ATE97500T1 - Verfahren zur unterscheidung zwischen intakten und beschaedigten zellen in einer probe. - Google Patents
Verfahren zur unterscheidung zwischen intakten und beschaedigten zellen in einer probe.Info
- Publication number
- ATE97500T1 ATE97500T1 AT89304754T AT89304754T ATE97500T1 AT E97500 T1 ATE97500 T1 AT E97500T1 AT 89304754 T AT89304754 T AT 89304754T AT 89304754 T AT89304754 T AT 89304754T AT E97500 T1 ATE97500 T1 AT E97500T1
- Authority
- AT
- Austria
- Prior art keywords
- intact
- damaged cells
- discrimination
- sample
- sorted
- Prior art date
Links
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N15/1456—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
- G01N15/1459—Electro-optical investigation, e.g. flow cytometers without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
- G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
- G01N33/56966—Animal cells
- G01N33/56972—White blood cells
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N2015/1006—Investigating individual particles for cytology
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume, or surface-area of porous materials
- G01N15/10—Investigating individual particles
- G01N15/14—Electro-optical investigation, e.g. flow cytometers
- G01N2015/1477—Multiparameters
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
- G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
- G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
- G01N21/64—Fluorescence; Phosphorescence
- G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
- G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
- Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y10S436/00—Chemistry: analytical and immunological testing
- Y10S436/80—Fluorescent dyes, e.g. rhodamine
Applications Claiming Priority (2)
Application Number | Priority Date | Filing Date | Title |
---|---|---|---|
US07/206,454 US5057413A (en) | 1988-06-13 | 1988-06-13 | Method for discriminating between intact and damaged cells in a sample |
EP89304754A EP0347039B1 (de) | 1988-06-13 | 1989-05-10 | Verfahren zur Unterscheidung zwischen intakten und beschädigten Zellen in einer Probe |
Publications (1)
Publication Number | Publication Date |
---|---|
ATE97500T1 true ATE97500T1 (de) | 1993-12-15 |
Family
ID=22766462
Family Applications (1)
Application Number | Title | Priority Date | Filing Date |
---|---|---|---|
AT89304754T ATE97500T1 (de) | 1988-06-13 | 1989-05-10 | Verfahren zur unterscheidung zwischen intakten und beschaedigten zellen in einer probe. |
Country Status (6)
Country | Link |
---|---|
US (1) | US5057413A (de) |
EP (1) | EP0347039B1 (de) |
JP (1) | JP2620810B2 (de) |
AT (1) | ATE97500T1 (de) |
DE (1) | DE68910730T2 (de) |
ES (1) | ES2061987T3 (de) |
Families Citing this family (58)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
IE76732B1 (en) * | 1990-08-07 | 1997-11-05 | Becton Dickinson Co | One step test for absolute counts |
JP2941041B2 (ja) * | 1990-11-16 | 1999-08-25 | シスメックス株式会社 | フローサイトメトリーによる白血球の分類方法 |
JP3070968B2 (ja) * | 1991-05-14 | 2000-07-31 | シスメックス株式会社 | 尿中の細胞分析用試薬及び方法 |
US5610027A (en) * | 1992-10-30 | 1997-03-11 | Micro-Med, Inc. | Microphoto lysis-anlaysis process to measure cell characteristics |
US5955295A (en) * | 1992-10-30 | 1999-09-21 | Micro-Med, Inc. | Micro lysis-analysis process to measure cell characteristics and diagnose diseases |
