Number Title Priority Date Filing Date
AT00959749T ATE254648T1 (de) 1999-09-03 2000-09-01 Rot-emittierende (8,9)benzophenoxazin- nukleinsäurefarbstoffe und verfahren zu deren verwendung

Country Status (8)

Country Link
US (1) US6140500A (de)
EP (1) EP1208160B1 (de)
JP (1) JP2003509528A (de)
AT (1) ATE254648T1 (de)
AU (1) AU753608B2 (de)
CA (1) CA2382593C (de)
DE (1) DE60006685T2 (de)
WO (1) WO2001018124A1 (de)

Families Citing this family (56)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6465644B1 (en) 2000-05-02 2002-10-15 Applera Corporation Sulfonated [8,9] benzophenoxazine dyes and the use of their labelled conjugates
US6716979B2 (en) 2000-08-04 2004-04-06 Molecular Probes, Inc. Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
US7169922B2 (en) * 2000-08-04 2007-01-30 Invitrogen Corporation Derivatives of 1,2-dihydro-7-hydroxyquinolines containing fused rings
US7101999B2 (en) * 2000-09-06 2006-09-05 Evotec Ag Oxazine derivatives
EP1213328A1 (de) * 2000-12-08 2002-06-12 Evotec OAI AG Oxazinderivate
JP4408628B2 (ja) * 2001-03-09 2010-02-03 ボストン プローブス，インコーポレイテッド 組み合わせオリゴマーならびにそれらの調製のためのライブラリーに適する、方法、キット、および組成物
CN100360722C (zh) 2001-11-21 2008-01-09 艾普勒拉公司 数字化分析
WO2004022578A2 (en) * 2002-09-08 2004-03-18 Applera Corporation Methods, compositions and libraries pertaining pna dimer and pna oligomer synthesis
US7570443B2 (en) 2003-09-19 2009-08-04 Applied Biosystems, Llc Optical camera alignment
US7417726B2 (en) * 2003-09-19 2008-08-26 Applied Biosystems Inc. Normalization of data using controls
US20060029948A1 (en) * 2003-09-19 2006-02-09 Gary Lim Sealing cover and dye compatibility selection
ES2319648T3 (es) * 2003-11-20 2009-05-11 Exiqon A/S Composicion atenuadora que comprende restos de antraquinona.
WO2005077125A2 (en) * 2004-02-11 2005-08-25 Applera Corporation Methods and compositions for detecting nucleic acids
US7473529B1 (en) * 2004-03-31 2009-01-06 Iowa State University Research Foundation, Inc. System and method for detecting cells or components thereof
US20050255485A1 (en) * 2004-05-14 2005-11-17 Livak Kenneth J Detection of gene duplications
EP2471924A1 (de) * 2004-05-28 2012-07-04 Asuragen, INC. Verfahren und Zusammensetzungen mit microRNA
WO2006016978A1 (en) * 2004-06-30 2006-02-16 Applera Corporation Analog probe complexes
CA2850323A1 (en) 2004-11-12 2006-12-28 Asuragen, Inc. Methods and compositions involving mirna and mirna inhibitor molecules
US20060292586A1 (en) * 2004-12-17 2006-12-28 Schroth Gary P ID-tag complexes, arrays, and methods of use thereof
US20060166238A1 (en) * 2004-12-22 2006-07-27 Ramsing Niels B Probes, libraries and kits for analysis of mixtures of nucleic acids and methods for constructing the same
EP1836319B1 (de) 2005-01-06 2012-09-19 Life Technologies Corporation Polypeptide mit nukleinsäurebindender aktivität sowie verfahren zur nukleinsäureamplifikation
EP2312317A3 (de) 2005-05-03 2011-06-08 Life Technologies Corporation Fluoreszentes Erkennungssystem und Farbstoffsatz zur Verwendung damit
US8362250B2 (en) 2005-05-24 2013-01-29 Enzo Biochem, Inc. Fluorescent dyes and compounds, methods and kits useful for identifying specific organelles and regions in cells of interest
US20070087360A1 (en) * 2005-06-20 2007-04-19 Boyd Victoria L Methods and compositions for detecting nucleotides
US20070059713A1 (en) 2005-09-09 2007-03-15 Lee Jun E SSB-DNA polymerase fusion proteins
WO2007106690A2 (en) 2006-03-15 2007-09-20 Siemens Healthcare Diagnostics Inc. Degenerate nucleobase analogs
EP2636755A1 (de) 2006-05-26 2013-09-11 AltheaDx Incorporated Biochemische Analyse partitionierter Zellen
US20080131878A1 (en) * 2006-12-05 2008-06-05 Asuragen, Inc. Compositions and Methods for the Detection of Small RNA
