ATE97500T1 - Verfahren zur unterscheidung zwischen intakten und beschaedigten zellen in einer probe. - Google Patents

Verfahren zur unterscheidung zwischen intakten und beschaedigten zellen in einer probe.

Info

Publication number
ATE97500T1
ATE97500T1 AT89304754T AT89304754T ATE97500T1 AT E97500 T1 ATE97500 T1 AT E97500T1 AT 89304754 T AT89304754 T AT 89304754T AT 89304754 T AT89304754 T AT 89304754T AT E97500 T1 ATE97500 T1 AT E97500T1
Authority
AT
Austria
Prior art keywords
intact
damaged cells
discrimination
sample
sorted
Prior art date
Application number
AT89304754T
Other languages
English (en)
Inventor
Leon Terstappen
R Michael Loken
Virendra O Shah
Original Assignee
Becton Dickinson Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Becton Dickinson Co filed Critical Becton Dickinson Co
Application granted granted Critical
Publication of ATE97500T1 publication Critical patent/ATE97500T1/de

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56966—Animal cells
    • G01N33/56972—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1402—Data analysis by thresholding or gating operations performed on the acquired signals or stored data
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1477—Multiparameters
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N21/00—Investigating or analysing materials by the use of optical means, i.e. using sub-millimetre waves, infrared, visible or ultraviolet light
    • G01N21/62—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light
    • G01N21/63—Systems in which the material investigated is excited whereby it emits light or causes a change in wavelength of the incident light optically excited
    • G01N21/64—Fluorescence; Phosphorescence
    • G01N21/6428—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes"
    • G01N2021/6439—Measuring fluorescence of fluorescent products of reactions or of fluorochrome labelled reactive substances, e.g. measuring quenching effects, using measuring "optrodes" with indicators, stains, dyes, tags, labels, marks
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Immunology (AREA)
  • Physics & Mathematics (AREA)
  • Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Analytical Chemistry (AREA)
  • Hematology (AREA)
  • Zoology (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Cell Biology (AREA)
  • Molecular Biology (AREA)
  • Organic Chemistry (AREA)
  • Urology & Nephrology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Wood Science & Technology (AREA)
  • Optics & Photonics (AREA)
  • Virology (AREA)
  • General Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biophysics (AREA)
  • Dispersion Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
AT89304754T 1988-06-13 1989-05-10 Verfahren zur unterscheidung zwischen intakten und beschaedigten zellen in einer probe. ATE97500T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US07/206,454 US5057413A (en) 1988-06-13 1988-06-13 Method for discriminating between intact and damaged cells in a sample
EP89304754A EP0347039B1 (de) 1988-06-13 1989-05-10 Verfahren zur Unterscheidung zwischen intakten und beschädigten Zellen in einer Probe

Publications (1)

Publication Number Publication Date
ATE97500T1 true ATE97500T1 (de) 1993-12-15

Family

ID=22766462

Family Applications (1)

Application Number Title Priority Date Filing Date
AT89304754T ATE97500T1 (de) 1988-06-13 1989-05-10 Verfahren zur unterscheidung zwischen intakten und beschaedigten zellen in einer probe.

Country Status (6)

Country Link
US (1) US5057413A (de)
EP (1) EP0347039B1 (de)
JP (1) JP2620810B2 (de)
AT (1) ATE97500T1 (de)
DE (1) DE68910730T2 (de)
ES (1) ES2061987T3 (de)