US5532139A (en) * | 1992-10-30 | 1996-07-02 | Micro-Med, Inc. | Micro lysis-analysis process to measure cell characteristics and diagnose diseases |
WO1994016102A1 (en) * | 1993-01-06 | 1994-07-21 | Boehringer Mannheim Corporation | Fluorescent detection of nonviable cells |
EP0608987B1 (de) * | 1993-01-26 | 2001-10-10 | Becton, Dickinson and Company | Verfahren zum Nachweis von seltenen Ereignissen |
DE69434942T2 (de) * | 1993-02-25 | 2007-11-29 | Abbott Laboratories, Abbott Park | Mehrzweckreagenzsystem zur schnellen lysierung von vollblutproben |
US5445946A (en) * | 1993-04-13 | 1995-08-29 | Molecular Probes, Inc. | Intravacuolar stains for yeast and other fungi |
US5545535A (en) * | 1993-04-13 | 1996-08-13 | Molecular Probes, Inc. | Fluorescent assay for bacterial gram reaction |
US5436134A (en) * | 1993-04-13 | 1995-07-25 | Molecular Probes, Inc. | Cyclic-substituted unsymmetrical cyanine dyes |
US5534416A (en) * | 1993-04-13 | 1996-07-09 | Molecular Probes, Inc. | Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes |
US5437980A (en) * | 1993-05-17 | 1995-08-01 | Molecular Probes, Inc. | Phenanthridium dye staining of nucleic acids in living cells |
CA2160962C (en) * | 1994-10-20 | 2009-12-29 | Junya Inoue | Reagent for analyzing solid components in urine and method for analyzing solid components by employing the same |
US5879900A (en) * | 1994-12-15 | 1999-03-09 | Abbott Laboratories | Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differentials |
US6329158B1 (en) * | 1995-09-15 | 2001-12-11 | Becton Dickinson And Company | Use of dimly fluorescing nucleic acid dyes in the identification of nucleated cells |
ATE472725T1 (de) * | 1995-12-15 | 2010-07-15 | Abbott Lab | Verfahren für gleichzeitige analyse von zelllebensfähigkeit, nukleierten roten blutkörperchen und leukozytendifferential |
CA2279574C (en) | 1997-01-31 | 2007-07-24 | The Horticulture & Food Research Institute Of New Zealand Ltd. | Optical apparatus |
US6149867A (en) | 1997-12-31 | 2000-11-21 | Xy, Inc. | Sheath fluids and collection systems for sex-specific cytometer sorting of sperm |
US6911313B2 (en) * | 1998-02-06 | 2005-06-28 | Sysmex Corporation | Process for discriminating and counting erythroblasts |
FR2782730B1 (fr) * | 1998-08-25 | 2002-05-17 | Biocom Sa | Procede de separation cellulaire pour l'isolation de cellules pathogeniques, notamment cancereuses rares, equipement et reactif pour la mise en oeuvre du procede et application du procede |
JP2002537557A (ja) * | 1999-02-19 | 2002-11-05 | アイデックス ラボラトリーズ インコーポレイテッド | 高開口数流れ血球計算器及びその使用方法 |
US6787302B2 (en) * | 1999-10-25 | 2004-09-07 | Genprime, Inc. | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
US6673568B1 (en) | 1999-10-25 | 2004-01-06 | Genprime, Inc. | Method and apparatus for prokaryotic and eukaryotic cell quantitation |
US7208265B1 (en) | 1999-11-24 | 2007-04-24 | Xy, Inc. | Method of cryopreserving selected sperm cells |
AU2610101A (en) | 2000-01-06 | 2001-07-16 | Caliper Technologies Corporation | Methods and systems for monitoring intracellular binding reactions |
US7713687B2 (en) | 2000-11-29 | 2010-05-11 | Xy, Inc. | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
CA2468772C (en) | 2000-11-29 | 2013-10-29 | George E. Seidel | System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations |
US6793642B2 (en) * | 2001-05-07 | 2004-09-21 | Biomed Solutions, Llc | Flow cytometer |
US6403378B1 (en) * | 2001-04-26 | 2002-06-11 | Guava Technologies, Inc. | Cell viability assay reagent |
WO2002092161A1 (en) * | 2001-05-10 | 2002-11-21 | Biophan, Llc | Miniaturized particle analyzer |
NZ538462A (en) | 2002-08-01 | 2008-06-30 | Xy Inc | Low pressure sperm cell separation system |
US8486618B2 (en) | 2002-08-01 | 2013-07-16 | Xy, Llc | Heterogeneous inseminate system |
US7855078B2 (en) | 2002-08-15 | 2010-12-21 | Xy, Llc | High resolution flow cytometer |
US7169548B2 (en) | 2002-09-13 | 2007-01-30 | Xy, Inc. | Sperm cell processing and preservation systems |
EP2308416B1 (de) | 2003-03-28 | 2015-01-07 | Inguran, LLC | Vorrichtung und Verfahren zur Bereitstellung von geschlechtssortierten Tierspermien |
AU2004242121B2 (en) | 2003-05-15 | 2010-06-24 | Xy, Llc. | Efficient haploid cell sorting for flow cytometer systems |
US7776529B2 (en) | 2003-12-05 | 2010-08-17 | Life Technologies Corporation | Methine-substituted cyanine dye compounds |
US7892725B2 (en) | 2004-03-29 | 2011-02-22 | Inguran, Llc | Process for storing a sperm dispersion |
AR049732A1 (es) | 2004-07-22 | 2006-08-30 | Monsanto Co | Proceso para enriquecer una poblacion de celulas de esperma |
JP5306811B2 (ja) | 2005-05-11 | 2013-10-02 | ライフ テクノロジーズ コーポレーション | 2本鎖dnaへの高い選択性を有する蛍光化学物質及びそれらの使用 |
EP2232243B1 (de) | 2007-12-20 | 2015-12-16 | Nederlandse Organisatie voor toegepast- natuurwetenschappelijk onderzoek TNO | Echtzeitverfahren zum nachweis lebensfähiger mikroorganismen |
US8710836B2 (en) * | 2008-12-10 | 2014-04-29 | Nanomr, Inc. | NMR, instrumentation, and flow meter/controller continuously detecting MR signals, from continuously flowing sample material |
GB201005939D0 (en) | 2010-04-09 | 2010-05-26 | Biostatus Ltd | Method of analysing a cell or other biological material containing nucleic acid |
US20110262989A1 (en) | 2010-04-21 | 2011-10-27 | Nanomr, Inc. | Isolating a target analyte from a body fluid |
US8841104B2 (en) | 2010-04-21 | 2014-09-23 | Nanomr, Inc. | Methods for isolating a target analyte from a heterogeneous sample |
US9428547B2 (en) | 2010-04-21 | 2016-08-30 | Dna Electronics, Inc. | Compositions for isolating a target analyte from a heterogeneous sample |
US9476812B2 (en) | 2010-04-21 | 2016-10-25 | Dna Electronics, Inc. | Methods for isolating a target analyte from a heterogeneous sample |
US9434940B2 (en) | 2012-12-19 | 2016-09-06 | Dna Electronics, Inc. | Methods for universal target capture |
US9599610B2 (en) | 2012-12-19 | 2017-03-21 | Dnae Group Holdings Limited | Target capture system |
US10000557B2 (en) | 2012-12-19 | 2018-06-19 | Dnae Group Holdings Limited | Methods for raising antibodies |
US9804069B2 (en) | 2012-12-19 | 2017-10-31 | Dnae Group Holdings Limited | Methods for degrading nucleic acid |
US9551704B2 (en) | 2012-12-19 | 2017-01-24 | Dna Electronics, Inc. | Target detection |
US9995742B2 (en) | 2012-12-19 | 2018-06-12 | Dnae Group Holdings Limited | Sample entry |
EP3165899A1 (de) * | 2015-11-04 | 2017-05-10 | Siemens Healthcare Diagnostics GmbH | Verfahren zum nachweis von neutrophilen extrazellulären fallen |
WO2020142785A1 (en) * | 2019-01-04 | 2020-07-09 | Gennext Technologies, Inc. | In vivo radical dosimetry and in vivo hydroxyl radical protein foot-printing |
