ATE63002T1 - Testverfahren unter verwendung eines in eine zelle aufnehmbaren fluoreszierenden farbstoffs. - Google Patents

Testverfahren unter verwendung eines in eine zelle aufnehmbaren fluoreszierenden farbstoffs.

Info

Publication number
ATE63002T1
ATE63002T1 AT85306054T AT85306054T ATE63002T1 AT E63002 T1 ATE63002 T1 AT E63002T1 AT 85306054 T AT85306054 T AT 85306054T AT 85306054 T AT85306054 T AT 85306054T AT E63002 T1 ATE63002 T1 AT E63002T1
Authority
AT
Austria
Prior art keywords
cell
sbp member
abortable
fluorescent dye
assay methods
Prior art date
Application number
AT85306054T
Other languages
English (en)
Inventor
Chiu C Chang
Vartan Ghazarossian
Edwin F Ullman
Original Assignee
Syntex Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Syntex Inc filed Critical Syntex Inc
Application granted granted Critical
Publication of ATE63002T1 publication Critical patent/ATE63002T1/de

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/554—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being a biological cell or cell fragment, e.g. bacteria, yeast cells
    • G01N33/555—Red blood cell
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/531—Production of immunochemical test materials
    • G01N33/532—Production of labelled immunochemicals
    • G01N33/533—Production of labelled immunochemicals with fluorescent label
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/536—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase
    • G01N33/542—Immunoassay; Biospecific binding assay; Materials therefor with immune complex formed in liquid phase with steric inhibition or signal modification, e.g. fluorescent quenching
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/80—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving blood groups or blood types or red blood cells
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/80—Fluorescent dyes, e.g. rhodamine
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/805—Optical property
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/807—Apparatus included in process claim, e.g. physical support structures
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10—TECHNICAL SUBJECTS COVERED BY FORMER USPC
    • Y10S—TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y10S436/00—Chemistry: analytical and immunological testing
    • Y10S436/829—Liposomes, e.g. encapsulation

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • Hematology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Chemical & Material Sciences (AREA)
  • Urology & Nephrology (AREA)
  • Cell Biology (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • General Physics & Mathematics (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Pathology (AREA)
  • Mycology (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By The Use Of Chemical Reactions (AREA)
  • Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Luminescent Compositions (AREA)
AT85306054T 1984-08-28 1985-08-27 Testverfahren unter verwendung eines in eine zelle aufnehmbaren fluoreszierenden farbstoffs. ATE63002T1 (de)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US06/645,458 US4748129A (en) 1984-08-28 1984-08-28 Assay method employing fluorescent cell incorporative dye
EP85306054A EP0176252B1 (de) 1984-08-28 1985-08-27 Testverfahren unter Verwendung eines in eine Zelle aufnehmbaren fluoreszierenden Farbstoffs

Publications (1)

Publication Number Publication Date
ATE63002T1 true ATE63002T1 (de) 1991-05-15

Family

ID=24589110

Family Applications (1)

Application Number Title Priority Date Filing Date
AT85306054T ATE63002T1 (de) 1984-08-28 1985-08-27 Testverfahren unter verwendung eines in eine zelle aufnehmbaren fluoreszierenden farbstoffs.

Country Status (7)