Families Citing this family (63)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
IE76732B1 (en) * 1990-08-07 1997-11-05 Becton Dickinson Co One step test for absolute counts
JP2941041B2 (ja) * 1990-11-16 1999-08-25 シスメックス株式会社 フローサイトメトリーによる白血球の分類方法
JP3070968B2 (ja) * 1991-05-14 2000-07-31 シスメックス株式会社 尿中の細胞分析用試薬及び方法
US5610027A (en) * 1992-10-30 1997-03-11 Micro-Med, Inc. Microphoto lysis-anlaysis process to measure cell characteristics
US5955295A (en) * 1992-10-30 1999-09-21 Micro-Med, Inc. Micro lysis-analysis process to measure cell characteristics and diagnose diseases
US5532139A (en) * 1992-10-30 1996-07-02 Micro-Med, Inc. Micro lysis-analysis process to measure cell characteristics and diagnose diseases
CA2153257A1 (en) * 1993-01-06 1994-07-21 Terry J. Fetterhoff Fluorescent detection of nonviable cells
DE69428552T2 (de) * 1993-01-26 2002-04-11 Becton Dickinson And Co., Franklin Lakes Verfahren zum Nachweis von seltenen Ereignissen
DE69434942T2 (de) * 1993-02-25 2007-11-29 Abbott Laboratories, Abbott Park Mehrzweckreagenzsystem zur schnellen lysierung von vollblutproben
US5545535A (en) * 1993-04-13 1996-08-13 Molecular Probes, Inc. Fluorescent assay for bacterial gram reaction
US5436134A (en) * 1993-04-13 1995-07-25 Molecular Probes, Inc. Cyclic-substituted unsymmetrical cyanine dyes
US5445946A (en) * 1993-04-13 1995-08-29 Molecular Probes, Inc. Intravacuolar stains for yeast and other fungi
US5534416A (en) * 1993-04-13 1996-07-09 Molecular Probes, Inc. Fluorescent viability assay using cyclic-substituted unsymmetrical cyanine dyes
US5437980A (en) * 1993-05-17 1995-08-01 Molecular Probes, Inc. Phenanthridium dye staining of nucleic acids in living cells
CA2160962C (en) * 1994-10-20 2009-12-29 Junya Inoue Reagent for analyzing solid components in urine and method for analyzing solid components by employing the same
US5879900A (en) * 1994-12-15 1999-03-09 Abbott Laboratories Method for simultaneous analysis of cell viability, nucleated red blood cells and white blood cell differentials
US6329158B1 (en) * 1995-09-15 2001-12-11 Becton Dickinson And Company Use of dimly fluorescing nucleic acid dyes in the identification of nucleated cells
ES2347110T3 (es) * 1995-12-15 2010-10-25 Abbott Laboratories Metodo para el analisis simultaneo de viabilidad celular, diferencial0de globulos blancos.
JP4323571B2 (ja) 1997-01-31 2009-09-02 エックスワイ， インコーポレイテッド 光学装置
US6149867A (en) 1997-12-31 2000-11-21 Xy, Inc. Sheath fluids and collection systems for sex-specific cytometer sorting of sperm
US6071689A (en) 1997-12-31 2000-06-06 Xy, Inc. System for improving yield of sexed embryos in mammals
US6911313B2 (en) * 1998-02-06 2005-06-28 Sysmex Corporation Process for discriminating and counting erythroblasts
FR2782730B1 (fr) * 1998-08-25 2002-05-17 Biocom Sa Procede de separation cellulaire pour l'isolation de cellules pathogeniques, notamment cancereuses rares, equipement et reactif pour la mise en oeuvre du procede et application du procede
AU768616C (en) * 1999-02-19 2004-12-16 Idexx Laboratories, Inc. High numerical aperture flow cytometer and method of using same
US6673568B1 (en) 1999-10-25 2004-01-06 Genprime, Inc. Method and apparatus for prokaryotic and eukaryotic cell quantitation
US6787302B2 (en) * 1999-10-25 2004-09-07 Genprime, Inc. Method and apparatus for prokaryotic and eukaryotic cell quantitation
US7208265B1 (en) 1999-11-24 2007-04-24 Xy, Inc. Method of cryopreserving selected sperm cells
EP1244810B1 (de) * 2000-01-06 2008-02-20 Caliper Life Sciences, Inc. Methoden und syteme zur überwachung intrazellulärer bindereaktionen
AU2002220018A1 (en) 2000-11-29 2002-06-11 Colorado State University System for in-vitro fertilization with spermatozoa separated into x-chromosome and y-chromosome bearing populations
US7713687B2 (en) 2000-11-29 2010-05-11 Xy, Inc. System to separate frozen-thawed spermatozoa into x-chromosome bearing and y-chromosome bearing populations
US6793642B2 (en) * 2001-05-07 2004-09-21 Biomed Solutions, Llc Flow cytometer
US6403378B1 (en) * 2001-04-26 2002-06-11 Guava Technologies, Inc. Cell viability assay reagent
WO2002092161A1 (en) * 2001-05-10 2002-11-21 Biophan, Llc Miniaturized particle analyzer
US8486618B2 (en) 2002-08-01 2013-07-16 Xy, Llc Heterogeneous inseminate system
WO2004012837A2 (en) 2002-08-01 2004-02-12 Xy, Inc. Low pressure sperm cell separation system
WO2004017041A2 (en) 2002-08-15 2004-02-26 Xy, Inc. High resolution flow cytometer
US7169548B2 (en) 2002-09-13 2007-01-30 Xy, Inc. Sperm cell processing and preservation systems
DK2305172T3 (en) 2003-03-28 2016-08-22 Inguran Llc DEVICE AND PROCEDURES TO PROVIDE SEXUAL ANIMAL SEED
ES2541121T3 (es) 2003-05-15 2015-07-16 Xy, Llc Clasificación eficiente de células haploides por sistemas de citometría de flujo
US7776529B2 (en) 2003-12-05 2010-08-17 Life Technologies Corporation Methine-substituted cyanine dye compounds
BRPI0509485A (pt) 2004-03-29 2007-09-11 Monsanto Technology Llc suspensões de esperma para uso em inseminação
MX2007000888A (es) 2004-07-22 2007-04-02 Monsanto Technology Llc Procedimiento para enriquecer una poblacion de celulas de esperma.
WO2006124816A1 (en) 2005-05-11 2006-11-23 Molecular Probes, Inc. Fluorescent chemical compounds having high selectivity for double stranded dna, and methods for their use
US8986946B2 (en) 2007-12-20 2015-03-24 Nederlandse Organisatie Voor Toegepast-Natuurwetenschappelijk Onderzoek Tno Real-time method for the detection of viable micro-organisms
WO2010068812A1 (en) * 2008-12-10 2010-06-17 Abqmr, Inc. Nuclear magnetic resonance apparatus, methods and associated technology
GB201005939D0 (en) * 2010-04-09 2010-05-26 Biostatus Ltd Method of analysing a cell or other biological material containing nucleic acid
US9428547B2 (en) 2010-04-21 2016-08-30 Dna Electronics, Inc. Compositions for isolating a target analyte from a heterogeneous sample
US9476812B2 (en) 2010-04-21 2016-10-25 Dna Electronics, Inc. Methods for isolating a target analyte from a heterogeneous sample
US8841104B2 (en) 2010-04-21 2014-09-23 Nanomr, Inc. Methods for isolating a target analyte from a heterogeneous sample
US20110262989A1 (en) 2010-04-21 2011-10-27 Nanomr, Inc. Isolating a target analyte from a body fluid
US9434940B2 (en) 2012-12-19 2016-09-06 Dna Electronics, Inc. Methods for universal target capture
US10000557B2 (en) 2012-12-19 2018-06-19 Dnae Group Holdings Limited Methods for raising antibodies
US9551704B2 (en) 2012-12-19 2017-01-24 Dna Electronics, Inc. Target detection
US9599610B2 (en) 2012-12-19 2017-03-21 Dnae Group Holdings Limited Target capture system
US9804069B2 (en) 2012-12-19 2017-10-31 Dnae Group Holdings Limited Methods for degrading nucleic acid
US9995742B2 (en) 2012-12-19 2018-06-12 Dnae Group Holdings Limited Sample entry
EP3165899A1 (de) * 2015-11-04 2017-05-10 Siemens Healthcare Diagnostics GmbH Verfahren zum nachweis von neutrophilen extrazellulären fallen
US12013400B2 (en) 2017-05-26 2024-06-18 Gennext Technologies, Inc. Radical dosimetry methods for in vivo hydroxyl radical protein foot-printing
US12016688B2 (en) 2017-05-26 2024-06-25 Gennext Technologies, Inc. In vivo radical dosimetry and in vivo hydroxyl radical protein foot-printing
US12263477B2 (en) 2018-10-18 2025-04-01 Gennext Technologies, Inc. Opto-fluidic array for radical protein foot-printing
EP3906395B1 (de) * 2019-01-04 2024-12-04 GenNext Technologies, Inc. In-vivo-radikaldosimetrie und in-vivo-hydroxylradikal-protein-foot-printing
JP7574195B2 (ja) * 2019-01-04 2024-10-28 ジェネクスト テクノロジーズ インコーポレイテッド 生体内ラジカル線量測定及び生体内ヒドロキシルラジカルのタンパク質フットプリント法
US20220313806A1 (en) 2021-03-25 2022-10-06 Iovance Biotherapeutics, Inc. Methods and compositions for t-cell coculture potency assays and use with cell therapy products

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US3826364A (en) * 1972-05-22 1974-07-30 Univ Leland Stanford Junior Particle sorting method and apparatus
US4094745A (en) * 1973-06-22 1978-06-13 John Scholefield Method of staining microscopic organisms
US4284412A (en) * 1979-07-13 1981-08-18 Ortho Diagnostics, Inc. Method and apparatus for automated identification and enumeration of specified blood cell subclasses
US4544546A (en) * 1980-04-21 1985-10-01 Abbott Laboratories Fluorescent nucleic acid stains
US4520110A (en) * 1981-10-06 1985-05-28 The Board Of Trustees Of The Leland Stanford Junior University Fluorescent immunoassay employing a phycobiliprotein labeled ligand or receptor
JPS59184862A (ja) * 1983-04-05 1984-10-20 ベクトン・ディッキンソン・アンド・カンパニ− 試料内細胞の複数副次集団を識別する方法と装置
US4585736A (en) * 1983-10-18 1986-04-29 The United States Of America As Represented By The United States Department Of Energy Flow cytometric measurement of total DNA and incorporated halodeoxyuridine
CA1254134A (en) * 1984-03-28 1989-05-16 Mount Sinai School Of Medicine Of The City University Of New York Specific binding flow cytometry method
US4661913A (en) * 1984-09-11 1987-04-28 Becton, Dickinson And Company Apparatus and method for the detection and classification of articles using flow cytometry techniques
US4727020A (en) * 1985-02-25 1988-02-23 Becton, Dickinson And Company Method for analysis of subpopulations of blood cells
JPH0663973B2 (ja) * 1985-08-07 1994-08-22 東ソー株式会社 免疫反応測定に用いる蛍光検出装置
CA1279008C (en) * 1985-10-11 1991-01-15 Smith Kline & French Canada Ltd. Methods and reagents for performing subset analysis
ZA867698B (en) * 1985-10-11 1987-07-29 Smithkline Beckman Corp Methods and reagents for performing subset analysis
US5016283A (en) * 1985-11-04 1991-05-14 Cell Analysis Systems, Inc. Methods and apparatus for immunoploidy analysis