CA3213163A1 (en) | 2021-03-25 | 2022-09-29 | Iovance Biotherapeutics, Inc. | Methods and compositions for t-cell coculture potency assays and use with cell therapy products |
Family Cites Families (14)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US3826364A (en) * | 1972-05-22 | 1974-07-30 | Univ Leland Stanford Junior | Particle sorting method and apparatus |
US4094745A (en) * | 1973-06-22 | 1978-06-13 | John Scholefield | Method of staining microscopic organisms |
US4284412A (en) * | 1979-07-13 | 1981-08-18 | Ortho Diagnostics, Inc. | Method and apparatus for automated identification and enumeration of specified blood cell subclasses |
US4544546A (en) * | 1980-04-21 | 1985-10-01 | Abbott Laboratories | Fluorescent nucleic acid stains |
US4520110A (en) * | 1981-10-06 | 1985-05-28 | The Board Of Trustees Of The Leland Stanford Junior University | Fluorescent immunoassay employing a phycobiliprotein labeled ligand or receptor |
JPS59184862A (ja) * | 1983-04-05 | 1984-10-20 | ベクトン・ディッキンソン・アンド・カンパニ− | 試料内細胞の複数副次集団を識別する方法と装置 |
US4585736A (en) * | 1983-10-18 | 1986-04-29 | The United States Of America As Represented By The United States Department Of Energy | Flow cytometric measurement of total DNA and incorporated halodeoxyuridine |
CA1254134A (en) * | 1984-03-28 | 1989-05-16 | Mount Sinai School Of Medicine Of The City University Of New York | Specific binding flow cytometry method |
US4661913A (en) * | 1984-09-11 | 1987-04-28 | Becton, Dickinson And Company | Apparatus and method for the detection and classification of articles using flow cytometry techniques |
US4727020A (en) * | 1985-02-25 | 1988-02-23 | Becton, Dickinson And Company | Method for analysis of subpopulations of blood cells |
JPH0663973B2 (ja) * | 1985-08-07 | 1994-08-22 | 東ソー株式会社 | 免疫反応測定に用いる蛍光検出装置 |
CA1279008C (en) * | 1985-10-11 | 1991-01-15 | Smith Kline & French Canada Ltd. | Methods and reagents for performing subset analysis |
ZA867698B (en) * | 1985-10-11 | 1987-07-29 | Smithkline Beckman Corp | Methods and reagents for performing subset analysis |
US5016283A (en) * | 1985-11-04 | 1991-05-14 | Cell Analysis Systems, Inc. | Methods and apparatus for immunoploidy analysis |
-
1988
- 1988-06-13 US US07/206,454 patent/US5057413A/en not_active Expired - Lifetime
-
1989
- 1989-05-10 ES ES89304754T patent/ES2061987T3/es not_active Expired - Lifetime
- 1989-05-10 EP EP89304754A patent/EP0347039B1/de not_active Expired - Lifetime
- 1989-05-10 AT AT89304754T patent/ATE97500T1/de not_active IP Right Cessation
- 1989-05-10 DE DE68910730T patent/DE68910730T2/de not_active Expired - Fee Related
- 1989-06-13 JP JP1150348A patent/JP2620810B2/ja not_active Expired - Lifetime
Also Published As
Publication number | Publication date |
---|---|
JP2620810B2 (ja) | 1997-06-18 |
DE68910730D1 (de) | 1993-12-23 |
US5057413A (en) | 1991-10-15 |
DE68910730T2 (de) | 1994-05-19 |
EP0347039A2 (de) | 1989-12-20 |
EP0347039A3 (en) | 1990-10-24 |
JPH02103464A (ja) | 1990-04-16 |
ES2061987T3 (es) | 1994-12-16 |
EP0347039B1 (de) | 1993-11-18 |
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Legal Events
Date | Code | Title | Description |
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RER | Ceased as to paragraph 5 lit. 3 law introducing patent treaties |