EP2104734A2 (de) * 2006-12-08 2009-09-30 Asuragen, INC. Mir-20-regulierte gene und pfade als ziele für therapeutische interventionen
US20080274458A1 (en) * 2007-05-01 2008-11-06 Latham Gary J Nucleic acid quantitation methods
WO2009036332A1 (en) 2007-09-14 2009-03-19 Asuragen, Inc. Micrornas differentially expressed in cervical cancer and uses thereof
US8071562B2 (en) 2007-12-01 2011-12-06 Mirna Therapeutics, Inc. MiR-124 regulated genes and pathways as targets for therapeutic intervention
US8258111B2 (en) 2008-05-08 2012-09-04 The Johns Hopkins University Compositions and methods related to miRNA modulation of neovascularization or angiogenesis
US8715944B2 (en) * 2008-09-08 2014-05-06 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
US9250249B2 (en) 2008-09-08 2016-02-02 Enzo Biochem, Inc. Autophagy and phospholipidosis pathway assays
US9334281B2 (en) * 2008-09-08 2016-05-10 Enzo Life Sciences, Inc. Fluorochromes for organelle tracing and multi-color imaging
US20120142004A1 (en) 2009-05-21 2012-06-07 Siemens Healthcare Diagnostics Inc. Universal Tags With Non-Natural Nucleobases
CA2762986C (en) 2009-05-22 2018-03-06 Asuragen, Inc. Mirna biomarkers of prostate disease
EP2470897A4 (de) 2009-08-28 2013-05-29 Asuragen Inc Mirna-biomarker für lungenerkrankungen
EP2633073B1 (de) 2010-10-29 2017-04-26 Asuragen, Inc. Mpcr verfahren zur analyse von wiederholungssequenzen
WO2012092403A1 (en) 2010-12-29 2012-07-05 Life Technologies Corporation Ddao compounds as fluorescent reference standards
US9644241B2 (en) 2011-09-13 2017-05-09 Interpace Diagnostics, Llc Methods and compositions involving miR-135B for distinguishing pancreatic cancer from benign pancreatic disease
EP2929050A1 (de) 2012-12-10 2015-10-14 AdvanDx, Inc. Verwendung von sonden zur massenspektrometrischen identifizierung von mikroorganismen oder zellen und relevanten erkrankungen von interesse
WO2015101653A1 (en) 2014-01-05 2015-07-09 Biomirna Holdings Ltd Lung cancer determinations using mirna ratios
WO2015120382A1 (en) 2014-02-07 2015-08-13 The Johns Hopkins University Predicting response to epigenetic drug therapy
US11421268B2 (en) 2014-08-19 2022-08-23 Roche Molecular Systems, Inc. Methods and compositions for nucleic acid detection
WO2016094330A2 (en) 2014-12-08 2016-06-16 20/20 Genesystems, Inc Methods and machine learning systems for predicting the liklihood or risk of having cancer
WO2016177774A1 (en) 2015-05-04 2016-11-10 Academisch Medisch Centrum Method of quantifying mirnas using normalization
AU2016297631B2 (en) 2015-07-23 2022-08-04 Asuragen, Inc. Methods, compositions, kits, and uses for analysis of nucleic acids comprising repeating A/T-rich segments
WO2018045162A1 (en) 2016-09-01 2018-03-08 Biogen Ma Inc. Biomarkers predictive of primary progressive multiple sclerosis and uses thereof
CN107602498B (zh) * 2017-07-26 2020-07-28 华南理工大学 一种荧光探针及其制备方法与在酪氨酸酶检测中的应用
WO2019193544A1 (en) * 2018-04-04 2019-10-10 Universidade Do Minho Benzophenoxazine derivatives for diagnosis of neoplasia or infection
WO2020090294A1 (ja) * 2018-10-31 2020-05-07 日立化成株式会社 イオン性化合物、有機エレクトロニクス材料、インク組成物、及び有機エレクトロニクス素子
EP3719144A1 (de) 2019-04-05 2020-10-07 Fundación para la Investigación Biomédica del Hospital Universitario de la Paz (FIBHULP) Mir-151a-3p als universelle endogene steuerung zur exosomenladungsnormalisierung
US20220267857A1 (en) 2019-07-19 2022-08-25 Fundación Para La Investigación Biomédikca Del Hospital Universitario La Paz (Fibhulp) Method for determining the response to treatment of a patient affected by non-small cell lung carcinoma (nsclc)
KR20230055951A (ko) 2021-10-15 2023-04-26 아주대학교산학협력단 miR-625-3p의 건선 중증도 진단용 바이오마커로서의 용도