Country Link
US (1) US4748129A (de)
EP (1) EP0176252B1 (de)
JP (1) JPH0692971B2 (de)
AT (1) ATE63002T1 (de)
AU (1) AU591636B2 (de)
CA (1) CA1261742A (de)
DE (1) DE3582643D1 (de)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5026905A (en) * 1984-08-28 1991-06-25 Syntex (U.S.A.) Inc. Fluorescent dyes
US4806488A (en) * 1985-09-06 1989-02-21 Syntex (U.S.A.) Inc. Ligand-receptor assays employing squarate dye compositions
US6956032B1 (en) 1986-04-18 2005-10-18 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
US5569587A (en) * 1986-04-18 1996-10-29 Carnegie Mellon University Method for labeling and detecting materials employing luminescent arysulfonate cyanine dyes
US5627027A (en) * 1986-04-18 1997-05-06 Carnegie Mellon University Cyanine dyes as labeling reagents for detection of biological and other materials by luminescence methods
CA1340806C (en) * 1986-07-02 1999-11-02 James Merrill Prober Method, system and reagents for dna sequencing
EP0261719A3 (de) * 1986-09-23 1990-10-10 Akzo N.V. Thermochemischlumineszierende Komplexe von Cyclodextrinen
US4891324A (en) * 1987-01-07 1990-01-02 Syntex (U.S.A.) Inc. Particle with luminescer for assays
AU607533B2 (en) * 1987-08-07 1991-03-07 Xoma Corporation Methods and compositions for mimicking antigenic determinants
US5256532A (en) * 1988-05-02 1993-10-26 Zynaxis Technologies, Inc. Methods, reagents and test kits for determination of subpopulations of biological entities
US5385822A (en) * 1988-05-02 1995-01-31 Zynaxis, Inc. Methods for detection and quantification of cell subsets within subpopulations of a mixed cell population
EP0430968B1 (de) * 1988-05-02 1996-11-20 PHANOS TECHNOLOGIES, Inc. Verbindungen, zusammensetzungen und verfahren zum binden von bio-affektions-substanzen an oberflächenmembranen von bioteilchen
CA1324499C (en) * 1988-06-23 1993-11-23 John Vorpahl Multiparameter particle analysis
US5375606A (en) * 1990-02-28 1994-12-27 Zynaxis, Inc. Bio-analytical separation method
US6593148B1 (en) 1994-03-01 2003-07-15 Li-Cor, Inc. Cyanine dye compounds and labeling methods
DE4429239A1 (de) * 1994-08-18 1996-02-22 Basf Ag Verfahren zur Fluoreszenz-Korrelations-Spektroskopie und Vorrichtung zu dessen Durchführung
US5776711A (en) * 1996-11-12 1998-07-07 The Regents Of The University Of California Simultaneous human ABO and RH(D) blood typing or antibody screening by flow cytometry
DE19903576C2 (de) 1999-01-29 2001-02-22 Bodenseewerk Perkin Elmer Co Quantitative Bestimmung von Analyten in einem heterogenen System
CA2309528C (en) 1999-06-08 2007-10-09 Ortho-Clinical Diagnostics, Inc. Simultaneous determination of forward and reverse abo blood group
DE10008373C2 (de) * 2000-02-23 2002-11-28 Helmut Adelsberger Verfahren und Vorrichtung zur Bestimmung der Ionenkanalaktivität
CN109917141B (zh) * 2019-03-19 2022-05-10 迪佰(厦门)生物科技有限公司 一种检测人abo血型试纸条、制备方法、检测方法及应用
CN110736832B (zh) * 2019-12-18 2020-04-10 首都医科大学附属北京友谊医院 一种抗体鉴定谱细胞的组合物

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4080264A (en) * 1976-03-01 1978-03-21 Massachusetts Institute Of Technology Immunoassay by light scattering spectroscopy
US4424201A (en) * 1978-11-28 1984-01-03 Rockefeller University Employment of a mereyanine dye for the detection of malignant leukocytic cells
US4342739A (en) * 1979-01-09 1982-08-03 Fuji Photo Film Co., Ltd. Novel material for immunological assay of biochemical components and a process for the determination of said components
US4319882A (en) * 1980-02-21 1982-03-16 Yash Sharma Method for detecting immunological agglutination and biochemical agent therefor
FR2482309A1 (fr) * 1980-04-21 1981-11-13 Fax Gie Procede de detection de la presence d'antigenes d'un groupe sanguin dans une coupe histologique, et produits pour mettre en oeuvre ce procede
EP0039195B1 (de) * 1980-04-28 1986-06-18 Montefiore Hospital and Medical Center Verfahren zur Feststellung von Antikörpern
US4564598A (en) * 1982-07-12 1986-01-14 Syntex (U.S.A.) Inc. Limited volume method for particle counting
US4550017A (en) * 1982-10-15 1985-10-29 Syntex (U.S.A.) Inc. Fluorescence screening for blood typing
US4584277A (en) * 1983-04-05 1986-04-22 Syntex (U.S.A.) Inc. Fluorescent multiparameter particle analysis
US4713324A (en) * 1983-09-01 1987-12-15 Technicon Instruments Corporation Inverted latency specific binding assay
JPS61502280A (ja) * 1984-05-31 1986-10-09 ク−ルタ−・エレクトロニクス・インコ−ポレ−テッド 白血球のクラスおよびサブクラスの同定、計数並びに試験方法およびそれに用いる試薬系
US4707441A (en) * 1984-08-06 1987-11-17 Technicon Instruments Corp. Binding assays in automated apparatus with liposome compatible surfactants

Also Published As

Publication number Publication date
EP0176252A2 (de) 1986-04-02
CA1261742A (en) 1989-09-26
EP0176252A3 (en) 1987-07-29
DE3582643D1 (de) 1991-05-29
JPS6162862A (ja) 1986-03-31
AU4682085A (en) 1986-03-06
JPH0692971B2 (ja) 1994-11-16
AU591636B2 (en) 1989-12-14
US4748129A (en) 1988-05-31
EP0176252B1 (de) 1991-04-24

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Legal Events

Date Code Title Description
UEP Publication of translation of european patent specification
REN Ceased due to non-payment of the annual fee