Also Published As

Publication number Publication date
DE68910730T2 (de) 1994-05-19
US5057413A (en) 1991-10-15
EP0347039A2 (de) 1989-12-20
EP0347039B1 (de) 1993-11-18
ES2061987T3 (es) 1994-12-16
JPH02103464A (ja) 1990-04-16
EP0347039A3 (en) 1990-10-24
DE68910730D1 (de) 1993-12-23
JP2620810B2 (ja) 1997-06-18

Similar Documents

Publication Publication Date Title
ATE97500T1 (de) Verfahren zur unterscheidung zwischen intakten und beschaedigten zellen in einer probe.
DK296889A (da) Fremgangsmaade til analyse af cellekomponenter i en vaeske
ATE50647T1 (de) Verfahren zur quantifizierung von zellpopulationen bzw. subpopulationen sowie hierfuer geeignetes reagenz.
ATE190724T1 (de) Polymere phosphoniumsalze, die chemilumineszenzverstärkung von 1,2-dioxetanen hervorrufen, und deren verwendung.
DE3485653D1 (de) Verfahren und vorrichtung zur messung des reaktionsgrades zwischen antigenen und zell-leukozytenantikoerpern.
DE3786233D1 (de) Durchfluss-zytometrieverfahren zum klassifizieren von leukozyten und dazu angewandte reagenzien.
DE3875456D1 (de) Verfahren zur analyse haematopoietischer zellen in einer probe.
DE69016377D1 (de) Reagenz und Verfahren zur Verwendung desselben in der automatischen durchflusszytometrischen Bestimmung von mindestens einer Leukocyten-Subpopulation aus Vollblut.
DE3671534D1 (de) Verfahren zum nachweis von agglutinierten erythrozyten.
ATE63002T1 (de) Testverfahren unter verwendung eines in eine zelle aufnehmbaren fluoreszierenden farbstoffs.
DE69024955D1 (de) Ermittlung und quantifizierung mehrerer analyten unter verwendung von markierungstechniken
DE69826816D1 (de) Verfahren zum Nachweis und zur Identifizierung, Zählung und Bestätigung von zirkulierenden Krebszellen und/oder hämatologischen Vorläuferzellen in Vollblut
DE68923605D1 (de) Immunodiagnostisches Verfahren zum Nachweis und zur Bestimmung von Euterentzündung.
DK0603107T3 (da) Fremgangsmåde til samtidig kvantificering af de vigtigste typer af humane lymfocytter og deres deltyper
ATE100483T1 (de) Xanthenfarbstoffe.
ES2037659T3 (es) Metodos y reactivos para realizar analisis de subpoblaciones de particulas.
DK413688A (da) Differentiel binding af potentielt sensitive materialer til lymphocytter
ATE86672T1 (de) Nachweis und differenziierung von coxiella burnetit in biologischen fluessigkeiten.
EP0380019A3 (de) Fluoreszenz-Polarisationsimmunoassay für Methadon
TW375681B (en) Method for classifying and counting of juvenile leukocyte
ATE266100T1 (de) Verfahren zur analyse vom zellzyklus von zellsubpopulationen aus heterogenen zellproben
DE69007482D1 (de) Nachweis von schimmeln.
EP0089826A3 (de) Verfahren zur Spezifizierung serologisch ausgewählter Zell-Populationen
HU Crack-inclusion Interaction by Hybrid Dislocation-boundary Element Method
ES2017741B3 (es) Procedimiento para determinacion de paratormona.

Legal Events

Date Code Title Description
RER Ceased as to paragraph 5 lit. 3 law introducing patent treaties