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE488945C (de) * 1924-09-12 1930-01-25 I G Farbenindustrie Akt Ges Verfahren zur Darstellung von Aminoalkylaminosubstitutionsprodukten der Azin-, Oxazin- und Thiazinreihe
US4386149A (en) * 1978-02-28 1983-05-31 Ciba-Geigy Ag Process for the production of photographic images by dye diffusion transfer and photographic material suitable in this process
US4237281A (en) * 1978-10-13 1980-12-02 Ciba-Geigy Aktiengesellschaft Dyestuffs containing amino or imino groups
US4789742A (en) * 1984-08-27 1988-12-06 Spyros Theodoropulos Isomaleimide and isophthalimide derivatives of chromophors
US4622395A (en) * 1984-10-01 1986-11-11 Minnesota Mining And Manufacturing Company Phenoxazine and phenothiazine dyes and leuco forms thereof
US4714763A (en) * 1985-07-11 1987-12-22 Viomedics Inc. Novel oxazine-ureas and thiazine urea chromophors as fluorescent labels
US4962197A (en) * 1988-02-12 1990-10-09 Rowland Institute For Science Photo-inactivation of cancer cells
US5783687A (en) * 1990-03-14 1998-07-21 The Regents Of The University Of California Dyes designed for high sensitivity detection of double-stranded DNA
EP0603129B1 (de) * 1992-12-15 1999-09-29 Ciba SC Holding AG Oxazinfarbstoffe, Verfahren zu deren Herstellung und deren Verwendung
US5410030A (en) * 1993-04-05 1995-04-25 Molecular Probes, Inc. Dimers of unsymmetrical cyanine dyes containing pyridinium moieties
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5565554A (en) * 1994-07-29 1996-10-15 The Regents Of The University Of California Dimeric fluorescent energy transfer dyes comprising asymmetric cyanine azole-indolenine chromophores
GB9602265D0 (en) * 1996-02-05 1996-04-03 Amersham Int Plc Benzophenoxazine dyes
ID17684A (id) * 1996-02-08 1998-01-22 Bpb Plc Papan bersemen
US5876946A (en) * 1997-06-03 1999-03-02 Pharmacopeia, Inc. High-throughput assay

Also Published As

Publication number Publication date
EP1208160A1 (de) 2002-05-29
WO2001018124A1 (en) 2001-03-15
AU7101500A (en) 2001-04-10
AU753608B2 (en) 2002-10-24
CA2382593A1 (en) 2001-03-15
DE60006685D1 (de) 2003-12-24
DE60006685T2 (de) 2004-10-14
CA2382593C (en) 2008-04-08
JP2003509528A (ja) 2003-03-11
EP1208160B1 (de) 2003-11-19
US6140500A (en) 2000-10-31

Similar Documents

Publication Publication Date Title
ATE254648T1 (de) Rot-emittierende (8,9)benzophenoxazin- nukleinsäurefarbstoffe und verfahren zu deren verwendung
DE60005787D1 (de) Wasserlösliche rhodaminfarbstoffe und ihre konjugate
ATE409238T1 (de) Nachweis der hybridisierung von nukleinsäuren durch fluoreszenzpolarisation
Hilwig et al. pH-dependent fluorescence of DNA and RNA in cytologic staining with “33258 Hoechst”
DE69401717D1 (de) Verfahren zur Aktivierung von polyanionischen,fluoreszierenden Farbstoffen in schwach dielektrischen Medien mit quaternären Oniumverbindungen
ATE236442T1 (de) Fluoreszierende cyaninfarbstoffe
DE60238493D1 (de) Is von seltenen zellen verwendete markierte zellen
ATE97500T1 (de) Verfahren zur unterscheidung zwischen intakten und beschaedigten zellen in einer probe.
DE69939945D1 (de) Verfahren zur isolierung von stammzellen
WO2002077011A3 (en) Oxidation-reduction sensitive green fluorescent protein variants
ATE304706T1 (de) System zur messung des elektrischen potentials durch eine membrane
ATE280243T1 (de) Homogenes verfahren zum nachweis von doppelstrang-nukleinsäuren mittels fluoreszierender farbstoffe und dafür nützliche kits
ATE417897T1 (de) Sulfonierte 8,9 benzophenoxazinfarbstoffe und die verwendung ihrer markierten konjugate
DE60120742D1 (de) Montageverfahren unter verwendung von einer informationsanzeige
DE60115205D1 (de) Nitroreduktase reporter-gen assay mit fluoreszenz erhöhung
DE69729469D1 (de) Zellbasiertes festphasentest
ATE283486T1 (de) Differenz-nachweismethoden unter verwendung einer vielzahl von abgestimmten farbstoffen
BR9800254A (pt) Reagente de coloração para a determinação de células sanguíneas
DE3671641D1 (de) Wasserloesliche azoverbindungen, verfahren zu deren herstellung und ihre verwendung als farbstoffe.
DK1003458T3 (da) fprøvning af leverfunktion
ES2144604T3 (es) Inmunosubstraccion electroforetica capilar para clasificar y determinar las proteinas m.
DE69018674D1 (de) Faserreaktive asymmetrische Dioxazinverbindungen und deren Verwendung als faserreaktive Farbstoffe.
WO2001062965A3 (en) Differential expression screening method
ATE333519T1 (de) Electrochemisches verfahren zum nachweis von nukleinsäuren
DE3669523D1 (de) Wasserloesliche disazoverbindungen, verfahren zu deren herstellung und ihre verwendung als farbstoffe.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties